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All Journal BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Biosaintifika: Journal of Biology & Biology Education BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Jurnal Biogenerasi Jurnal Sains Agro Jurnal Agroteknologi Merdeka Pasuruan Journal of Innovative and Creativity
Idramsa Idramsa, Idramsa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Social Sciences Other

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Molecular Identification of Endophytic Fungi from Bark of Raru (Cotylelobium melanoxylon) that Produce the Antibacterial Compounds Hasanah, Uswatun; Riwayati, Riwayati; Idramsa, Idramsa; Prasetya, Eko
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9424

Abstract

The Dipterocarpaceae plant, raru (Cotylelobium melanoxylon) is widespread in Southeast Asia. The bark of raru has been used by local communities in North Sumatera as antidiarrheal drugs due to its antibacterial compounds. The antibacterial activity of the rarus bark is induced by endophytic fungi that live in the region of the bark. This study aimed to identify the endophytic fungi-producer of antibacterial compounds in the bark of raru (C. melanoxylon) by means of molecular analysis. In general, endophytic fungi have the ability to inhibit the growth of pathogenic bacteria. Thirty-eight isolates of endophytic fungi were isolated from the bark of raru. Selection of isolates for antibacterial activity against Escherichia coli ATCC 35218 and Staphylococcus aureus ATCC 25923 used the dual culture assay. Selection using the dual culture assay yielded 6 endophytic fungal isolates that have the ability to inhibit the growth of test bacteria. EF10A sample was the most powerful isolate inhibiting the growth of both bacteria test. Those six bacteria molecularly identified used a sequence generated from ITS rDNA region. Based on rDNA ITS region sequences, isolate, the producers of the antibacterial compound were identified as Talaromyces cellulolyticus, Penicillium purpurogenum, Aspergillus sp., Trichoderma harzianum, and Aspergillus orizae. The results of this study can be used by researchers to explore more potential endophytic fungi in raru plants (C. melanoxylon) as a source of medicine. The data obtained need to be supported by further research to isolate the bioactive compounds that can inhibit the growth of microbial pathogens.
Development of Worksheet Based on Search Solve Create and Share (SSCS) on Learning Interest, Science Process Skills and Student Learning Outcomes in Biology Material of First Level (X Class) Senior High School Gultom, Josephine Olivia; Idramsa, Idramsa; Harahap, Fauziyah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.5833

Abstract

This study develops and tests (LKPD) based on search, solve, create, and share (SSCS) in biology learning for class X MIA Semester I at SMA Negeri 5 Medan. To actively assess students' learning interests, science process skills, and learning outcomes at school because this process helps students search, find, and build their knowledge to solve problems. This research is development. Uses 4D development research (Define, Design, Develop, Disseminate). Interviews, material expert validation questionnaires, design validation questionnaires, language validation questionnaires, study field teacher responses to SSCS-based LKPD, student learning interest questionnaires, student science process skills questionnaires, and pretest-posttest assessment sheets were used. Material experts rate material validity as "Very Good/Decent" with an average of 91.75%. Design expert product quality results averaged 87.5% "Good/Decent". Teacher responses on the SSCS-based LKPD averaged 86.25% "Good/Decent". Students are 71% "Interested" in learning in small groups and 88% "Very Interested" in large groups. Science students' process skills averaged 86.67 in the big group and 76.67 in the small group in the "Good/Decent" category. Out of 37 students, 4 had a "Medium" N-gain of 0.7 on the biodiversity and virus meters, whereas 33 had a "High" N-gain of 0.8.
Pengembangan Buku Nonteks Berbasis Riset Karakterisasi Salak Padangsidimpuan (Salacca Sumatrana Becc.) Rahma, Aisyah; Hasairin, Ashar; Idramsa, Idramsa
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13462

Abstract

This study aims to produce a non-textbook product about the development of a non-textbook based on research into the characterization of Salak Padangsidimpuan (Salacca Sumatrana Becc.). This study uses research and development (R&D) methods with the 4D Thiagarajan development model, which includes the stages of definition, design, development, and dissemination. The results of the study indicate that product validation by content experts obtained a score of 89.1%, categorized as "very feasible." Language experts gave a score of 81%, also categorized as "very feasible," while learning design experts assessed the product with a score of 92.3%, falling into the same category. Feedback from biology lecturers showed that the book is suitable for use, with an average score of 88.9%, classified as "very feasible." In user trials, individual trials resulted in a score of 81.9%, small group trials 82.6%, and limited group trials reached 82.2%, all in the "very feasible category”. In conclusion, the developed non-textbook is highly suitable for use as a reference in teaching spermatophyte taxonomy for biology students. Keywords: Non-textbook, Padangsidimpuan, Development, Salacca Sumatrana
Efektivitas Ecoenzyme Limbah Kulit Jeruk Sebagai Pupuk Organik Cair Terhadap Pertumbuhan Vegetatif Bayam Hijau (Amaranthus hybridus) Siahaan, Eliza Sriyuliani; Siregar, Aidilia Syafitri; Anggraini, Jihan Indah; Br Tambunan, Mei Cristina; Triana, Rennyta; Atmaja, Wia Purwa; Syahputri, Yulia Nanda; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Sains Agro Vol 10, No 1 (2025): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v10i1.1743

Abstract

Ecoenzyme merupakan hasil fermentasi limbah organik, gula, dan air yang mengandung enzim aktif, asam organik, serta unsur hara penting seperti nitrogen, fosfor, dan kalium. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ecoenzyme limbah kulit jeruk sebagai pupuk organik cair (POC) terhadap pertumbuhan bayam hijau (Amaranthus hybridus), serta menentukan konsentrasi yang paling optimal. Penelitian dilaksanakan di rumah kaca menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL), terdiri dari empat perlakuan yaitu kontrol (P0), P1 (1 ml ecoenzym + 200 ml air), P2 (1 ml + 400 ml air), dan P3 (1 ml + 600 ml air), masing-masing dengan enam ulangan. Parameter yang diamati meliputi tinggi tanaman, lebar daun, dan jumlah daun. Data dianalisis menggunakan ANOVA dilanjutkan dengan uji LSD taraf 5%. Hasil penelitian menunjukkan bahwa pemberian ecoenzyme berpengaruh signifikan terhadap tinggi tanaman, lebar daun dan jumlah daun. Perlakuan P1 (1 ml + 200 ml air) dan perlakuan P2 (1 ml ecoenzyme dalam 400 ml air) merupakan konsentrasi yang paling optimal dalam meningkatkan pertumbuhan bayam hijau dengan P1 paling efektif untuk tinggi dan lebar daun, serta P1 dan P2 untuk jumlah daun. Penelitian ini mendukung pemanfaatan ecoenzyme limbah kulit jeruk sebagai alternatif POC yang ramah lingkungan dan efisien dalam budidaya tanaman hortikultura
Efektivitas POC Berbasis Limbah Sayuran dan EM4 Terhadap Pertumbuhan Sawi (Brassica Juncea L.) Rindiani, Artika; Ramadhani, Gita Syahri; Sidabutar, Jeki; Liliana, Najwa; Sari Pane, Nita Maya; Nasution, Rifka Khairunnisa; Batubara, Zihan Zahriani; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Sains Agro Vol 10, No 1 (2025): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v10i1.1749

Abstract

Permasalahan limbah organik rumah tangga dan pasar tradisional yang tidak terkelola dengan baik dapat menimbulkan dampak negatif terhadap lingkungan. Salah satu solusi pemanfaatan limbah tersebut adalah melalui produksi pupuk organik cair (POC) yang ramah lingkungan. Penelitian ini bertujuan untuk mengevaluasi efektivitas pupuk organik cair yang dibuat dari kombinasi limbah sayuran, kulit nanas, dan air cucian beras dengan penambahan Effective Microorganisme 4 (EM4) terhadap pertumbuhan tanaman sawi manis (Brassica juncea L.). Penelitian dilaksanakan secara eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat perlakuan dosis POC: 0 ml (kontrol), 5 ml, 10 ml, dan 15 ml per 250 ml air, masing-masing dengan empat ulangan. Parameter yang diamati meliputi tinggi tanaman, jumlah daun, lebar daun, dan berat basah tanaman. Hasil penelitian menunjukkan bahwa pemberian POC secara signifikan meningkatkan pertumbuhan sawi manis pada semua parameter dibandingkan kontrol, dengan dosis optimal sebesar 15 ml POC+250 ml air. Kandungan hara makro POC, khususnya kalium (0,96%), serta kontribusi bioaktif dari bahan organik dan EM4, berperan dalam merangsang pertumbuhan vegetatif tanaman. Temuan ini menunjukkan bahwa pemanfaatan limbah organik menjadi POC tidak hanya mendukung pertanian berkelanjutan, tetapi juga menawarkan alternatif efektif dalam pengelolaan sampah organik
Pengaruh Pemberian Pupuk Organik Cair (POC) Terhadap Pertumbuhan Tanaman Bayam Merah (Amaranthus tricolor L.) Adelia, Ichlasul; Apriliani, Dwi; Sohada, Ummi; Febriani, Jesika; Batubara, Wardah Arijah; tampubolon, azarya debora; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Agroteknologi Merdeka Pasuruan Vol 9, No 1 (2025): JUNI 2025
Publisher : Universitas Merdeka Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51213/jamp.v9i1.127

Abstract

Penggunaan pupuk kimia yang berlebihan dalam pertanian modern menimbulkan dampak negatif terhadap kesuburan tanah dan lingkungan, sehingga diperlukan alternatif ramah lingkungan seperti pupuk organik cair (POC). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian POC dari nasi basi terhadap pertumbuhan tanaman bayam merah (Amaranthus tricolor L.) serta menentukan konsentrasi POC yang paling optimal. Metode penelitian yang digunakan adalah eksperimen dengan pendekatan deskriptif kuantitatif, yang dilaksanakan selama 12 hari di Rumah Kaca FMIPA Universitas Negeri Medan. Empat perlakuan konsentrasi POC diterapkan (0%, 15%, 25%, dan 35%) dengan parameter yang diamati meliputi tinggi tanaman, jumlah daun, dan panjang akar. Keunikan penelitian ini terletak pada pemanfaatan nasi basi sebagai bahan dasar POC yang masih jarang dikaji pada budidaya bayam merah. Hasil penelitian menunjukkan bahwa konsentrasi 35% memberikan hasil paling optimal dengan rata-rata tinggi tanaman 10,2 cm dan panjang akar 2,3 cm, dibandingkan kontrol yang hanya mencapai 5,8 cm dan 1,1 cm. Namun, jumlah daun tidak menunjukkan perbedaan signifikan di antara perlakuan. Hasil ini menunjukkan bahwa senyawa fermentasi nasi basi seperti asam amino dan enzim mendukung peningkatan pertumbuhan tanaman. Disimpulkan bahwa POC dari nasi basi berpotensi sebagai pupuk alternatif yang ramah lingkungan dan efektif. Disarankan penelitian lanjutan dilakukan dengan uji mikrobiologis untuk mengidentifikasi mikroba dominan dalam POC.
Efektivitas Pemberian Pupuk Organik Cair (POC) dari Limbah Kulit Pisang Kepok dan Kulit Mangga Terhadap Pertumbuhan Kangkung Darat (Ipomoea reptans) Utami, Mega; Siska Dewi, Alfina; Hikmal Romadhon, Galih; Maharani, Maura; Andini, Putri; Naufal, Waliid; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6291

Abstract

The excessive application of inorganic fertilizers in agriculture leads to environmental degradation, necessitating sustainable alternatives. This study evaluated the effectiveness of liquid organic fertilizer (LOF) formulated from banana peel, mango peel, and rice washing water on the vegetative growth of Ipomoea reptans. The research employed a Completely Randomized Design (CRD) with four treatment concentrations: 0 ml/L (control), 10 ml/L, 20 ml/L, and 30 ml/L, each replicated three times. Growth parameters assessed at 21 days after planting included plant height, number of leaves, number of roots, and fresh weight. Laboratory analysis revealed nutrient content of 0.04% nitrogen, 0.17% phosphorus, and 3.04% potassium, indicating a potassium-dominant formulation. The 10 ml/L treatment resulted in the highest plant height (18.00 cm), while the 30 ml/L treatment yielded the highest leaf number (9.33) and fresh weight (0.47 g). However, one-way ANOVA showed no statistically significant differences (p > 0.05) among treatments. The novelty of this research lies in utilizing a combination of commonly discarded household organic waste as a low-cost biofertilizer with measurable nutrient content. Although short-term results were statistically insignificant, the formulation demonstrates potential for refinement and longer application in sustainable agriculture
Identification of Microbial Contamination in Street Food Around Universitas Negeri Medan Utami, Larasati Arum; Idramsa, Idramsa; Rindiani, Artika; Liliana, Najwa; Pane, Nita Maya Sari; Nadeak, Santaro Marito
Journal of Innovative and Creativity Vol. 5 No. 2 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i2.2647

Abstract

Street food remains a popular choice for students due to its affordability and convenience. Its rapid preparation and service contribute to its ongoing popularity. The university, including Universitas Negeri Medan, is often surrounded by numerous street vendors offering a diverse range of foods, predominantly made from meat and flour. However, the open exposure of these foods and insufficient sanitation practices pose a heightened risk of contamination by harmful microorganisms. This study examines bacterial contamination in processed meat-based street foods sold near Universitas Negeri Medan, with a focus on items such as meatballs, grilled meatballs, and batagor. Microbiological examination involved isolating bacteria using selective and differential culture media, particularly Eosin Methylene Blue Agar (EMBA). Subsequent identification was carried out using Gram staining to determine cell morphology and SIM (Sulfide Indole Motility) medium for motility assessment in the microbiology lab at Universitas Negeri Medan. Findings revealed contamination predominantly in grilled meatballs, with bacteria belonging to the Enterobacteriaceae family detected. These isolates were characterized as Gram-negative, rod-shaped, and motile microorganisms. The presence of such bacteria raises serious concerns about food safety. It emphasizes the need for improved hygienic practices and rigorous food safety control measures in street food vending areas around the university. Implementing these measures is essential to safeguard student health and maintain the standard of street food quality on campus.
ISOLATION AND SELECTION OF ENDOPHYTE FUNGI FROM THE ANDALIMAN PLANT (Zanthoxylum acanthopodium DC) AS ANTIFUNGAL Idramsa, Idramsa; Ayu, Nur
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14366

Abstract

Dermatophytosis (Ringworm) is a disease caused by dermatophyte fungal infection that attacks the epidermis (keratin) tissue such as skin, nails and hair in humans. Antibiotics as a drug for fungal infections that are often used by the public are known to have side effects, so that alternative drugs are needed that are relatively safe to use. Endophytic microbes found in plants are known to be an alternative natural medicine that does not have side effects, because they contain bioactive compounds. This study aims to isolate, select and identify endophytic fungi in andaliman plants (Zanthoxylum acanthopodium DC) and determine their potential as antifungals against dermatophytosis fungi. Exploration methods were used, molecular analysis using marker genes Internal Transcribed Spacer (ITS) and antifungal activity tests (Dual culture) against Tricophyton rubrum and Tricophyton mentagrophytes as pathogenic test fungi. The results showed that endophytic fungus from the species Diaporthe phaseolorum (Isolate EF128). has very strong antifungal activity against the test fungus Tricophyton rubrum and is strong against Tricophyton mentagrophytes (isolate IJE D3). so it has the potential as an antifungal.
Development of Science Technology Engineering and Mathematics (STEM) Embedded Evaluation Tool for Micology Learning Oetari, Nurul Alfi; Idramsa, Idramsa
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7235

Abstract

Background: STEM learning can help students understand science concepts, improve science skills, create active, creative, motivating learning and provide opportunities for students to apply the material and build their knowledge. This research aims to develop valid and reliable science, technology, engineering and mathematics-based student evaluation tools on fungi material. Methodology: The evaluation instrument was developed using the 4D development model, which includes four stage: Define, Design, Develop and Determine. The study involved 30 first-grade students from MAS Madinatussalam, North Sumatra. The developed evaluation tool consisted of 40 questions. Validation by industry experts confirmed the instrument’s validity and reliability. Findings: Expert evaluations of the questions indicated high suitability, with an average score of 96.75%. Material experts rated the instrument with a suitability score of 92.5%, while media experts gave an average score of 86.1%, all falling within the "very good" category. Reliability testing using Cronbach's Alpha yielded a score of 0.886, indicating high reliability. Based on these results, the STEM-based evaluation tool is considered both valid and reliable for classroom use. Contribution: Expected to provide information as a basis for consideration, supporting the improvement of problem solving skills, increasing critical and creative thinking skills
Co-Authors Adelia Febriyossa Adelia, Ichlasul Anak Agung Istri Sri Wiadnyani Anggraini, Jihan Indah Ashar Hasairin Atmaja, Wia Purwa Ayu, Nur Batubara, Wardah Arijah Batubara, Zihan Zahriani Br Tambunan, Mei Cristina Dwi Apriliani, Dwi Eko Prasetya, Eko Fauziyah Harahap Febriani, Jesika Gultom, Josephine Olivia Hikmal Romadhon, Galih Laksono Trisnantoro Larasati Arum Utami Liliana, Najwa Maharani, Maura Nadeak, Santaro Marito Nasution, Rifka Khairunnisa Naufal, Waliid Oetari, Nurul Alfi Pane, Nita Maya Sari Putri Andini, Putri Rahma, Aisyah Ramadhani, Gita Syahri Rindiani, Artika riwayati riwayati Sari Pane, Nita Maya Siahaan, Eliza Sriyuliani Sidabutar, Jeki Siregar, Aidilia Syafitri Siska Dewi, Alfina Sohada, Ummi Syahputri, Yulia Nanda tampubolon, azarya debora Triana, Rennyta Utami, Mega