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Optimation of β-mannanase production on submerged culture of Eupenicillium javanicum as well as pH and temperature enzyme characterizations Purwadaria, T; Haryati, T; Frederick, E; Tangendjaja, B
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 1 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.798 KB) | DOI: 10.14334/jitv.v8i1.372

Abstract

Two successive experiments were conducted to determine the optimal substrate concentration of coconut meal (CM) and incubation time for production of β-mannanase from Eupenicillium javanicum. Both experiments were designed based on factorial. In the first experiment, the main factor was substrate concentration of 1, 2, and 3%, while the sub-factor was incubation time of 1, 2, 3, 4, 5, 6, and 7 days. The two factors were interacted highly significantly (P<0.01). Since the highest β- mannanase activity, protein concentration and saccharification activity towards coconut meal were obtained from 3% CM after five day incubation time (P<0.05), the second experiment was designed for higher substrate concentration. The main factor was also substrate concentration of 3, 4 and 5%, while the sub factor was incubation time of 5 and 6 days. The two factors were also interacted highly significantly (P<0.01) for mannanase activity and protein concentration, while specific activity was not significantly different (P>0.05). The best activity was obtained at 4% of coconut meal for five day incubation time, which was not significantly different with that of 3% at the same incubation time. Therefore, it was concluded from both experiments that the best enzyme production was obtained from 3% of coconut meal at incubation time of 5 days. Then, further experiments show that the enzyme had optimum pH at the range of 5.4-5.8, the same pH range in duodenum, while at pH 4.5 the activity was relatively low. Although, at pH 4.5 the enzyme activity was reduced, the enzyme was still active for four hours. At pH 5.8 and 6.5 the enzyme was quite stable. The optimum temperature of the enzyme was at 500C, higher than the body temperature of most poultry (400C). The reduction of enzyme activity at 400C could be overcome by increasing the enzyme concentration. The enzyme was stable after 4 hour incubation at 28 (room temperature) and 400C, however, the enzyme activity was considerably reduced at temperature of 900C after 60 second incubation. In the poultry digestion system the activity is not affected by temperature, but in the pelleting process where the steam temperature approximately 900C has to be limited for not more than 30 seconds.   Key words: Coconut meal, β-mannanase, Eupenicillium javanicum, pH and temperature characterization
Response of broilers on the diet containing fermented palm oil sludge stored at various length of time Bintang, I.A.K; Sinurat, A.P; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.024 KB) | DOI: 10.14334/jitv.v8i2.375

Abstract

An experiment was conducted to study the response of broilers on the diet containing fermented palm oil sludge with Aspergillus niger stored at different length of time. The research was carried out in Research Institute for Animal Production Ciawi, Bogor. The experiment was conducted based on completely randomized design using 245 day old broiler chicks with 5 replicates. They were allotted to 7 diets containing one control without palm oil sludge (R0), 5 and 10% of fresh-fermented palm oil sludge (LSF) (R1 and R2), 5 and 10% of LSF stored at room temperature for 2 months (R3 and R4) and stored for 3 months (R5 and R6). Diets were fed ad libitum for 5 weeks. Feed intake, weight gain, feed conversion ratio, and carcass yields were observed as parameters. Results showed that no parameters were significantly affected by the treatments. It is concluded that 10% of fermented palm oil sludge could be included in diet of broilers even after three months storage at room temperature.   Key words: Broiler, fermented palm oil sludge, storage period
Effectivity of Aloe vera bioactives as feed additive for broilers reared on deep litter Sinurat, A.P; Purwadaria, T; Pasaribu, T; Rakhmani, W; Dharma, J; Rosida, J; Sitompul, S; ., Udjianto
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.675 KB) | DOI: 10.14334/jitv.v9i3.402

Abstract

There are plenty of Indonesian plants contain usefull bioactive components. One of them is Aloe vera. Previous experiment showed that Aloe vera bioactives reduced aerob bacteria in the intestinal and improved feed efficiency in broilers reared in cages. The results however, gave some variations, may be due to variation in rearing the chickens. Two experiments were carried out to study the effectivity of Aloe vera bioactives as feed additives for broilers reared on deep litter. In the first study, six experimental diets in mash form were formulated, i.e.: Control (K); K + antibiotic; K + 0.50 g/kg dry Aloe vera (LBK); K + 1.00 g/kg LBK; K + anthraquinone; K + Aloe vera in semi-liquid form. The amount of semi liquid Aloe vera and the anthraquinone were equally to 1.00 g LBK/kg. Results showed that antibiotic improved body weight gain 6.10% and feed efficiency 5.50% better than the control, although statistically not significant (P>0.05). Aloe vera bioactives in low doses (0.50 g/kg) also improved weight gain (6.30%) and feed efficiency (5.20%) similar to the antibiotic. However, Aloe vera in high doses and anthraquinone (equal to 1.00 g/kg diet) did not improve performance of broilers. There were no significant changes on carcass yield, abdominal fat levels, weight of liver, gizard and gastro intestinal tract due to any feed additives tested. The second experiment were carried out to study the effectivity of feed additives when included in crumble diets. Six experimental diets, i.e.: Control (K), K + antibiotic, K + Semi-liquid Aloe vera (equal to 1.00g dry Aloe vera/kg), K + 0.50 g dry Aloe vera/kg, K + 1.00 g dry Aloe vera/kg, K + 0.50 g dry Aloe vera + 0.50 g Curcuma xanthorrhiza meal/kg. All diets were fed in crumble form. Results showed that chickens fed with feed additives (antibiotic or Aloe vera bioactives) have a significantly (P<0.05) higher body weight gain and feed efficiency than those fed with control diet. The best weight gain was achieved by low dose dry Aloe vera (1342 g/bird) and the lowest was the control (1039 g/bird). The best feed conversion ratio was achieved by antibiotic treatment (1.756) followed by low dose dry Aloe vera + curcuma meal (1.758) and the worst was showed by the control (1.908). It is concluded that Aloe vera bioactives at low dose (0.50 g/kg diet) could improve body weight gain and feed efficiency of broilers reared on deep litter. The improvement achieved was similar as the antibiotic. The bioactive is more effective when fed in crumble diets.   Key words: Broilers, bioactives, Aloe vera
Preservation of semi-liquid Aloe gel: total phenolic and emodin content Rakhmani, Susana I.W; Sitompul, S; Rosida, J; Purwadaria, T; Sinurat, A.P
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 4 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.034 KB) | DOI: 10.14334/jitv.v9i4.431

Abstract

Semi liquid Aloe gel (LBSC) was preserved using sodium chloride (1, 2 and 3%) and sugar (10, 20 and 30%) for 7 weeks at 4ºC. Physical observations (color, odor and gas) and chemical analysis (total phenolic and emodin) were performed every week during preservation. Changing color was observed at the fourth week and until the seventh week. The total phenolic content decreased by 23.2, 12.9 and 19.0% in LBSC with 1, 2 and 3% salt respectively. The total phenolic content in LBSC with 10, 20 and 30% sugar decreased by 64.0, 53.8 and 30.4% respectively. The emodin content decreased by 60.7% for control (LBSC alone), but in LBSC with 1, 2 and 3% salt it was decreased by 26.2, 19.2 and 50.8% respectively. The emodin content in LBSC with 10, 20 and 30% sugar decreased by 70.5, 67.4 dan 82.4% respectively. Emodin was more unstable than total phenolic. Preservation using sugar was not preserve the availability of emodin, while salt could inhibit the decrease of emodin concentration in LBSC. Preservation of LBSC with 2% table salt gave the lowest decrease of emodin and total phenolic contentsKey words: Aloe vera, fenol, emodin, preservation
The effect of aloe vera bioactive level as feed additive on the egg performances of laying hens Bintang, I.A.K; Sinurat, A.P; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.926 KB) | DOI: 10.14334/jitv.v10i2.459

Abstract

A study on the use of aloe vera bioactives as feed additive in layer ration was conducted. One hundred and twenty pullets strain Isa Brown were allocated into 5 treatments with 6 replicates and 4 birds/replicate. The treatments were: control,control+antibiotic (50 ppm zinc bacitracin), and control+ aloe vera at three levels (0.25; 0.50 and 1.00 g/kg). The treatments were conducted in a completely randomized design. Parameter measured were first initial body weight, age at 1st lay, feed intake, egg weight, hen day (%HD) and feed conversion ratio. The results showed that antibiotic and aloe vera used as additive for 9 months production did not significantly (P<0.05) affect all parameter measured, except feed intake of hens fed diet containing 0.5 g/kg aloe vera was significantly (P<0.05) higher than control. The addition of aloe vera at 1.0 g/kg significantly (P<0.05) reduced the feed intake as compared with the control, aloe vera 0.25 and 0.50 g/kg. The used of aloe vera (1.00 g/kg) produced egg weight significantly (P<0.05) higher than the control, and feed conversion ratio was significantly (P<0.05) better than the control and aloe vera (0.25 g/kg). It is concluded that the best treatment was the diet with aloe vera level at 1.00 g/kg. This treatment improved feed efficiency 8.40%.     Key Words: Bioactive, Egg Production, Layer
The effectiveness of Aloe vera barbadensis bioactives on laying hens on commercial farmers Pasaribu, Tiurma; Sinurat, A.P; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (65.253 KB) | DOI: 10.14334/jitv.v11i2.511

Abstract

A field trial was conducted to study the effectiveness of dry gel Aloe vera (DG) as a feed additive for laying hens in commercial farms. The trial was consisted of two treatments, one was control, commonly used farmer ration containing antibiotic of zinc bacitracin at 0.5 g/kg and the second feed containing DG equal to 1.0 g/kg. Every treatment had two replicates with 504 Loghman laying hens. The hen day production (% HD), egg weight, feed consumption, feed conversion ratio (FCR), egg quality comprising yolk colour, albumin and yolk weights, egg shell eight and thikness, and mortality were observed for 24 weeks. The results showed that feed consumption, % HD, egg weight, FCR, yolk colour, albumin weight, yolk weight, egg shell weight, and egg shell thickness were not significantly different (P>0.05) between the control and DG treatment, except for the Haugh unit (HU). Thus, it can be concluded that Aloe vera bioactives has the same effectiveness as antibiotic as a feed additive at the level of commercial farms. Key Words: Laying Hens, Egg Production, Egg Quality, Aloe vera barbadensis Bioactives, Antibiotic
Nutritive values of solid heavy phase in replacing corn in broilers diet Sinurat, A.P; Purwadaria, T; Bintang, I.A.K; Pasaribu, T
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.878 KB) | DOI: 10.14334/jitv.v11i3.522

Abstract

Indonesia is still importing corn for feed. Utilization of nonconventional feedstuffs may reduce this importation. One of them is solid heavy phase (SHP), i.e., a solid substance gained after filtration of liquid waste of crude palm oil factory with a potential production approximately 2 million ton/year. This research was carried out to study the utilization of SHP to replace corn in broiler’s diet. The SHP was dried and analysed for its nutrient compositions. The metabolisable energy was measured by using hens. Feeding trial was then conducted by replacing corn with SHP in various levels, i.e., 0 (Control), 10, 20, 30, and 40%. All diets were formulated to be isocalori dan isonitrogen. Five hundred broilers DOC were used in this trial and fed for 5 weeks. The performances of the birds were obsereved as parameters. Results showed that the ash, minerals crude fibre and fat contents of the SHP were higher than the corn. The crude protein of the  SHP was similar to that of corn, but some amino acids content of the SHP were lower than the corn. The AME and TME values of SHP were 3271 and 3465 cal/g, respectively. Results of feeding trial showed that body weight of the birds were less as the level of SHP to replace corn increased in the diet. The FCR also showed impared as the levels of SHP increased in the diet. However, replacement of 10% corn with SHP did not significantly affect the body weight and FCR as compared with the control. Feed consumptions of the birds were not significantly affected by replacing corn with SHP. Carcass yield and relative weights of some internal organs were not significantly different between dietary treatments. It is concluded that the solid heavy phase could be used to replace 10% corn in broiler’s diet. Key Words: Solid Heavy Phase, Palm Oil By Products, Broilers
Utilization off fermented copra meal with A. niger in broiler diet Haryati, T; Togatorop, M.H; Sinurat, A.P; Purwadaria, T; ., Murtiyeni
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.346 KB) | DOI: 10.14334/jitv.v11i3.524

Abstract

Research of nutritional evaluation of fermented copra meal and its application in broiler has been conducted. In the first experiment the crude protein and fiber contents were determined in the fermented copra meal using A. niger BPT, NRRL 337 and ES1. Further, 384 of Indian River DOC were used for in vivo experiment. Completed Randomized Design was used with 8 treatments: Control without and with 10% addition of copra meal, 3 levels of fermented copra meal addition with Aspergillus niger BPT or A. niger NRRL 337 (10, 15, 20%). Every treatment was repeated 8 time with 6 birds each. Variables measured were body weight, consumption, feed conversion, mortalities and carcass analyses. Diets were formulated in isocalori and isoprotein. The research was conducted in 6 weeks. The results showed that fermentation process increased the copra meal protein content from 22.3 to 36.1; 35.6 and 38.6% respectively for fermentation with A. niger BPT, NRRL 337 and ES1, decreased the crude fiber from 19.5 to 13.0; 18.9 and 8.28%. Results during 3 weeks trial show that the treatment significantly affected on broiler performance (P<0.05). Fermented copra meal was better than non fermented copra meal. There was no significantly different (P>0.05) in broiler performance at 6 weeks trial evaluation. The best conversion was in diet with 10% fermented copra meal with A. niger BPT and NRRL 337. The feed conversion of diets with 10% of each fermented products were better than control or diet without copra meal. The feed conversion of 15% fermented copra meal addition was not significantly different to control. Utilization of fermented copra meal with A. niger BPT or NRRL 337 should not more than 15%. Key Words: Copra Meal, Bioprocess, Broiler
Improving nutrient values of solid heavy phase for corn substitute in poultry diet Sinurat, A.P; Purwadaria, T; Bintang, I.A.K; Pasaribu, T
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.322 KB) | DOI: 10.14334/jitv.v12i2.545

Abstract

Solid heavy phase (SHP), a by product material of palm oil factory obtained by ceramic filtration from liquid waste could be produced approximately 2 million tons/year. The by product has a potential for substituting corn in poultry feed. A series of experiment was carried out to improve nutrient value of the SHP in order to obtain a feedstuff that can substitute corn in poultry feed. The SHP was processed by either fermentation or enzymatic process. The product was then dried and analysed for its nutrient values. Fermentation process was carried out by altering the dry matter of the substrate (40 or 50%), while enzymatic process was carried out by altering the dose and kind of enzymes used. The process that produced best nutrient values was considered for producing materials for a feeding trial. In this trial, the products were used in diet formulation to substitute 25 or 50% of the corn included in the control diet. The results showed that the fermentation processed could be conducted with dry matter of substrate at either 40 or 50%. The fermentation process significantly improved the nutrient values of the SHP as shown by decreasing the crude fibre and increasing the crude protein, amino acids and the ME value. The results also showed that the Balitnak enzyme (BS4) was optimum when added at 10 ml/kg dry matter SHP, while the commercial enzyme (EK) was optimum at level of 2 g /kg dry matter SHP. Results of feeding trial showed that 25% of corn in layer diet could be substituted with dried SHP or SHP + enzymes. This substitution tended to improve performances (egg production, egg weight and FCR) of the laying hens. Substitution of 25 or 50% corn with the fermented SHP tends to reduce the performance of the layinghens. Similar trend also occurred when 50% of the corn was substituted with the enzymaticly processed SHP. Key Words: Solid Heavy Phase, Palm Oil Waste, Fermentation, Enzymes, Laying Hens
Supplementation of Morinda citrifolia waste as bioactive compound on the performances of broiler Bintang, I.A.K; Sinurat, A.P; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.322 KB) | DOI: 10.14334/jitv.v12i1.556

Abstract

A study on the use of dried Morinda citrifolia waste as feed additive in broiler ration was conducted. One hundred day old chicks (doc) were allocated into 4 levels (control; 0.0; 1.2; 2.4 and 4.8 g/kg feed) of M. citrifolia waste with 5 replication. Each replication had 5 birds. The treatments were allocated in a completely randomized design. Variables measured were: feed intake, live weight, feed conversion ratio (FCR), percentages of carcass and internal organs ( liver. gizard, abdominal fat, and thickness of intestine). The results showed that M citrifolia waste supply did not significantly (P>0.05) affect all variables measured, but feed intake of those fed with high levels (4,8 g/kg) of M. citrifolia waste was significantly (P<0.05) lower than those fed with 1.2 g/kg. FCR was significantly (P<0.05 ) lower as compared to the control; 1.2 and 2.4 g/kg. It is concluded that the best treatment was the supplementation of M. citrifolia waste of 4.8 g/kg, this treatment improved feed efficiency by 5% as compared to the control. Key Words: M. citrifolia Waste, Performances, Broiler