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All Journal Public Health of Indonesia JURNAL PENDIDIKAN TAMBUSAI INTERNATIONAL JOURNAL OF NURSING AND MIDWIFERY SCIENCE (IJNMS) Adi Husada Nursing Journal Jurnal Medika Cendikia (e-Journal) JPKM: Jurnal Profesi Kesehatan Masyarakat Al Jahiz: Journal of Biology Education Research Prima Wiyata Health Literasi Kesehatan Husada: Jurnal Informasi Ilmu Kesehatan Jurnal Multidisipliner Kapalamada Indonesian Journal of Midwifery Today APMa Jurnal Pengabdian Masyarakat
Dwi Retno Wati
Program Studi Pendidikan Profesi Bidan, Sekolah Tinggi Ilmu Kesehatan Husada Jombang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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EXPLORING DNA ISOLATION TECHNIQUE: A GUIDE FOR EDUCATORS AND STUDENTS Siti Masruroh; Wati, Dwi Retno
Jurnal Multidisipliner Kapalamada Vol. 3 No. 04 (2024): JURNAL MULTIDISIPLINER KAPALAMADA
Publisher : Pusat Studi Ekonomi, Publikasi Ilmiah dan Pengembangan SDM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62668/kapalamada.v3i4.1411

Abstract

This study is intended to guide educators or students who will conduct DNA isolation experiments to prove and view the presence of DNA contained in fruit using a simple method. DNA isolation is a method to obtain or research deoxyribonucleic acid from an organism. The basic principle in DNA isolation is to break or extract a tissue so that a cell extract contains DNA, RNA, and other essential substances will be formed. DNA isolation experiment is intended to determine a simple method of isolating DNA in fruit and to determine the effectiveness of detergents used for DNA isolation. In this experiment, to observe DNA, DNA isolation was carried out on the crushed fruits to form juice from the fruit. Then, make a solution from a mixture of detergent and water. Later, mix the fruit juice with a detergent solution and add salt to it. Once the solution is homogeneous, filter it and add cold ethanol. Then, the DNA strands will appear because of the reaction. The results obtained are influenced by the type of detergent used, the type of fruit used, the alcohol concentration, and the results of the filtering performed.
Pengaruh Pemberian Konseling ASI Eksklusif terhadap Efikasi Diri Ibu Menyusui Wati, Dwi Retno; Sumini, Gempi Tri; Suryani, Lilis; Sari, Wahyu Anjas
Jurnal Pendidikan Tambusai Vol. 9 No. 2 (2025): Agustus
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i2.30802

Abstract

Air Susu Ibu eksklusif (ASI eksklusif) merupakan makanan terbaik bayi untuk kebutuhan pertumbuhan dan perkembangannya di enam bulan pertama. Pada ibu menyusui, dibutuhkan keyakinan dan efikasi diri yang tinggi bahwa mereka dapat memberikan ASI bagi bayi mereka. Pemberian layanan konseling memungkinkan ibu memperoleh pemahaman komprehensif mengenai teknik menyusui yang benar dan manfaat ASI bagi bayi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian konseling ASI eksklusif terhadap efikasi diri ibu menyusui. Penelitian ini merupakan penelitian pra-eksperiment dengan rancangan one group pretest-postest. Sampel dalam penelitian ini sejumlah 33 ibu menyusui eksklusif ≤ 6 bulan yang merupakan total pupulasi. Data yang diperoleh diolah dan di analisis dengan uji Wilcoxon Signed Rank test. Hasil analisis uji statistik menunjukkan perubahan efikasi setelah konseling sebesar 15,50 dengan nilai signifikansi (Sig.) sebesar 0,000. Artinya, ada pengaruh pemberian konseling ASI eksklusif terhadap efikasi diri ibu menyusui. Kesimpulan penelitian ini adalah ada pengaruh pemberian konseling ASI eksklusif terhadap efikasi diri ibu menyusui. Diharapkan penelitian ini dapat dijadikan informasi untuk melakukan asuhan kebidanan, sehingga petugas dapat memberikan pelayanan yang maksimal guna meningkatkan mutu pelayanan kesehatan terutama konseling dan pemberian informasi mengenai pentingnya ASI eksklusif supaya tidak terjadi drop out dan pemberian ASI berlanjut sampai 2 tahun.
Implementasi Acupressure ST36, SP6, CV12 sebagai Upaya Meningkatkan Berat Badan Balita Wati, Dwi Retno; Sumini, Gempi Tri; Suryani, Lilis; Taninas, Ewalde Maria; Anjas Sari, Wahyu Anjas
APMa Jurnal Pengabdian Masyarakat Vol. 5 No. 2: Juli 2025
Publisher : STIKES Bhakti Husada Mulia Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47575/apma.v5i2.724

Abstract

Tujuan kegiatan ini adalah untuk meningkatkan berat badan balita melalui teknik akupresur pada titik ST36, SP6, dan CV12. Intervensi dilakukan selama 30 hari pada 20 balita usia 13–36 bulan di Desa Tembelang. Pelatihan diberikan kepada ibu balita untuk melakukan akupresur mandiri. Hasil analisis uji Wilcoxon menunjukkan peningkatan signifikan berat badan (p= 0,014). Teknik ini diharapkan dapat menjadi pendekatan alternatif dalam mendukung status gizi balita. Kegiatan ini terdiri dari melatih ibu balita untuk dapat melakukan acupressure secara mandiri, pengukuran berat badan sebelum dan setelah dilakukan acupressure selama 30 hari. Hasil dari kegiatan implementasi acupressure dapat meningkatakan berat badan balita, dengan kenaikan rata- rata 296 gram dengan Nilai p value yaitu 0.014. Setelah kegiatan ini diharapkan acupressure secara mandiri oleh keluarga balita dapat dilakukan secara rutin sebagai upaya pendekatan alternatif untuk membantu meningkatkan berat badan balita.
EFFECTIVENESS OF A COMBINED LABOR DANCE AND BIRTH BALL INTERVENTION IN REDUCING THE DURATION OF THE ACTIVE PHASE OF THE FIRST STAGE OF LABOR Tanlain, Dolfina Welmina Juliana; Sumini, Gempi Tri; Wati, Dwi Retno; Hidayati, Nurul
PRIMA WIYATA HEALTH Vol 7 No 1 (2026): Prima Wiyata Health: Januari 2026
Publisher : LPPM STIKES HUSADA JOMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60050/pwh.v7i1.106

Abstract

Background: Prolonged labor remains a significant contributor to maternal and neonatal complications, particularly during the active phase of the first stage of labor. Non-pharmacological interventions, such as labor dance and birth ball exercises, have been proposed to facilitate labor progress by enhancing maternal comfort and promoting effective uterine contractions. Objective: This study aimed to examine the effectiveness of a combined labor dance and birth ball intervention in reducing the duration of the active phase of the first stage of labor. Methods: A quasi-experimental study with a post-test-only control group design was conducted among 30 laboring women in the active phase of the first stage of labor. Participants were selected using purposive sampling and assigned to either an intervention group, which received a combination of labor dance and birth ball exercises, or a control group that received standard care. The duration of the active phase was measured using an observation sheet and analyzed using an independent t-test. Result: The results demonstrated a statistically significant difference in the duration of the active phase between the intervention and control groups (p = 0.02). Women who received the combined labor dance and birth ball intervention experienced a shorter active phase compared to those in the control group. Conclusion: The combination of labor dance and birth ball exercises is effective in shortening the duration of the active phase of the first stage of labor. This intervention may serve as a safe, feasible, and non-pharmacological alternative to support normal labor progress in maternity care settings.
Effectiveness of Oketani Breast Massage with Lavender Essential Oil in Enhancing Breastfeeding Success: A Quasi-Experimental Study in Rural Community Sumini, Gempi Tri; Suryani, Lilis; Sari, Wahyu Anjas; Wati, Dwi Retno
Public Health of Indonesia Vol. 12 No. 1 (2026): January - March
Publisher : YCAB Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36685/phi.v12i1.1275

Abstract

Background: Breastfeeding is a critical determinant of infant health and development, yet many mothers face challenges that hinder breastfeeding success, particularly in rural settings with limited access to lactation support. Oketani breast massage and lavender essential oil aromatherapy have been explored individually for their potential to improve lactation outcomes. Objective: This study aimed to evaluate the effectiveness of Oketani breast massage combined with lavender essential oil in enhancing breastfeeding success among postpartum mothers in a rural Indonesian community. Methods: A one-group pretest-posttest quasi-experimental design was used, involving 80 postpartum mothers in Penjalian Village, East Java, Indonesia. Participants received Oketani breast massage and lavender essential oil therapy daily for seven days postpartum. Breastfeeding success was assessed using the LATCH scoring system before and after the intervention. Data were analyzed using the Wilcoxon signed-rank test. Results: The proportion of mothers achieving a “good” LATCH score increased from 46.25% before the intervention to 72.50% after the intervention. The Wilcoxon test revealed a statistically significant improvement in breastfeeding success (Z = -4.750, p < 0.001), indicating the positive impact of the combined intervention. Conclusion: Oketani breast massage combined with lavender essential oil is an effective, low-cost, and culturally appropriate intervention to enhance breastfeeding success in postpartum mothers. Its implementation in rural communities can serve as a practical strategy to support exclusive breastfeeding practices and improve maternal-infant health outcomes. Keywords: oketani breast massage; lavender essential oil; breastfeeding success; postpartum mothers; LATCH Score
Co-Authors Anjas Sari, Wahyu Anjas De Yesus, Aljira Kristina Elis Fatmawati Forniawan, Ary Gempi Tri Sumini Gusfianti, Elsa Hany Puspita Lilis Suryani Lilis Suryani Nurul Hidayati Siti masruroh Siti Mudrikatin Siti Nur Farida Sofa Qurrata A'yun Sugiyanta Sugiyanta Taninas, Ewalde Maria Tanlain, Dolfina Welmina Juliana Wahyu Anjas Sari