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All Journal Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Jurnal Respirasi (JR) Jurnal Layanan Masyarakat (Journal of Public Service) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Indian Journal of Forensic Medicine & Toxicology International Journal of Social Service and Research Jurnal Ilmiah Kesehatan (JIKA) Jurnal Widya Medika

Rebekah Setiabudi, Rebekah

Department Of Medical Microbiology, Faculty Of Medicine, Universitas Airlangga, Surabaya, Indonesia

Author-ID : 563212

Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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CELLULAR IMMUNITY ACTIVATION METHOD BY STIMULATING RD1 COMPLEX PROTEINS AS VIRULENCE MARKER ON Mycobacterium tuberculum TO ESTABLISH DIAGNOSIS ON TUBERCULOSIS AND LATENT TUBERCULOSIS INFECTION Setiabudi, Rebekah; Mertaniasih, Ni Made; Didik Handijatmo, Didik; Setyoningrum, Retno Asih
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1205

This study was intended to invent a simpler and more affordable method to establish diagnosis on Tuberculosis (TB) and Latent Tuberculosis infection (LTBI). Similar to âQuantiferon TB Gold In Tubeâ (QFT-GIT) and T.SPOT.TB methods, the researchers also utilized âearly secreted antigenic target 6kDaâ (ESAT-6) and âcultur filtrate protein 10kDaâ (CFP-10) proteins to be induced on the specimen. ESAT-6 and CFP-10 are commercial products used to induce interferon gamma (INF-Î³) which were to be read using sophisticated and expensive equipment. This study was intended to conduct an analysis on effective cocktail protein modification, i.e. ESAT-6, CFP-10 and Ag85A/B/C, with high validity to detect cellular immunity activity through in vitro examination on peripheral blood monocyte cells of Tuberculosis-suspected patients or patients with latent tuberculosis infection. Peripheral Blood Monocyte Cells (PBMCs) activity on children tuberculosis patient or Latent Tuberculosis Infection (LTBI), adult tuberculosis patient or LTBI, which induced by cocktail protein modification and not induced, were analyzed microscopically. The activity of PBMCs on children and adult tuberculosis patient or LTBI induced by RD1 secretory proteins: ESAT-6, CFP-10, Ag85A/B/C was higher compared to PBMCs which had not been induced by the secretory proteins. Cellular debris and monocyte cells with abnormal shapes were found on PBMCs which had been induced by RD1 secretory proteins at 8 th day after culture.

The Antibacterial Effect of Ethanol Extract of Garlic (Allium sativum L.) on Methicillin Resistant Staphylococcus aureus (MRSA) In Vitro Utami Meilanie Putri; Maftuchah Rochmanti; Manik Retno Wahyunitisari; Rebekah J. Setiabudi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14918

Objective: To determine the antibacterial activity of ethanol extract of garlic against Methicillin-ResistantStaphylococcus aureus. Method: Dilution test was performed on 9 different concentration of ethanol extractof garlic (1024, 512, 256, 128, 64, 32, 16, 8, 4 mg/mL) dissolved in DMSO 10% and MRSA suspensionon Mueller Hinton broth medium to determine the Minimum Inhibitory Concentration. The MinimumBactericidal Concentration was determined by the result of streaking of MRSA inoculation on the nutrientagar plate. Results: The observation through Mueller Hinton broth medium has shown turbidity both beforeand after incubation because of garlic ethanol extract color which is blackish-brown and thick, while in thenutrient agar medium, MRSA bacteria grew on the media with concentrations 4, 8, 16, 32, 64, & 128 mg/mL and unable to grow at concentrations of 256, 512, and 1024 mg/mL. Conclusion: The MIC of ethanolextract of garlic on MRSA could not be determined, and the MBC in this study was 256 mg/mL.

The Effect of Pre-Operating Antibiotic Prophylaxis on Post C-Section Infection In RSUD DR. Soetomo Period January 2021 – June 2021 Mariska Nuriana; Sri Ratna Dwiningshih; Danti Nur Indiastuti; Rebekah J. Setiabudi
International Journal of Social Service and Research Vol. 2 No. 1 (2022): International Journal of Social Service and Research (IJSSR)
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/ijssr.v2i1.68

Cesarean section has some risk of around 90% post operative morbidity caused by Surgical Site Infection. The use of prophylactic antibiotics is one of the prevention of surgical wound infections with a decrease of about 30-65%. This study will use a prospective and cross-sectional study design, to determine the relationship between the use of preoperative prophylactic antibiotics on the prevention of infection after cesarean section. The results is From 72 cesarean section patients, it can be concluded that the age group of most cesarean section patients in this study was the age group of 20-35 years. The highest BMI group in cesarean section patients was with BMI > 30 by 43.1%. The most indications for cesarean section patients are patients with a history of previous cesarean section with a total of 30 people. The most prophylactic antibiotic use was cefazolin as much as 95.8% The incidence of infection in patients using cefazolin prophylactic antibiotics was 3 patients out of 69 patients, while the incidence of infection who did not use cefazolin was 1 patient out of 3 patients. There is a relationship between the use of preoperative prophylactic antibiotics on the prevention of infection after cesarean section

PENYULUHAN DAN PELATIHAN DENGUE PADA IBU SERTA DETEKSI DINI INFEKSI DENGUE PADA PASIEN DENGAN SUSPEK INFEKSI DENGUE DI TULUNGAGUNG, JAWA TIMUR Juniastuti Juniastuti; Lynda Rossyanti; Manik R Wahyunitisari; Achmad Ardianto; Rebekah J Setiabudi; Suhintam Pusarawati; Dewi Setyowati; Putri S Wulandari
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 1 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i1.2020.230-236

Tulungagung Regency in East Java, is a Dengue endemic area, but the movement of 1 house 1 jumantik / larva monitor (Government program for controlling Dengue vectors), which involves every family in the inspection, monitoring and eradication of mosquito larvae, has not yet been implemented. The objectives of this community service activity are 1) increasing maternal knowledge about Dengue, 2) increasing maternal efforts in eradicating Aedes mosquito larvae (Dengue vectors) and 3) improving mother's skills in monitoring mosquito larvae in Karangrejo District; and 4) determine the proportion of early dengue infection at the Karangrejo Health Center. The method used is counseling, training and laboratory examinations (rapid test with the NS1 Dengue kit). This community service obtained the conclusion of counseling can increase the knowledge of dengue in mothers, counseling can increase maternal efforts in eradicating mosquito larvae, training to improve the skills of mothers in monitoring mosquito larvae, there are 10.8% of patients with symptoms of dengue infection who show positive NS1.abstrakKabupaten Tulungagung di Jawa Timur, merupakan daerah endemis Dengue, namun gerakan 1 rumah 1 jumantik/juru pemantau jentik (program Pemerintah untuk pengendalian vektor Dengue) yaitu yang melibatkan setiap keluarga dalam pemeriksaan, pemantauan, dan pemberantasan jentik nyamuk, masih belum banyak diterapkan. Tujuan kegiatan pengabdian masyarakat ini adalah 1) meningkatkan pengetahuan ibu tentang Dengue, 2) meningkatkan upaya ibu dalam memberantas tempat perindukan jentik nyamuk Aedes (vektor Dengue) dan 3) meningkatkan ketrampilan ibu dalam memantau jentik nyamuk di Kecamatan Karangrejo; serta 4) menentukan proporsi kejadian infeksi Dengue secara dini di Puskesmas Karangrejo. Metode yang digunakan adalah penyuluhan, pelatihan dan pemeriksaan laboratorium (uji cepat dengan kit NS1 Dengue). Pengmas ini memperoleh simpulan penyuluhan dapat meningkatkan pengetahuan dengue pada ibu, penyuluhan dapat meningkatkan upaya ibu dalam memberantas tempat perindukan jentik nyamuk, pelatihan meningkatkan ketrampilan ibu dalam memantau jentik nyamuk, ada 10,8% pasien dengan gejala infeksi Dengue yang menunjukkan NS1 positif.

PENINGKATAN PENGETAHUAN DAN KEAHLIAN DIAGNOSIS MIKROSKOPIS BTA PETUGAS LABORATORIUM DI SORONG PADA ERA PANDEMI COVID-19 Rebekah J Setiabudi; Ni Made Mertaniasih; Manik Retno Wahyunitisari
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 5 No. 1 (2021): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v5i1.2021.111-115

ABSTRAKPenyakit Tuberkulosis (TB) Paru merupakan salah satu penyebab morbiditas dan mortalitas penting di dunia. Indonesia masih merupakan salah satu negara yang mempunyai beban TB yang terbesar diantara 5 negara yaitu: India, China, Nigeria dan Pakistan. Kota dan Kabupaten Sorong merupakan salah satu daerah di Propinsi Papua Barat dimana TB masih menjadi salah satu permasalahan kesehatan. Pemeriksaan mikroskopis TB dengan metode Ziehl Neelsen, menurut WHO, masih merupakan sarana diagnostik yang sensitif, spesifik, praktis, dan juga murah. Belum terstandardisasinya kinerja petugas laboratorium TB juga menjadi kendala tersendiri dalam diagnosis TB dengan metode Ziehl Neelsen. Munculnya pandemi Covid-19 yang melanda dunia termasuk Indonesia, membuat berbagai kegiatan menjadi tertunda. Kegiatan Pengabdian Kepada Masyarakat di tengah pandemi Covid-19 ini terpaksa dilakukan dalam bentuk “workshop online”. Dalam meningkatkan pengetahuan digunakan aplikasi zoom untuk kuliah penyegaran, memberikan informasi terbaru terkait TB di Indonesia dan pengetahuan tambahan lainnya. Sedangkan dalam meningkatkan keahlian dilakukan praktek pembuatan sediaan mikroskopis TB dan latihan pembacaan sediaan mikroskopis TB yang dilaksanakan secara offline di laboratorium Mikrobiologi Rumah Sakit JP Wanane, yang dimonitor dan dibimbing oleh Dokter Spesialis Mikrobiologi Klinik yang bertugas di sana. Melalui kegiatan Pengabdian Kepada Masyarakat yang dilakukan oleh Universitas Airlangga ini, diharapkan dapat membangkitkan lagi semangat, melakukan penyegaran serta menambah pengetahuan dan ketrampilan bagi tenaga kesehatan di daerah untuk dapat melakukan tugasnya dengan baik sehingga Program Pengendalian Infeksi Tuberkulosis dapat kembali berjalan sebagaimana seharusnya. Kata kunci : tuberkulosis, pemeriksaan mikroskopis, sorong, pandemi Covid-19 ABSTRACTPulmonary Tuberculosis (TB) is one of the most important causes of morbidity and mortality in the world. Indonesia is still one of the countries with the largest burden of TB among 5 countries, namely: India, China, Nigeria and Pakistan. Sorong City is one of the areas in Western Papua, Indonesia, where TB is still a health problem. According to WHO, microscopic examination of TB by the Ziehl Neelsen method is still a sensitive, specific, practical, and inexpensive diagnostic tool. The unstandardized performance of TB laboratory personnel has also become an obstacle in itself in diagnosing TB with the Ziehl Neelsen method. The emergence of the Covid-19 pandemic has delayed various activities. Community Service Activities in the midst of the Covid-19 pandemic had to be carried out in the form of an "online workshop". In increasing knowledge, the zoom application is used for refresher lectures, providing the latest information related to TB in Indonesia and other additional knowledge. Meanwhile, in increasing expertise, the practice of making TB microscopic preparations and reading exercises for TB microscopic preparations was carried out offline in the Microbiology laboratory of the JP Wanane Hospital, which was monitored and guided by the Clinical Microbiology Specialist who served there. Through this Community Service activity carried out by Airlangga University, it is hoped that it can revive enthusiasm, refresh and increase knowledge and skills for health workers in the regions to be able to carry out their duties properly so that the Tuberculosis Infection Control Program can resume running as it should.Keyword : tuberculosis, microscopic examination, Sorong, Covid-19 pandemic

Profile of Pulmonary Tuberculosis Patients with Type 2 Diabetes Mellitus in Pulmonary Department Dr. Soetomo General Hospital Surabaya Ayu Rahmanita Putri Soetrisno; Rebekah Juniati Setiabudi; Laksmi Wulandari
Jurnal Respirasi Vol. 6 No. 2 (2020): May 2020
Publisher : Faculty of Medicine Universitas Airlangga

Background: Pulmonary tuberculosis (TB) is the most important infectious disease, caused by Mycobacterium tuberculosis (MTB). Indonesia is the third country with the most prevalent TB cases in the world. Not all people who were exposed to MTB will become active TB, because the immune response is the main determinant. Immunocompromised person, such as patients who have type 2 diabetes mellitus (DM) as a comorbid are more prone to develop active TB infection. This study aimed to analyze the profile of pulmonary TB patients with type 2 DM in Pulmonary Department Dr. Soetomo General Hospital Surabaya.Methods: This was an observational descriptive study with a cross-sectional design. The research data were obtained from the medical records of TB patients with type 2 DM in Pulmonary Department Dr. Soetomo General Hospital Surabaya in January - December 2017.Results: Out of a total of 414 hospitalized patients, 74 patients with pulmonary TB were found with type 2 DM. Only 53 patients fulfilled the inclusion criteria. Gene Xpert results were sensitive (66.7%), previous TB treatment history (54.7%), unregulated type 2 DM (88.4%), RBG with the average of 311.83 mg/dl, PPBS with the average of 263.31 mg/dl, FBG with the average of 238 mg/dl, and HbA1C > 10% (68%).Conclusion: The majority of the patients of pulmonary TB with type 2 DM have average high blood glucose and unregulated DM.

First-Line Anti-Tuberculosis Drug Resistance Pattern Ayurveda Zaynabila Heriqbaldi; Rebekah Juniati Setiabudi; Resti Yudhawati
Jurnal Respirasi Vol. 8 No. 1 (2022): January 2022
Publisher : Faculty of Medicine Universitas Airlangga

Introduction: An important concern regarding TB currently is the case of anti-TB drug resistance; hence research on resistance patterns and risk factors is essential. This study aimed to identify the pattern of first-line anti-TB drug resistance. Methods: This descriptive retrospective study was conducted at MDR-TB polyclinic Dr. Soetomo General Hospital, Surabaya, in 2017-2019. Only complete medical records were included. The variables were first-line anti-TB drug resistance (isoniazid, rifampicin, ethambutol, streptomycin) and patients’ characteristics (age, gender, treatment history, and comorbidities). The drug resistance test was performed using certified culture methods. Results: Among 239 patients, the incidences of resistance to H, R, E, and S were 79.08%, 94.14%, 25.94%, and 20.08%, respectively. The most common patterns were HR (42.26%), R (18.83%), and HRE (12.55%). The largest age group was 45-54 years old (38%). The dominant gender was male (56.49%). The most treatment history category was relapsed patients (48%) and there were more patients with comorbidity (57%). Conclusion: The highest incidence rate of resistance was rifampicin and the most common resistance pattern was HR. Most of the patients were of working age, male, relapse patients, and had comorbidities. An appropriate TB therapy treatment plays an important role in preventing resistance.

Penerapan Higiene dan Sanitasi Lingkungan pada Pasien Diare Balita di Puskesmas Surabaya Anindita Rehana; Rebekah Juniati Setiabudi; Sulistiawati Sulistiawati; Manik Retno Wahyunitisari
Jurnal Ilmiah Kesehatan (JIKA) Vol. 3 No. 1 (2021): Volume 3, Issue 1 April 2021
Publisher : Sarana Ilmu Indonesia (salnesia)

Surabaya has the most cases of diarrhea in East Java, with the highest detection targets in Gading Health Center (2346 cases) and Mojo Health Center, with diarrhea cases handled by 1322 cases. This study aimed to describe hygiene and environmental sanitation in under-five diarrhea patients in the working area of Surabaya Health Center. This study used a survey method, descriptive approach, interviews, and questionnaires in October 2019-March 2020. The results showed 62,2% of under-fives ‘drinking water sources were ineligible, 86,5% of mothers’ handwashing behavior were ineligible, 67,6% of the use of serving hoods were ineligible, 100% of food ripeness level were eligible, 59,5% of the distance between well and septic tank were eligible, 100% of latrines were eligible, 89,2% of sewerages were ineligible, and 83,8% of waste disposal facilities were ineligible. This research concluded that drinking water sources, handwashing behavior, serving hoods, sewerages, and waste disposal facilities were ineligible. The problems can be solved by consuming branded gallons of water / well water or refillable water that is boiled before consumption, improving how to wash hands with soap and running water, covering food using a serving hood, use the cover to close the sewerage, and throwing the trash into the temporary shelter (TPS) as much as two times a week or less than 3x24 hours.

Clinical Profile and Treatment of Acne Vulgaris Patients M. Yulianto Listiawan; Farah Meriana Fajrin; Rahmadewi Rahmadewi; Afif Hidayati; Sawitri Sawitri; Diah Mira Indramaya; Rebekah Juniati Setiabudi; Maya Wardiana
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 34 No. 3 (2022): DECEMBER
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V34.3.2022.156-161

Background: Acne vulgaris (AV) is not a deadly condition may affect one’s quality of life. Treatment of AV includes topical and oral, depending on the severity. Antibiotics as oral treatment should not be given alone because it can precipitate resistance. Purpose: To describe the clinical characteristics of AV in a tertiary hospital in Indonesia and evaluate the treatment given. Methods: This is a descriptive, retrospective study with a cross-sectional design. The study subject was classified based on severity according to Plewig and Kligman’s acne grading. Further evaluation was made according to the type of lesion, the risk factortopical and oral treatment in subjects with grades 2, 3, and 4. This research was conducted at the Cosmetic Medic Division, Dermatology and Venereology Outpatient Clinic at Dr. Soetomo General Academic Hospital Surabaya based on medical records from the period of 2017 to 2019. Result: From 2017 to 2019, there were 525 (1.1%) new AV patients out of a total of 45,754 new patients. AV was the major diagnosis in the Cosmetic Medic division. AV grades 2, 3, and 4 were found in 167 patients (32.0%). The most common lesion was papulopustular (75.4%) and the most common risk factor was hormonal (58.7%). All the patients received tretinoin, clindamycin gel 1.2%, and sunscreen for topical treatment. Doxycycline was the most common oral antibiotic used (98.2%) Conclusion: The most common AV grade in this study was mild AV. Oral antibiotics were given to moderate-to-severe AV patients in combination with topical treatment to prevent resistance.

Bacterial and Antibiogram Profile of Urinary Tract Infection Patients in a Tertiary Hospital, Surabaya, Indonesia Mahrumi Dewi Tri Utami; Manik Retno Wahyunitisari; Nunuk Mardiana; Rebekah Juniati Setiabudi
Folia Medica Indonesiana Vol. 58 No. 3 (2022): September
Publisher : Universitas Airlangga

Highlights: Most urinary tract infections are caused by Gram-negative bacteria with E. coli being the most common bacteria. Antibiotics with the highest susceptibility for Gram-negative bacteria were ertapenem, meropenem, amikacin, and imipenem. Antibiotics with the highest susceptibility for Gram-positive bacteria mainly were susceptible to chloramphenicol,streptomycin, vancomycin, rifampin, tigecycline, teicoplanin, and ampicillin. Abstract: Urinary tract infection (UTI) is one of the most common bacterial infections. Inappropriate antibiotic use for UTI treatment may lead to antibiotic resistance. This study aimed to provide an updated bacterial and antibiogram profile from urine specimens of patients diagnosed with UTI. This study was a retrospective study using urine culture and antibiotic susceptibility test results obtained from Clinical Microbiology Laboratory in a tertiary general hospital in Surabaya, Indonesia for a two-month period patients. Most aged more than and/or equal to 59 years, in both sexes. Gram-negative bacteria, particularly , was being the most between June to July 2019. There were 215 patients with significant urine culture results of 54.4% from Escherichia coli female bacteria were carbapenem antibiotics and amikacin, while teicoplanin and vancomycin were some antibiotics susceptible to gramcommon bacteria that caused UTI, followed by K. pneumoniae. Some antibiotics with the highest susceptibility to gram-negative positive bacteria. This study result indicated that there was an urge to conduct local antibiogram profile investigation due to the low susceptibilities shown in recent empirical therapy recommendations, such as trimethoprim sulfamethoxazole, fluoroquinolone, nitrofurantoin, and fosfomycin.

Co-Authors Achmad Ardianto Adhima, Fauziah Afif Hidayati Agung Dwi Wahyu Widodo Alimsardjono, Lindawati Anindita Rehana Ayu Rahmanita Putri Soetrisno Ayurveda Zaynabila Heriqbaldi Azzahra, Safira Deby Kusumaningrum Dewi Setyowati Diah Mira Indramaya Didik Didik Handijatmo, Didik Dwiyanti Puspitasari, Dwiyanti Eko Budi Koendhori, Eko Budi Evy Ervianti Farah Meriana Fajrin Hammam Izza Rofiqi Hasan, Helmia Indiastuti, Danti Nur Juniastuti Juniastuti Khairunnida Rahma Laksmi Wulandari M. Yulianto Listiawan Mahrumi Dewi Tri Utami Manik R Wahyunitisari ManikRetno Wahyunitisari Mariska Nuriana Marsha Zahrani Maya Wardiana Ni Made Mertaniasih Nunuk Mardiana, Nunuk Pepy Dwi Endraswari, Pepy Dwi Putri S Wulandari Rahmadewi Rahmadewi Resti Yudhawati Retno Asih Setyoningrum Risky Vitria Prasetyo Rochmanti, Maftuchah Rochmanti Rossyanti, Lynda Sawitri Sawitri Silvia Sutandhio Sri Ratna Dwiningshih Suhintam Pusarawati Sulistiawati Sulistiawati Titiek Sulistyowati Utami Meilanie Putri Wahyunitisari, Manik Retno Warella, Juen Carla

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