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Antibiofilm activity of Cyanobacteria spirulina as an irrigation solution against Enterococcus faecalis Soesilo, Diana; Pangabdian, Fani; Juniar, Eriza
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.78246

Abstract

Sodium hypochlorite (NaOCl) is currently the golden standard for root canal irrigation. NaOCl at a concentration of 5.25% to 6% can eliminate E. faecalis, but this concentration can increase the risk of toxic effects. Cyanobacteria spirulina is known to produce several secondary metabolites that have antimicrobial activity against gram-positive and gram-negative bacteria. The aim of this study was to determine the antibiofilm power of Cyanobacteria spirulina against the biofilms of Enterococcus faecalis at concentrations of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml. This research was a true experiment with a post-test only group design. The object of the research was divided into 6 groups. Group 1 was a negative control group, group 2 was a positive control group with 5% NaOCl, group 3, 4, 5, 6 were treatment groups given Cyanobacteria spirulina solution at a concentration of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml, respectively. Optical density (OD) of bacteria was bound by staining and analyzed by ELISA auto reader with a wave length of 595 nm (OD 595 nm). The results of the LSD test showed that the significance between K+ and P1, P2, P3 (p< 0.005), K+ and P4 (p= 0.129), P1 and P2 (p=0.449), P3 and P4 (p=0.178). Significance of p<0.0005 showed a significant difference between the groups. The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Least Significance Different (LSD) Post Hoc test method. Cyanobacteria spirulina solution at a concentration of 90 mg/ml had the greatest inhibitory effect on the biofilm formation of Enterococcus faecalis.
Sitotoksisitas komposit serat selulosa sabut kelapa sebagai kandidat novelty basis pada material kedokteran gigi: studi eksperimental cevanti, Twi agnita; Soesilo, Diana; Pangabdian, Fani; Wijaya, Yongki Hadinata; Puspita, Sinta; Hollanda, Ghita Hadi
Padjadjaran Journal of Dental Researchers and Students Vol 7, No 2 (2023): Juni 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v7i2.46092

Abstract

ABSTRAKPendahuluan: Teknologi Fiber-reinforced composite (FRC) sedang dikembangkan sebagai bahan inovatif dalam kedokteran gigi. Menipisnya sumber daya fosil merupakan masalah kritis untuk resin komposit serat saat ini. Sabut kelapa Cocos nucifera L.(coir) memiliki potensi tinggi untuk menggantikan bahan penguat serat sintetis sebagai bahan baru yang akan dikembangkan. Tujuan penelitian mengetahui toksisitas komposit serat selulosa coir sebagai material basis. Metode: Jenis penelitian eksperimental post-test only control group. Selulosa dari coir disintesis menggunakan organosolvent, di bleaching dengan peroxide dalam larutan alkali, serta di nukleasi dengan etanol absolut. Dilakukan uji viabilitas sel fibroblas GT1 untuk serat selulosa kemudian pembuatan komposit selulosa coir dengan fraksi berat 70% filler serat selulosa, dan 30% matriks BisGMA, TEGDMA dan DGEBA. Selanjutnya komposit selulosa coir dilakukan uji toksisitas dari hasil rendaman komposit pada saliva buatan selama 7,14, dan 21 hari menggunakan sel fibroblas BHK-21. Hasil: Hasil uji Post Hoc LSD memberikan kesimpulan bahwa kelompok P-21 didapatkan perbedaan yang signifikan antara kelompok perlakuan K.Sel, P-7, P-14 dengan nilai p<0,05. Dosis paling aman serat selulosa coir sebesar 12,5mg/ml; hasil uji Kruskal-wallis dari lama perendaman komposit terdapat perbedaan yang bermakna antar kelompok dengan nilai signifikansi p<0,05 (p=0,001). Simpulan: Serat selulosa coir tidak toksik sehingga dapat menjadi filler komposit sebagai salah satu prasyarat untuk menjadi kandidat novelty basis pada material komposit kedokteran gigi.KATA KUNCI: coir, komposit, toksistas, basis kedokteran gigiCytotoxicity of cellulose fiber from coconut coir as a novelty base candidate on dental materials: experimental studyABSTRACTIntroduction: Fiber-reinforced composite (FRC) technology is being developed as an innovative material in dentistry. The depletion of fossil resources is a critical issue for current fiber composite resins. Cocos nucifera L. coconut fiber (coir) has high potential to replace synthetic fiber reinforcement as a new material to be developed. The aim of the study was to determine the toxicity of coir cellulose fiber composites as a base material. Methods: This was a post-test only control group experimental study. Cellulose from coir was synthesized using organosolvent, bleached with peroxide in alkaline solution, and nucleated with absolute ethanol. GT1 fibroblast cell viability test was conducted for cellulose fibers and then coir cellulose composites were made with a weight fraction of 70% cellulose fiber filler, and 30% BisGMA, TEGDMA and DGEBA matrix. Furthermore, cellulose coir composites were tested for toxicity from the results of composite immersion in artificial saliva for 7, 14, and 21 days using BHK-21 fibroblast cells. Results: The results of the Post Hoc LSD test concluded that the P-21 group had a significant difference between the K.Sel, P-7, P-14 treatment groups with a value of p<0.05.The safest dose of coir cellulose fiber is 12.5mg/ml; the results of the Kruskal-wallis test of the composite soaking time have significant differences between groups with a significance value of p<0.05 (p=0.001). Conclusion: Coir cellulose fiber is non-toxic so it can be a composite filler as one of the prerequisites to be a novelty base candidate in dental composite materials.KEY WORDS: coir, composite, toxicity, dental base
STRATEGI INOVATIF DALAM MANAJEMEN SUMBER DAYA MANUSIA RUMAH SAKIT BHAYANGKARA WAHYU TETUKO BOJONEGORO: ANALISIS STUDI KASUS PADA IMPLEMENTASI PRAKTIK TERBAIK UNTUK PENINGKATAN EFEKTIVITAS ORGANISASI Arini, Virginnita Whindi; Yuniarta, Alfian; Soesilo, Diana; Nurrahim, Firman; Wahyu Setiani, Muhammad; Fitricia, Ria; Amelia, Ira
Jurnal Medika Malahayati Vol 8, No 1 (2024): Volume 8 Nomor 1
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jmm.v8i1.13258

Abstract

Abstrak: Strategi Inovatif dalam Manajemen Sumber Daya Manusia Rumah Sakit Bhayangkara Wahyu Tetuko Bojonegoro: Analisis Studi Kasus pada Implementasi Praktik Terbaik untuk Peningkatan Efektivitas Organisasi. Penelitian ini bertujuan untuk mengeksplorasi strategi inovatif dalam manajemen SDM rumah sakit, dengan memfokuskan pada analisis studi kasus implementasi praktik terbaik. Metode penelitian menggunakan pendekatan kualitatif dengan studi kasus pada Rumah Sakit Bhayangkara Wahyu Tetuko Bojonegoro. Data dikumpulkan melalui wawancara mendalam dengan pimpinan dan praktisi SDM, observasi, dan analisis dokumen terkait. Hasil penelitian menunjukkan bahwa rumah sakit telah menerapkan strategi inovatif, termasuk penggunaan teknologi terkini, pengembangan keterampilan karyawan, promosi budaya kolaboratif, dan peningkatan proses rekrutmen. Penelitian ini menyimpulkan bahwa strategi inovatif, terutama melalui penerapan praktik terbaik, mampu menjadi kunci untuk meningkatkan efektivitas manajemen SDM di rumah sakit. Temuan ini dapat menjadi pedoman berharga bagi rumah sakit dan institusi kesehatan serupa dalam mengembangkan model manajemen SDM yang responsif dan adaptif. Implikasi dari penelitian ini dapat memberikan kontribusi positif terhadap kemajuan pelayanan kesehatan secara keseluruhan.
Antimicrobial activity of calcium hydroxide, calcium oxide, and mineral trioxide aggregate paste against α-Hemolytic Streptococcus Darmadi, Eveline Yulia; Soesilo, Diana
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.24-28

Abstract

Background: The bacteria mostly found in the root canals are aerobic and facultative anaerobic bacteria, such as α-Hemolytic Streptococcus. Calcium hydroxide has alkaline characteristics with pH of 12.5, and antimicrobial properties. Calcium oxide is hydrophilic and has a smaller molecular weight than calcium hydroxide. Mineral trioxide aggregate (MTA) has alkaline properties, good biocompatibility, stimulates healing and inhibits bacterial growth. Purpose: The purpose of this study was to examine the differences of antimicrobial activity between calcium hydroxide, calcium oxide, and mineral trioxide aggregate (MTA), also to find which of these materials has the highest antimicrobial activity. Methods: The design of this study was a post-test only control group design. The study subjects were divided into 4 groups. K (-) was control group without any treatment, P1 was treated with calcium hydroxide, P2 was treated with calcium oxide, and P3 was treated with MTA. Each group consisted of 7 samples. The α-Hemolytic Streptococcus were spread on Mueller Hinton agar. Antimicrobial test was using diffusion method and diameter of inhibition zones were measured with caliper. Results: Average inhibitory zone of each sample was P1 (30.9643 mm ± 0,7431), P2 (35,2357 mm ± 0,7099), P3 (28,6 mm ± 1,5532). Tukey HSD test showed significances results between samples P1 and P2 (p=0,001), P1 and P3 (p=0,002), P2 and P3 (p=0,001). Conclusion: The highest antimicrobial activity against α-Hemolytic Streptococcus was calcium oxide followed by calcium hydroxide and MTA.
Pengaruh Metode Pencampuran Coupling Agent Terhadap Sitotoksisitas Komposit Serat Selulosa Sabut Kelapa / Coir Cevanti, Twi Agnita; Prananingrum, Widyasri; Soesilo, Diana
Jurnal Material Kedokteran Gigi Vol 11 No 1 (2022): JMKG Vol 11 No 1 Maret 2022
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v11i1.1032

Abstract

Research about cellulose fiber from coconut fibers (Cocos nucifera L/coir) was currently done as an organic fillers for dental composite materials. DGEBA was chosen as a coupling agent to obtain covalent chemical bonds between BisGMA, TEDGMA matrices and coir cellulose fibers. The mixing method of DGEBA played an important role in the biocompatibility of the material. The ideal requirement for dental materials must be non-toxic through cytotoxicity tests. Objective to modify the synthesis method of coir-based cellulose as a natural fiber to optimize chemical bonding through polymerization reaction between fiber, coupling agent and matrix. Methods: There were 2 kinds of cellulose synthesis methods, which were (1) synthesis of cellulose solution using DGEBA and ethanol, (2) synthesis of cellulose solution using ethanol without DGEBA. Cytotoxicity test on pulp fibroblast cells with MTT Assay method on coir cellulose fiber composite samples from the two methods. Method 1 cell viability rate for 7 days immersion was 93.30855%, 14 days was 99.07063%, 21 days was 74.53532%. Method 2 obtained 64.68401% for 7 days immersion, 73.79182% for 14 days and 53.15985% for 21 days. Conclusion, the cellulose solution synthesis method using DGEBA and ethanol produces composites with better chemical bonds seen from the results of the cytotoxicity test
Pendidikan Dan Pelatihan Kader Sebagai Salah Satu Pilar Penting Perubahan Perilaku Di Bidang Kesehatan Gigi Dan Mulut Pranoto, Amelia Elizabeth; Aprilia, Aprilia; Rahmitasari, Fitria; Soesilo, Diana; Puspita, Sinta; Riski, Mardiyanti; Ariestania, Vivin; Lestari, IGAM Oka; Raharjo, Pambudi; Wijaya, Yongki Hadinata; Rizal, Mohammad Basroni; Sarianoferni, Sarianoferni; Hendro, Onge Victoria; Wira, Rizko; Sularsih, Sularsih; Cahyo, Benny Dwi; Paramita, Putri
Jurnal Pengabdian Masyarakat Bangsa Vol. 2 No. 11 (2025): Januari
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v2i11.1930

Abstract

Lima dari sepuluh anak-anak Indonesia usia sekolah dasar memiliki masalah gigi berlubang. Hal ini disebabkan oleh kebersihan rongga mulut yang buruk dan kurangnya pengetahuan anak-anak terkait pentingnya menjaga kesehatan gigi dan mulut. Anak-anak juga seringkali tidak menyikat gigi di waktu yang benar, ditambah lagi cara menyikat gigi yang keliru menjadi salah satu faktor munculnya gigi berlubang. Dokter gigi dari Fakultas Kedokteran Gigi, Universitas Hang Tuah Surabaya melakukan kegiatan pengabdian masyarakat berupa School Health Program – Training of Trainer (SHP-ToT). Kegiatan ini bertujuan untuk menciptakan kader kesehatan gigi dan mulut yang berasal dari lingkungan internal sekolah. Diharapkan kader-kader tersebut dapat menjadi pilar penggerak utama pada pendidikan anak-anak usia sekolah dasar akan pentingnya menjaga kebersihan gigi dan mulut secara berkelanjutan. Kader akan diberi pelatihan dan diuji sejauh mana pemahamannya terkait kesehatan gigi dan mulut. Setelah pelatihan selesai, kader akan terus dipandu untuk mengajarkan anak-anak sekolah dasar tentang pentingnya menjaga kesehatan rongga mulut sekaligus menjadi supervisor anak-anak agar mau menyikat gigi pagi dan malam selama 21 hari berturut-turut.
Effectiveness of Nipah leaf extract (Nypa fruticans) against Streptococcus mutans biofilm as cavity cleanser Handriutomo, Yosef Kevin; Soesilo, Diana; Aprilia, Aprilia; Parisihni, Kristanti; Rayhan, Rafialdi; Cahyani, Febriastuti
Conservative Dentistry Journal Vol. 15 No. 1 (2025): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i1.2025.23-26

Abstract

Background: Streptococcus mutans is the primary bacterium responsible for dental caries, found in the biofilm on the tooth surface, known as dental plaque. Before restorative treatment or cavity care for carious teeth, a cavity cleanser is necessary to remove residual bacteria that could cause secondary caries or restoration failure. Nipah leaf extract contains phenol, flavonoids, saponins, and steroids, which can destroy bacteria by denaturing proteins and damaging bacterial cell membranes. Objective: To determine the antibiofilm activity of Nipah leaf extract against Streptococcus mutans biofilm. Materials and Methods: The materials used were Nypa fruticans leaf extract prepared using the maceration method at concentrations of 1%, 5%, 10%, and 15%, with chlorhexidine gluconate as the positive control and distilled water as the negative control. Bacteria cultured in BHI media were inoculated into a 96-well flat-bottomed plastic tissue culture plate and incubated for 24 hours at 37°C. Optical Density (OD) was measured using an ELISA reader. Results: KM: 0.039; K (-): 0.887; K (+): 0.085; P1: 0.727; P2: 0.463; P3: 0.347; P4: 0.169. The Mann-Whitney test results showed significant differences between K (+) and P1 (0.000), K (+) and P2 (0.000), K (+) and P3 (0.000), K (+) and P4 (0.005), P1 and P2 (0.000), P1 and P3 (0.000), and P1 and P4 (0.000). The results were significant as p < 0.05. Conclusion: Nipah leaf extract (Nypa fruticans) can inhibit the biofilm formation of Streptococcus mutans bacteria.
PENGABDIAN MASYARAKAT PEMBERIAN EDUKASI KESEHATAN GIGI DAN MULUT PADA SISWA SD HANG TUAH 1 SURABAYA Soesilo, Diana; Brahmanta, Arya; Ramadhi, Cakrawartyha; Hermanto, Eddy; Khoironi, Emy; Wijaya, Yongki Hadinata; Fitriani, Yufita; Syahdinda, Meralda Rossy
BESIRU : Jurnal Pengabdian Masyarakat Vol. 2 No. 6 (2025): BESIRU : Jurnal Pengabdian Masyarakat, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/besiru.v2i6.1411

Abstract

Latar belakang.  Karies gigi termasuk penyakit infeksi kronis yang biasanya terjadi akibat bakteri kariogenik yang menempel pada gigi yang akan memetabolisme gula sehingga menghasilkan asam, yang seiring dengan waktu akan mendemineralisasi struktur gigi. Anak-anak usia sekolah sangat menyukai makanan dan minuman manis dengan kandungan glukosa tinggi, dan sering kali kurang memahami teknik menyikat gigi yang benar, serta jarang memeriksakan gigi mereka ke fasilitas kesehatan. Metode pelaksanaan. Siswa akan diberikan soal pre test dan post test untuk mengetahui pengetahuannya terhadap kesehatan gigi dan rongga mulut sebelum dan setelah diberikan edukasi dan dilakukan pemeriksaan kondisi rongga mulut siswa oleh  dokter gigi RSGMP Nala Husada. Hasil dan pembahasan. Hasil pre tes dan post tes menunjukkan terdapat 1 orang dari 85 orang siswa yang mengikuti tes memperoleh skor SEMPURNA. Pada kategori NAIK persentasenya adalah 76% yang artinya terdapat 64 orang. Kategori hasil TETAP terdapat 17% berarti terdapat 14 orang yang skornya TETAP. Terdapat 6 orang atau sekitar 8% dari siswa yang nilai post test-nya lebih buruk daripada nilai pre test sehingga termasuk dalam kategori TURUN.Kesimpulan Pemberian edukasi tentang kesehatan gigi dan rongga mulut siswa SD Hang Tuah I memberikan hasil yang baik karena persentase terbesar adalah terdapat peningkatan pengetahuan siswa SD Hang Tuah I tentang kesehatan rongga mulut.
Antifungal effect of calsium hydroxide and cresotin against candida albicans as root canal treatment materials Aprilia, Aprilia; Cevanti, Twi Agnita; Soesilo, Diana; Damaiyanti, Dian Widya; Velisia, Jessica
Padjadjaran Journal of Dentistry Vol 33, No 2 (2021): July 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no2.28252

Abstract

Introduction: Candida albicans can cause quiet high prevalance of root canal treatment failure (35%). Candida albicans are difficult to eliminate and resistant to Calsium hydroxide. Therefore the research was conduct to determinate sterilization material which is effective as antifungal material to eliminate Candida albicans so can minimize the possibility of falure in root canal treatment. Purpose Obtain the optimum concentration of paste that consist of Calsium hydroxide powder combine with Cresatin liquid against the Candida albicans and must be safe to used in root canal tratement medication. Method: The initial examination of antifungal activities was carried out using the disc diffusion with spread plate technique. Medium that used in this method is Medium SGA (Saburoud’s Glucose Agar). Result: The result of the One-way ANOVA test showed a significant value of p= 0,000 (p<0,05). The result of Least Significant Difference test showed that each group had a significant difference because the value of p=0 (p<0,05).  There is significant difference between each group. Conclusion: The most effective as antifungal against Candida albicans is in mixing 1:2 of calsium hydroxide powder and Cresotin liquid which mean 1 full measuring spoon and 2 drops of Cresotin liquid. Mixture of Ca(OH)2 with metacresylacetate produce a chemical reaction namely calsium cresilate and Acetic acid. Calsium cresilate is strong disinfectant. Acetic Acid dissociates and releases hydroxyl ions (H+). Changes in pH cause the transformation of Candida albicans from Y form into M form so it inhibitis cell division. 
PENINGKATAN PENGETAHUAN GURU SDN KEPUTIH 245 SURABAYA MELALUI KEGIATAN TRAINING OF TRAINER Victoria, Onge; Mulawarmanti, Dian; Diyah N.K., Chaterina; Pinansti, Restika Anindya; Artyas Annariswati, Icha; Iman, Dimas; Riski, Mardiyanto; Febrina, Agni; Maharani, Aulia Dwi; Rossy, Meralda; Megantara, Rizko Wira Artha; Yoyada, Novendy; Soesilo, Diana; Hayati, Kurnia; Paramita, Annisa Listya; Rizal, Mohammad Basroni
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i2.111

Abstract

Abstrak: Kesehatan  gigi dan  mulut  di  Indonesia  masih  merupakan  masalah  kesehatan  yang perlu diperhatikan terutama pada anak – anak di usia sekolah dasar. Salah satu upaya untuk mengatasi masalahan kesehatan gigi dan mulut di Indonesia serta partisipasi para dokter gigi dalam menunjang program “Indonesia Bebas Karies 2030”,  khususnya pada kelompok  masyarakat  usia  sekolah  dan  dewasa. Fakultas Kedokteran Gigi Universitas Hang Tuah Surabaya bekerja sama dengan Unilever melakukan   kegiatan promotif – preventif. Kegiatan tersebut dinamai “Training of Trainer”. Program ini dilakukan di SDN Keputih 245 Surabaya dengan beberapa kegiatan yang dilakukan diantaranya yaitu pemberian pelatihan tentang kesehatan gigi dan mulut, cara menyikat gigi yangbenar dan pemantauan terhadap waktu menyikat gigi yang benar.  Hasil akhir dari program ini  diharapkan  dapat  mencegah  meningkatnya  derajat  kesehatan  gigi  dan  mulut  khususnya di SDN Keputih 245 Surabaya.