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All Journal Jurnal Riset Teknologi Pencegahan Pencemaran Industri
Novarina Irnaning Handayani
Balai Besar Teknologi Pencegahan Pencemaran Industri
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Environmental Science

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Low Energy Bacteria Preservation of Extremely Halophilic Archaea Haloferax Lucentense and Haloferax Chudinovii Immobilized using Natural Zeolite Rizal Awaludin Malik; Nilawati Nilawati; Novarina Irnaning Handayani; Rame Rame; Silvy Djayanti; Ningsih Ika Pratiwi; Nanik Indah Setianingsih; Nasuka Nasuka
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 2 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no2.p16-28

Abstract

The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research was the type of the carrier which was raw zeolite, 110oC and 300oC heat-activated zeolite carrier, parameters measured in this study was physical and chemical of zeolite such as chemical content, Si/Al ratio, surface area and pore volume, and biochemical assay, bacterial cells numbers after immobilization and bacterial cells after preservation as bacterial response to the immobilization and preservation. Heat activation was significantly affecting the chemical composition, carrier surface area, and pore volume. Highest surface area, pore volume, and Si/Al ratio were obtained in 110oC pretreated zeolite followed by 300oC pretreated zeolite. The bacterial cells obtained after immobilization process was 1,8x107 cfu/g, 3,0 x 107 cfu/g, and 2,1x107 for raw zeolite, 110oC pretreated zeolite and 300oC zeolite respectively. After 4 months preservation, the slight reduction of the bacterial cells was observed. Immobilization halophilic archaeae using zeolite as carrier was proven as low cost and effective preservation method due to relatively simple process and unspecific preservation temperature requirements.
Efficient Cell-Wall Disruption of Microalgae Chlorella Vulgaris in water by catalytic ozonation over Microporous Carbon-Supported Titanium Oxide Rame Rame; Nilawati Nilawati; Silvy Djayanti; Novarina Irnaning Handayani; Agus Purwanto; Lisa Ruliaty; Ganang Dwi Harjanto
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 2 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no2.p30-36

Abstract

This study investigated several parameters for cell-wall disruption from microalgae Chlorella vulgaris during catalytic ozonation over microporous carbon-supported titanium oxide, including flow ozone, catalytic time, and reactor capacity. At the same time, the cell-wall disruption yield and an active compound yield such as chlorophyll and carotenoid were evaluated for each pretreatment. The required delivered flow ozone to achieve 76,47% cell-wall disruption of Chlorella vulgaris was 1 minute at 4 LPM, which produced chlorophyll 56,75% and carotenoid 89,09%. Carbon-supported titanium oxide reduces the required O3 dose and catalytic time for cell-wall disruption; however, it limited chlorophyll yield did not exceed 75,67%. Pretreatment with 1 minute at 1 LPM in 2 liters produced carotenoid yield by approximately 98,18%, though it reduced chlorophyll to 59,45%.
Komparasi Analisis Total Coliform dan Coli Tinja dengan Menggunakan Metode Most Propbable Number (MPN) 5 Tabung dan Enzim Substrat Novarina Irnaning Handayani
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 7 No. 2 (2016)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2016.v7.no2.p105-112

Abstract

Dalam pemantauan kualitas lingkungan untuk air minum, air bersih, air limbah, dan air sungai, mensyaratkan aspek mikrobiologi parameter coliform dan atau coli tinja sebagai indikator pencemaran lingkungan. Pada saat ini metode yang paling banyak digunakan adalah Number (MPN) 5 tabung yang memiliki waktu pengerjaan minimal 4 hari. Penelitian ini dilakukan untuk menjajaki kemungkinan pemakaian metode lain yang telah terstandarisasi dan memiliki beberapa keunggulan. Metode yang dipilih adalah enzim substrat. Uji laboratorium dilakukan dengan membandingkan hasil analisis antara MPN 5 tabung dan enzim substrat, masing-masing dengan ulangan 7 kali. Hasil komparasi menunjukkan bahwa analisa dengan menggunakan metode enzim substrat memberikan hasil yang lebih besar atau lebih sensitif dibanding MPN 5 tabung. Pada sampel yang mengandung bakteri coli yang tinggi, dalam hal ini pada sampel air limbah dan air sungai perbedaan hasil antara MPN 5 tabung dan enzim substrat sangat signifikan, sedangkan pada sampel dengan kandungan coli kecil atau tidak ada, hasil keduanya sama. Metode enzim substrat sangat direkomendasikan untuk sampel air minum dan air bersih sehingga akan memberikan hasil yang lebih meyakinkan bagi penggunanya. Kandungan coliform pada sampel air minum dan air bersih walaupun sangat kecil akan benar-benar terdeteksi oleh metode yang memiliki sensitifitas lebih baik.
PENGOLAHAN AIR LIMBAH INDUSTRI KARTON BOX DENGAN METODE INTEGRASI UPFLOW ANAEROBIC SLUDGE BED REACTOR (UASB) DAN ELEKTROKOAGULASI-FLOTASI Hanny Vistanty; Aris Mukimin; Novarina Irnaning Handayani
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 6 No. 1 (2015)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2015.v6.no1.p1-8

Abstract

Penelitian ini bertujuan untuk menginvestigasi kinerja teknologi integrasi Upflow Anaerobic Sludge Bed Reactor (UASB) dengan elektrokoagulasi-flotasi (ECF) sebagai unit pengolah air limbah industri karton box dalam berbagai kondisi operasi. Sebelum diaplikasikan, unit UASB diawali dengan proses aklimatisasi selama 7 hari menggunakan dua jenis substrat, yaitu gula dan pati. Operasional UASB secara kontinyu dilakukan pada berbagai OLR dan HRT konstan (24 jam). Air limbah terproses UASB kemudian dielektroflotasi menggunakan anoda alumunium (Al) dan besi (Fe). Optimalisasi proses ECF dikaji pada berbagai variabel pH dan waktu elektrolisis. Proses UASB dengan susbtrat pati menunjukkan efektivitas aklimatasi yang tinggi dibanding subtrat gula. Kondisi steady-state akan tercapai setelah 6 hari operasional dengan efisiensi penurunan COD 91% dan OLR 25 kg COD/m3 hari. Aplikasi UASB secara kontinyu telah mampu menurunkan COD 94% dengan waktu tinggal 24 jam. Proses ECF mampu menurunkan COD air limbah terolah UASB sekitar 70-81%. Kondisi optimum penurunan COD tercapai pada pH 7,5 untuk anoda Al dan pH 6 atau 9 untuk anoda Fe. Penambahan waktu elektrolisis di atas 10 menit sangat mempengaruhi efektivitas penurunan COD untuk anoda Fe sedangkan anoda Al tidak terjadi penurunan yang signifikan. Jumlah sludge yang dihasilkan oleh proses ECFsebanyak 4 kg/m3 untuk anoda Al dan 5 kg/m3 untuk anoda Fe. Biaya kebutuhan energi berkisar antara 4,5 hingga 18 kWh/m3dan konsumsi elektroda sebanyak 0,17 kg Al/m3 atau 0,515 kg Fe/m3. Integrasi UASB dan ECF berpotensi untuk diaplikasikan sebagai sistem pengolahan air limbah industri karton box yang efektif.
PEMANFAATAN KONSORSIUM MIKROBIA UNTUK MENINGKATKAN KINERJA SISTEM LUMPUR AKTIF Novarina Irnaning Handayani
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 6 No. 1 (2015)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2015.v6.no1.p17-22

Abstract

Industri tekstil sebagian besar menggunakan mengolah limbah cair pada instalasi pengolahan air limbah dengan menggunakan sistem fisika, kimia, dan biologi. Sistem biologi yang digunakan biasanya adalah lumpur aktif yang terkadang mengalami gangguan. Tujuan penelitian ini adalah membuat konsorsium mikrobia terpilih yang dapat menaikkan kinerja lumpur aktif yang sedang terganggu, diindikasikan dengan turunnya nilai sludge volume dalam reaktor lumpur aktif serta menurunkan COD air limbah terolah. Terpilih 6 (enam) jenis bakteri non patogen yaitu Bacillus macerans, Bacillus subtilis, Bacillus thuringiensis, Bacillus sp, Kurthia zopfii,dan Pseudomonas stutzeri untuk digabungkan dalam satu konsorsium. Hasil uji antagonisme antar species terpilih menunjukkan tidak munculnya zone penghambatan, sehingga 6 (enam) jenis bakteri tersebut dapat digabungkan menjadi satu kesatuan.Hasil uji coba laboratorium menunjukkan konsorsium yang ditambahkan nutrien berupa 25 gr bekatul dan 50 gram gula per liter air dengan pencapaian sludge volume 30 menit 85% dan setelah diendapkan 24 jam adalah 35% denganpenurunan COD 82% Uji coba lanjutan menunjukkan bahwa konsorsium dalam 6 jenis bakteri ditambah Nitrobacter dan yeast sludge volume 30 menit terbaik mencapai 73% setelah diendapkan 24 jam menjadi 32% dengan penurunan COD mencapai 81%.
Performance of Immobilized-Selected Microorganisms in The Biodegradation of Textile Industry Waste Water Novarina Irnaning Handayani; Nanik Indah Setianingsih; Misbachul Moenir
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 1 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no1.p29-37

Abstract

Waste water from textile industry contain pollutant whit in certain concentration. To protect the environment and water bodies, wastewater containing pollutant must be treated before discharging into the environment. Anaerobic biological treatment has been used as a method in treating textile industry waste water. Several factors of conventional anaerobic treatment needs to be repaired in order to improve the performance of treating wastewater. A selected microorganisms as inoculum was expected to increase effectivity of waste water biodegradation. In this study a selected microorganisms was used as inoculum in the form of immobilized and free cells in anaerobic treatment of waste water from textile industry and compared with conventional sludge. Results from this study show that selected-immobilized microorganisms achieved the best performance due to its stability and efficiency in removing pollutant in the waste water. Mean while microorganisms in the form of free cells got the lowest performance in treating waste water, estimated due to its sensitivity of environmental conditions and having low mechanical strength of biomass. Immobilized cells succesfully treated waste water from textile industry, removal of pollutant in suspended solid parameter reached on 93,78% mean while, in oil & grease, BOD5 and COD parameter reached on 99,13%, 81,54% and 64,94% respectively. Pollutant in amonia parameter has not been fully treated in this experiment due to condition of the experiment system was anaerobic instead of aerobic.
Full Scale Application of Integrated Upflow Anaerobic Filter (UAF)-Constructed Wetland (CWs) in Small Scale Batik Industry Wastewater Treatment Novarina Irnaning Handayani; Rustiana Yuliasni; Nanik Indah Setianingsih; Agung Budiarto
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 11 No. 1 (2020)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2020.v11.no1.p27-35

Abstract

This research aimed to evaluate the implementation of integrated Upflow Anaerobic Filter (UAF)-Constructed Wetlands (CWs) in real condition of wastewater treatment plant in batik small scale industry. The full scale reactor consisted of equalization chamber with HRT of 2 days; Upflow Anaerobic Filter (UAF) with HRT of 6 days, and Horizontal Subsurface Constructed Wetlands (HSSCWs) with HRT of 1.5 days. The UAF- CWs integrated technology was used to treat batik wastewater with COD inlet of 1339 – 2034 mg/L and pH of 9.0 – 9.4. This study showed that single UAF technology alone was able to reduced 56 – 78%, while the integration UAF –wetland technology improved the performance to 85% and reduced the pH into 7.5 – 7.8
Co-Authors Agung Budiarto Agus Purwanto Agus Purwanto Aris Mukimin Ganang Dwi Harjanto Hanny Vistanty Hanny Vistanty Lisa Ruliaty Lisa Ruliaty Misbachul Moenir Nanik Indah Setianingsih Nasuka Nasuka Nilawati Nilawati Ningsih Ika Pratiwi Rame Rame Rame Rame Rizal Awaludin Malik Rustiana Yuliasni Silvy Djayanti Silvy Silvy Djayanti