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All Journal BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Biosaintifika: Journal of Biology & Biology Education BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Biogenerasi Jurnal Sains Agro Jurnal Agroteknologi Merdeka Pasuruan JURNAL KELUARGA SEHAT SEJAHTERA
Idramsa Idramsa, Idramsa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Nursing Public Health Other

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Molecular Identification of Endophytic Fungi from Bark of Raru (Cotylelobium melanoxylon) that Produce the Antibacterial Compounds Hasanah, Uswatun; Riwayati, Riwayati; Idramsa, Idramsa; Prasetya, Eko
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9424

Abstract

The Dipterocarpaceae plant, raru (Cotylelobium melanoxylon) is widespread in Southeast Asia. The bark of raru has been used by local communities in North Sumatera as antidiarrheal drugs due to its antibacterial compounds. The antibacterial activity of the rarus bark is induced by endophytic fungi that live in the region of the bark. This study aimed to identify the endophytic fungi-producer of antibacterial compounds in the bark of raru (C. melanoxylon) by means of molecular analysis. In general, endophytic fungi have the ability to inhibit the growth of pathogenic bacteria. Thirty-eight isolates of endophytic fungi were isolated from the bark of raru. Selection of isolates for antibacterial activity against Escherichia coli ATCC 35218 and Staphylococcus aureus ATCC 25923 used the dual culture assay. Selection using the dual culture assay yielded 6 endophytic fungal isolates that have the ability to inhibit the growth of test bacteria. EF10A sample was the most powerful isolate inhibiting the growth of both bacteria test. Those six bacteria molecularly identified used a sequence generated from ITS rDNA region. Based on rDNA ITS region sequences, isolate, the producers of the antibacterial compound were identified as Talaromyces cellulolyticus, Penicillium purpurogenum, Aspergillus sp., Trichoderma harzianum, and Aspergillus orizae. The results of this study can be used by researchers to explore more potential endophytic fungi in raru plants (C. melanoxylon) as a source of medicine. The data obtained need to be supported by further research to isolate the bioactive compounds that can inhibit the growth of microbial pathogens.
Pengembangan Buku Nonteks Berbasis Riset Karakterisasi Salak Padangsidimpuan (Salacca Sumatrana Becc.) Rahma, Aisyah; Hasairin, Ashar; Idramsa, Idramsa
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13462

Abstract

This study aims to produce a non-textbook product about the development of a non-textbook based on research into the characterization of Salak Padangsidimpuan (Salacca Sumatrana Becc.). This study uses research and development (R&D) methods with the 4D Thiagarajan development model, which includes the stages of definition, design, development, and dissemination. The results of the study indicate that product validation by content experts obtained a score of 89.1%, categorized as "very feasible." Language experts gave a score of 81%, also categorized as "very feasible," while learning design experts assessed the product with a score of 92.3%, falling into the same category. Feedback from biology lecturers showed that the book is suitable for use, with an average score of 88.9%, classified as "very feasible." In user trials, individual trials resulted in a score of 81.9%, small group trials 82.6%, and limited group trials reached 82.2%, all in the "very feasible category”. In conclusion, the developed non-textbook is highly suitable for use as a reference in teaching spermatophyte taxonomy for biology students. Keywords: Non-textbook, Padangsidimpuan, Development, Salacca Sumatrana
Efektivitas Ecoenzyme Limbah Kulit Jeruk Sebagai Pupuk Organik Cair Terhadap Pertumbuhan Vegetatif Bayam Hijau (Amaranthus hybridus) Siahaan, Eliza Sriyuliani; Siregar, Aidilia Syafitri; Anggraini, Jihan Indah; Br Tambunan, Mei Cristina; Triana, Rennyta; Atmaja, Wia Purwa; Syahputri, Yulia Nanda; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Sains Agro Vol 10, No 1 (2025): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v10i1.1743

Abstract

Ecoenzyme merupakan hasil fermentasi limbah organik, gula, dan air yang mengandung enzim aktif, asam organik, serta unsur hara penting seperti nitrogen, fosfor, dan kalium. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ecoenzyme limbah kulit jeruk sebagai pupuk organik cair (POC) terhadap pertumbuhan bayam hijau (Amaranthus hybridus), serta menentukan konsentrasi yang paling optimal. Penelitian dilaksanakan di rumah kaca menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL), terdiri dari empat perlakuan yaitu kontrol (P0), P1 (1 ml ecoenzym + 200 ml air), P2 (1 ml + 400 ml air), dan P3 (1 ml + 600 ml air), masing-masing dengan enam ulangan. Parameter yang diamati meliputi tinggi tanaman, lebar daun, dan jumlah daun. Data dianalisis menggunakan ANOVA dilanjutkan dengan uji LSD taraf 5%. Hasil penelitian menunjukkan bahwa pemberian ecoenzyme berpengaruh signifikan terhadap tinggi tanaman, lebar daun dan jumlah daun. Perlakuan P1 (1 ml + 200 ml air) dan perlakuan P2 (1 ml ecoenzyme dalam 400 ml air) merupakan konsentrasi yang paling optimal dalam meningkatkan pertumbuhan bayam hijau dengan P1 paling efektif untuk tinggi dan lebar daun, serta P1 dan P2 untuk jumlah daun. Penelitian ini mendukung pemanfaatan ecoenzyme limbah kulit jeruk sebagai alternatif POC yang ramah lingkungan dan efisien dalam budidaya tanaman hortikultura
Efektivitas POC Berbasis Limbah Sayuran dan EM4 Terhadap Pertumbuhan Sawi (Brassica Juncea L.) Rindiani, Artika; Ramadhani, Gita Syahri; Sidabutar, Jeki; Liliana, Najwa; Sari Pane, Nita Maya; Nasution, Rifka Khairunnisa; Batubara, Zihan Zahriani; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Sains Agro Vol 10, No 1 (2025): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v10i1.1749

Abstract

Permasalahan limbah organik rumah tangga dan pasar tradisional yang tidak terkelola dengan baik dapat menimbulkan dampak negatif terhadap lingkungan. Salah satu solusi pemanfaatan limbah tersebut adalah melalui produksi pupuk organik cair (POC) yang ramah lingkungan. Penelitian ini bertujuan untuk mengevaluasi efektivitas pupuk organik cair yang dibuat dari kombinasi limbah sayuran, kulit nanas, dan air cucian beras dengan penambahan Effective Microorganisme 4 (EM4) terhadap pertumbuhan tanaman sawi manis (Brassica juncea L.). Penelitian dilaksanakan secara eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat perlakuan dosis POC: 0 ml (kontrol), 5 ml, 10 ml, dan 15 ml per 250 ml air, masing-masing dengan empat ulangan. Parameter yang diamati meliputi tinggi tanaman, jumlah daun, lebar daun, dan berat basah tanaman. Hasil penelitian menunjukkan bahwa pemberian POC secara signifikan meningkatkan pertumbuhan sawi manis pada semua parameter dibandingkan kontrol, dengan dosis optimal sebesar 15 ml POC+250 ml air. Kandungan hara makro POC, khususnya kalium (0,96%), serta kontribusi bioaktif dari bahan organik dan EM4, berperan dalam merangsang pertumbuhan vegetatif tanaman. Temuan ini menunjukkan bahwa pemanfaatan limbah organik menjadi POC tidak hanya mendukung pertanian berkelanjutan, tetapi juga menawarkan alternatif efektif dalam pengelolaan sampah organik
Pengaruh Pemberian Pupuk Organik Cair (POC) Terhadap Pertumbuhan Tanaman Bayam Merah (Amaranthus tricolor L.) Adelia, Ichlasul; Apriliani, Dwi; Sohada, Ummi; Febriani, Jesika; Batubara, Wardah Arijah; tampubolon, azarya debora; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Agroteknologi Merdeka Pasuruan Vol 9, No 1 (2025): JUNI 2025
Publisher : Universitas Merdeka Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51213/jamp.v9i1.127

Abstract

Penggunaan pupuk kimia yang berlebihan dalam pertanian modern menimbulkan dampak negatif terhadap kesuburan tanah dan lingkungan, sehingga diperlukan alternatif ramah lingkungan seperti pupuk organik cair (POC). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian POC dari nasi basi terhadap pertumbuhan tanaman bayam merah (Amaranthus tricolor L.) serta menentukan konsentrasi POC yang paling optimal. Metode penelitian yang digunakan adalah eksperimen dengan pendekatan deskriptif kuantitatif, yang dilaksanakan selama 12 hari di Rumah Kaca FMIPA Universitas Negeri Medan. Empat perlakuan konsentrasi POC diterapkan (0%, 15%, 25%, dan 35%) dengan parameter yang diamati meliputi tinggi tanaman, jumlah daun, dan panjang akar. Keunikan penelitian ini terletak pada pemanfaatan nasi basi sebagai bahan dasar POC yang masih jarang dikaji pada budidaya bayam merah. Hasil penelitian menunjukkan bahwa konsentrasi 35% memberikan hasil paling optimal dengan rata-rata tinggi tanaman 10,2 cm dan panjang akar 2,3 cm, dibandingkan kontrol yang hanya mencapai 5,8 cm dan 1,1 cm. Namun, jumlah daun tidak menunjukkan perbedaan signifikan di antara perlakuan. Hasil ini menunjukkan bahwa senyawa fermentasi nasi basi seperti asam amino dan enzim mendukung peningkatan pertumbuhan tanaman. Disimpulkan bahwa POC dari nasi basi berpotensi sebagai pupuk alternatif yang ramah lingkungan dan efektif. Disarankan penelitian lanjutan dilakukan dengan uji mikrobiologis untuk mengidentifikasi mikroba dominan dalam POC.
Efektivitas Pemberian Pupuk Organik Cair (POC) dari Limbah Kulit Pisang Kepok dan Kulit Mangga Terhadap Pertumbuhan Kangkung Darat (Ipomoea reptans) Utami, Mega; Siska Dewi, Alfina; Hikmal Romadhon, Galih; Maharani, Maura; Andini, Putri; Naufal, Waliid; Idramsa, Idramsa; Febriyossa, Adelia
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6291

Abstract

The excessive application of inorganic fertilizers in agriculture leads to environmental degradation, necessitating sustainable alternatives. This study evaluated the effectiveness of liquid organic fertilizer (LOF) formulated from banana peel, mango peel, and rice washing water on the vegetative growth of Ipomoea reptans. The research employed a Completely Randomized Design (CRD) with four treatment concentrations: 0 ml/L (control), 10 ml/L, 20 ml/L, and 30 ml/L, each replicated three times. Growth parameters assessed at 21 days after planting included plant height, number of leaves, number of roots, and fresh weight. Laboratory analysis revealed nutrient content of 0.04% nitrogen, 0.17% phosphorus, and 3.04% potassium, indicating a potassium-dominant formulation. The 10 ml/L treatment resulted in the highest plant height (18.00 cm), while the 30 ml/L treatment yielded the highest leaf number (9.33) and fresh weight (0.47 g). However, one-way ANOVA showed no statistically significant differences (p > 0.05) among treatments. The novelty of this research lies in utilizing a combination of commonly discarded household organic waste as a low-cost biofertilizer with measurable nutrient content. Although short-term results were statistically insignificant, the formulation demonstrates potential for refinement and longer application in sustainable agriculture
ISOLATION AND SELECTION OF ENDOPHYTE FUNGI FROM THE ANDALIMAN PLANT (Zanthoxylum acanthopodium DC) AS ANTIFUNGAL Idramsa, Idramsa; Ayu, Nur
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14366

Abstract

Dermatophytosis (Ringworm) is a disease caused by dermatophyte fungal infection that attacks the epidermis (keratin) tissue such as skin, nails and hair in humans. Antibiotics as a drug for fungal infections that are often used by the public are known to have side effects, so that alternative drugs are needed that are relatively safe to use. Endophytic microbes found in plants are known to be an alternative natural medicine that does not have side effects, because they contain bioactive compounds. This study aims to isolate, select and identify endophytic fungi in andaliman plants (Zanthoxylum acanthopodium DC) and determine their potential as antifungals against dermatophytosis fungi. Exploration methods were used, molecular analysis using marker genes Internal Transcribed Spacer (ITS) and antifungal activity tests (Dual culture) against Tricophyton rubrum and Tricophyton mentagrophytes as pathogenic test fungi. The results showed that endophytic fungus from the species Diaporthe phaseolorum (Isolate EF128). has very strong antifungal activity against the test fungus Tricophyton rubrum and is strong against Tricophyton mentagrophytes (isolate IJE D3). so it has the potential as an antifungal.
PERAN SISTEMATIKA MIKROBIA DALAM MENGUNGKAP KEANEKARAGAMAN MIKROORGANISME Idramsa, Idramsa
JURNAL KELUARGA SEHAT SEJAHTERA Vol 11 No 2 (2013): JURNAL KELUARGA SEHAT SEJAHTERA
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jkss.v11i22.3564

Abstract

ABSTRACTDiversity of bacteria and archaea  can not be learned completely   yet not identified perfectly. This is due to the abundant amount of diversity of microorganisms. The existence of molecular technologies allow to identify quickly and accurately the organism. Classification approach is based on the value of character and phylogenetic fenetik make a meaningful classification of microorganisms. Microbes such as bacteria systematic approach should be based on systematic polyfasik. Kata Kunci : Klasifikasi, fenetik, filogenetik, sistematika mikrobia
DESKRIPSI JAMUR ENDOFIT DARI TUMBUHAN RARU (Cotylelobium melanoxylon) GENUS BOTRYTIS Hasanah, Uswatun; Idramsa, Idramsa
JURNAL KELUARGA SEHAT SEJAHTERA Vol 15 No 1 (2017): JURNAL KELUARGA SEHAT SEJAHTERA
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jkss.v15i29.7171

Abstract

ABSTRACK This study aims to identify the endophytic fungus from the bark of the plant raru (Cotylelobium melanoxylon). This study uses identification technique manually by viewing and comparing the macroscopic and microscopic morphological characteristics of each isolate endophytic fungi then seek to obtain dendogram similarity index which divides each isolate into several clusters/genus. Data were analyzed using cluster analysis Simple Matching Coefficient in the Multi Variate Statistical Package (MVSP) where each isolate that had ≥70 % similarity indices are grouped into one cluster/genus. The results of cluster analysis to get 8 cluster/genus endophytic fungi found in the bark of plants raru (Cotylelobium melanoxylon). Eighth genus included the genus Alternaria, Aspergillus, Botrytis, Debaromyces, Fusarium, Mycelia sterile, Nigrospora, and Scopulariopsis. This paper presents a description of the genus Botrytis. Kata Kunci: Identifikasi, Jamur Endofit, Cotylelobium Malanoxylon,  Indeks Similiritas
Co-Authors Adelia Febriyossa Adelia, Ichlasul Anak Agung Istri Sri Wiadnyani Anggraini, Jihan Indah Ashar Hasairin Atmaja, Wia Purwa Ayu, Nur Batubara, Wardah Arijah Batubara, Zihan Zahriani Br Tambunan, Mei Cristina Dwi Apriliani, Dwi Eko Prasetya, Eko Febriani, Jesika Hikmal Romadhon, Galih Laksono Trisnantoro Liliana, Najwa Maharani, Maura Nasution, Rifka Khairunnisa Naufal, Waliid Putri Andini, Putri Rahma, Aisyah Ramadhani, Gita Syahri Rindiani, Artika riwayati riwayati Sari Pane, Nita Maya Siahaan, Eliza Sriyuliani Sidabutar, Jeki Siregar, Aidilia Syafitri Siska Dewi, Alfina Sohada, Ummi Syahputri, Yulia Nanda tampubolon, azarya debora Triana, Rennyta Uswatun Hasanah Utami, Mega