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Yulianti Fauziah
Politeknik Bina Husada Kendari
Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Journal : Jurnal Analis Kesehatan Kendari

 1 
UJI AKTIVITAS ANTIBAKTERI LENDIR BEKICOT (Achatina fulica) TERHADAP BAKTERI Staphylococcus epidermidis Yulianti Fauziah
Jurnal Analis Kesehatan Kendari Vol. 4 No. 1 (2021): Jurnal Analis Kesehatan Kendari (JAKK : Vol. 4 (1) Desember 2021)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v4i1.172

Abstract

ABSTRAK Bekicot merupakan sumber protein yang berkualitas baik. Lendir bekicot mengandung zat beta aglutinin sebagai antibodi di dalam plasma (serum), glikokonjugat, acharan sulfat dan protein achasin sebagai peptida antibakteri. Penelitian ini bertujuan untuk mengetahui zona hambat serta efektivitas lendir bekicot (Achatina fulica) pada pertumbuhan bakteri S. epidermidis. Jenis penelitian yang digunakan adalah penelitian eksperimen yang terdiri atas lima perlakuan dan tiga kali pengulangan. Sampel uji yang diteliti adalah lendir bekicot (Achatina fulica) yang dilarutkan dalam aquadest hingga didapatkan varian konsentrasi 10%, 20%, dan 30%. Hasil penelitian menunjukkan bahwa lendir bekicot menghambat pertumbuhan bakteri S. epidermidis masing-masing konsentrasi 10%, 20% dan 30% yakni 2,66 mm, 6,9 mm dan 7,6 mm. Berdasarkan uji Anova mendapatkan hasil F hitung yaitu 37,16 lebih besar dibandingkan dengan F tabel 3,47 kemudian dilanjutkan dengan uji BNT untuk mengetahui konsentrasi lendir bekicot (Achatina fulica) yang efektif dalam menghambat pertumbuhan S. epidermidis. Berdasarkan hasil penelitian dapat disimpulkan bahwa lendir bekicot (Achatina fulica) dapat menghambat pertumbuhan bakteri S. epidermidis. Dari hasil uji Anova dan uji lanjutan BNT konsentrasi lendir bekicot yang lebih efektif dalam menghambat pertumbuhan bakteri S. epidermidis yaitu pada konsentrasi 30%.Kata Kunci : Bekicot, Lendir Bekicot, Achatina fulica, Antibakteri ABSTRACTSnails are the high quality source of protein. snail mucous contains beta-aglutinin as an antibody in plasma (serum), glykokonjugate, acharan sulfate and achasin protein as antibacterial peptide. This study aims to determine the inhibition zone and the effectiveness of snail mucous (Achatina fulica) on the growth of S. Epidermidis bacteria. This examination used experimental research consisting of five treatments and three repetitions. The samples test examined were snail mucous (Achatina fulica) dissolved in aquadest to obtain the concentation variation of 10%, 20% and 30%. The result of this examination shows that snail mucous inhibits the growth of S. Epidermidis bacteria respectively concentrations of 10%, 20% and 30% are 2,66 mm, 6,9 mm dan 7,6 mm. Based on Anova test the researcher got the result of F count 37,16 is bigger than F table 3.47 then proceed with BNT test to know the concentration of the effective snail mucous (Achatina fulica) to the growth of S. Epidermidis bacteria. Based on the result, it can be concluded that snail mocous (Achatina fulica) can inhibit the growth of S. Epidermidis bacteria from the result of Anova and then proceed with BNT test on snail mucous consentration is more effective in inhibiting the growth of S. Epidermidis bacteria that is in concentration of 30 %. Keywords : Snail, Snail Mucous, Achatina Fulica, Antibacterial.
UJI DAYA HAMBAT EKSTRAK ETANOL BINTANG LAUT BERTANDUK (Protoreaster nodosus) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus yulianti fauziah; Randa Wulaisfan
Jurnal Analis Kesehatan Kendari Vol. 3 No. 2 (2021): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 3 (2) Juni 2021)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v3i2.182

Abstract

ABSTRAK Bintang laut bertanduk merupakan salah satu biota laut yang digunakan sebagai obat tradisional. Salah satunya adalah pengobatan infeksi. Infeksi merupakan salah satu penyebab penyakit yang sering terjadi di daerah yang beriklim tropis khususnya Indonesia. Salah satu infeksi yang sering terjadi adalah infeksi pada kulit yang disebabkan oleh bakteri Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol bintang laut bertanduk dapat menghambat pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimental yang terdiri atas lima perlakuan dan tiga kali pengulangan dengan menggunakan metode pengujian paper disk. Sampel uji yang diteliti adalah bintang laut bertanduk (Protoreaster nodosus) yang dilarutkan dalam DMSO 10% hingga didapatkan varian konsentrasi 5%, 10%, dan15% kemudian dilakukan uji daya hambat terhadap bakteri Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak bintang laut bertanduk menghambat pertumbuhan bakteri Staphylococcus aureus masing-masing konsentrasi 5%, 10% dan 15% yakni 5,99mm, 6,69mm dan7,64mm. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bintang laut bertanduk (Protoreaster nodosus) dapat menghambat pertumbuhan bakteri Staphylococcus aureus secara signifikan. Kata kunci :Zonahambat, Bintang Laut Bertanduk, Staphylococcus aureus. ABSTRACT Horned starfish are one of the marine biota used as traditional medicine. One of them is the treatment of infections. Infection is one of the causes of diseases that often occur in the tropics, especially Indonesia. One infection that often occurs is an infection of the skin caused by Staphylococcus aureus. This study aims to determine whether the horned starfish ethanol extract can inhibit the growth of Staphylococcus aureus bacteria. This type of research used in this study is an experimental study consisting of five treatments and three repetitions using a diskette testing method. The test sample studied was a horned starfish (Protoreasternodosus) dissolved in DMSO 10% to 5%, 10%, and 15% of the variant of concentration was obtained then the inhibitory test was performed on Staphylococcus aureus bacteria. The results showed that the horned starfish extract inhibited the growth of Staphylococcus aureus, each concentrations of 5%, 10% and 15% respectively 5.99 mm, 6.69 mm and 7.64 mm. Based on these results it can be concluded that the ethanol extract of the horned starfish (Protoreasternodosus) can significantly inhibit the growth of Staphylococcus aureus bacteria. Keywords: Inhibited zone, Horn Star, Staphylococcus aureus.
FORMULASI LOTION EKSTRAK DAUN Meistera chinensis SEBAGAI TABIR SURYA Meistera chinensis LEAF EXTRACT LOTION FORMULATION AS SUNSCREEN Nirwati rusli; yulianti fauziah; Elviyanti Yusdin
Jurnal Analis Kesehatan Kendari Vol. 4 No. 2 (2022): Jurnal Analis Kesehatan Kendari (JAKK : Vol. 4 (2) Juni 2022)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v4i2.189

Abstract

Excessive sunlight can have a negative impact on skin health, but the bad effects of sunlight can be overcome by using sunscreen preparations. One of the natural ingredients that can be used in the manufacture of lotion sunscreenisleaf Meistera chinensis. Meistera chinensis is a plant that belongs to the Zingiberaceae family. Previous research has stated that Zingiberaceae species such as Etlingera elatior (torch ginger) have one of the functions as antioxidants. This study aims to formulateleaves Meistera chinensis as sunscreen and determine the SPF value obtained. Leaf extract was Meistera chinensis extracted using maceration method using methanol as solvent. Physical evaluation tests, stability tests, and sunscreen activity were carried out at three concentrations, namely 1% (F1), 3% (F2) and 5% (F3) concentrations. The three formulations are pale green, green, and dark green in color, have a melon flavor and have a liquid and semi-solid texture, are homogeneous with an oil-in-water (W/A) emulsion type and meet the pH requirements of 6 and 7. The stability test results show that the three formulas do not stable because it is not homogeneous and there is a change in aroma after the cycling test. The SPF value increased along with the increase in extract concentration, namely 7.46 (extra protection), 14.12 (maximum protection), and 19.06 (ultra protection). Keywords: Extract, Leaf Meistera chinensis, Lotion, Sunscreen, SPF
Co-Authors Adryan Fristiohady Elviyanti Yusdin Eny Nurhikma Esti Badia Gusti Ray Sadimantara Hasnawati Hasnawati Idin Sahidin Inggit Suryaningsih Karmilah Karmilah Lisa Wulansari Siregar Muh. Arnas Muh. Azdar Setiawan Muh. Ilyas Yusuf Muhammad Hajrul Malaka Musdalipah Musdalipah Nirwati Rusli Nur Saadah Daud Nurhikma Nurhikma Nursaadah Daud Randa Wulaisfan Reymon Rifcha Selviana Puput Selfyana Austin Tee Sernita Sernita Syaiful Saehu Wahyuni Wahyuni Yodha, Agung Wibawa Mahatva