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All Journal Jurnal Riset Teknologi Pencegahan Pencemaran Industri
Rame Rame
Balai Besar Teknologi Pencegahan Pencemaran Industri
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Environmental Science

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Low Energy Bacteria Preservation of Extremely Halophilic Archaea Haloferax Lucentense and Haloferax Chudinovii Immobilized using Natural Zeolite Rizal Awaludin Malik; Nilawati Nilawati; Novarina Irnaning Handayani; Rame Rame; Silvy Djayanti; Ningsih Ika Pratiwi; Nanik Indah Setianingsih; Nasuka Nasuka
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 2 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no2.p16-28

Abstract

The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research was the type of the carrier which was raw zeolite, 110oC and 300oC heat-activated zeolite carrier, parameters measured in this study was physical and chemical of zeolite such as chemical content, Si/Al ratio, surface area and pore volume, and biochemical assay, bacterial cells numbers after immobilization and bacterial cells after preservation as bacterial response to the immobilization and preservation. Heat activation was significantly affecting the chemical composition, carrier surface area, and pore volume. Highest surface area, pore volume, and Si/Al ratio were obtained in 110oC pretreated zeolite followed by 300oC pretreated zeolite. The bacterial cells obtained after immobilization process was 1,8x107 cfu/g, 3,0 x 107 cfu/g, and 2,1x107 for raw zeolite, 110oC pretreated zeolite and 300oC zeolite respectively. After 4 months preservation, the slight reduction of the bacterial cells was observed. Immobilization halophilic archaeae using zeolite as carrier was proven as low cost and effective preservation method due to relatively simple process and unspecific preservation temperature requirements.
Efficient Cell-Wall Disruption of Microalgae Chlorella Vulgaris in water by catalytic ozonation over Microporous Carbon-Supported Titanium Oxide Rame Rame; Nilawati Nilawati; Silvy Djayanti; Novarina Irnaning Handayani; Agus Purwanto; Lisa Ruliaty; Ganang Dwi Harjanto
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 2 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no2.p30-36

Abstract

This study investigated several parameters for cell-wall disruption from microalgae Chlorella vulgaris during catalytic ozonation over microporous carbon-supported titanium oxide, including flow ozone, catalytic time, and reactor capacity. At the same time, the cell-wall disruption yield and an active compound yield such as chlorophyll and carotenoid were evaluated for each pretreatment. The required delivered flow ozone to achieve 76,47% cell-wall disruption of Chlorella vulgaris was 1 minute at 4 LPM, which produced chlorophyll 56,75% and carotenoid 89,09%. Carbon-supported titanium oxide reduces the required O3 dose and catalytic time for cell-wall disruption; however, it limited chlorophyll yield did not exceed 75,67%. Pretreatment with 1 minute at 1 LPM in 2 liters produced carotenoid yield by approximately 98,18%, though it reduced chlorophyll to 59,45%.
High Performance of Enzymatic Bioprocess for Production of Biomassed-based Bioethanol of Sago Palm Fiber Waste Nani Harihastuti; Rame Rame; Silvy Djayanti
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 2 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no2.p37-45

Abstract

Biomass waste in the form of fiber dregs contains many components of lignocellulose and hemicellulose. Lignocellulose can be used to produce ethanol through enzymatic biotechnology processes. Sago palm fiber industry is one potential industry producing biomass waste in the form of solid waste of fiber dregs (about 30% from the weight of processed raw materials). Solid fiber waste contains crude fiber and lignocellulose compound consists of cellulose (35-50%), hemicellulose ( 20-35%) and lignin (12-20%). This study aimed to utilize solid waste of sago palm fiber as a raw material of bioethanol production through enzymatic biotechnology processes of delignification, saccharification and fermentation which was then purified by distillation process to get ethanol. Delignification, saccharification, and fermentation stages are conducted using Phanerochaete chrysosporus Mushroom, Trichoderma viride fungus, and saccharomyces cerevisiae, respectively and then purified by distillation process (one level) to produce ethanol. The process of saccharification and fermentation process were done in an integrated manner (addition of Trichoderma viride and yeast saccharomyces cereviceae fungi done simultaneously). The weight of raw materials of solid waste fiber treated was about 6 kgs. Alcohol content obtained was about ± 4% (distillation). No water, air, and soil pollution inflicted, more added value, and sustainable were the main benefits of biotech process or bioprocess.
PENGGUNAAN KHAMIR RHODOTORULA MUCILAGINOSA UNTUK BIOSENSOR BOD MENGGUNAKAN ELEKTRODA EMAS Rame Rame
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 5 No. 2 (2014)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2014.v5.no2.p37-44

Abstract

Aplikasi biosensor BOD pada dasarnya meningkat seiring dengan berkembangnya keperluan manusia dan kemajuan IPTEK. Tetapi secara umum tetap didominasi untuk aplikasi dibidang medis dan lingkungan hidup.Biosensor BOD berdasarkan metabolisme khamir dikembangkan dalam rangka mempersingkat waktu pengukuran nilai BOD untuk monitoring lingkungan. Pengembangan biosensor dibuat melalui imobilisasi khamir pada film tipis dalam matrik agarose dengan Nafion sebagai membran untuk proses pertukaran ion. Khamir diambil dari fermentasi Rhodotorula mucilaginosa. Film tipis kemudian dilekatkan pada emas sebagai elektroda kerja. Biosensor BOD dikalibrasi menggunakan larutan yang mengandung glukosa yang setara sebagai sampel standar. Kondisi optimum diamati dengan waktu tunggu pengukuran 20 menit pada potensial 500 mV (vs Ag/AgCl). Untuk analisis glukosa, waktu tunggu adalah 20 menit dengan batas deteksi 1 mg/l. Hubungan linier yang baik diperoleh dari larutan standar glukosa yaitu, 2 0.99 dan estimasi kesalahan pengukuran 2,45%. Nilai BOD yang diperoleh dari biosensor menunjukkan perbandingan yang baik dengan konvensional.
Co-Authors Agung Budiarto Agus Purwanto Agus Purwanto Aris Mukimin Cholid Syahroni Ganang Dwi Harjanto Lisa Ruliaty Misbachul Moenir Nani Harihastuti Nanik Indah Setianingsih Nasuka Nasuka Nilawati Nilawati Ningsih Ika Pratiwi Novarina Irnaning Handayani Rizal Awaludin Malik Silvy Djayanti