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Struktur Komunitas Semut (Hymenoptera : Formicidae) di Lahan Pertanian Organik dan Anorganik Desa Batur, Kecamatan Getasan, Kabupaten Semarang Putra, Ivan Mahadika; Hadi, Mochamad; Rahadian, Rully
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.659 KB) | DOI: 10.14710/bioma.19.2.170-176

Abstract

Ants play an important role in terrestrial ecosystems, including in organic and inorganic farmland, as predators, scavengers, and their interaction with other organism. The objectives of this study were to compare the community structure and the role of the ant in organic and inorganic farmland, and also to determine soil abiotic factors in both farmland. The study was conducted in organic and inorganic chili crop land at Batur village, Getasan, Semarang. The sampling of ants was performed using pitfall traps and bait traps, and then the ants were identified. Data analysis used is an abundance index, diversity index, evenness index, Hutchinson test and similarity index. The results showed that the number of species and the number of individual of ants in organic farm were higher (45 individuals, 8 species) than inorganic farm (35 individuals, 6 species). Diversity index of ant species in both farmlands were categorized moderate with the index value of 1.92 and 1.42. Statistically, there were no significant differences in species diversity of ants in organic and inorganic farmland. There were five dominant species in organic field and three dominant species in inorganic field. Ants that predominate in both farmlands were Iridomyrmexsp, Tetramorium sp, Solenopsisgeminata, Odontoponeradenticulata and Diacammasp. The similarity of ant communities between organic and inorganic farmland showed high value of similarity index (IS) which was 85.7%. Statistically, abiotic factors showed that there were significantly different of some abiotic factors in both farmlands, the content of carbon, phospor, organic matter and c / n ratio. Keywords: community structure of ants, agricultural ecosystems
Pengaruh Biostarter Pengurai Bahan Organik Terhadap Kapasitas Infiltrasi Air dan Struktur Komunitas Mesofauna Tanah Andriani, Latifah Fitria; Rahadian, Rully
JURNAL SAINS DAN MATEMATIKA Volume 20 Issue 1 Year 2012
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4903.307 KB)

Abstract

Peran mikroorganisme dan fauna tanah dalam menentukan kualitas tanah telah diketahui cukup luas, namun perannya terhadap kapasitas infiltrasi air belum banyak dikaji. Penelitian ini secara umum bertujuan untuk mengetahui pengaruh biostarter berupa inokulan mikroba komersial untuk perbaikan kualitas tanah. Secara khusus penelitian ini bertujuan untuk mengetahui pengaruh produk biostarter terhadap struktur komunitas mesofauna tanah dan laju infiltrasi air serta korelasi antara struktur komunitas mesofauna tanah dengan kapasitas infiltrasi. Penelitian ini menggunakan Rancangan Acak Lengkap faktor tunggal dengan empat perlakuan biostarter yaitu EM4, Bioklin, Mig Dec, dan Orgadec. Periode sampling dilakukan dua kali pada hari ke 30 dan hari ke 50 inkubasi. Hasil penelitian menunjukkan bahwa penambahan biostarter memengaruhi struktur komunitas mesofauna tanah, khususnya berpengaruh positif terhadap kelimpahan Oribatida dan Mesostigmata. Produk Orgadec secara konsisten mampu meningkatkan kelimpahan mesofauna tanah paling tinggi pada  30 hari maupun 50 hari. Laju infiltrasi pada umur inkubasi 30 hari menunjukkan adanya perbedaan yang nyata pada perlakuan Orgadec saja, sedangkan pada umur 50 hari, perbedaan secara nyata ditunjukkan pada perlakuan EM4, Mig Dec dan Orgadec. Secara statistik, total kelimpahan mesofauna tanah dan kelimpahan Oribatida menunjukkan perbedaan yang nyata dengan laju infiltrasi.   Keywords: biostarter, struktur komunitas, mesofauna tanah, laju infiltrasi
Kelimpahan Dan Keragaman Serangga OPT (Organisme Pengganggu Tanaman) dan Musuh Alaminya pada Tanaman Jagung dan Padi dengan Sistem Rotasi Tanaman Ervianna, Ana Rita; Hadi, Mochamad; Rahadian, Rully
Bioma : Berkala Ilmiah Biologi Vol. 22, No 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.997 KB) | DOI: 10.14710/bioma.22.1.59-69

Abstract

Insects in agricultural ecosystem can play a role as pests or natural enemies. The abundance and diversity of insect pests and natural enemies are interconnected because insect pests are food for natural enemy insects. The aims of the study were to compare the abundance and diversity of pest insects and natural enemies of corn and rice plant, and to determine the similarity of pest insects and natural enemies of corn and rice plant using crop rotation systems. The sampling has performed four times i.e, in the vegetative phase of corn, generative corn, vegetative rice, and generative rice. The sampling method used sweep net method. The results showed that the number of individual pest insects and natural enemies of each growth phase of corn plants were lower than rice plants. The number of species pest insect and natural enemies of each growth phase of corn plants were lower than rice plants. In addition the diversity of pest insect of each growth phase of corn plants was higher than rice plants while the diversity of natural enemies in each growth phase of corn plants was lower than rice plants. The diversity index of pest insects and natural enemies of each growth phase in corn plants and rice plants was in the medium category. The presence of pest insects and  natural enemies in each growth phase of corn and rice plants shows the species in the two compared ecosystems were different.
THE TOOL OF RESISTANCE MONITORING OF PLUTELLA XYLOSTELLA, (L.) AND ITS FIELD VALIDATION USING EMAMECTIN BENZOATE AND DIAGNOSTIC CONCENTRATION DETERMINATION Tarwotjo, Udi; Situmorang, Jesmandt; Martono, Edhy; Rahadian, Rully
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.1.50-54

Abstract

In Indonesia, cabbage pest control is still heavily dependent on the use of insecticides. Although insecticides have many advantages, i.e., reducing the populations of pest quickly, easy to use, and economically advantageous, but it has the negative impact especially the emergence of resistant pest species.The objective of this study are: (1) to monitore the resistance of Plutella xylostella population toward insecticide residues of emamectin benzoate by diagnostic concentration determination, (2) to validate the diagnostic concentration in the field. P. xylostella population were collected from some cabbage farms in Central Java i.e., Bandungan, Sumowono, Cepogo, Kejajar, Kertek, Tawangmangu, Plalar, Puasan, Kaponan, and Keteb.Determination of LC90 was tested using probit analysis. The results showed that five of the eleven tested populations have sensitivity c2 value < c2 table, namely population Selo, Gedongsongo, Gondosuli, Kejajar, and Plalar. The determinated diagnostic concentration is LC90 = 2443.99 ppb with the lowest concentration limits 1213.67 ppb and the highest is 4921.53 ppb, because of the testing results of the sensitivity of all tested populations are still susceptible to emamectin benzoate with mortality > 90% at a concentration of 2000 ppb. The results of validation diagnostic concentration (2443.99 ppb), indicating the mortality percentage caused by eleventh population ranged from 82.76% to 95%, the value of c2 value is smaller than c2 table (df = 1; ? = 0.05) = 3.84. Therefore the diagnostic concentration (ppb 2443.99) is valid for effective monitoring tool for the development of resistance of P. xylostella population. The conventional method to monitor resistance is less sensitive to the resistance emerging occurrence. Therefore, it is necessary to develop monitoring methods which are more sensitive to small changes in the frequency of resistance, by using diagnostic concentration.
Keanekaragaman dan Kelimpahan Jenis Burung di Pusat Restorasi Mangrove Mojo Kabupaten Pemalang Tamar, Irsyad Muhammad; Baskoro, Karyadi; Hadi, Mochamad; Rahadian, Rully
Bioma : Berkala Ilmiah Biologi Vol. 22, No 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.22.2.121-129

Abstract

Mojo Mangrove is a forest area located in the tidal area and becomes a habitat for various organisms in it. Mojo mangrove forest is an ecosystem that is a place to live and breed various species of birds, because of the existence of habitat and food that supports. The location of the Mojo mangrove forest which is far from the settlement is a supporting factor which is the reason birds love the habitat. Species diversity and abundance of bird species can reflect the high biodiversity of other wildlife, meaning birds can be used as indicators of forest quality. The purpose of this study was to determine the diversity and abundance of bird species, and determine the status of bird conservation in the Mojo Pemalang Mangrove Restoration Center. The study was conducted from February to April 2019 at the Mojo Mangrove Restoration Center in Pemalang District, using the point count method. The analysis carried out was a species abundance index, a species diversity index, a species evenness index, a similarity index, and the Hutchinson T test. There are 53 species of 27 families. The diversity of bird species is included in the medium category with an index of 1.97 - 2.85. Based on IUCN, there are 49 species of birds included in the Least concern category, 2 species included in the vulnurable that is Javan coucal (Centropus nigrorufus) and Javan myna (Acridotheres javanicus), 2 species included in the Near threatned category that is Oriental darter (Anhinga melanogaster) dan Javan plover (Charadrius javanicus). Based on Law No. 5 In 1990 there were 10 protected bird species.
Resistance Monitoring of Nilaparvata lugens Stall against Pymetrozine Insecticide with Determination of Diagnostic Concentrations Murtiati, Sri; Tarwotjo, Udi; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28237

Abstract

Nilaparvata lugens Stall is one of the main insect pests on rice crops. Intensive control of this pests using insecticides has resulted in the development of insect resistance. This study aimed to find out the level of resistance of the N. lugens population to pymetrozine insecticides by determining the diagnostic concertations. N. lugens was collected from five endemic areas in Central Java Province from October 2019 to June 2020. The data from the bioassay test were analyzed with probit analysis to obtain the LC50 value. The results of the sensitivity test showed that the Kajen population has the highest RF value (2.47), while the Karanganyar population which has the lowest RF value (1) was the most sensitive population. The determined diagnostic concentration was LC95 = 25.52 ppm with the lowest concentration limit of 7.67 ppm and the highest of 30.05 ppm. To conclude, the determined diagnostic concentration (LC95 = 25.52 ppm) is effective for detecting the susceptibililty of N. lugens population. This finding would be beneficial for monitoring resistance of N. lugens population against pymetrozine insecticides in the field.
Kelimpahan dan Keanekaragaman Serangga OPT Nokturnal beserta Musuh Alaminya pada berbagai Macam Tanaman Sayur di Lahan Organik, Desa Batur, Kabupaten Semarang Faiz, Mohamad; Hadi, Mochamad; Rahadian, Rully
NICHE Journal of Tropical Biology Vol 7, No 1 (2024): NICHE Journal of Tropical Biology Volume 7, Number 1, May 2024
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.7.1.1-10

Abstract

Utilizing Environmental DNA to Identify Eukaryotic Diversity in Mangrove Sediments at Demak, Central Java, Indonesia Cahyani, Ni Kadek Dita; Jumari, Jumari; Hariyati, Riche; Rahadian, Rully; Rahim, Aulia; Zega, Enita Setiawati; Kholifah, Rizqi Widya Nur; Barber, Paul Hendry; Putro, Sapto Purnomo; Soeprobowati, Tri Retnaningsih
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 4 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.4.543-556

Abstract

The mangrove ecosystem, found along tropical and subtropical coasts, adapts to extreme conditions like rapid tidal changes, high salinity, anthropogenic influences, and anoxic environments. Mangrove sediments host diverse organisms, particularly invertebrates and bacteria, which significantly influence sediment structure and biochemical processes by enhancing permeability and water flow. Modern molecular approaches, notably Next-Generation Sequencing (NGS), are increasingly used to identify macro and microorganism communities in sediments. NGS, a powerful tool for DNA and RNA sequencing, allows for parallel sequencing of numerous DNA fragments, providing comprehensive insights into genome structure, genetic variants, gene expression, and epigenetic modifications. Its efficiency and cost-effectiveness make NGS vital for both basic biological research and clinical diagnostics. Recent NGS studies on mangrove sediments have focused on bacterial, archaeal, and fungal diversity. The study examines eukaryotic diversity in mangrove sediments at two locations, targeting the Cytochrome Oxidase Subunit I (COI) gene, a universal marker for eukaryotes. Results indicate distinct taxa at each site with minimal overlap, demonstrating eDNA's potential for assessing both macro and microorganism diversity in mangrove sediments. This preliminary study underscores the utility of molecular techniques in biodiversity research and also dynamic ecosystem changes in the mangrove sediment ecosystem. The high influence of the environment around the mangrove ecosystem will affect the quality of the mangrove itself. eDNA here provides a fast method for recording possible changes to be able to carry out better management in the future.
Soil Fertility Analysis using Microarthropod Diversity Index in Coffee-Based Agroforestry in Mount Ungaran, Indonesia Ari, Psn Masruri Sulistiyanto; Alkian, Ilham; Rahadian, Rully
BUMI: International Journal of Environmental Reviews Vol. 1 No. 02 (2023): BUMI: International Journal of Environmental Reviews
Publisher : UIN Sulthan Thaha Saifuddin Jambi, Pusat Kajian Lingungan Hidup

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/bumi.v1i02.2223

Abstract

The conversion of forest functions to plantation land results in decreased land function and ecosystem disruption. Coffee-based agroforestry is a management system that is considered capable of maintaining land functions and reducing the level of leaching of nutrients. This study uses the soil microarthropod diversity index to evaluate the soil fertility level in 2 types of coffee-based agroforestry in Mount Ungaran. Sampling was conducted in Banyuwindu to represent intensive land and Gonoharjo as non-intensive land. Microarthropod samples in the soil were analyzed using Pitfall traps and extracted using the Tullgren-Funnel method. Soil microarthropods from the sampling were identified in family-level taxa. The species diversity index was analyzed using the Hutcheson t-test and the Sorensen similarity index. Characteristics of organic composition showed that intensive land had a value of 17% lower than non-intensive land. Environmental characteristics of intensive land types tend to have lower pH and soil moisture values ​​than non-intensive land. The Shanon-Wiener diversity index analysis showed that soil microarthropods on the Intensive land type were H'= 2.08 and the Non-Intensive was H'= 2.30. Hutcheson's t-test showed that the diversity of soil microarthropods was significantly different with a significant value (p-value < 0.05), and the Sorensen similarity index value showed a similarity level of 52.17%. Based on the results of various analyses, it can be concluded that the intensive type of land has lower soil fertility than non-intensive land in the coffee agroforestry system in Mount Ungaran.
Keanekaragaman Serangga Asosiatif pada Bangkai Tikus yang Diletakkan di Luar Ruangan sebagai Indikator Forensik Aini, Aprilia Nurul; Rahadian, Rully; Khotimah, Khusnul
Biocaster : Jurnal Kajian Biologi Vol. 5 No. 3 (2025): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v5i3.502

Abstract

Insects are a group of organisms with a high level of species diversity and have various important roles, one of which is in the field of forensics. Insects can be used as forensic indicators in revealing the facts of death studied in forensic entomology. This study aims to analyze the diversity of insects associated with rat (Rattus norvegicus) carcasses in three different conditions, namely without treatment (control), wrapped in cloth, and with incisions. This study used a Randomized Block Design (RBD) with three replications for each treatment. The treatment group consisted of three types, namely without treatment, with cloth wrapping, and with incisions. Each group consisted of three replications. The results of the Hutcheson t-test showed differences in the insect species diversity index between treatments (p < 0.05). The species Onthophagus pilularius and Phaeochrous emarginatus were only found in carcasses wrapped in cloth. Meanwhile, Chrysomya megacephala was the dominant species in all treatments with a relative abundance of more than 40%. These findings indicate that the physical condition of the carcass influences the composition of the insect community, which has the potential to be used as an indicator in forensic studies.