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All Journal Journal of Midwifery and Nursing JURNAL FARMASIMED (JFM) Jurnal Penelitian Farmasi dan Herbal Jurnal Pengabdian Masyarakat Putri Hijau Fundamental and Applied Research in Medicine and Allied Sciences Indonesia
Nina Irmayanti Harahap
Institut Kesehatan Deli Husada
Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Development of a Microchip Capillary Electrophoresis Method with Amperometric Detection for Rapid and Simultaneous Analysis of Ascorbic Acid and Glutathione in Antioxidant Cocktail Injection Preparations Nina Irmayanti Harahap
Fundamental and Applied Research in Medicine and Allied Sciences Indonesia Vol. 1 No. 1 (2025): May, 2025
Publisher : CV. Get Press Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69855/farmasi.v1i1.390

Abstract

Antioxidant cocktail injections containing ascorbic acid (AA) and glutathione (GSH) require precise and reliable analytical methods to ensure product safety and therapeutic consistency. Conventional methods such as HPLC tend to be time-consuming, require a large number of reagents, and are less suitable for rapid pharmaceutical quality control or at production sites. This study aimed to develop and validate a microchip capillary electrophoresis with amperometric detection (MCE-AD) method for the simultaneous quantification of AA and GSH in injectable preparations. Method optimization included the selection of running buffer, voltage, sample injection parameters, and detection potential to achieve stable separation and optimal electrochemical response. The validated method showed excellent linearity over the concentration range of 1–150 mg/L for AA and 2–200 mg/L for GSH, with correlation coefficients of 0.9989 and 0.9991, respectively. The detection limits were recorded at 0.42 mg/L for AA and 0.61 mg/L for GSH, while the quantification limits were 1.39 mg/L and 2.04 mg/L, respectively. Precision testing yielded a %RSD of <3%, and accuracy testing demonstrated recoveries between 97–103%. Analysis of commercial injection samples demonstrated compliance with pharmacopoeial requirements, supporting the practical application of this method. With an analysis time of <60 seconds and very low sample consumption (<10µL per analysis), the developed MCE-AD technique provides a rapid, economical, and reliable alternative to conventional analytical platforms. These findings confirm the suitability of the method for routine quality testing and its potential integration into portable and automated pharmaceutical analysis systems.
Integration of LC-HRMS-Based Metabolomics, INS-1 Cell Assays, and Molecular Docking to Elucidate the Synergistic Antidiabetic Mechanism of the Kunyit–Asam Herbal Formulation Muhammad Nurul Fadel; Emma Jayanti Besan; Nur Masyithah Zamruddin; Cut Intan Annisa Puteri; Nina Irmayanti Harahap
Fundamental and Applied Research in Medicine and Allied Sciences Indonesia Vol. 1 No. 1 (2025): May, 2025
Publisher : CV. Get Press Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69855/farmasi.v1i1.391

Abstract

Diabetes mellitus remains a major global health burden, leading to growing interest in herbal formulations as complementary antidiabetic therapies. This study aimed to elucidate the synergistic antidiabetic mechanism of the traditional Kunyit–Asam herbal formulation by integrating LC-HRMS-based metabolomics, in vitro INS-1 pancreatic β-cell assays, and molecular docking analysis. Metabolomic profiling using LC-HRMS identified a diverse array of bioactive secondary metabolites, predominantly phenolic acids, flavonoids, and curcuminoids, which are associated with antidiabetic and antioxidant activities. Functional evaluation in INS-1 cells demonstrated that the Kunyit–Asam extract significantly enhanced glucose-stimulated insulin secretion (GSIS) and improved β-cell viability, indicating insulinotropic and cytoprotective effects. Furthermore, molecular docking analysis revealed that several identified metabolites exhibited strong binding affinities toward key diabetes-related molecular targets, including α-glucosidase, α-amylase, AMP-activated protein kinase (AMPK), insulin receptor substrate-1 (IRS-1), phosphoinositide 3-kinase (PI3K), and GLUT4 regulatory proteins. Integration of metabolomic, biological, and in silico findings suggests that the antidiabetic activity of Kunyit–Asam is mediated through multi-target modulation of insulin signaling pathways, inhibition of postprandial glucose-regulating enzymes, and protection against oxidative stress. These results support a synergistic pharmacological mechanism driven by multiple phytoconstituents rather than a single dominant compound. This study provides scientific evidence supporting the traditional use of Kunyit–Asam as an antidiabetic herbal formulation and offers valuable insights for future herbal-based drug development.
UJI AKTIVITAS MOISTURIZER PROBIOTIK PRODUK KECANTIKAN TERHADAP BAKTERI Propionibacterium acnes dan Staphylococcus epidermidis Nina Irmayanti Harahap; elysa elysa; nurul dahlia harahap
Jurnal Penelitian Farmasi & Herbal Vol. 8 No. 2 (2026): Jurnal Penelitian Farmasi dan Herbal
Publisher : Fakultas Farmasi Institut Kesehatan DELI HUSADA Deli Tua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36656/jpfh.v8i2.2948

Abstract

Acne is one of the most common skin disorders affecting adolescents and young adults, with severity ranging from mild to severe. The pathogenesis of acne is influenced by multiple factors, including the presence of skin microbiota, particularly Propionibacterium acnes and Staphylococcus epidermidis. Objective: This study aims to evaluate the effectiveness of probiotics formulated in a moisturizer gel in inhibiting bacterial activity without compromising their moisturizing function. Methods: This study employed an experimental design to evaluate the antibacterial activity of moisturizer gel formulations using the well diffusion method, with inhibition zone diameter as the measured parameter. Additionally, a moisturizing test was conducted on 18 volunteers with dry skin conditions. Results: The results demonstrated that all moisturizer gel formulations exhibited moderate antibacterial activity, with inhibition zone diameters ranging from 5 to 10 mm. The moisturizing test conducted on 18 volunteers indicated that all formulations maintained skin moisture within the normal range, with values between 30% and 50%. Conclusion: Based on the antibacterial activity test, it can be concluded that probiotic-containing moisturizer gels exhibit antibacterial properties. However, their effectiveness remains moderate, as indicated by the inhibition zone diameters within the range of 5–10 mm.
Testing the Activity of Nanochitosan Hydrogel Containing Earthworm (Lumbricus rubellus) Extract as a Future Anti-Aging Candidate Nina Irmayanti Harahap; Nurul Dahlia Harahap; Angga Nugraha Sanjaya
Jurnal FARMASIMED (JFM) Vol 9 No 1 (2026): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v9i1.3820

Abstract

Background: Premature aging is a skin problem influenced by exposure to solar radiation and the formation of Reactive Oxygen Species (ROS). Accumulation of ROS can cause oxidative stress, leading to skin damage such as dryness, loss of elasticity, and wrinkles. Earthworm (Lumbricus rubellus) extract contains flavonoids, alkaloids, and tannins, which have potential antioxidant activity. Formulation in the form of a nanitosan hydrogel is expected to support the delivery of active compounds into the skin. Objective: This study aimed to analyze the antioxidant activity of Lumbricus rubellus extract and evaluate the physical characteristics and stability of nanitosan hydrogel as an anti-aging candidate. Methods: The extract was obtained by maceration using 70% ethanol. Phytochemical screening was conducted for flavonoids, alkaloids, and tannins. Antioxidant activity was evaluated using the DPPH method at concentrations of 20–100 µg/mL and determined based on the IC₅₀ value. The extract was then formulated into nanitosan hydrogel at concentrations of 2.5%, 5%, and 7.5%, followed by evaluation of organoleptic properties, homogeneity, pH, spreadability, viscosity, and irritation. Results: The extract yield was 34.3% and contained flavonoids, alkaloids, and tannins. The IC₅₀ value of the extract was 95.44 ppm, indicating strong antioxidant activity. The preparations showed good homogeneity and organoleptic stability, with a pH range of 6.6–6.8 and viscosity of 28,000–32,000 cps. Visual observation showed no apparent signs of irritation. Conclusion: Lumbricus rubellus extract demonstrated strong antioxidant activity and could be formulated into nanitosan hydrogel with good physical characteristics, indicating its potential for further development as an anti-aging preparation.
FORMULASI DAN EVALUASI AKTIVITAS ANTIOKSIDAN FACE MIST BUAH BIT MERAH (BETA VULGARIS L.) DENGAN METODE DPPH Elysa anggara; Rika Puspita Sari; Nina Irmayanti Harahap
Jurnal Penelitian Farmasi & Herbal Vol. 9 No. 1 (2026): Jurnal Penelitian Farmasi dan Herbal
Publisher : Fakultas Farmasi Institut Kesehatan DELI HUSADA Deli Tua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36656/gbsjm584

Abstract

Red beetroot (Beta vulgaris L.) contains antioxidant compounds such as flavonoids and betalain which have the potential to be used as active ingredients in cosmetic preparations. This study aimed to formulate red beetroot ethanol extract into a face mist preparation, evaluate the physical characteristics of the preparation, and determine its antioxidant activity using the 2,2 diphenyl -1-picrylhydrazyl (DPPH) method. This study was an experimental study with a face mist formulation that contains red beetroot ethanol extract at a concentration of 5% (F1), 10% (F2), and 15% (F3), as well as a formula without extract (F0) as a control. The evaluation included organoleptic test, homogeneity, pH, viscosity, spray power, dry time, and antioxidant activity based on the IC50 value.The study results show that all face mist formulas meet the requirements of physical evaluation of the preparation. Antioxidant activity testing showed consecutive IC50 values of 289,19 µg/mL (F0), 69,83 µg/mL (F1), 63,82 µg/mL (F2), and 51,59 µg/mL (F3). The higher the concentration of red beet fruit extract used, the stronger the antioxidant activity produced. F3 formula with 15% extract concentration provides the best antioxidant activity and is classified as strong, close to the very strong category. Based on the study results, it can be concluded that red beetroot ethanol extract (Beta vulgaris L.) has been successfully formulated into a face mist preparation that meets the requirements of physical quality and has good antioxidant activity. 15% extract concentration is the most effective formulation as an antioxidant. It is suggested that researchers can further develop red extract (Beta vulgaris L.) in other dosage forms and increased concentrations.
Co-Authors Elysa anggara Elysa Elysa Emma Jayanti Besan Harnis, Zola Efa Muhammad Nurul Fadel Nur Masyithah Zamruddin Nurul Dahlia Harahap nurul dahlia harahap Palas Tarigan Puteri, Cut Intan Annisa Putri Andini Rika Puspita Sari Sanjaya, Angga Nugraha Sofia Rahmi Sri Rezeky