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All Journal Jurnal Akuakultur Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Torani Journal of Fisheries and Marine Science Aquacultura Indonesiana

Dody Dharmawan Trijuno, Dody Dharmawan

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Author-ID : 491377

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Social Sciences

Published : 7 Documents Claim Missing Document

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Articles

Acceleration of Blue Swimming Crab (Portunus pelagicus) Larval Development by Phytoecdysteroid Ahmad, Fakhirah; Fujaya, Yushinta; Trijuno, Dody Dharmawan; Aslamyah, Siti
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Phytoecdysteroid is a mimic hormone like molt regulating hormone in arthropoda and crustacea. This research aims to investigate the response of Portunus pelagicus larvae on various doses of vitomolt enrichment in predigested artificial diet. Four doses of vitomolt (0; 4; 40; 400 mg/100 g artificial diet) were used in this research. Larval development and survival rate were measured The results showed that the larvae development rate was increased in congruent with the dose of vitomolt. However, highest survival rate was found on 4 mg vitomolt/100 g artificial diet (P>0.05). Based on regression analysis, it is estimated the optimal dose of vitomolt for larval development rate and survival rate was about 23 mg/100 g of artificial diet. Vitomolt application in artificial diet could be used to accelerate the larval development rate in blue swimming crab. Keywords: Portunus pelagicus, phytoecdysteroid, artificial diet, survival, larval development

The Morphometric Character and Mitochondrial 16S rRNA Sequence of Portunus pelagicus Fujaya, Yushinta; Hidayani, Andi Aliah; Asphama, Andi Ivo; Trijuno, Dody Dharmawan; Tenriulo, Andi; Parenrengi, Andi
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Blue swimming crab (Portunus pelagicus) colors have variations from different habitats and they have different vitality. The purpose of this study was to analyze the diversity of the P. pelagicus using morphometric and 16SrRNA mitochondrial sequence. There were three groups of blue swimming crab characterized by differences in color and density patterns of spots. The result of Canonical discriminant analysis showed that there were morphometric differences among the three groups. The main distinguishing morphometric characteristics are the ratio of carapace width and length as well as the ratio of the length and width of cheliped merus. The third group of crab is also has similarity about 98-99% by the Portunus pelagicus 16S rRNA sequences available in gene banks. These studies had concluded that differences in color and pattern of the spot density have also morphometric and genetic differences. At least there were two groups based on genetic variation of Portunus pelagicus from Barru Regency waters which may have a variety of different properties. Further research is needed to study the adaptation abilities of the two groups of crab on the condition of aquaculture as a potential domestic animal.Keywords: Crab; Portunus pelagicus; Morphometric; 16S rRNA.

Quality of Blue swimming crab Portunus pelagicus Larvae from Domesticated Broodstock Trijuno, Dody Dharmawan; Fujaya, Yushinta; Agviranti, Agviranti; Marhama, Syamsurya
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Blue swimming crab (Portunus pelagicus) is the important world fishery resource, but the crab larval rearing faces high mortality problem. The aim of the research was to compare survival and growth rate of larvae resulted from wild and domesticated broodstock blue swimming crab. Domesticated and wild broodstock were used to produce larvae. Domesticated broodstock was selected from repeated reared crab until third generation. Selection of the broodstock mainly based on the survival and growth rate. The wild broodstock was collected from Makassar Strait Sea. Larvae from the two sources of broodstock were reared in the conical tanks with density of 50 zoeas per liter. After reaching megalopa phase, then, the larvae were transfered into concrete tank completed with shelter and it were reared until crab phase. Crab phase was reared for 18 days, then these seed were released into the brackishwater pond for further domestication step. Larva from zoea to crab were fed with rotifer, artemia, fresh small shrimp and diet. The result showed that domesticated broodstock produced higher survival rate (P<0.05) larvae, faster larval stage changes (P<0.01) and uniform larval growth compared to the larvae from wild broodstock. It is clearly shown, the use of domesticated broodstock of blue swimming crab produced better larva quality and quantity than the wild broodstoock crab.Keywords : blue swimming crab, larvae, domestication, broodstock selection, faster growth

Mapping Embryogenesis in the Early Phases of Seabass (Lates calcarifer) Eggs on Different Salinities Djawad, Muhammad Iqbal; Tenriajeng, Muhammad Al Ghiffari; Trijuno, Dody Dharmawan
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 3 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.3.351-358

Embryogenesis is a critical stage in the development of fish eggs, as it determines the successful hatching and survival of larvae. Understanding the effects of salinity on embryogenesis is crucial for optimizing hatchery practices and improving the production of fish larvae, such as the seabass. The purpose of this study was to map the stages of embryonic development against various salinities in order to identify the ideal salinity for seabass egg hatching. Eggs from natural spawning in a maintenance bath were used in this investigation. Four containers with salinity treatments (20 ppt, 25 ppt, 30 ppt, and 35 ppt) are used to lay fertilized eggs. The findings demonstrated that, in comparison to 20 ppt and 25 ppt treatments, eggs at 30 ppt and 35 ppt treatments generated faster embryonic development stages. Different incubation salinities have a highly substantial effect on the hatching speed of seabass eggs. Further testing revealed that the eggs hatched in 14 h and 40 min, which had the fastest seabass roe hatching time (35 ppt), and 15 h and 20 min for the 20 ppt salinity treatment. The 35 ppt salinity treatment had the highest hatchability rate (80.67%), while the 20 ppt salinity treatment had the lowest percentage (71.78%). It can be concluded that a salinity of 35 ppt provides a good embryo development response where there are no embryos that fail to develop, have the shortest hatching times of 14 h and 40 min, and produce the highest hatchability of eggs compared to other salinities.

Perubahan Jaringan Hati Ikan Pari Kembang ( DASYATISKUHLI) Akibat Merkuri (HG) Tresnati, Joeharnani; Trijuno, Dody Dharmawan; Djawad, Iqbal
Torani Journal of Fisheries and Marine Science Vol. 24 No. 1 (2014)
Publisher : Hasanuddin University

Blue Spotted Ray (Dasyatis kuhlii) as the demersal organisms has a lot of accumulation of pollutants in the water that settles to the bottom. This study was conducted to look at the liver tissue alteration of Blue Spotted Ray caused by contamination of mercury (Hg). Performed on 5 tanks. Tank A as a control, tank B with concentration 0.025 ppm, tank C with concentration of 0.05 ppm, tank D with concentration 0.1 ppm and tanks E with concentration 0.2 ppm. The result is alteration on the liver tissue in the form of atrophy, cloudy swelling, vacuolization degeneration and even necrosis or death of liver tissue already. The alteration can reduce and eveneliminate the function of the liver as a toxin absorbent and can lead to death.

Effectivity of mulberry leaf extract on stimulating ekdisteroid hemolimph content and molting of mud crab (Scylla olivacea) Fujaya, Yushinta; Trijuno, Dody Dharmawan; Haryati, Haryati; Hasnidar, Hasnidar; Rusdi, Muhammad; Usman, Zainal
Torani Journal of Fisheries and Marine Science VOLUME 2 NOMOR 1, DESEMBER 2018
Publisher : Hasanuddin University

This study aims to study the performance of phytoecdisteroids from mulberry leaves in molting stimulating of mangrove crabs. The research was carried out at the Research and Development Center for Aquaculture at the Faculty of Marine Sciences and Fisheries at Hasanuddin University, located in Bojo Village, Mallusetasi District, Barru Regency, South Sulawesi Province. Mangrove crabs (Scylla olivacea) weighing 45-55 g and carapace widths 60-65 mm were used as test animals. There were five doses of mulberry leaf extract applied through feed, namely: A) 1.1 mg / g feed, B) 1.9 mg / g feed, C) 2.7 mg / g feed, D) 3.5 mg / g feed. Crabs were kept individually in plastic boxes that are floated above the surface of the pond. During rearing, crabs are fed 3% dry fish per day which has been enriched with mulberry leaf extract. The parameters observed were ecdysteroid compounds contained in mulberry leaf extract, ecdysteroid content in hemolymph before and after application of mulberry leaf extract, and molting percentage. Identification of the ekdisteroid compounds contained in mulberry leaf extract was carried out using Thin Layer Chromatography (TLC) and Measurement of the content of ecdysteroids in crab hemolymh was carried out using Ultra Fast Liquid Chromatography (UFLC). The results showed that the 3.5 mg dose of mulberry leaf extract / g feed gave the highest increase in ecdysteroid hemolymph, which was approximately 1760 ekdisteroid per mL hemolimph, while the dose of 1.1 mg / g feed only provided an increase of 100 ng ekdisteroid per mL hemolymph. The higher the dose of mulberry leaf extract in the feed, the higher the increase in the concentration of hemolymph ecdysteroids, but the high concentration of ecdystoid in the hemolymph does not guarantee molting. The optimal dose of mulberry leaf extract in the feed stimulating molting was 2.4 mg / g of feed.Keywords: mulberry, phytoecdysteroid, molting, crab, aquaculture

The effectiveness of immersed in extract viscera of sea cucumber Holothuria sp on growth, consumption level and feed efficiency in tilapia larvae Oreochromis niloticus Hidayani, Andi Aliah; Achmad, Marlina; Trijuno, Dody Dharmawan; Suwanmala, Jitima; Jannati, Alifah Nurul; Rewa, Atira; Auliah, A. Dyar Fadya
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.148-157

The aim of this research was to determine the effect of sea cucumber immersion extract on growth, consumption and feed efficiency in masculinized tilapia larvae aged six to seven days. September–November 2022 was the time the research was carried out and the experimental method used in this study was a Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the soaking of sea cucumber viscera with doses of 0 ml/L (A), 1 ml/L (B), 3 ml/L for 24 hours (C), 5 ml/L for 24 hours (D), and 0.5 ml/L 17α-MT for 12 hours as positive control (E), then maintained for 60 days. Parameters observed included absolute length growth, absolute weight growth, feed consumption level, feed efficiency and water quality. The results showed that immersion sea cucumber innards for 24 hours with a dose of 1 ml/L showed the highest growth rate in absolute length and a dose of 3 ml/L showed the highest feed consumption rate. Keywords: feed consumption rate, feed efficiency, sea cucumber viscera, tilapia fry ABSTRAK Tujuan dari penelitian ini adalah menentukan pengaruh ekstrak jeroan teripang terhadap pertumbuhan, konsumsi dan efisensi pakan pada larva ikan nila hasil maskulinisasi yang berumur enam sampai tujuh hari. Penelitian ini dilakukan pada bulan September–November 2022 dan metode eksperimen yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah perendaman jeroan teripang dengan dosis 0 ml/L (A), 1 ml/L (B), 3 ml/L (C), 5 ml/L selama 24 jam (D), dan 0,5 ml/L 17α-MT selama 12 jam sebagai kontrol positif (E), kemudian dipelihara selama 60 hari. Parameter yang diamati meliputi pertumbuhan panjang mutlak dan bobot mutlak ikan, tingkat konsumsi pakan, efisiensi pakan dan kualitas air. Hasil penelitian menunjukkan perendaman jeroan teripang selama 24 jam dengan dosis 1 ml/L menunjukkan laju pertumbuhan panjang mutlak yang tertinggi dan pada dosis 3 ml/L tingkat konsumsi pakan dan efsiensi pakan tertinggi. Kata kunci: benih ikan nila, efisiensi pakan, jeroan teripang, tingkat konsumsi pakan

Co-Authors Agviranti, Agviranti Ahmad, Fakhirah Andi Aliah Hidayani Andi Parenrengi Andi Tenriulo Asphama, Andi Ivo Auliah, A. Dyar Fadya Haryati Haryati Hasnidar Hasnidar Iqbal Djawad, Iqbal Jannati, Alifah Nurul Joeharnani Tresnati Marhama, Syamsurya Marlina Achmad Muhammad Iqbal Djawad Muhammad Rusdi Rewa, Atira Siti Aslamyah Suwanmala, Jitima Tenriajeng, Muhammad Al Ghiffari Yushinta Fujaya Zainal Usman

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