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All Journal Journal of Parasite Science Journal of Applied Veterinary Science and Technology
Mohammad Sukmanadi
Departemen Kedokteran Hewan Dasar Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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The Anthelmintics Activity of Ethanol Extract of African Leaf (Vernonia amygdalina) on Mortality of Fasciola gigantica in Vitro Dhio Asmaydo; Iwan Sahrial Hamid; Kusnoto Kusnoto; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.027 KB) | DOI: 10.20473/jops.v3i1.16426

Abstract

The Aims of this study is to know the anthelmintics activity of ethanol extract of african leaf (Vernonia amygdalina) on mortality of Fasciola gigantica in Vitro. Method that used in the research was a completely randomized design. There were five treatments and each treatment was done in four replications. This research used ten Fasciola gigantica in each treatment for all replications. The observation and recording of dead Fasciola gigantica was done at 2, 4, 6, 8, and 10 hours.  Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (500C). The obtained data was analyzed using ANOVA and continued with Duncan Multiple Range Test. The result of this research show that ethanol of extract of African Leaf (Vernonia amygdalina) has activity anthelmintic on mortality of Fasciola gigantica in Vitro. Ethanol extract african leaf (Vernonia amygdalina) with concentration of 1.4% is the optimal concentration of anthelmintics. This higher the concentration of the extract, the higher the property of anthelmintics.
The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro Meta Aprilia; Poedji Hastutiek; Rochmah Kurnijasanti; Lucia Tri Suwanti; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.694 KB) | DOI: 10.20473/jops.v3i1.16429

Abstract

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.
Identifikasi Cacing Nematoda pada Sekum dan Kolon Sapi Kurban yang Dipotong saat Idul Adha 1439 H di Wilayah Surabaya Timur Jihaan Haajidah; Moh Sukmanadi; Kusnoto Kusnoto; Endang Suprihati; Lianny Nangoi; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.551 KB) | DOI: 10.20473/jops.v4i1.20272

Abstract

The aim of this research was to detect the presence of nematode worms that infects sacrificial cattle slaughtered duringEid al-Adha 1439 H in East Surabaya. This research used 24 sampels of sacrificial cattle and used digestive tract surgical method. Based on the result of the examination using a microscope with 100x magnification, there are positive samples infected with nematode worms. In caecum cattle consist of Oesophagostomum radiatum and Trichuris spp. In colon cattle consist of Oesophagostomum radiatum.
Identifikasi Parasit Pentastomida pada Biawak Air (Varanus salvator) yang akan dikonsumsi Andhika Yudhantama Subroto; I Komang Wiarsa Sardjana; Moh Sukmanadi; E Djoko Poetranto; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.512 KB) | DOI: 10.20473/jops.v4i1.20276

Abstract

This study aims to determine the pentastomide parasite that infects the digestive tract of giant water lizards examined in Laboratory of Parasitology Department, Faculty of Veterinary Medicine, Airlangga University. In this study, three sample were found to be infected with pentastomide parasite in the lung. The phase found was adult phase pentastomide; it has pentastomide spermatheca which shows that this parasite is female. 
The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro Dwi Retno Widyawati; Iwan Sahrial Hamid; Moh. Sukmanadi; Sri Agus Sudjarwo; Muhammad Hambal
Journal of Parasite Science (JoPS) Vol. 5 No. 1 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.613 KB) | DOI: 10.20473/jops.v5i1.29959

Abstract

The purpose of this research is to find the effectiveness of bandotan’s (Ageratum conyzoides L.) leaf extract on mortality of Boophilus microplus in vitro and also to get of lethal concentration and lethal time. This research used 125 Boophilus microplus. This research used five treatments, namely neguvon suspensionon (K+), ethanol 1% (K-), 1% concentration (P1), 2% concentration (P2) and 3% concentration (P3). The observation of mortality was done every six hours. Boophilus microplus dies when there is no movement. The data were analyzed using one-way ANOVA and followed by Duncan's Multiple Distance Test. The result is said to be significant if p<0.05. Probit analysis of LC50 is 1.63% at the first hour, 0.85% at the second hour, 0.54% at the third hour, 0.34% at the fourth hour, 0.04% at the fifth hour and 0.04% at the sixth hour, LC90 reached by using 5.6%, 2.9%, 1.8%, 1.2%, 0.16 and 0.16% concentration in 1, 2, 3, 4, 5 and 6 hours, respectively. The LT50 of ethanol 1% (K-) was at 10.142 hours, neguvon suspension on (K+) was at 0.747 hours, 1% concentration (P1) was at 2.184 hours, 2% concentration (P2) was at 1.579 hours, 3% concentration (P3) was at 0.923 hours and LT90 was at 23.734, 1.749, 5.110, 3.695 and 2.159 hours. The conclusion of this study was bandotan’s (Ageratum conyzoides L.) leaf extract was effective as an acaricide based on the mortality of Boophilus microplus.
Nephroprotective Effect of Dayak Onion (Eleutherine palmifolia) Against Monosodium Glutamate-Induced Renal Toxicity in Mice (Mus musculus) Anggreani Desi Ramadhani Rahajeng; Aldin Akbar Rahmatullah; Cindy Ercha Aulia Putri; Rahmi Sugihartuti; Endang Suprihati; Hani Plumeriastuti; Mohammad Sukmanadi; Iwan Sahrial Hamid
Journal of Applied Veterinary Science And Technology Vol. 5 No. 2 (2024): October 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I2.2024.129-134

Abstract

Background: The toxicological impacts of monosodium glutamate (MSG), a commonly used food additive, present a significant public health concern. Excessive MSG consumption will increase radical oxidative species, inducing a stress oxidative condition that ultimately results in kidney damage. Purpose: This study aimed to explore the nephroprotective effects of ethanol extracts from Dayak onion (Eleutherine palmifolia) against MSG-induced renal toxicity in mice (Mus musculus). Method: Twenty-five 11 week-old male mice, weighing approximately 20 g each, were divided into five research groups. Group C- received only CMC-Na 0.5%, Group C+ received 4000 mg/kg BW MSG, while Groups T1, T2, and T3 were administered 4000 mg/kg BW MSG along with Dayak onion extract at doses of 30 mg/kg BW, 60 mg/kg BW, and 120 mg/kg BW respectively. All treatments were carried out orally for 52 days. The mice were euthanized by cervical dislocation, and their kidneys were extracted for the examination of any histopathological changes. The data underwent analysis utilizing the Kruskal-Wallis test, followed by the Mann-Whitney test. Results: The results as indicated by the histopathological evaluation of the mice’s kidneys revealed significant improvements in the histo-architecture of the kidneys. Supplementation of Dayak onion extract in mice induced with MSG decreased the degeneration and necrosis of the tubule epithelium, and it also repaired the glomerular necrosis. Conclusion: Oral administration of Dayak onion extract to mice exposed to monosodium glutamate demonstrated a notable reduction in kidney damage and helped maintain renal health significantly.
Co-Authors Aldin Akbar Rahmatullah Andhika Yudhantama Subroto Anggreani Desi Ramadhani Rahajeng Cindy Ercha Aulia Putri Dhio Asmaydo Dwi Retno Widyawati E Djoko Poetranto Endang Suprihati Hani Plumeriastuti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Kusnoto Kusnoto Lianny Nangoi Lucia Tri Suwanti, Lucia Tri Meta Aprilia Muhammad Hambal Poedji Hastutiek Rahmi Sugihartuti Rochmah Kurnijasanti Sardjana, I Komang Wiarsa Sunarso, Agus