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Polymorphism of Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Genes among Pregnant Women with Falciparum Malaria in Banjar District, South Kalimantan Province, Indonesia Fitriah, Fitriah; Sulistyawati, Sriwijayanti; Riyanto, Sugeng; Budiono, Budiono; Basuki, Sukmawati; Dachlan, Yoes P; Uemura, Haruki
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pregnant women are highly vulnerable to malaria infection in its endemic areas, particularly infection by Plasmodium falciparum that can cause premature, low birth weight, severe anemia in pregnant women, and death. Sulfadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment for pregnant (IPTp) is used for malaria control in pregnancy recommended by the World Health Organization that has already been implemented in Africa. The P. falciparum resistance to SP has been reported in several malarial endemic areas, and mutations in the genes of Plasmodium falciparum Dihydrofolate Reductase (Pfdhfr) and Dihydropteroate Synthase (Pfdhps) are shown to be associated with parasite resistance to SP treatment. Genetic analysis of Pfdhfr and Pfdhps genes in pregnant women infected with P. falciparum has not yet been examined in Indonesia. The cross-sectional study was conducted at two subdistricts, Sungai Pinang and Peramasan, in Banjar district of South Kalimantan Province, where 127 pregnant women were recruited from 2008 to April 2010. Two important mutations in Pfdhfr gene (amino acid positions at N51 and S108) and three in Pfdhps gene (A437, K540 and A581) were analyzed by nested PCR-RFLP method. All of the seven pregnant women samples infected with P. falciparum presented PfDHFR 108N and PfDHPS 437G mutations. One of the samples had the additional mutation at PfDHPS 540, in which Lys is substituted by Glu. These results suggested that P. falciparum might present only some resistance to SP at Sungai Pinang and Peramasan subdistricts, Banjar District, South Kalimantan province, Indonesia. Although there were limited number of samples, this study showed only few mutations of Pfdhfr and Pfdhps genes in P. falciparum at Banjar district, South Kalimantan Province, that suggests SP might be effective for IPTp in this area. Thus, further analysis of the other mutation sites in Pfdhfr and Pfdhps genes and in vivo efficacy study of SP with more sufficient sample numbers will be necessary to confirm this preliminarily result.Keywords: PfDHFR and PfDHPS genes, Pregnant women, Falciparum malaria

Ekspresi mRNA Interleukin-10 (IL-10) dalam Kaitannya dengan Patogenesis Malaria Berat Pada Mencit Strain BALB/C yang Diinfeksi Plasmodium yoelli 17XL Sri Wijayanti Sulistyawati; Sukmawati Basuki; Yoes Prijatna Dachlan
Jurnal Biosains Pascasarjana Vol. 20 No. 3 (2018): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

AbstrakPatogenesis malaria berat masih belum diketahui menyeluruh. Episode malaria berat dapat disebabkan oleh dua keadaan, produksi tinggi pada fase awal IL-10 dan atau kurangnya produksi IL-10 pada fase transisi. Peran IL-10 pada malaria masih belum jelas diketahui. Penelitian ini bertujuan untuk menganalisis ekspresi IL-10 dalam kaitannya dengan patogenesis malaria berat pada mencit strain BALB/c yang diinfeksi P. yoelii 17XL.Penelitian eksperimental laboratoris dengan rancangan “post test only control group design” dilakukan, dan menggunakan 24 mencit strain BALB/c, betina, usia 7-8 minggu, yang dibagi menjadi 4 kelompok. Mencit BALB/c diinfeksi secara intraperitoneal dengan 1x105 P. yoelii 17XL, dan dikorbankan pada hari ke-3 dan ke-6 pasca infeksi. Parasitemia dan kadar hemoglobin diperiksa setiap hari. Jaringan limpa diambil untuk isolasi RNA. Ekspresi mRNA IL-10, TNFα, dan IFNγ dianalisis dengan RT-PCR. Mencit BALB/c dinfeksi dengan 1x105 P. yoelii 17XL menunjukan infeksi letal, yang ditandai dengan peningkatan parasitemia sejalan dengan penurunan kadar hemoglobin, terjadi setelah hari ke-3 pasca infeksi. Ekspresi mRNA IL-10, begitu pula dengan TNFα dan IFNγ pada hari ke-3 paska infeksi menunjukan peningkatan dibandingkan pada kontrol dan hari ke-6 paska infeksi. Tidak adanya ekspresi IL-10 pada H6, menunjukkan kemungkinan adanya kegagalan regulator mengontrol malaria berat pada infeksi P. yoelii 17XL pada mencit BALB/c. Kata kunci— mRNA IL-10, RT-PCR, patogenesis malaria berat, mencit BALB/c yang diinfeksi P. yoelii 17XL

Intestinal Protozoa Infections in Relation to Nutritional Status of the Students Mandangin Island Elementary School 6 in Sampang Regency Marlinda Kurniati; Budiono Budiono; Sri Wijayanti Sulistyawati
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 10 No. 1 (2019): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Introduction: Intestinal protozoa infections is a tropical infectious disease that can be found mainly in developing countries. It will cause some health problem such as undernutrition. This study was aiming to determine the correlation between the incidence of intestinal protozoa infections with the nutritional status of students Mandangin Island Elementary School 6 in Sampang Regency.Methods: This research used the cross-sectional study as the design and performed by doing a lab examination to determine the incidence of protozoa infections and analyze whether it has any correlation with changes in host nutritional status, recruited from students Mandangin Island Elementary School 6, from 19th to 21st of July 2017. Results: On microscopic examination found that 71.4% (n=30) samples are positive and 28,6% (n=12) are negative. On BMI-for-age measurement there are 4,8% obesity, 7,1% overweight, 73,8% normal, 9,5% thinness, and 4,8% severe thinness. Meanwhile, on height-for-age measurement there are 73,8% normal and 26,2% stunted . The incidence of intestinal protozoa infections was correlated with the nutritional status using SPSS (p= 0,375, p= 0,539, a= 5%).Conclusion: There was no significant correlation between intestinal protozoa infections with the nutritional status of students Mandangin Island Elementary School 6 in Sampang Regency. These conditions caused by the pathogenicity stage to cause a decrease in the nutritional state has not been reached.

PEMBERDAYAAN KARANG TARUNA “BIMANTARA” DAN IBU-IBU PKK KALIKEPITING JAYA VI KELURAHAN PACAR KEMBANG, KECAMATAN TAMBAKSARI, SURABAYA DALAM MENCEGAH PENULARAN COVID-19 Heny Arwati; Sri Wijayanti Sulistyawati; Lynda Rossyanti
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 5 No. 1 (2021): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v5i1.2021.49-57

The community service activity was carried out with the partner of Karang Taruna Bimantara, as a youth organization located in kampung Kalikepiting Jaya VI, RT 5, RW 5, Kelurahan Pacarkembang, Kecamatan Tambaksari, Surabaya City. This activity was aimed at reviving this organization which has been inactive for a long time and empowering the youths in preventing the transmission of Covid-19. The activities offered were providing enlightenment about Covid-19, assistance in producing liquid soap, hand sanitizers and non-medical cloth masks which could be developed into productive economic businesses. The implementation of these activities also involved the organization of Family Welfare Education (Pendidikan Kesejahteraan Keluarga or PKK) which was an organization of mothers in this kampung. The enlightenment were included Covid-19 and diseases transmitted by mosquitoes, their prevention and transmission. By producing the items than can be used to prevent transmission of Covid-19 followed by obedience to the health protocols, they could participate in preventing the transmission of Covid-19. In order to increase their knowledge on productive economy, the enlightenment on productive economy, marketing of goods and financial management of business, the enlightenment was also given. Hopefully the activities which can be developed into a productive economy business can increase the income of this kampung. The enlightenment provided pre-test and post-test. Comparison of both tests resulted in an insignificant increased in knowledge on both topics. More participants and more questions are recommended to improve the more significant scores of tests. The sales of liquid soap and hand sanitizer resulted in profit can be used to reproduce. Furthermore, more assistance is needed to develop a business into a productive economy.abstrakKegiatan pengabdian masyarakat ini dilaksanakan bersama mitra Karang Taruna Bimantara yang berada di kampung Kalikepiting Jaya VI, RT 5, RW 5, Kelurahan Pacarkembang, Kecamatan Tambaksari, Kota Surabaya. Kegiatan ini bertujuan untuk menghidupkan kembali organisasi yang sudah lama tidak aktif ini dan memberdayakan para pemuda dalam rangka pencegahan penularan Covid-19. Kegiatan yang ditawarkan antara lain adalah penyuluhan tentang Covid-19, bantuan pembuatan sabun cair, hand sanitizer dan masker kain non medis yang dapat dikembangkan menjadi usaha ekonomi produktif. Pelaksanaan kegiatan ini juga melibatkan ibu-ibu yang tergabung dalam organisasi PKK (Pendidikan Kesejahteraan Keluarga) yang merupakan wadah ibu-ibu di kampung ini. Penyuluhan yang diberikan adalah mengenai Covid-19 dan penyakit yang ditularkan oleh nyamuk, pencegahan dan penularannya. Dengan memproduksi barang-barang yang dapat digunakan untuk mencegah penularan Covid-19 diikuti dengan ketaatan terhadap protokol kesehatan, mereka dapat berpartisipasi dalam mencegah penularan Covid-19. Untuk menambah pengetahuan mengenai ekonomi produktif juga diberikan penyuluhan mengenai ekonomi produktif, pemasaran barang dan cara pengelolaan keuangan hasil usaha. Diharapkan dengan adanya kegiatan yang dapat dikembangkan menjadi usaha ekonomi produktif ini dapat meningkatkan pendapatan RT setempat. Dalam penyuluhan diberikan pre-test dan post-test. Perbandingan kedua tes menghasilkan peningkatan pengetahuan yang tidak signifikan pada kedua topik tersebut. Untuk meningkatkan nilai tes yang lebih signifikan, maka diperlukan jumlah peserta dan pertanyaan yang lebih banyak. Penjualan sabun cair dan hand sanitizer menghasilkan keuntungan yang dapat dimanfaatkan untuk memproduksi lagi. Pendampingan dibutuhkan untuk mengembangkan usaha ini menjadi ekonomi produktif.

EDUCATION PROGRAM AND FACILITY IMPROVEMENT FOR PERSONAL HYGIENE AT PONDOK PESANTREN METAL MUSLIM, PASURUAN Sri Wijayanti Sulistyawati; Dwi Peni Kartikasari; Lynda Rossyanti; Heny Arwati; M. Yasin Fitri Nugroho; Frista Amalia; Bagus Dwi Nugraha; Awwaliyah Azmi Izzati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 6 No. 1 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i1.2022.91-98

This community service activity was carried out with the partner of the Muslim Metal Islamic Boarding School, Pasuruan. This activity aims to improve the habits of clean and healthy living behavior (personal hygiene. The activities offered include examination of intestinal parasitic infections for residents of the Muslim Metal Islamic Boarding School, counseling on intestinal parasitic infections and personal hygiene and improvement of PHBS facilities. The counselling given is about various kinds of parasites that cause intestinal infections, transmission of intestinal parasitic infections, and how to prevent intestinal parasitic infections by implementing personal hygiene (PHBS). By knowing about intestinal parasites and how they are transmitted, be expected that they will be able to avoid infection with intestinal parasites by practicing PHBS discipline.

Profil Indeks Larva pada Delapan Wilayah Puskesmas di Surabaya LYNDA ROSSYANTI; DWI SUSANTI; IVAN RAHMATULLAH; DWI PENI KARTIKASARI; SUKMAWATI BASUKI; HENY ARWATI; INDAH TANTULAR; SUHINTAM PUSARAWATI; HERU PRASETYO; SRI HIDAJATI; SUBAGYO YOTOPRANOTO; SRI WIJAYANTI SULISTYAWATI
HANG TUAH MEDICAL JOURNAL Vol 17 No 2 (2020): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

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ABSTRACT Background : Dengue virus infection is still a health problem in Surabaya. On 2015 Insidence Rate (IR) for Surabaya is 22.50 and the Case Fatality Rate (CFR) is 2%. This figure has decreased compared to 2014, but has not met the target achievement indicator for the P2DBD target CFR <1%. Method : The study was conducted in the rainy season, May 2015 in several Puskesmas areas in Surabaya. Samples were randomly selected as many as 378 houses spread in eight health centers (Pegirian, Krembangan Selatan, Hamlet Wall, Tambak Rejo, Putat Jaya, Simomulyo, Sawahan and Tanahkali Kedinding). The larvae survey was conducted using the single larva method. Larvae were taken from breeding sites and stored in specimen bottles for species identification at the Parasitology Laboratory Faculty of Medicine Universitas Airlangga. Result : The highest House Index (HI) was obtained (60.46%) or the 39.54% larvae free rate in Simomulyo and the lowest HI (33.33%) or 66.67% ABJ in Pegirian Puskesmas. Larvae species identification showed 100% was Aedes aegypti. The high HI value at the Simomulyo Health Center is not in line with the number of cases of dengue virus infection in 2015 which was 4 cases. Whereas in Pegirian Health Center with the lowest HI value found 11 cases. Discussion : These results could be due to the possibility because the study did not calculate larval density and high population mobility in the area.

Polymorphism of Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Genes among Pregnant Women with Falciparum Malaria in Banjar District, South Kalimantan Province, Indonesia Fitriah Fitriah; Sriwijayanti Sulistyawati; Sugeng Riyanto; Budiono Budiono; Sukmawati Basuki; Yoes P Dachlan; Haruki Uemura
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pregnant women are highly vulnerable to malaria infection in its endemic areas, particularly infection by Plasmodium falciparum that can cause premature, low birth weight, severe anemia in pregnant women, and death. Sulfadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment for pregnant (IPTp) is used for malaria control in pregnancy recommended by the World Health Organization that has already been implemented in Africa. The P. falciparum resistance to SP has been reported in several malarial endemic areas, and mutations in the genes of Plasmodium falciparum Dihydrofolate Reductase (Pfdhfr) and Dihydropteroate Synthase (Pfdhps) are shown to be associated with parasite resistance to SP treatment. Genetic analysis of Pfdhfr and Pfdhps genes in pregnant women infected with P. falciparum has not yet been examined in Indonesia. The cross-sectional study was conducted at two subdistricts, Sungai Pinang and Peramasan, in Banjar district of South Kalimantan Province, where 127 pregnant women were recruited from 2008 to April 2010. Two important mutations in Pfdhfr gene (amino acid positions at N51 and S108) and three in Pfdhps gene (A437, K540 and A581) were analyzed by nested PCR-RFLP method. All of the seven pregnant women samples infected with P. falciparum presented PfDHFR 108N and PfDHPS 437G mutations. One of the samples had the additional mutation at PfDHPS 540, in which Lys is substituted by Glu. These results suggested that P. falciparum might present only some resistance to SP at Sungai Pinang and Peramasan subdistricts, Banjar District, South Kalimantan province, Indonesia. Although there were limited number of samples, this study showed only few mutations of Pfdhfr and Pfdhps genes in P. falciparum at Banjar district, South Kalimantan Province, that suggests SP might be effective for IPTp in this area. Thus, further analysis of the other mutation sites in Pfdhfr and Pfdhps genes and in vivo efficacy study of SP with more sufficient sample numbers will be necessary to confirm this preliminarily result.Keywords: PfDHFR and PfDHPS genes, Pregnant women, Falciparum malaria

Intestinal Parasitic Infection, The Use of Latrine, and Clean Water Source In Elementary School Children At Coastal And Non-Coastal Areas, Sumenep District, Indonesia Renaldy, Raden Bagus Yanuar; Aflahudin, M. Ahda Naufal; Salma, Zukhaila; Sumaryono, Sumaryono; Fitriah, Muhammad Yasin; Sulistyawati, Sri Wijayanti; Husada, Dominicus; Basuki, Sukmawati
Indonesian Journal of Tropical and Infectious Disease Vol. 9 No. 1 (2021)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v9i1.22578

Inadequate latrine and water source cause transmission of intestinal parasitic infection, particularly in children. There is a lack information about it and it is needed to be investigated. This study aimed to compare the prevalence of intestinal parasitic infection, the use of latrine and clean water source in elementary school children at coastal and non-coastal areas in Sumenep District, Indonesia. An analytic observational study with cross sectional design was conducted in Dasuk Timur Elementary School located at coastal area, and Kolor II Elementary School at non-coastal area, Sumenep district, in January 2020. Intestinal parasites in students' stools were identified by microscopic examination using wet direct smear stained with lugol. The use of latrine and water sources were analyzed with questionnaire. A total of 68 children stools were collected from both elementary schools. Worm infections were not found. Thirty-one children (31/44, 70.5%) from Dasuk Timur Elementary School and eight children (8/24, 33.3%) from Kolor II Elementary School were infected with intestinal protozoan and significant difference (P=0.003, Chi-square test). Blastocystis hominis was highly found in stools of Dasuk Timur Elementary School's students (31/44, 70.5%) and significantly different from Kolor II Elementary School's students (P<0.0001, Chi-square test). Three children (3/44, 6.8%) from Dasuk Timur Elementary School were still practicing open defecation. Dasuk Timur Elementary School's students suffered from intestinal parasitic infection were mostly using non-piped water source (20/31, 64.5%) and were significantly different between two elementary schools (P=0.015, Fisher's exact test). Prevalence of intestinal parasitic infections in children was found higher in coastal than non-coastal area due to the commonly use of unclean water sources and inadequate latrine.

The Longevity of Aedes aegypti Larvae in Several Water Sources in Surabaya Antonio Ayrton Widiastara; Gabriel Pedro Mudjianto; Etik Ainun Rohmah; Hengki Anggara Putra; Martha Indah Widia Ningtyas; Sri Wijayanti Sulistyawati; Suhintam Pusarawati; Fitriah; Kasiyama Desi Indriyani; Alpha Fardah Athiyyah; Basuki, Sukmawati
Indonesian Journal of Tropical and Infectious Disease Vol. 10 No. 1 (2022)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v10i1.32209

Aedes aegypti transmits the dengue virus that causes Dengue Viring the high number of DVI cases is the existing breeding places of Ae. aegypti. The water sources used by the community and the surrounding environment are essential media for living Ae. aegypti larvae. This recent study aimed to detect the longevity of Ae. aegypti larvae in diï¬€ erent water sources in Surabaya and the killing eï¬€ect of temephos. An analytical observational and experimental study was conducted in August-September 2021. Twenty-instar III Ae. aegypti larvae were put in each 100 ml beaker glass containing diï¬€ erent water sources, such as rain, well, mineral, new and used bath water, and antiseptic soapy water. Fungi in water sources were examined. Two groups were set with and without temephos, the ï¬nal temephos concentration was of 0.00001 ppm. Live Ae. aegypti larvae, pupae, mosquitoes were observed every 24 hours for seven days without feeding. Living larvae were still found on Day 7 in all water sources with and without temephos. There were more larvae live in soapy water without temephos, particularly on Day 2 to Day 6, compared to other water sources either without or with temephos. In contrast, many larvae died in mineral water with temephos. Some larvae turned into pupae, started on Day 1. Pupae and mosquitoes were mostly found in rain water with temephos. Ae. aegypti larvae survived better in soapy water either with or without temephos. Temephos seemed to be eï¬€ective to kill Ae. aegypti larvae in mineral water, and might induce larvae in turning to pupae and mosquitoes quickly at low concentration.

Parent's Level of Stress on Children's Online Classes during the COVID-19Pandemic Nurrosyida, Kartika; Firmanto, Neissya Nastiti; Fayanshah, Irda Bella Ajeng; Sulistyawati, Sri Wijayanti
Journal of Community Medicine and Public Health Research Vol. 3 No. 1 (2022): Journal of Community Medicine and Public Health Research
Publisher : Universitas Airlangga

The Covid-19 pandemic is affecting people's lives. One of the sectors hampered by the pandemic is education. During the Coronavirus Disease (Covid-19) Emergency, the teaching and learning process must be replaced by online methods. The role of mothers is very important in ensuring that children's learning targets are achieved during distance learning. This can cause stress conditions for the mother. This study aimed to determine the perceptions and various problems that occurred in mothers with children aged 7-15 years old who attended online schools during the Covid-19 pandemic. This was a descriptive study by distributing questionnaires via google form with 67 respondents. Based on the results, the majority of respondents were 35-45 years old (49.3%). Over 85.6% of respondents accompanied their children in online learning activities during the pandemic. The majority of respondents experienced an increase in emotions (70.1%) and felt stressful when accompanying online school children during the pandemic (62.7%). As many as 40% of respondents felt a moderate level of stress. There were 27 mothers (40%) who had difficulty assisting their children in online schooling and 24 mothers (36%) felt that their professional job was abandoned while their children were having online school.

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