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All Journal Jurnal Riset Teknologi Pencegahan Pencemaran Industri
Silvy Djayanti
Balai Besar Teknologi Pencegahan Pencemaran Industri
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Environmental Science

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Low Energy Bacteria Preservation of Extremely Halophilic Archaea Haloferax Lucentense and Haloferax Chudinovii Immobilized using Natural Zeolite Rizal Awaludin Malik; Nilawati Nilawati; Novarina Irnaning Handayani; Rame Rame; Silvy Djayanti; Ningsih Ika Pratiwi; Nanik Indah Setianingsih; Nasuka Nasuka
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 2 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no2.p16-28

Abstract

The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research was the type of the carrier which was raw zeolite, 110oC and 300oC heat-activated zeolite carrier, parameters measured in this study was physical and chemical of zeolite such as chemical content, Si/Al ratio, surface area and pore volume, and biochemical assay, bacterial cells numbers after immobilization and bacterial cells after preservation as bacterial response to the immobilization and preservation. Heat activation was significantly affecting the chemical composition, carrier surface area, and pore volume. Highest surface area, pore volume, and Si/Al ratio were obtained in 110oC pretreated zeolite followed by 300oC pretreated zeolite. The bacterial cells obtained after immobilization process was 1,8x107 cfu/g, 3,0 x 107 cfu/g, and 2,1x107 for raw zeolite, 110oC pretreated zeolite and 300oC zeolite respectively. After 4 months preservation, the slight reduction of the bacterial cells was observed. Immobilization halophilic archaeae using zeolite as carrier was proven as low cost and effective preservation method due to relatively simple process and unspecific preservation temperature requirements.
Pengaruh Waktu Kontak terhadap Daya Adsorpsi Karbon Aktif pada Proses Purifikasi CH4 dari Biogas Nani Harihastuti; Silvy Djayanti; Rame
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 7 No. 2 (2016)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2016.v7.no2.p57-66

Abstract

Direct utilization of biogas as a fuel often cause problems, i.e bad odor, metal corrosive, wall erosion and frequent fire extinguished. This is due to the presence of gas impurities in biogas such as H2S, NH3, CO2, H2, CO and water vapor (H2O), which will lower the value of calories from biogas and detrimental to the environment and health. The purpose of this research is to eliminate impurities gases, through a process called gas purification, until higher heat value of biogas is achieved. The methods were undertaken by adsorption process using activated carbon adsorbent with variable time contacts, to obtain the optimum adsorption power of activated carbon toward gas impurities that are present in wastewater. The results showed that removal/reduction of H2S of 99.99% was obtained from 4200 ppm to 0.22 ppm, NH3 removal reached 12.7%, from 94.96 ppm to 0.65 ppm, CO2 removal reached 77.48%, from 30,77% to 6.93%, water vapour (H2O) removal reached 97.95%, from 0.584 mg/L to 0.012 mg/L, which increased methane concentration (CH4) from 38.2% to 84.12%. This methane purification technology is renewable energy sources that is safe and environmentally friendly and also can be developed in other industries i.e tofu industries. The saturation of activated carbon adsorbents obtained after 48 hours. Adsorption of each impurities gas components by activated carbon are written as follows: 10.98 mg H2S/gram activated carbon/minute, 0.016 mg NH3/gram of activated carbon/minute, 0.090 CO2/gram activated carbon/minute.
Efficient Cell-Wall Disruption of Microalgae Chlorella Vulgaris in water by catalytic ozonation over Microporous Carbon-Supported Titanium Oxide Rame Rame; Nilawati Nilawati; Silvy Djayanti; Novarina Irnaning Handayani; Agus Purwanto; Lisa Ruliaty; Ganang Dwi Harjanto
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 2 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no2.p30-36

Abstract

This study investigated several parameters for cell-wall disruption from microalgae Chlorella vulgaris during catalytic ozonation over microporous carbon-supported titanium oxide, including flow ozone, catalytic time, and reactor capacity. At the same time, the cell-wall disruption yield and an active compound yield such as chlorophyll and carotenoid were evaluated for each pretreatment. The required delivered flow ozone to achieve 76,47% cell-wall disruption of Chlorella vulgaris was 1 minute at 4 LPM, which produced chlorophyll 56,75% and carotenoid 89,09%. Carbon-supported titanium oxide reduces the required O3 dose and catalytic time for cell-wall disruption; however, it limited chlorophyll yield did not exceed 75,67%. Pretreatment with 1 minute at 1 LPM in 2 liters produced carotenoid yield by approximately 98,18%, though it reduced chlorophyll to 59,45%.
High Performance of Enzymatic Bioprocess for Production of Biomassed-based Bioethanol of Sago Palm Fiber Waste Nani Harihastuti; Rame Rame; Silvy Djayanti
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 2 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no2.p37-45

Abstract

Biomass waste in the form of fiber dregs contains many components of lignocellulose and hemicellulose. Lignocellulose can be used to produce ethanol through enzymatic biotechnology processes. Sago palm fiber industry is one potential industry producing biomass waste in the form of solid waste of fiber dregs (about 30% from the weight of processed raw materials). Solid fiber waste contains crude fiber and lignocellulose compound consists of cellulose (35-50%), hemicellulose ( 20-35%) and lignin (12-20%). This study aimed to utilize solid waste of sago palm fiber as a raw material of bioethanol production through enzymatic biotechnology processes of delignification, saccharification and fermentation which was then purified by distillation process to get ethanol. Delignification, saccharification, and fermentation stages are conducted using Phanerochaete chrysosporus Mushroom, Trichoderma viride fungus, and saccharomyces cerevisiae, respectively and then purified by distillation process (one level) to produce ethanol. The process of saccharification and fermentation process were done in an integrated manner (addition of Trichoderma viride and yeast saccharomyces cereviceae fungi done simultaneously). The weight of raw materials of solid waste fiber treated was about 6 kgs. Alcohol content obtained was about ± 4% (distillation). No water, air, and soil pollution inflicted, more added value, and sustainable were the main benefits of biotech process or bioprocess.
Kajian Penerapan Produksi Bersih di Industri Tahu di Desa Jimbaran, Bandungan, Jawa Tengah Silvy Djayanti
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 6 No. 2 (2015)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2015.v6.no2.p75-80

Abstract

Peran industri kecil di beberapa wilayah Kabupaten Semarang sangat penting dalam menunjang perekonomian, khususnya di sekitar lokasi industri tersebut. Salah satu industri kecil yang memiliki potensi berkembang adalah industri tahu. Di Jawa tengah ada sekitar 500 industri tahu, yang sampai saat ini masih terus mengembangkan kapasitas produksinya. Industri tahu adalah salah satu industri kecil yang berpotensi menyebabkan masalah lingkungan. Hampir seluruh industri kecil, terutama industri tahu, sebagian besar tidak memiliki instalasi pemanfaatan limbah dan pengolahan limbah, karena dibutuhkan investasi yang besar untuk membangun unit tersebut. Dengan melihat masalah tersebut, pendekatan yang dapat menyelesaikan masalah tersebut adalah dengan cara menerapkan produksi bersih. Kajian penerapan teknologi bersih ini dilakukan dengan metode quick scanning pada setiap tahapan proses produksi. Proses produksi tahu terdiri dari pemilihan kedelai, penimbangan kedelai, perendaman, pencucian, penggilingan, ekstraksi, penyaringan, pemasakan, penggumpalan, pemisahan whey, pembungkusan, pengepresan, pemasakan, dan pengemasan. Pada proses pembuatan tahu di industri ini memiliki perbedaan dari pembuatan tahu di Industri tahu lain, yaitu terdapat penambahan garam dan bawang putih untuk menambah sedap produk tahu. Hasil kajian penerapan produksi bersih di industri tahu adalah good house keeping, recycle, reduce dan reuse. Berdasarkan beberapa kriteria kelayakan, alternatif penerapan produksi bersih untuk industri ini berupa modifikasi tungku yang dilengkapi dengan cerobong asap, modifikasi alat penyaringan dengan mesin, dan pembangunan instalasi digester untuk penghasil biogas. Kesimpulan kajian ini industri tahu ini belum mengenal produksi bersih. Manfaat yang diperoleh dari penerapan produksi bersih berupa penghematan bahan bakar, peningkatan rendemen filtrat tahu, dan kebersihan lingkungan.
Co-Authors Agus Purwanto Ganang Dwi Harjanto Lisa Ruliaty Nani Harihastuti Nanik Indah Setianingsih Nasuka Nasuka Nilawati Nilawati Ningsih Ika Pratiwi Novarina Irnaning Handayani Rame Rame Rame Rizal Awaludin Malik