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Retno Prihatini
Jurusan Biologi Universitas Andalas
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Other

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Morphology Character and Andrographolide Quantifications on Sambiloto  ( Andrographis paniculata (Burm.F.) Nees) Retno Prihatini; Auzar Syarif; Amri Bakhtiar
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202041107669-0-00

Abstract

ABSTRACTThe Morphology character and andrographolide quantification on Sambiloto (A. Paniculata) that growth in Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311)  have been studied.  The Morphology character  of  A. Paniculata on generative fase  have been done in the field and Laboratorium of Biology Department, Andalas University. Quantification of  andrographolide constituen were analysed by using High Performance Liquid Chromatography (HPLC) in Chemical of Nature Resources Laboratorium, Pharmacy Faculty, Andalas University. The result showed that Morphology character of  Sambiloto ( A. Paniculata ) include parameters  ie. range of height plant ( 27-45 cm) ; range of nodus numerous ( 10-14 ); range of leaf-7 size (length; width)  ( 3.8-4.1 ; 0.8-1.1) cm; range of branch numerous ( 8-10), leaf dry weight rate  (2.482 g ), stem dry weight rate  ( 5.882 g ), and day old flowering rate  ( 110 day ). The Result of quantification secondary metabolite with high economic value, namely andrographolide showed respectively with level  2.208% (vegetative) and  2.780 % (generative).  Key Words : Andrographis paniculata, morpology chracter, andrographolide quantification  ABSTRAKTelah dilakukan studi tentang Karakter morfologi dan kuantifikasi senyawa andrografolide pada tanaman Sambiloto (A. Paniculata) yang hidup di Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311). Karakter morfologi   A. Paniculata pada fase generative telah dilakukan di lapangan dan di  Laboratorium di Jurusan Biologi Universitas Andalas. Kuantifikasi senyawa andrografolid dianalisa dengan menggunakan High Performance Liquid Chromatography (HPLC) di Laboratorium Kimia Bahan Alam, Fakultas Farmasi Universitas Andalas.  Hasil menunjukkan bahwa karakter morfologi Sambiloto    ( A. Paniculata ) meliputi parameter  yaitu kisaran tinggi tanaman ( 27-45 cm) ; kisaran jumlah nodus ( 10-14 ); kisaran ukuran daunke-7  (panjang; lebar)  ( 3,8-4,1 ; 0,8-1,1) cm; kisaran jumlah cabang ( 8-10), rata-rata berat kering daun  (2,482 g ), rata-rata berat kering batang ( 5,882 g ), and rata-rata umur berbunga  ( 110 day ).  Hasil kuantifikasi metabolit sekunder dengan nilai ekonomi tinggi, yang dikenal sebagai senyawa andrografolid menunjukkan level   2,208%  (vegetatif) dan 2,780 % (generatif). Kata kunci: Andrographis paniculata, karakter morfologi, kuantifikasi andrografolid. 
King of Bitter (Andrographis paniculata Nees) Somatic Embryogeni Callus Culture on MS Medium With Added by 2,4-D and BAP Prihatini, Retno; K.Huda, Nadyatul; Idris, Muhamad
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.130950

Abstract

In this research we have studied about somatic embryogenesis derived on Sambiloto (Andrographis paniculata) cv. VGT callus culture by addition BAP (benzylamino purine) and 2,4-D (2,4 diclorophenoxyacetic acid)   on MS (Murashige and Skoog) Medium. King of Bitter seeds were germinated on MS medium for 3 weeks, then  the second leaf  that full opened used as explants. The explant planting  on medium with several concentration growth regulator (BAP combinated with 2,4-D) to callus induce.  MS medium  with added by 0.5 ppm BAP+ 0.5 ppm 2,4-D  ( A treatment), 0.5. ppm BAP + 1 ppm 2,4-D (B treatment ), 0.5 ppm BAP + 1.5 ppm 2,4-D (C treatment ), 0.5 ppm BAP + 2 ppm 2,4-D (D treatment), each of treatment with 5 replications. The observation used descriptive method for callus texture, somatic embryo derived with it development fase  after subcultured on MS medium with the same concrentration BAP  and 2,4-D . The result showed that callus  have formed on  all of treatment (A, B,C and D), whereas callus with somatic embryogenesis only on C treatment, with the range of callus fresh weight (FW) were 0.6-1.0 g.  After 3 weeks at second subcultured on MS medium with added 0.5 ppm BAP+ 1,5 ppm 2,4-D ,  somatic embryo  development showed  globular fase  and heart shape fase .
Co-Authors Aadrean Aadrean Agus Purwito Amri Bakhtiar Auzar Syarif K.Huda, Nadyatul Muhamad Idris, Muhamad Nurainas Nurainas Nurjaman, Muhammad Ratih Wulandari Rianto Rianto Sudirman Yahya Syamsuardi Syamsuardi Wijayanto, Ristu Aji Yoga Sahria