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Lies Parede
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Journal : Jurnal Veteriner

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PHYLOGENETIC ANALYSIS OF NUCLEOTIDE SEQUENCE OF HIPERVARIABLE FRAGMENT OF VP2 OF INFECTIOUS BURSAL DISEASE VIRUS ISOLATED IN INDONESIA I Gusti Ngurah Kade Mahardika; Lies Parede
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Nucleotide sequence of hypervariable fragment of VP2 of infectious bursal disease virus (IBDV) isolated in Bali and Indonesia were compared to standard virulent (svIBDV’s) and very virulent (vvIBDV’s) viruses accessed in GeneBank. The comparation were done using the multiple sequence alignment program of Mega 3.1. The sequence were aligned by the ClustalW Method (Mega 3.1), that is grouping the sequences into clusters by examining the distance between all pairs. The sequences were aligned pair wise, then as groups. Phylogenetic tree were constructed using Neighbor-Joining and Bootstrap-tested. The analysis showed that IBDV can be clustered into 2 major groups, i.e. America-Europe and Australia clusters. The America-Europe cluster is further divided into two sub-clusters, i.e. classic IBD and vv-IBD. Classic-IBD is represented by standard STC, Cu-1, Variant A, F52-70, and PBG98 viruses. Two IBDV’s isolated in Bali and the most of other Indonesian isolates belong to sub-cluster vv-IBD and share a common cluster with IBDV that were recently reported as very virulent strains. One exceptional isolate is Indo 13, which is groupped into classic IBD, and closely related to classical American standard virus, STC.
Patogenesitas Virus Gumboro Isolat Lokal pada Ayam Pedaging Sutiastuti Wahyuwardani; Dewi Ratih Agungpriyono; Lies Parede; Wasmen Manalu
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Study of the pathogenicity of gumboro virus (very virulent Infectious Bursal Disease virus, vvIBDV) oflocal isolates was performed in broilers. The chickens were grouped into four: i) infected with vvIBDV; ii)vaccinated with commercial vaccine + challenge with vvIBDV; iii) vaccinated using locally produced vaccine+ challenged with vvIBDV; and iv) control group (unvaccinated animals). Pathogenecity was analyzedbased on the sequence of infection; the distribution and degree of gross pathology and histopathologylesions on bursa Fabricius, spleen and thymus in relation to the presence of the antigen (IBDV Ag) whichwas detected using immunohistochemistry technique. During the acute phase (1-3 days following theexperimental infection) hyperemia and exudation on the bursa Fabricius were observed in the infectedgroup and the two vaccinated-challenged groups. Similarly, hyperemia was also observed on the spleenand thymus. Whilst during the chronic stages (at 7 and 14 days following the experimental infection) thebursa showed atrophy. Upon histological examination, the bursa showed interstitial edema, hemorrhage,infiltration of heterophyl cells and fibroblast hyperplasia. The bursa lymphoid follicles were depleted dueto cells necrosis and apoptosis. Plica epithelial layer was wrinkled and the Goblet cells metaplasia formedhyper plastic cysts. In the spleen and thymus severe reticulo endothelial cells proliferation was observedduring the acute stage and tended to decline during the chronic stage. During the acute and chronic stagescells containing IBDV Ag were detected in the bursa Fabricius, distributed within the mantle zone andgerminal center of lymphoid follicles in the two vaccinated-challenge groups. Whereas in the infectedgroup, the IBDV antigen were detected up to 7 days post the infection. Nevertheless, neither IBDV antigenwas detected in the spleen nor in the thymus in all the experimental groups. The results of this studyclearly explained the ferocity of vvIBDV of local isolates.
Co-Authors Dewi Ratih Agungpriyono Hernomoadi Huminto I Gusti Ngurah Kade Mahardika Risa Indriani RISA INDRIANI Sukarsih . Sutiastuti Wahyuwardani Sutiastuti Wahyuwardani Wasmen Manalu