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ASPERGILLUS GLAUCUS GROUP DAN PENICILLIUM SP DI RUANG OPERASI BEDAH SARAF Nurul Hasanah; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1100

Abstract

Nosocomial infections occur widely in the world, most of them were in the poor and developing countries, because those infectiondiseases were still the mayor cause of high morbidity and mortality. All microorganisms including fungi may cause nosocomial infection.The fungal as opportunistic pathogens can threat immunocompromised patients such as neurosurgical patients and HIV/AIDS patients.The aim of this study was to identify the fungal species found in the neurosurgery and HIV/AIDS rooms at Dr. Wahidin SudirohusodoHospital Makassar. This study was a cross sectional study. The sample was the air in neurosurgery operating theater and HIV/AIDSward collected using Micro biology Air Sampler 100. The identification of fungal species using lacto phenol cotton blue stain were done inBalai Besar Laboratorium Kesehatan Makassar in the period of June up to July 2010. The amount of fungal colonies in the neurosurgeryroom was 36 CFU/m3 and the identified fungi were Aspergillus’s glaucus group and Penicillum sp. The amount range of fungal coloniesin HIV/AIDS ward were 102–158 CFU/m3 and the identified fungi were: Aspergillus’s Niger, Aspergillus’s glaucus group and Penicilliumsp. Based on this study it can be concluded that only Aspergillus’s glaucus and Penicillium sp were found in the neurosurgery operatingtheater and HIV/AIDS ward, while Aspergillus’s Niger was only found in the HIV/AIDS ward.
BAKTERI AEROB DAN UJI KEPEKAAN ANTIMIKROBA Erviani Zuhriah; Nurhayana Sennang; Darmawaty ER
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.385

Abstract

Nosocomial infection is an infection acquired in hospitals, which occur more frequently in poor and developing countries such as Indonesia, one percent of the related cases leads to death. The occurrence of nosocomial infection causes the lengthening of hospitalization and increased risk of disease transmission. According to Permenkes No. 1204/Menkes/SK/X/2004, the Emergency Room is one of the high-risk areas. The objectives of this research were to quantify the number of aerobic bacteria, to know the identification method of the pathogenic bacteria and to determine the antimicrobial sensitivity pattern in the emergency installation. A cross sectional study was performed by collecting air samples at eleven treatment rooms of the Emergency installation in dr. Wahidin Sudirohusodo Hospital Makassar by using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and antimicrobial susceptibility tests (AST) were conducted at the Balai Besar Laboratorium Kesehatan Makassar (July to September 2009). The number of bacterial colonies were 288–6570 CFU/m3. Pathogenic bacteria identified in the study were Stomatococcus mucilaginous, Staphylococcus haemolyticus and Acinetobacter calcoaceticus, which were still sensitive to tetracycline and resistant to ceftazidim and kanamycin.The number of bacterial colonies exceeded the standard number established by the Decree of Indonesian Health Minister
BAKTERI AEROB PATOGEN DAN UJI KEPEKAAN ANTIMIKROBA DI RUANGAN PERAWATAN PENYAKIT DALAM Fedelia Raya; Nurhayana Sennang; Suci Aprianti
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.374

Abstract

Pathogenic bacteria are the major causes of airborne infection at the hospital ward. Nosocomial infection can occur at the opened as well as at the closed room. Nosocomial infection influences the morbidity and mortality in the hospital and need an extra attention, because of the increased number of hospital patients, micro organism mutation and increased of bacteria resistance to antibiotics. The aim of this study was to quantify the number of aerobic bacteria, and to know the pathogenic bacteria identification and its determination on the susceptibility of the antimicrobial problems at the internal medicine ward. This research was carried by a cross sectional study, which performed by collecting air samples in eight internal medicine ward of Dr. Wahidin Sudirohusodo Hospital using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and the antimicrobial susceptibility test (AST) were conducted at the Balai Besar Laboratorium Kesehatan (July to August 2009). In this study were found the numbers of bacteria colonies about 580–6040 CFU/m3. The pathogenic bacteria that identified were Acinetobacter calcoaceticus, Staphylococcus saprohpyticus, Enterobacter hafniae and Stomatococcus mucilaginosus that were sensitive to Amikasin, Gentamicyn, Azitromycin and Norfloxacyn but resistant to Ampicillin. The number of bacterial colonies exceeded the established number standard by Decree of the Indonesian Health Minister. The pathogenic bacteria showed the most sensitive result of AST were Acinetobacter calcoaceticus, Enterobacter hafniae, Stomatococcus mucilaginosus and Staphylococcus saprohpyticus.
BAKTERI PATOGEN AEROB DAN UJI KEPEKAANNYA DI RUANGAN BEDAH PUSAT Agustini Agustini; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1073

Abstract

An operating room is potential in causing nosocomial infection in a hospital, especially due to surgical wound infections. Nosocomialinfection raises the length of day care and disease transmission risk. According to Permenkes No. 1204/Menkes/SK/X/2004, the operatingroom is a highly infection risk area. The aim of this study is to know the determination of the amount of bacterial colonies count, andthe sensitivity identification pattern of pathogenic bacteria in the central operating rooms at the Dr Wahidin Sudirohusodo Hospital.A cross sectional study was done by taking air samples using a Microbiology Air Sampler 100 in six central operating rooms in Dr.Wahidin Sudirohusodo Hospital. Counting bacterial colonies, identifying pathogenic bacteria and sensitivity test were done at the BalaiBesar Laboratorium Kesehatan Makassar (from May to June 2010). The result of the study shows that the amount of bacterial coloniesfound were approximately 148–440 CFU/m3 aerobic pathogenic bacteria consisting of Alcaligenes faecalis and Serratia liquefacienswhich were sensitive to sulbactam+ cefoperazone, gentamycin, levofloxacin but resistant to ampicillin, nitrofurantoin, clindamycin andmetronidazole. The researchers concluded that the amount of bacterial colonies found exceeded the Permenkes standard. The pathogenicbacteria were sensitive to sulbactam+ cefoperazone, gentamycin and levofloxacin however, resistant to ampicillin, nitrofurantoin,clindamycin, and metronidazole.