Nanang Supena
Indonesian Oil Palm Research Institute

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JARAK GENETIK 47 AKSESI PLASMA NUTFAH KELAPA SAWIT (Elaeis guineensis Jacq.) ASAL KAMERUN BERDASARKAN KARAKTER MORFOLOGI Sujadi Sujadi; Tiara S Wandita; Nanang Supena; Yurna Yenni
Jurnal Penelitian Kelapa Sawit Vol 27 No 1 (2019): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1248.501 KB) | DOI: 10.22302/iopri.jur.jpks.v27i1.70

Abstract

Oil palm (Elaeis guineensis Jacq.) is a plantation commodity that has an important role in various aspects of life in Indonesia, especially the domestic economy. This is supported by the increasing demand for world palm oil for food needs (edible oil), industrial (oleochemical), and alternative energy sources based on biodiesel. The development of the palm oil industry requires several efforts to achieve increased national productivity, one of which is the utilization of quality seeds supported by the availability of genetic resources (germplasm) that have a high level of genetic diversity. Efforts that can be made for the development of oil palm in Indonesia is through the characterization of oil palm intoduction from abroad. Considering this, further research on genetic distance analysis of palm oil accession introduced from Cameroon is based on morphological characters to produce parent elders that can produce heterosis properties. Research conducted at Seed Garden of Adolina PTPN IV and laboratory analysis of plant material of Palm Oil Research Center located on Jl. Brigjen Katamso No. 5, Medan, North Sumatera, which lasted from December 2017 to February 2018. Single observations were made on 47 accession palm oil from Cameroon planted on December 2010 and ten trees from PPKS 540 variety for compare. Data analysis used was a description of the plant to know the character of plant morphology as well as genetic distance analysis. Analysis of genetic distance using PCA analysis and cluster analysis. The results obtained are based on the results of PCA (Principal Component Analysis) reduce the observed character into six major components that have eigen value > 1 and able to explain the material diversity tested for 73.8%. Based on the cluster analysis obtained the genetic distance of 47 accessions of palm oil from Cameroon by 57%. It can be concluded that, when the genetic distance between accessions is further away, the larger the genetic variability between the observed characters. If the genetic diversity is wider the greater the chance for successful selection in increasing the desired gene frequency.
KARAKTERISTIK PERKEMBANGAN BUNGA DAN BUAH 35 AKSESI ANGOLA KOLEKSI PUSAT PENELITIAN KELAPA SAWIT DI KEBUN ADOLINA PT PERKEBUNAN NUSANTARA IV Sujadi Sujadi; Nanang Supena; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 27 No 2 (2019): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4462.56 KB) | DOI: 10.22302/iopri.jur.jpks.v27i2.76

Abstract

Information of oil palm flower and fruit development is very important in the observation plant morphology. This observation was carried out to complete information on vegetative and generative developments which are very useful for oil palm breeding. The success of plant breeding is measured from new varieties with specific advantages. This research used collections of Angola germplasm exploration which were planted in December 2012 at Adolina Estate of PT Perkebunan Nusantara IV. A total of 35 accessions were planted with RCBD designs in 2 trials. Vegetative observation per tree was carried out every year, since the plants were 2 years old. Observation of flower development is done by selecting 2 sample trees per accession by purposive sampling. A total of 70 trees were observed for flower development to become fruit every 10 days for each frond. Data on flower development were grouped by BBCH (Biologische Bundesantalt Bundessortenamt und Chemische Industrie) scale. In addition to the morphological and flower developmental stages, this study also calculated the age between developmental phases from the appearance of one leaf to the mature harvesting bunches in all midrib and tree samples. The results showed that generative development of the 35 Angolan accessions can be arranged according to the BBCH scale, beginning with the appearance of the first leaf, the appearance of the flower, female / male flowers blooming (receptic / anthesis), bunches formation to mature ripe bunches. AGO038 accession only requires 385 days (12.8 months), calculated from the time the first leaf appears to the mature ripe bunches, but AGO049 produces faster harvested ripe bunches when calculated from receptive female flowers of 144 days (4.8 months).
Studi Asosiasi Genom (GWAS) Komponen Tandan Populasi Interspesifik Elaeis oleifera dan Elaeis guineensis Heri Adriwan Siregar; Edy Suprianto; Sujadi Sujadi; Hernawan Y Rahmadi; Mohamad Arif; Retno Diah Setiowati; Nanang Supena; Rokhana Faizah; Sri Wening; Yurna Yenni; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 29 No 2 (2021): Jurnal Peneltian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i2.144

Abstract

The oil palm breeding program for the species Elaeis guineensis and the backcross Elaeis oleifera is running slowly because oil palm is an annual plant. Therefore, it is necessary to have an alternative approach that can accelerate the oil palm breeding program. The SNP (single nucleotide polymorphism) genome-wide approach was then used to study the association between 18 phenotypes of bunch component in oil palm germplasm of E. oleifera from Suriname and Brazil Coari, some interspecific hybrids and some elite progeny of E. guineensis. The genotyping by sequencing (GBS) analysis produced a total of 459 million or approximately 798 thousand reads per sample and 3,252 SNPs were eligible for 456 genotypes. Using various association models, eleven normalized phenotypic data showed significant associations with 29 SNPs. Based on the annotations, 17 SNPs were related to genes wtih certain biological functions. Three SNPs were found to be at the exon of a gene, namely SNP4416, SNP349 and SNP3865, while the other 15 SNPs were at the intragenic to a gene. Four SNPs are common SNPs in phenotypes C16:0 and C18:1 as weel as in C20 0 and C20:1. This research shows the potential of SNPs that can be used as an alternative approach to E. oleifera backcross breeding, although further research is needed for validation purposes.