Article Per Year (5 Year)
p-Index From 2020 - 2025
2.083
P-Index
This Author published in this journals
All Journal BALI MEDICAL JOURNAL (BMJ) Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Ilmu dan Teknologi Kesehatan J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Medical and Health Science Journal Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Ilmiah Kesehatan Majalah Biomorfologi (Biomorphology Journal) Jurnal Kesehatan Tambusai
Nurul Wiqoyah, Nurul
Departement Of Microbiology, Faculty Of Medicine, Universitas Airlangga, Surabaya, Indonesia
Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
SPECIFIC GYRB SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATED FROM SPUTUM OF PULMONARY TUBERCULOSIS PATIENTS IN INDONESIA Mertaniasih, Ni Made; Wiqoyah, Nurul; Kusumaningrum, Deby; Soedarsono, S.; Perwitasari, Agnes Dwi Sis; Artama, Wayan Tunas
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (790.131 KB)

Abstract

Background: Indonesia have many different geographic areas which could be various on the variant strains of Mycobacterium tuberculosis. The gyrB gene codes GyrB protein as sub unit compound of Gyrase enzyme that functioning in multiplication of bacteria. Detection of gyrB gene could be a marker of active multiplication of viable bacteria in the specimen from patients; and some of the DNA sequence regions were conserved and specific in the strain of Mycobacterium tuberculosis that would be a marker for identification. This research aims to analyze the sequence of gyrB gene of Mycobacterium tuberculosis clinical isolates from sputum of pulmonary TB patients in Indonesia, and determine the specific region. Method: Mycobacterium tuberculosis clinical isolates have been collected from sputum of the patients with pulmonary TB that live in some area in Indonesia. Isolation and identification of Mycobacterium tuberculosis clinical isolates using standard culture method; sequence analysis using PCR-direct sequencing of the part bases region of gyrB. Results: this study revealed that nucleotide sequence on a fragment 764 bases of gyrB gene Mycobacterium tuberculosis strains among clinical isolates almost identically to a wild type strain Mycobacterium tuberculosis H37Rv and subspecies member of Mycobacterium tuberculosis complex (MTBC), with a little difference of SNPs; there are many difference nucleotide sequence with MOTT and Gram positive or negative bacteria, except Corynebacterium diphtheria identically with MTBC. Conclusion: the gyrB sequence in Mycobacterium tuberculosis strains among these clinical isolates from sputum of pulmonary TB patients in Indonesia have the conserved specific DNA region that almost identically with wild type strain H37Rv and MTBC.
Effectiveness of Black Honey as an Antiinflammatory Substance in Rat’s Wound Infected by Staphylococcus aureus Fanny Gunawan; Willy Sandhika; Nurul Wiqoyah
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 33 No. 1 (2021): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V33.1.2021.13-18

Abstract

Background: Black honey contains higher flavonoids, phenols, and minerals, which serves as antiinflammatory agents and promote faster wound healing. Purpose: This study aimed to determine the effectiveness of black honey on macrophage cells and blood vessels in rats’ wounds infected with Staphylococcus aureus. Methods: This was an experimental study with a post-test only control group design. This study involved 24 rats divided into 4 groups. The treatment was carried out for 5 days. The rats in the K1 group were wounded (cut) and given aquadest. The rats in the K2 group were wounded (cut), infected with Staphylococcus aureus, and given aquadest. The rats in the P1 group were wounded (cut), infected with Staphylococcus aureus, and treated with 2ml of black honey topically. The rats in the P2 group were wounded (cut), infected with Staphylococcus aureus, and treated with2ml of black honey orally. Observations were made histopathologicaly using hematoxylin-eosin (HE) staining by calculating the number of macrophage and blood vessels, and further using One-Way ANOVA. Result: The number of macrophages and blood vessels between groups was significantly different. The K2 group had the highest mean number of macrophage cells and blood vessels, and the K1 group was the least. The mean number of macrophage cells in the P1 group was more than the K1 group and less than the K2 and P2 groups, both significantly. Conclusion: Black honey was effective in reducing inflammation in wounds infected by Staphylococcus aureus.
Prevalence of Escherichia Coli, Salmonella Sp, Staphylococcus Aureus Bacteria in Chicken Meat of Traditional Market Surabaya City Ulfa Dwi Karisma; Nurul Wiqoyah; Suhintam Pusarawati
Jurnal Ilmu dan Teknologi Kesehatan Vol 8 No 2 (2021): Maret 2021
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32668/jitek.v8i2.510

Abstract

Foodborne disease is a disease caused by contaminated food. Chicken meat is very susceptible to contamination by bacteria. Escherichia coli, Salmonella sp, and Staphylococcus aureus are types of bacteria found in chicken meat. The purpose of this study was to see how many Escherichia coli, Salmonella sp, and Staphylococcus aureus colonies in raw chicken meat in traditional markets in Surabaya City. The research sample used was 30 samples of chicken meat taken in 5 traditional markets in Surabaya. Six chicken meat sellers were taken with 1 sample and put in the sterile tube from each market. The test was carried out using the Total Plate Count (TPC) method. The TPC test results showed that all samples were contaminated with E. coli, with the most colony being 2.2X10-7 CFU/g while the minor colony was 5X10-4 CFU/g. There are two samples contaminated with Salmonella sp, in the G market and the W market, with code D6 4.7X10-6 CFU/g and code E5 1X10-6 CFU/g. Meanwhile, S. aureus was only found in the W market with sample codes E1 & E6, and each sample had a colony of 2.7X 10-7 CFU/g and 2X10-5 CFU/g.
Antimicrobial Activity of Ethanol Extract of Centella asiatica Leaves on Proteus mirabilis, Proteus vulgaris, and Yersinia enterocolitica in vitro Salwa Putri Qurrotuaini; Nurul Wiqoyah; Arifa Mustika
Molecular and Cellular Biomedical Sciences Vol 6, No 3 (2022)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v6i3.266

Abstract

Background: Centella asiatica leaves ethanol extract (CALEE) has higher concentration compared to other structures within the plant. The extract contains alkaloids, saponins and flavonoids, which play an active role as antioxidant and antibacterial. Current study aimed to determine the effect of CALEE on Proteus mirabilis, Proteus vulgaris, and Yersinia enterocolitica, Gram-negative bacteria that cause diarrhea. Materials and methods: Simplicia of C. asiatica leaves was dissolved in 96% ethanol and macerated to get condensed extract, which then produced a concentration of 1 g/mL. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined to assess the effectiveness of CALEE on P. mirabilis, P. vulgaris, and Y. enterocolitica. MIC was determined through serial dilution test with Mueller Hinton broth media. After incubation, the bacteria were streaked on nutrient agar or McConkey agar to determine the MBC. Results: The MIC value of CALEE could not be determined since the color of CALEE was dark, hence the turbidity could not be compared. CALEE had the same MBC value (0.25 g/mL) in all bacteria species used in this study. Conclusion: CALEE is effective against Gram-negative bacteria, such as P. mirabilis, P. vulgaris, and Y. enterocolitica. Further research is needed, especially in vivo experiments and evaluation of the cytotoxicity effect of CALEE. Keywords: Centella asiatica, Proteus mirabilis, Proteus vulgaris, Yersinia enterocolitica, antibacterial
Antibiotic-Producing Streptomyces sp. Isolated from the Soil of a Mangrove Ecosystem Wiwin Retnowati; Ni Made Mertaniasih; Marijam Purwanta; Nurul Wiqoyah; Atika; Sekar Maharani; Wilda Mahdani
Folia Medica Indonesiana Vol. 59 No. 3 (2023): September
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i3.45806

Abstract

Highlights: 1. The unexplored soil of mangrove ecosystems in Surabaya, Indonesia, has the potential to be home to biodiversity,including Streptomyces sp. that can produce antibiotics.2. Streptomyces sp. has antibacterial properties against Gram-positive and Gram-negative bacteria, and the duration ofincubation plays a critical role in regulating the antibacterial activity.   Abstract A mangrove ecosystem in Surabaya, Indonesia, has a high salinity, pH, potassium, phosphorus, and nitrate contents. This ecosystem comprises a mixture of sand, dust, mud, and clay, which has the potential to be a conducive environment for the isolation of Streptomyces. The importance of Streptomyces in biotechnology lies in its ability to produce bioactive secondary metabolites, which represent a valuable reservoir of antibiotics. This research aimed to assess the antibiotic activity exhibited by Streptomyces sp. isolated from the soil of a mangrove ecosystem in Wonorejo, Surabaya, Indonesia. The analysis focused on the potential of Streptomyces sp. to produce antibiotics that work against Gram-positive bacteria (i.e., Staphylococcus aureus ATCC 25923 and Bacillus subtilis) as well as Gram-negative bacteria (i.e., Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Salmonella Typhimurium). The antibacterial activity test was conducted using the modified agar diffusion method. Observations were performed to identify any clear zone formation around the Streptomyces sp. agar colonies with a diameter of 0.8 cm and a height of 3 mm. The clear zone diameter was measured every 24 hours during the 10-day incubation period to assess the diversity of antibacterial activity. The antibacterial profile of Streptomyces sp. exhibited varying levels of activity against different bacterial strains in the tests conducted. The inhibition zone diameters demonstrated the highest levels of activity in Bacillus subtilis (15.9 mm) on day 7, Staphylococcus aureus (27.6 mm) on day 2, Pseudomonas aeruginosa (24.3 mm) on day 7, Escherichia coli (29.2 mm) on day 5, and Salmonella Typhimurium (27.5 mm) on day 7. The results indicated that Streptomyces sp. had inhibitory effects against Gram-positive bacteria as well as Gram-negative bacteria. In conclusion, Streptomyces sp. is a source of biodiversity found in the soil of mangrove ecosystems and has the ability to produce antibiotics.
Profil Spesies Candida Dan Pola Kepekaan Antijamur Pada Pasien Candidemia Darah di Unit Mikrobiologi Klinik RSUD Dr.Soetomo periode 2020-2023 Wardatun Faizah; Nurul Wiqoyah; Evy Ervianti; Pepy Dwi Endraswari
Jurnal Kesehatan Tambusai Vol. 5 No. 1 (2024): MARET 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v5i1.23799

Abstract

Candida spp fungal infections. can attack the circulatory system (can be known as candidemia or blood infection by the fungus Candida spp.). Patients in the intensive care unit (ICU) or emergency unit (IGD) with long-term air-conditioned conditions resulting in Candida spp. can grow more easily and increase the risk of fungal infections. This study aims to evaluate the profile of Candida spp. as well as patterns of sensitivity to antifungals in patients with blood candidemia in the Clinical Microbiology Unit of Dr. RSUD. Soetomo during the 2020-2023 period. This research method involves analysis of medical record data of patients diagnosed with blood candidemia, with a retrospective descriptive research type. The research samples were isolate data, used were clinical specimens that can cause candidiasis in the vagina, oral cavity and urogenital tract. The sample size in this study was as large as the data from isolates originating from a total of clinics in the time period. The results of this study provide a comprehensive picture regarding the distribution of Candida spp. the most commonly encountered, as well as the pattern of sensitivity to certain antifungals in patients with blood candidemia in the Dr. Hospital environment. Soetomo. It is hoped that the information obtained from this study will provide better guidance in the management and selection of appropriate therapy for patients affected by blood candidemia in the future.
Molecular Diagnostic Tools for Treponema pallidum Setyarini, Wahyu; Wiqoyah, Nurul; Muhammad Ansori
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 3 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i3.44506

Abstract

Syphilis, a common sexually transmitted disease, is caused by Treponema pallidum subsp. pallidum. Owing to the chameleonic behavior of syphilis, ocular involvement still presents a therapeutic problem. Direct detection of Treponema pallidum in the vitreous offers a potential diagnostic method because serodiagnosis has considerable limitations. The worldwide identification of T. pallidum substypes has occurred since the advent of molecular typing approaches. The purpose of this article is to provide more information on the development of a molecular approach for Treponema pallidum detection. A body of literature was gathered using automated database searches in Google Scholar, PubMed, and ScienceDirect. Although prior studies have focused on other genes, such as polA, 16S RNA, and the whole genome, there are still some that use the study of the arp and T. pallidum repeat (tpr) genes to subtype. Whole blood, vaginal ulcers, skin biopsies, and other samples can be used in molecular methods. Comparing quantitative reverse trascription-polymerase chain reaction (qRT-PCR) to traditional methods, such as reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody (IFA) assay, and virus isolation, qRT-PCR has the advantage of being faster and more sensitive. Quick molecular methods, particularly polymerase chain reaction (PCR) results, will enable early detection of primary, secondary, and latent syphilis, which will lead to prompt treatment and prevention of disease progression as well as a reduction in the amount of time that the patient's sexual partners are exposed to the illness.
The Comparison of Breast milk from Mother with Preterm Infant at 4o C Temperature in 0, 24 and 48 Hours on Bacterial Growth Saputra, Pandit Bagus Tri; Rochmah, Nur; Wiqoyah, Nurul
Biomolecular and Health Science Journal Vol. 2 No. 2 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.798 KB) | DOI: 10.20473/bhsj.v2i2.15410

Abstract

Introduction: Preterm and fullterm breast milk has different leukocyte and bioactive concentration, which affect it's bactericidal activity and storage duration. The preterm breast milk potentially has longer storage duration than fullterm breast milk The research and guideline about the acceptable of storage duration of preterm breast milk at 4o C was limited. The objective of this study was to assess the correlation of storage duration of preterm breast milk preterm baby at 4o C temperature on bacterial growth.Methods: The study design was experimental using pre-post control groups design. We used 9 samples of breast milk which met the inclusion criteria divided into 3 groups. The first, second and third groups was storage in 4o C for 0, 24 and 48 hours respectively before the inoculation to the culture media.Results: After the samples were kept at 4o C temperature for 0, 24 and 48 hours, the mean bacterial colony forming unit for each group were 92 (±SD 112), 79 (±SD 97) and 63 (±SD 73) CFU respectively. Compared to the first group, the second and third group has lower bacterial colony forming unit but no significant differences statistically (P= 0.214 and P=1.39 respectively). Likewise there was no significant bacterial colony forming unit between second and third group (P=0.086).Conclusion:  Storage of preterm breast milk in 4o C for 24 and 48 hours show no change in total bacterial colony count (TBCC)..
Antibacterial Effects of Basil (Ocimum sanctum) Leaf Extract in Combination with Meropenem against Extended Spectrum Beta-Lactamase (ESBL) Producing Klebsiella pneumoniae Lifindra, Billy Yanssen; Wiqoyah, Nurul; Indiastuti, Danti Nur
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 2 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I22024.88-93

Abstract

Highlights: Ocimum sanctum leaf extract does not significantly increase its antibacterial effects when combined with meropenem against ESBL-producing K. pneumoniae. Ocimum sanctum leaf extract has different antibacterial effects depending on geological and environmental factors.   Abstract Introduction: Antibiotic usage for bacterial infections causes antibiotic resistance in bacteria. One is extended-spectrum beta-lactamase (ESBL), which produces Klebsiella pneumoniae, a pathogen responsible for increased antibiotic resistance. Basil (Ocimum sanctum) is a candidate for combination therapy. It has been proven to have antibacterial effects. However, its combination with antibiotics is rarely researched. This study evaluated the antibacterial effects of O. sanctum leaf extract and meropenem combination against ESBL-producing K. pneumoniae. Methods: This study used the disk diffusion method. The extract was tested for each experiment at 6%, 4%, 2%, 1%, and 0.5% concentration. The research was divided into two experiments to evaluate the antibacterial effects of O. sanctum leaf extract (n=18) and its combination with meropenem against ESBL-producing K. pneumoniae (n=18). The data was analyzed using the International Business Machines Corporation (IBM) Statistical Package for the Social Sciences (SPSS) version 26.0 for Windows. A probability p<0.050 was considered significant. Results: The first experiment showed that the extract had the largest antibacterial effect at 0.5% concentration (n=18, p=0.007). Meanwhile, the second experiment showed that the combination of the extract and meropenem did not have significant antibacterial effects (n=18, p=0.597). Conclusion: Ocimum sanctum leaf extract has viable antibacterial effects, but its combination with Meropenem does not significantly improve its antibacterial effects against Extended Spectrum Beta-Lactamase (ESBL) producing K. pneumoniae.
Pemberdayaan Guru Yayasan Pendidikan Al Khairaat Yogyakarta pada Penanggulangan Antimicrobial Resistance: dari UNAIR untuk Indonesia Wahyunitisari, Manik Retno; Koendhori, Eko Budi; Endraswari, Pepy Dwi; Purwono, Priyo Budi; Juniastuti, Juniastuti; Wiqoyah, Nurul; Sardjono, Lindawati Alim; Vermasari, Naritha; Muna, Nafdzu Makhmudatul; Setiawan, Firman; Lindarto, Wira Widjaya; Widyatama, Fikri Sasongko
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 9 No 3 (2024): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v9i3.5481

Abstract

Antibiotics are compounds created by microorganisms to live by destroying their competitors. Antibiotics are commonly used without a prescription to treat symptoms caused by non-bacterial infections. Antibiotic resistance is a global problem. Continuous education is needed by agents of change to overcome this problem. Community service collaboration between the Department of Microbiology-Parasitology, FK Unair, and the Department of Microbiology, Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University with partnerships for the goals teachers from the Al Khairaat Yogyakarta educational foundation, aim to provide an understanding of the proper use of antibiotics. The initial target of transfer of knowledge was successfully measured by increasing participants' post-test results.
Co-Authors Agnes Dwi Sis Perwitasari, Agnes Dwi Sis Arifa Mustika Atika Bhagaskara, Pandya Deby Kusumaningrum Eko Budi Koendhori, Eko Budi Evy Ervianti Fanny Gunawan Indiastuti, Danti Nur Juniastuti Juniastuti Lifindra, Billy Yanssen Lindarto, Wira Widjaya Manaf, Syifa Az Zahrah Muhammad Ansori Muna, Nafdzu Makhmudatul Ni Made Mertaniasih Nur Rochmah, Nur Pepy Dwi Endraswari, Pepy Dwi Priyo Budi Purwono Purwanta, Marijam S. Soedarsono Salwa Putri Qurrotuaini Saputra, Pandit Bagus Tri Sardjono, Lindawati Alim Sekar Maharani Setiawan, Firman Setyarini, Wahyu Suhintam Pusarawati Ulfa Dwi Karisma Vermasari, Naritha Wahyunitisari, Manik Retno Wardatun Faizah Wayan Tunas Artama Widyatama, Fikri Sasongko Wilda Mahdani Willy Sandhika Wiwin Retnowati Yuani Setiawati