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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Sumberdaya Hayati Jurnal Medik Veteriner
Susan Maphilindawati Noor
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Journal : Jurnal Ilmu Ternak Veteriner

 1 
The mucosal and systemic immune responses in chickens orally immunised with Campylobacter jejuni antigen entrapped in poly-lactide-co-glycolide microparticles Noor, Susan Maphilindawati
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.249 KB) | DOI: 10.14334/jitv.v3i4.127

Abstract

An oral immunisation in chickens with antigen entrapping in biodegradable microparticles was evaluated in order to achieve optimal antibody responses following oral vaccination. This study was adapted to Campylobacter jejuni antigen in chickens to observe its stimulation both mucosal and systemic immune responses. A group of 5 embryonated chicken eggs was immunised with heat-killed C. jejuni entrapped in poly-lactide-co-glycolide (PLG) microparticles at day 17 of incubation deposited into the amniotic fluid. Seven days after hatching the chickens were oral boostered, these was design to as Group A. In the Group B, each embryonated egg was immunised with soluble C. jejuni as in the same as Group A. Immune responses of post vaccination were observed at day-14, the humoral immunity was evaluated with an ELISA and whereas mucosal antibody response was detected by fluorescent histology. The serum IgG and IgA antibody responses, and also the bile and intestinal scrapping IgA antibody responses to campylobacter in Group A were significantly higher than those of the soluble antigen of Group B (P<0.05). Total number of immunoglobulin-containing cells for IgG, IgM, and IgA isotypes in the ileum in Group A chickens were also significantly higher than those of Group B (P<0.05) but was not in the duodenum and spleen. Key words : Campylobacter jejuni, poly-lactide-co-glycolide microparticles, oral immunisation, chickens
Study in ovo immunisation with flagellin and whole cell protein antigens of Campylobacter jejuni in chickens Noor, Susan Maphilindawati; Husband, Alan J
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 2 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.048 KB) | DOI: 10.14334/jitv.v5i2.208

Abstract

In ovo immunisation of chickens with flagellin and whole cell protein antigens of Campylobacter jejuni was examined to determine Campylobacter infection. Four groups of embryonated chicken eggs (10 eggs per group) were immunised in ovo at day 17 of incubation and booster was given at 7 days post-hatch. Group I was immunised in ovo and oral booster with whole cell protein of C. jejuni, group II was immunised in ovo and oral booster with C. jejuni flagellin protein, group III was immunised in ovo and intraperitoneal booster with whole cell, and group IV was treated as control. The humoral immune responses were determined by enzyme-linked immunosorbent assay (ELISA) and the mucosal immune responses were examined by a direct fluorescent histology antibody technique. Immunised chickens of Group I, II, and III shown to have higher antibody titers than  those of control chickens (group IV). The titres of anti-campylobacter antibodies of all isotypes in serum, bile, and intestinal scrapping after challenge were not significantly different in all groups. In addition, when immunised chickens were orally challenged with a homologous strain of viable C. jejuni organism, the chickens remained infected throughout the experiment based on cloacal swabs and caecal contents. These findings indicated that although in ovo immunisation resulted in increasing of the mucosal and humoral immune responses in chickens, it is not strong enough to protect the Campylobacter colonisation in the intestinal tract.   Key words : In ovo immunisation, Campylobacter jejuni, whole cell, flagellar, chickens
Co-Authors Alan J Husband Firdaus Ramadhan, Firdaus Fitrine Ekawasti, Fitrine I wayan Teguh Wibawan Irianti, Ewith Ratih Khairullah, Aswin Rafif Puarada, Alif Rahman Rohim Purba, Hastuti Handayani S. Ramadhani, Lidia Anggita Safika S, Safika Sri Suryatmiati Prihandani