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Broiler performance fed jatropha curcas seed meal detoxified by fermentation, physic and chemical treatments Wina, Elizabeth; Tangendjaja, B; Pasaribu, T; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

Utilization of jatropha seed meal as a feed ingredient is limited by the presence of several anti nutritive and toxic compounds in the seed meal. The aim of this research is to evaluate feeding of jatropha seed meal detoxified using fermentation by two fungi and rumen microbes (as biological detoxification) and using a combination of chemical and physical treatments on broiler performance. One hundred seventy five chicks (7 days old) were used and were divided into 5 treatments in 7 replications and each replication in one cage consisted of 5 chicks. R1 was control feed (K) without jatropha seed meal., 2) R2 was feed with 4% of Jatropha seed meal fermented by Neurosphora sitophila (FNS), R3 was feed with 4% of Jatropha seed meal fermented by Aspergillus oryzae (FAS), R4 was feed with 4% of Jatropha seed meal fermented by rumen microbes and R5 with 4% of Jatropha seed meal treated by autoclaved, refluxed by hexane and soaked in methanol (OEHM). Treated feed was given for 14 days at the end of the feeding treatment, two chickens from each replication were slaughtered and organ weights were recorded.Â Body weight of chicken and feed conversion ratio were calculated. The rest of the chicken was fed commercial feed for the next 7 days (recovery periode).Â Chicken mortality was almost 0% but 1 chicken from FAS treatment died at the recovery period. Feed consumption was lower at fermented jatropha seed meal than control (K) or OEHM, resulted in lower body weight of chicken. The abdomen fat weight and organ weights especially pancreas or spleen resulted from treatment with jatropha were much lower than that of K. In the recovery period, body weight of chicken in the OEHM treatment was almost similar from that of control chicken. In conclusion, biological detoxification on BBJ was able to reduce chicken mortality but could not improve the daily gain higher than control treatment. The best method to detoxify jatropha seed meal was the combination of physical and chemical treatment (using autoclave, followed by hexane and methanol extractions). Key Words: Broilers, Jatropha Seed Meal, Detoxification, Fermentation

Preservation of Bacillus pumilus PU4-2 xylanases by immobilization technique into pollard and cation addition Haryati, T; Marbun, P.A; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Utilization of by-product from agriculture as alternative source of feedstuff has been widely practiced. However their usage is limited due to high fiber content and low nutrient digestibility. The use of specific hydrolizing enzymes, xylanases are gaining importance because of their wide application in various industrial sectors especially in bioconversion of hemicellulosic material. This experiment was done to evaluate the effect of cation addition and immobilization of enzyme into pollard on stability of B. pumilus xylanase. The enzyme extract was purified by precipitation with 75% ammonium sulphate. Four kinds of cation (Ca2+, Fe3+, Mg2+, Zn2+) were added to the purified enzyme, at concentration of 1m M and stored at 4 and 27ËC. For immobilization process, the optimum enzyme concentration that will be added to pollard has been evaluated by analysis of xylanase activity and their recovery. The specific activity of enzyme after precipitation increased 1.8 times, from 420.3 to 765.2 U/mg protein. All cations act as activator which relative activity become 130.6; 139.0; 103.8 and 163.5% respectively. Concentration of 0.5mM Ca2+ and Fe3+ were most able to keep xylanases activity stable at 4ËC. The optimum composition of enzymes and pollard was 1.5 ml for 5 gram of pollard with recovery of xylanases activity of 82.2%. In immobilized enzyme, the activity of enzyme without cation addition is higher than that with addition of Ca2+ and Fe3+. Activity of enzyme stored at 4ËC is more stable than that at 27ËC. Immobilized enzyme is more stable for storage, which lasted for 7 weeks at 27ËC and 12 weeks at 4ËC compared to liquid enzyme which lasted for only 7 days at 27ËC and 13 days at 4ËC. Key words: Xylanase, Bacillus pumilus PU4-2, Preservation, Imobilization, Cation

Effect of fibre hydrolytic enzymes supplementation on performances of broiler chickens fed diets containing rice bran Ketaren, Pius P; Purwadaria, T; Sinurat, A.P; Haryati, T
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Use of the rice bran as poultry feed is restricted by its high fibre content as poultry is unable to hydrolyse the fibre into available energy. This experiment was aimed The aim of this experiment was to investigate the effect of crude enzymes supplementation on performances of broiler chickens fed diets containing 30% rice bran. The experiment was based on completely randomized design with nine experimental diets. Diets 1-5 contained 5% less than recommended protein and energy, based on determined metabolizable energy (ME) content in the rice bran at 2040 kcal/kg, without taking into account on the beneficial effect of the enzyme supplementation. These diets were: 1. A control diet (D2040, without enzymes); 2. D2040 + E. javanicum (BS4) + P. nalgiovense (S11); 3. D2040 + BS4 + A. niger; 4. D2040 + BS4 + commercial enzymes (CE); 5. D2040 +Â B. pumilus (PU4-2) + CE. Four other experimental diets contained the same protein and energy levels as in the diets 1-5, but had taken into account on the beneficial effect of the enzyme supplementation,. These diets were:Â 6. D2702 + BS4 + S11; 7. D2650 + BS4 + A. niger; 8. D2465 + BS4 + CE ;and 9. D2465 + PU4-2+ CE. Each experimental diet was repeated six times and each replication consists of 5 day-old broiler chickens. Results showed that FCR of the chickens fed D2040 + PU4-2 + CE Â diet (FCR = 1.38) and D2465 + PU4-2 + CE diet (FCR = 1.46) were significantly (P < 0.01) improved compared to the control dietÂ (FCR = 1.64). This experiment strongly shows that supplementation of PU4-2 + CE enzymes significantly (P < 0.01) improved feed efficiency of the broiler chickens by 11-16%. Key Words: Fibre Hydrolytic Enzymes, Broiler Chickens, Performances

Utilization of plant bioactives as feed additives for poultry: The effect of Aloe vera gel and its extract on performance of broilers Sinurat, A.P; Purwadaria, T; Togatorop, M.H; Pasaribu, T
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 3 (2003)
Publisher : Indonesian Animal Sciences Society

Feed additives are commonly added in poultry feed as a growth promotant or to improve feed efficiency. The most common feed additive used is antibiotic at sub-therapheutic doses, although there is a controversy on its impact on human health. Previous results showed that Aloe vera gel could improve feed efficiency in broilers and an in vitro study showed that the extract have an antibacterial effect. Therefore, a further experiment was designed to study the response of broilers to Aloe vera gel or its extract as feed additives. Aloe vera was prepared in dry gel or chloroform-extract and included in the diet at levels of 0.25; 0.50 and 1.00 g/kg (equal to dry gel). Standard diets with or without antibiotic were also formulated as control and a commercial diet was included for comparison. The diets were fed to broilers from day old to 5 weeks. Each treatment has 9 replicates and 6chicks/replicate. Parameters observed were: feed consumption, weight gain and feed convertion ratios. Carcass yield, abdominal fat levels, relative weight of liver, gizzard, tractus digestivus and length of tractus digestivus were also measured at the end of feeding trial. The results showed that Aloe gel and its extract did not influence body weight gain and feed consumption of broilers significantly (P>0.05), but improved feed convertion slightly (3.50%). The response in this trial was similar as thosecommercial diet and diet added with antibiotic. There was no significant (P>0.05) effect of Aloe vera bioactives on carcass yield, abdominal fat level and relative weight of liver. However, Aloe vera gel and its extract tend to increase gizzard weight, gastro intestinal weight and length. The Aloe vera gel and its extract also reduced the total count of aerobic bacteria in the digesta of tractus digestivus. It is concluded that the Aloe vera gel improve feed efficiency in broilers by increasing the size of tractus digestivus and reducing the total count of aerobic bacteria in the gastro intestinal tract. Â Key words: Aloe vera, feed additives, broilers, anti-bacteria

Nutritive value of fermented palm oil sludge added with different sources of phosphorus Pasaribu, Tiurma; Arini, N; Purwadaria, T; Sinurat, A.P
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 3 (2003)
Publisher : Indonesian Animal Sciences Society

The experiment has been conducted to determine the nutritive value of palm oil sludge fermented with A. niger added with different sources of phosphorus (NPK, P2O5, NaH2PO4). The experiment was assigned in a factorial (3x3) design. The main factor was sources of phosphorus, while the sub factor was time of incubation (0, 4 days aerobic incubation, and 4 days aerobic incubation followed by 2 days anaerobic incubation). Parameters measured were pH, soluble nitrogen, true protein and crude protein, total Î±-amino acid (TAAA), soluble phosphorus and total phosphor, in vitro dry matter (DCBK), and true protein digestibilities (DCP). Results from the analyses showed that fermentation increased the contents of soluble and total P, protein and TAAA and the value of in vitro protein digestibility. Fermented product added with P2O5 had the highest in vitro dry matter digestibility, while the one added with NPK had true protein content and digestibility. It was concluded that additional NPK gave the best result interm of nutritive value. Â Key words: Palm oil sludge, A. niger, phosphorus sources

Performances of broiler supplemented with antibiotic and Morinda citrifolia waste as sources of bioactive compounds Bintang, I.A.K; Sinurat, A.P; Purwadaria, T
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

A study on the use of Zinc bacitracin antibiotic (ZnB) and dried Morinda citrifolia waste as feed additive in broiler ration was conducted. Two hundred and sixteen day old chicks (DOC) was allocated into 4 treatments: control; control + 50 ppm ZnB, and control + M. citrifolia waste at 2 levels (5 and 10 g/kg ration) with 9 replications. Each replicatation consist of 6 birds. The treatments were allocated in a completely randomized design. Variables measured were feed intake, live weight, feed conversion ratio (FCR), percentages of carcass and internal organs (liver, gizard, abdominal fat, and thickness of intestine). The results showed that feed intake ofÂ M. citrifolia waste was significantly (P<0.05) lower than that of control. Live weight and live weight gain of M. citrifolia waste treatments did not significantly different (P>0.05) from that of control. The use of M. citrifolia waste (5 and 10 g/kg) gave significantly lower (P<0.05) live weight than the use of antibiotic. FCR of M. citrifolia waste 5 g/kg ration and antibiotic was not significantly different (P>0.05), but was significantly lower (P<0.05 ) than that of control and M. citrifolia waste 10 g/kg. Carcass and internal organ percentages were not effected by antibiotic and M. citrifolia waste. Liver percentages with M. citrifolia waste 10 g/kg was significantly (P<0.05) higher and abdominal fat was lower (P>0,05) than that of control, control + antibiotic ZnB and M. citrifolia waste 5g/kg. It is concluded thatÂ the supplementation of M. citrifolia waste (5 g/kg) could substitute ZnB to improve feed eficiency in broiler ration. Key Words: M. Citrifolia Waste, Performances, Broiler

The estimation of genetic distance and discriminant variables on breed of Indonesian sheep by morphological analysis Suparyanto, Agus; Purwadaria, T; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

The morphological discriminant and canonical analyses were carried out to determine the genetic distance and discriminant variables between Indonesian breeds of thin tail sheep from Deli Serdang (North Sumatera), Ciamis and Garut (West Java) and fat tail sheep from Garahan and Pamekasan (East Java). The number of sheep randomly collected from farmers was 665 heads, while that from breeding station was 183 heads. Zoometrical variables studied were body weight, body length, wither height, chest depth, chest circumference, hip height, hip depth, hip circumference, tail length, tail width and tail thickness. SAS package program was used to analyze the data. Results from distribution mapping produced by canonical analysis and Mahalanobis distance (phenogram tree) showed that breeds are divided into two groups. The first group is thin tail sheep which consists of Ciamis, Garut and Sumatera, and the other is fat tail sheep which consists of Garahan and Pamekasan. The groups of Ciami and Garut were mixed by Garahan with 1.01 and 1.20%, respectively but were not contaminated by Pamekasan (0.00%). The highest similarity between individual inside the group was obtained from Sumatera (84.27%) due to the isolated flock. It was mixed more by Ciamis (7.87%) and Garut (5.62%) than Garahan (1.69%) and Pamekasan (0.50%). Results from canonical analysis also showed that body length, chest circumference, hip circumference, and body weight was less discriminant variables to determine the differences between breeds, while the most discriminant variables were obtained by tail length, tail width and tailthickness. Â Key words : Sheep, genetic distance, morphology

The changes of peroxide number of coconut meal during storage and fermentation processed with Aspergillus nige Hamid, Helmi; Purwadaria, T; Haryati, T; Ketaren, A.P
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

The effect of fermentation process and duration of storage of fermented coconut meal with Aspergillus niger on its peroxide number has been studied. First stage of experiment was arranged in a completely randomized design with four treatments (raw coconut meal, 0 and 4 day aerobic fermentation incubation and after 2 day anaerobic enzymatic incubation). The peroxide number was determined as potentiality for rancidity. The three incubation processes decreased the peroxide number significantly. The highest reduction was obtained at 4 day aerobic fermentation (49.7%). The decrease of peroxide number of fermented coconut meal was correlated with the decrease of lipid content. The regression coefficient is highly significant (r2 = 0.76, P<0.01). In the second experiment, the effect of storage on the peroxide number of fermented product was conducted by factorial design 2x3x4 i.e., two type of substrates (fermented and non fermented coconut meal), 3 storage temperatures (-13, 4, and 29oC) and 4 storage times (1, 2, 3, and 4 months). Statistical analysis for lipid contents showed that there was significant interaction (P<0.05) between kind of substrates and storage time. The lipid content of fermented product was decreasing from the first up to the third month of storage, then it was increasing at the fourth month, but the lipid content of non fermented coconut meal did not significantly changed (P>0.05). Statistical analysis for the peroxide number showed that there was highly significant interaction between the type of substrates and storage temperature. The peroxide number on the fermented products did not significantly increase, where as the non fermented products show significantly increased during storage course. The significant interaction between storage duration and temperature observed. At low temperatures (-13 and 4Â°C) storage the peroxide number increased 44%, while at high temperature (29Â°C) up to 95%. The highest peroxide number on the fermented product (43.5 ppm) was obtained at the temperature storage of 29Â°C for 4 month period. This value is still under rancidity limit (80 ppm). It could be concluded that fermentation process reduced the rancidity of coconut meal. Â Key words : Coconut meal, fermentation, peroxide number, Aspergillus niger

Fermented and unfermented palm kernel cake as broiler chicken Ketaren, P.P; Sinurat, A.P; Zainuddin, D; Purwadaria, T; Kompiang, I.P
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

feed. Two hundred and ten, day-old broiler chicks were used for this study. They were allotted to 6 different diets containing either BIS or FBIS at 3 different levels (5, 10 and 15%) and one control diet. The results showed that 5% BIS and 5% FBIS could be used in broiler diet without adversely affecting feed intake, weight gain and feed conversion ratio. FCR of those diets were significantly (P<0.05) better than the control diet. Carcass yields were not significantly affected by feeding of BIS nor FBIS. The FBIS diet produced less abdominal fat than the BIS diet. Â Key words : Palm kernel cake, fermentation, broilers

kernel meal and its fermented product for growing ducks Bintang, I.A.K; Sinurat, A.P; Murtisari, T; Pasaribu, T; Purwadaria, T; Haryati, T
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 3 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v4i3.156

A series of experiment was carried out to evaluate the use of palm kernel meal and its fermented product in the ration of growing ducks. Treatments were arranged in a 2x3 factorial experiment, i.e. the combination between two processes (unfermented and fermented palm kernel meal) and three levels of ingredients in the diet (5, 10 and 15%). Four replicates were applied for each treatment, with 8 ducklings in each replicate. A control diet with no palm kernel meal was also formulated. All diets were formulated to have a similar protein and ME contents. Results of the experiment showed that fermentation process increased the nutrient composition (crude protein, true protein, phosphorus, and the metabolizable energy) and reduced its fibre and fat content. The feeding trial showed that neither the fermentation process, levels of ingredients nor the interactions of the two factors affected final body weight, feed consumption, feed conversion, carcass percentage and abdominal fat of the ducklings significantly. It is concluded that fermentation process improved the nutritional value of palm kernel meal and the inclusion of palm kernel meal (unfermented and fermented), up to 15% in the diet of ducks did not negatively affect the growthperformance and the carcass yield. Â Key words : Ducks, palm kernel meal, fermentation

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