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Antibacterial of Cinnamon Bark (Cinnamomum burmannii) Essential Oil Against Methicillin-Resistant Staphylococcus aureus Fadlilah, Shinta Levea Ni'matul; Effendi, Mustofa Helmi; Tyasningsih, Wiwiek; Suwanti, Lucia Tri; Rahmahani, Jola; Harijani, Nenny; Ramandinianto, Sancaka Chasyer; Khairullah, Aswin Rafif
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.56-62

Abstract

This study aimed to determine the in vitro antibacterial activities of essential oil from cinnamon bark (Cinnamomum burmannii) on Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk. Essential oil from cinnamon bark obtained from the steam distillation method and essential oil was made in a series dilution with a concentration of 1%, 2%, 4%, and 8%. The antibacterial activities were tested using the disk diffusion method. Results showed from five isolates of MRSA, one isolate was sensitive of essential oil with a concentration 2% and all of the isolate was sensitive with a concentration 4% and 8%. The antibacterial activity showed by inhibition zones on  MRSA. The results suggest that the activity of the essential oils of cinnamon bark has an antibacterial effect on MRSA and it is developed as phytopharmaca.
Serological Study of Newcastle Disease in Ducks (Anas javanicus) Slaughtered in East Surabaya Traditional Market Ardhanella, Sellianova; Damayanti, Ratna; Suwarno, Suwarno; Rantam, Fedik Abdul; Rachmawati, Kadek; Khairullah, Aswin Rafif; Rahmahani, Jola
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.131-137

Abstract

The purpose of this study was to determine the serological study of Newcastle Disease that infects slaughtered ducks (Anas javanicus) at the East Surabaya Traditional Market. This study was conducted from December 2018 to January 2019. A total of 122 samples were collected from four traditional markets in East Surabaya. The Haemagglutination Inhibition (HI) test was used to determine a positive result with an antibody titer of 4 or more. Samples for the HI test were collected from duck serum that had been treated with chicken red blood cells. The results showed 13 (10.65%) of the 122 samples were positive for ND.
Identification of Escherichia coli Multidrug Resistance in Cattle in Abattoirs Mustika, Yolla Rona; Effendi, Mustofa Helmi; Puspitasari, Yulianna; Plumeriastuti, Hani; Khairullah, Aswin Rafif; Kinasih, Kurnia Nisa
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.19-32

Abstract

Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this study was done on the detection and identification of multidrug-resistant E. coli in cattle in Surabaya abattoirs. Each cattle rectum swab sample was streaked into EMB agar media and followed by Gram staining. The IMViC test was used to confirm the presence of E. coli. Based on morphological culture features, Gram staining, and biochemical testing, the sample examination results revealed that 41 samples (41%) of the 100 cattle rectal swab samples that were isolated were confirmed to be positive for E. coli. The profile of antibiotic resistance from the results of the E. coli resistance test to antibiotics showed that out of a total of 41 isolates of E. coli, 12 isolates (29.27%) were detected as resistant to 1 class of antibiotics tested, while 2 isolates (4.88%) were resistant to 2 classes of antibiotics, and 3 isolates (7.32%) were confirmed to be multidrug resistance (MDR).
Diluent and Storage Time Effect on Sperm Abnormality and MDA Level in Muscovy Duck Semen at 27oC Andaruisworo, Sapta; Tanjungsari, Ardina; Yuniati, Erna; Khairullah, Aswin Rafif
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.79-90

Abstract

This study aimed to determine sperm abnormality and MDA level in Muscovy duck semen in different dilution and storage at 27oC. This study was used in the level of dilution of glutinous semen with a long time store differently at 27oC. In this study, the parameters of the mass motility of spermatozoa were used individual motility, spermatozoa abnormalities, and activity test. A Muscovy duck, healthy and have a high libido were used then divided into treatment groups i.e. (A0) 0; (A1) 5 times; (A2) 10 times; and (A3) 15 times, the second factor as a subplot was the storage time (BO) 0; (B1) 60 minutes; (B2) 120 minutes; and (B3) 180 minutes stored at 27oC with 3 replications. In results, this study was reported abnormalities at a dilution rate in A1 group of 5 times with a shelf life in B1 group of 60 minutes and in B2 group of 120 minutes.
Efficacy of Sauropus androgynous Leaves Extract Gel on Burn Wound Healing in Albino Rats Panggabean, Rosenna Tiara; Sudjarwo, Sri Agus; Ma'ruf, Anwar; Widiyatno, Thomas Valentinus; Yudaniayanti, Ira Sari; Kurnijasanti, Rochmah; Khairullah, Aswin Rafif
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.107-115

Abstract

Burns not only damage skin locally but generally affect the body system and have been related as the secondary cause of death. Burns can be effectively treated with a topical drug to prevent chronic inflammation. Burn wound healing is really important for ensuring overall health and well-being. This study aimed to determine the effect of Sauropus androgynous leaves extract gel on the number of fibroblasts, fibrocytes, and collagen density on burn wound healing in albino rats. A total of 50 male albino rats were randomly divided into five groups i.e. (C-) was normal skin, (C+) was skin burn treated with placebo, (T1) was skin burn treated with 2.5% of S. androgynous leaves extract gel, (T2) was skin burn treated with 5% of S. androgynous leaves extract gel, and (T3) was skin burn treated with 10% of S. androgynous leaves extract the gel. The amount of 25 albino rats' skin samples were collected on the 8th day, and the remaining samples were collected on the 15th day. The results showed that 10% of S. androgynous leaves extract gel improved faster, as indicated by the increased number of fibroblasts, fibrocytes, and collagen density. This study can be concluded that 10% of S. androgynous leaves extract gel was the effective dosage to increase the number of fibroblasts, fibrocytes, and collagen density in burn wound healing.
Effectiveness of Phyllanthus niruri and Andrographis paniculata Extracts on Egg Quality in Laying Hens with Avian Pathogenic Escherichia coli Hidanah, Sri; Sabdoningrum, Emy Koestanti; Chusniati, Sri; Nurliyani, Nurliyani; Khairullah, Aswin Rafif; Nayan, Nazri
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.48-54

Abstract

Providing sufficient and high-quality eggs plays an essential role in accommodating food safety and food security for consumers. The purpose of this study was to determine the effect of Phyllanthus niruri and Andrographis paniculata extracts on egg yolk index and haugh units (HU) value in laying hens with Avian Pathogenic Escherichia coli. This type of study was experimental using a complete randomized design with 100 experimental units of five treatments (P-) placebo, (P+) infected with Avian Pathogenic E. coli without P. niruri and A. paniculata extract, then groups of chickens infected with Avian Pathogenic E. coli and administration of (P1) 10% of P. niruri and 30% of A. paniculata, (P2) 20% of P. niruri and 20% of A. paniculata, (P3) 30% of P. niruri and 10% of A. paniculata then egg yolk index and HU value were evaluated. The data obtained were analyzed using ANOVA followed by Duncan's test. The results showed a significant difference between the treatment groups based on the egg yolk index and the HU value. It can be concluded that P2 can be recommended for laying hens with avian pathogenic E. coli.
Detection of the CTX-M Gene Associated with Extended-Spectrum β-Lactamase (ESBL) in Broiler Chickens in Surabaya Traditional Markets Putri, Mariana Febrilianti Resilinda; Khairullah, Aswin Rafif; Effendi, Mustofa Helmi; Wibisono, Freshinta Jellia; Hasib, Abdullah; Moses, Ikechukwu Benjamin; Fauziah, Ima; Kusala, Muhammad Khaliim Jati; Raissa, Ricadonna; Yanestria, Sheila Marty
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.320-334

Abstract

A common indicator used to examine the frequency and distribution of antibiotic resistance against other enteric bacteria in humans and animals is the commensal enteric bacterium, Escherichia coli. The transmission of plasmids harboring ESBL enzymes, primarily generated by E. coli, is the cause of this resistance. The purpose of this study was to identify the CTX-M gene in ESBL-producing E. coli from broiler chicken cloacal swabs in traditional Surabaya markets. The samples used were 96 cloacal swabs from broiler chickens in the traditional markets of Dukuh Kupang, Keputran, Pacar Keling, and Pucang. The antibiotic disks used in this study belonged to five different antibiotic classes; they are aztreonam (monobactam), chloramphenicol (phenicol), kanamycin (aminoglycoside), ciprofloxacin (fluoroquinolone), and tetracycline (tetracycline). Presumptive ESBL strains were then molecularly screened for the presence of CTX-M gene. Results revealed that out of the 96 chicken cloacal swab samples collected, 58 (60.42%) were positive for E. coli based on morphological culture, Gram staining, and biochemical tests. Additionally, 15 out of the 58 E. coli isolates recovered from broiler chicken cloacal swabs were multidrug-resistant (MDR) while 7 of E. coli isolates harbored CTX-M gene. Conclusively, this study has shown that broiler chickens sold in traditional Surabaya markets harbor MDR E. coli which possess CTX-M gene. Conditions in traditional markets with low levels of cleanliness and chickens placed close together can spread resistance genes with serious public health consequences. Therefore, it is imperative to observe good hygienic practices in Surabaya traditional markets in order to curtail the spread of MDR bacterial pathogens in the food chain.
Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Sidoarjo, Indonesia Sari, Aulia Puspita; Koesdarto, Setiawan; Lestari, Tita Damayanti; Hastutiek, Poedji; Yunus, Muchammad; Herupradoto, Eduardus Bimo Aksono; Khairullah, Aswin Rafif; Ayuti, Siti Rani; Hasib, Abdullah; Aryaloka, Suhita; Moses, Ikechukwu Benjamin
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.370-381

Abstract

The problem most often encountered in fish farming is disease which can cause a decrease in fish production levels. Parasites cause one of the infectious diseases found in fish farming. The presence of parasites can cause a decrease in the quality of the growth of fish production. The purpose of this study was to identify the prevalence of ectoparasites on Nile tilapia in Sidoarjo, Indonesia. Ninety-six samples from two ponds were examined under the microscope to find out the presence of ectoparasite that exists in the outside body part of the fish. Samples were collected from skin, fins, tail, and gills using native methods and observed under a microscope in the Division of Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. The results of this study showed 86 samples positively infected by ectoparasite and 10 showed negative. The ectoparasites found in this study were Trichodina sp., Chilodonella sp., Ichthyophthirius multifillis, Gyrodactylus sp., and Dactylogyrus sp. The highest prevalence is Trichodina sp. and Dactylogyrus sp. The number of fish infested by ectoparasite is 89.56%, this is classified in the usual category. The highest prevalence occurs in infestations of Trichodina sp. i.e.72.91% which were contained in the 70 samples. While the lowest prevalence was in Gyrodactylus sp. by 8.32% in 8 fish samples. Double infections from two different ponds were 70% within 33 samples which were included in the usual category.
Cellulase Enzyme Production Using Actinobacillus sp. on Several Alternative Growth Media Al-Arif , Mohammad Anam; Lamid, Mirni; Warsito, Sunaryo Hadi; Khairullah, Aswin Rafif; Hasib, Abdullah
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.221-231

Abstract

Cellulase enzymes are widely used in the food, beverage, animal feed, textile, and paper industries. The high cost of producing enzymes and low enzyme activity provides opportunities for using chemicals, and efforts are needed to produce cellulase enzymes economically through media optimization. This research aims to find alternative media that make it possible to produce cellulase enzymes with high activity. This research created a control media (M0) using pro-analysis chemicals and CMC as a carbon source. Three types of cellulolytic bacterial growth media were also created, consisting of technical chemicals, rice straw as a substrate, and different protein sources. M1: alternative media using urea as a protein source; M2: alternative media using powdered milk as a protein source; and M3: alternative media using chicken liver as a protein source. A total of 1% of cellulolytic bacteria (Actinobacillus sp.) was grown in each media and then incubated at 40°C for 33 hours at a speed of 140 rpm, and the production curve and cellulase enzyme activity were measured at every 3-hour interval. The results showed that the highest cellulase enzyme production was achieved at 24 hours using alternative media with powdered milk (M2) as a protein source. The cellulase enzyme activity produced was 2.9612 µ/ml.
The Correlation between the Weight of Ovary, Number of Follicles, and Quality of Oocytes of Culling Female Bovine in Slaughterhouse Chandramohan, Koshini; Utama, Suzanita; Lestari, Tita Damayanti; Rimayanti; Safitri, Erma; Hestianah, Eka Pramyrtha; Mulyati, Sri; Damayanti, Ratna; Khairullah, Aswin Rafif; Hasib, Abdullah
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.206-220

Abstract

The purpose of this research was to determine the correlation between the weight of the ovary, the number of follicles, and the quality of the oocytes from the culling female bovine   obtained from the slaughterhouse. The variation may affect the potential of the ovary to produce follicles. Paired ovaries were obtained from female bovines.  Follicle numbers were recorded and divided into three size categories (small: 3 mm, medium: < 3 mm-8 mm, and large: > 8 mm). Oocytes were aspirated and the number of oocytes was recorded and graded into four categories (grades A, B, C, and D). There was a positive correlation between the weight of the ovary and the number of follicles, which is 0.560 with the regression equation y = 3.52 + 0.501 x. There was a positive correlation between the number of follicles and the number of oocytes, which is 0.546 with the regression equation y =2.48 + 1.204 x. There was a positive correlation between the number of oocytes and the grade of oocytes, which is 0.520, with the regression equation y = 0.93 + 0.800 x. There was no correlation between the weight of the ovary and oocyte grade A, which is 0.013.
Co-Authors . Kholik Abuzahra, Mutasem Adi Sofyan Ansori, Muhammad Afnani, Daniah Ashri Agung Prasetyo Aini, Nur Sofiatul Akintunde, Adeyinka Oye Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Al Baariq, Hisyam Nabil Najmuddin Ali Agus Amrullah, Muhammad Fajar Ardhanella, Sellianova Aryaloka, Suhita Ayuti, Siti Rani Azis, Nurul Magfirah Bezhinar, Tatyana Brahmantya, Chandra Burkov, Pavel Chandramohan, Koshini Chusniati, Sri Derkho, Marina Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Erma Safitri Erna Yuniati Fachry Abda El Rahman Fadlilah, Shinta Levea Ni'matul Fauzia, Kartika Afrida Fauziah, Ima Fedik Abdul Rantam Fitrine Ekawasti, Fitrine Gudz, Petr Guselsa, Fricelia Aura Hani Plumeriastuti Harijani, Nenny Hasib, Abdullah Herdiansyah, Mochammad Aqilah Herry Agoes Hermadi Hery Purnobasuki Hidanah, Sri Hidayatik, Nanik I wayan Teguh Wibawan Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Ira Sari Yudaniayanti Kadek Rachmawati Karina, Soca Karjono Karmanis Khairunnisa, Rahma Kharisma, Viol Dhea Kinasih, Kurnia Nisa Kuncorojakti, Suryo Kurniasih, Dea Anita Ariani Kurniawan, Muhammad 'Ahdi Kurniawan, Shendy Canadya Kusala, Muhammad Khaliim Jati Kusnoto Kusnoto, Kusnoto Kusumawati, Evyana Diah Lucia Tri Suwanti, Lucia Tri Ma'ruf, Anwar Maksimiuk, Nikolai Mirni Lamid Moses, Ikechukwu Benjamin Mufasirin Munawir Sazali Murtadlo, Ahmad Affan Ali Mustika, Yolla Rona Mustofa Helmi Effendi Nayan, Nazri Nur Rusdiana Nurliyani Nurliyani Pamungkas, Fajrin Yudha Panggabean, Rosenna Tiara Pasang, Gabriel Sampe Poedji Hastutiek Pratama, Bima Putra Puarada, Alif Rahman Rohim Purba, Hastuti Handayani S. Putra, Oktavian Arya Putri, Mariana Febrilianti Resilinda Putri, Mirtanti Kiswandi Putri, Natalie Lovena Pramono Raharjo, Dadik Rahmahani, Jola Rahmawati, Septyana Eka Raissa, Ricadonna Ramadhan, Nuzula Rijal Nur Ramandinianto, Sancaka Chasyer Ratna Damayanti Rebezov, Maksim Rimayanti Rimayanti Rimayanti Riska Riska Riwu, Katty Hendriana Priscilia Riza Zainuddin Ahmad Rochmah Kurnijasanti Rollando, Rollando Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanti Safika S, Safika Santoso, Kuncoro Puguh Sapta Andaruisworo Sari, Aulia Puspita Setiawan Koesdarto Sheila Marty Yanestria Sri Mulyati Sri Suryatmiati Prihandani Suherni Susilowati Sukmanadi, Mohammad Supriyadi Supriyadi Susan Maphilindawati Noor Suwarno Suwarno Suzanita Utama Tanjungsari, Ardina Teguh Hari Sucipto, Teguh Hari Tika Hafzara Siregar Tita Damayanti Lestari Tyasningsih, Wiwiek Ulhaq, Oktania Dhiya Wahyuningtias , Dita Sheila Putri Wardhani, Bantari Wisynu Kusuma Warsito, Sunaryo Hadi Wibisono, Freshinta Jellia Wibowo, Syahputra Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Widyananda, Muhammad Hermawan Yulianna Puspitasari Yunus, Muchammad