Rizal Awaludin Malik
Balai Besar Teknologi Pencegahan Pencemaran Industri

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Low Energy Bacteria Preservation of Extremely Halophilic Archaea Haloferax Lucentense and Haloferax Chudinovii Immobilized using Natural Zeolite Rizal Awaludin Malik; Nilawati Nilawati; Novarina Irnaning Handayani; Rame Rame; Silvy Djayanti; Ningsih Ika Pratiwi; Nanik Indah Setianingsih; Nasuka Nasuka
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 2 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no2.p16-28

Abstract

The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research was the type of the carrier which was raw zeolite, 110oC and 300oC heat-activated zeolite carrier, parameters measured in this study was physical and chemical of zeolite such as chemical content, Si/Al ratio, surface area and pore volume, and biochemical assay, bacterial cells numbers after immobilization and bacterial cells after preservation as bacterial response to the immobilization and preservation. Heat activation was significantly affecting the chemical composition, carrier surface area, and pore volume. Highest surface area, pore volume, and Si/Al ratio were obtained in 110oC pretreated zeolite followed by 300oC pretreated zeolite. The bacterial cells obtained after immobilization process was 1,8x107 cfu/g, 3,0 x 107 cfu/g, and 2,1x107 for raw zeolite, 110oC pretreated zeolite and 300oC zeolite respectively. After 4 months preservation, the slight reduction of the bacterial cells was observed. Immobilization halophilic archaeae using zeolite as carrier was proven as low cost and effective preservation method due to relatively simple process and unspecific preservation temperature requirements.
Pengolahan Air Limbah Industri Bakery Menggunakan Sistem Stripper-Lumpur Aktif Rizal Awaludin Malik; Hanny Vistanty; Sartamtomo; Nanik Indah Setianingsih; Farida Crisnaningtyas; Nur Zen
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 7 No. 2 (2016)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2016.v7.no2.p89-98

Abstract

Air limbah industri bakery memiliki karakteristik kandungan organik, lemak, dan surfaktan yang tinggi. Keberadaan surfaktan pada air limbah dapat mengganggu proses degradasi kandungan organik dan lemak pada instalasi pengolahan air limbah khususnya pengolahan secara biologis/lumpur aktif. Studi ini membahas tentang performa integrasi system stripper-lumpur aktif yang diaplikasikan untuk mengolah air limbah industry bakery dalam skala full scale. Proses stripper dilakukan dengan menggunakan kolom yang diintegrasikan dengan bak aerasi pada kisaran HRT (waktu tinggal) 24 jam. Proses stripper memberikan efisiensi enurunan COD dan surfaktan tertinggi sebesar 96% dan 80%. Proses lumpur aktif digunakan untuk mengolah keluaran dari proses stripper dengan karakteristik lumpur aktif MLSS 4000-4500 mg/L, FM/ rasio 0,268, sludge age 11 hari dan HRT 24 jam, proses degradasi dengan lumpur aktif ini mampu memberikan efisiensi penurunan COD dan surfaktan sebesar 79% dan 88%. Hasil penelitian menunjukkan bahwa unit IPAL dengan menggunakan sistem stripper-lumpur aktif memiliki efisiensi penurunan COD, TSS, dan BOD sebesar 98,9%, 87,9%, dan 96,4%, sehingga sistem ini merupakan teknologi yang tepat untuk diterapkan pada pengolahan limbah industri bakery.
Enhanced Performance Of Multi-Stage Anaerobic Digestion Of Tofu Wastewater: Role Of Recirculation Hanny Vistanti; Rizal Awaludin Malik
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 1 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no1.p29-37

Abstract

Continuous three-stage anaerobic reactors with recirculation system were employed to treat real tofu wastewater. The system consists of 3 reactors connected in series and effluent of the last reactor was partly returned as feed of the first reactor along with fresh wastewater. The recirculation system was conducted on four variations of recirculation ratio (RR)s of 0, 1.0, 1.3, and 1.5 to evaluate the influence of recirculation on stability and performance of anaerobic system. Results showed that recirculation improved the performance of anaerobic digestion. COD was fluctuated without recirculation and gradually decreased to 373 and 298 mg/L at RR 1.3 and 1.5, respectively. Likewise, pH value was dropped to 6.5 at RR 0, and started to increase to approximately 8.0 upon employing recirculation. In addition, the system also showed excellent toleration against rise in ammonia loading, with effluent ammonia approximately 4.678 mg/L. The findings showed that the system employed had excellent efficiency and stability in treating tofu wastewater.
Kemampuan Isolat Bakteri Haloferax Spp dalam Meningkatkan Kemurnian Garam NaCl pada Proses Kristalisasi Nilawati; Marihati; Rizal Awaludin Malik
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 8 No. 2 (2017)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2017.v8.no2.p92-103

Abstract

Garam yang dihasilkan oleh peladangan garam rakyat tidak memenuhi standar kemurnian (84-90%), maka diperlukan pencucian garam untuk meningkatkan kemurnian NaCl yang akan digunakan sebagai bahan baku industri. Pencucian garam akan menghasilkan air limbah yang dibuang ke lingkungan sebanyak 1,5 m3 untuk 10 ton garam yang dicuci. Peladangan garam dengan menggunakan bantuan mikroorganisme yang hidup pada salinitas tinggi (halofilik) merupakan suatu teknologi alternatif dalam produksi garam NaCl yang memiliki kemurnian yang tinggi dan ramah lingkungan. Penelitian ini bertujuan untuk mengetahui kualitas garam NaCl yang dihasilkan berdasarkan kemampuan bakteri Halococcus spp dalam mempengaruhi faktor-faktor fisika-kimiapada saat proses kristalisasi garam. Penelitian ini terbagi atas dua tahap yaitu penentuan jumlah starter untuk kristalisasi dan percobaan kristalisasi. Parameter yang di ukur adalah absorbansi (OD 600), kemurnian NaCl hasil kristalisasi, kekentalan larutan garam (oBe), kekeruhan (transmittance), warna kristal, dan pengamatan mikroskopis. Variabel yang digunakan pada penelitian pertama adalah komposisi inokulum yang akan digunakan sebagai starter, dan variabel pada penelitian kedua merupakan variabel pembanding yaitu air garam tua dengan penambahan konsorsium halofilik, penambahan nutrisi LB (Luria berthani) dan kontrol berupa air garam tua. Hasil penelitian menunjukan bahwa komposisi terbaik untuk proses kristalisasi adalah konsentrasi isolat 10% dan penambahan pada proses kristalisasi sebanyak 1%. Pada penelitian kedua kenaikan kekentalan larutan garam tercepat diperoleh pada perlakuan Halococcus spp dan kontrol+LB, kekeruhan tertinggi didapatkan oleh perlakuan konsorsium halofilik, dan kekompakan kristal garam yang terbaik dihasilkan oleh perlakuan Halococcus spp. Kemurnian NaCl yang dihasilkan dari penambahan bakteri Halococcus spp memiliki nilai tertinggi dibandingkan perlakuan lainnya yaitu 94,64%, sementara perlakuan dengan menggunakan konsorsium bakteri halofilik sebesar 92,84%, kontrol dengan penambahan nutrisi LB 92,51% dan kontrol 91%.
Performance of a Full-Scale Anaerobic Digestion on Bakery Wastewater Treatment : Effect of Modified Distribution System Hanny Vistanti; Rizal Awaludin Malik; Aris Mukimin
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 11 No. 1 (2020)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2020.v11.no1.p12-18

Abstract

The effectiveness of a full-scale anaerobic digestion pretreatment was evaluated and the effect of wastewater distribution system was determined on the performance of bakery wastewater (BW) treatment. The BW was fed to 3 series of anaerobic compartments as the main degradation process. The distribution system of first compartment was modified and circulated to enhance contact and efficiency. While the effluent of last compartment was partly returned to the first compartment as an external circulation and the other part was further processed in activated sludge under aerobic conditions. The overall system was able to remove chemical oxygen demand (COD), total suspended solids (TSS), and biochemical oxygen demand (BOD) up to 97.7%, 99.7%, and 99.6%, respectively, at maximum organic loading rate of 6.3 kg COD/m3day. High removal of pollutants indicated that modified distribution of circulation is advantageous to the BW treatment