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All Journal Jurnal Sain Veteriner BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Atik Ratnawati
Balai Besar Penelitian Veteriner Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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DETEKSI VIRUS AVIAN INFLUENZA SUBTIPE H5N1 DI BEBERAPA PASAR UNGGAS HIDUP DALAM WILAYAH PROVINSI JAWA BARAT SEKITARNYA Ratnawati, Atik; Dharmayanti, NLP Indi
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2778

Abstract

Pada penelitian ini dilakukan identifikasi virus avian influenza (AI) subtipe H5N1 pada unggas dan lingkungan pasar untuk mengetahui peran pasar sebagai sumber penularan virus. Metode yang digunakan dalam penelitian ini adalah pengambilan sampel swab kloaka unggas dan lingkungan di beberapa pasar di wilayah Jawa Barat dan Tangerang. Sampel selanjutnya dilakukan isolasi ribonucleic acid (RNA) dan dilakukan reverse transcriptase polymerase chain reaction (RT-PCR) dengan menggunakan primer AI subtipe H5N1. Hasil penelitian menunjukkan bahwa virus AI/H5N1 terdeteksi pada unggas dan lingkungan pasar. Disimpulkan bahwa pasar dapat menjadi sumber penularan virus AI subtipe H5N1 terhadap unggas lainnya.
PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER Sendow, Indrawati; Adjid, R.M. Abdul; Ratnawati, Atik; Saepulloh, Muharam
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2775

Abstract

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.
Identification of Bovine Rotavirus Group A in Bogor, West Java Hewajuli, Dyah Ayu; Pratama, Yuda; Winarsongko, Agus; Purwani, Ani; Suyatno, Teguh; Fabeane, Ajeng; Ermayati, Ermayati; Nuradji, Harimurti; Assadah, Nur Sabiq; Endrawati, Dwi; Ratnawati, Atik; Saepulloh, Muharram; Dharmayanti, NLP Indi
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90683

Abstract

Abstrak  Diare merupakan penyakit yang menyebabkan angka kesakitan yang tinggi pada pedet dan kematian neonatal. Penyakit ini dapat disebabkan oleh beberapa agen penyakit yang berbeda. Rotavirus Grup A (RVA) atau Bovine Rotavirus merupakan salah satu agen infeksi penyebab diare pada pedet. Selanjutnya, diare neonatal pada pedet dapat berdampak pada kerugian ekonomi bagi ternak sapi perah dan sapi potong di seluruh dunia karena menyebabkan gangguan pertumbuhan, meningkatnya biaya perawatan, dan/atau kematian pada hewan sakit. Prevalensi Bovine Rotavirus dapat berbeda antar negara di seluruh dunia. Sirkulasi Bovine Rotavirus pada sapi telah dilaporkan di beberapa negara tetapi sirkulasi Bovine Rotavirus pada sapi di Indonesia belum diketahui. Untuk mengetahui prevalensi Rotavirus grup A atau Bovine Rotavirus (BRV), 100 sampel feses dikoleksi dari pedet dengan gejala klinis diare atau tidak diare di Kabupaten Bogor, Jawa Barat pada tahun 2021. Sampel dianalisis terhadap urutan yang mengkode protein kapsid bagian dalam VP6 (subkelompok) menggunakan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Lima dari 100 sampel feses sapi (5%) terdeteksi positif BRV. Pada penelitian ini menunjukkan bahwa kelompok Rotavirus atau Bovine Rotavirus (BRV) telah bersirkulasi di antara ternak sapi di Indonesia, khususnya Kabupaten Bogor. Sampel positif  Rotavirus grup A atau Bovine Rotavirus (BRV) dapat diidentifikasi dengan metode diagnosis dini (RT-PCR). Kata kunci : Bovine Rotavirus; RT PCR; Bogor Abstract              Diarrhea is the most disease that cause high morbidity in calves and neonatal mortality. This disease can be caused by several different infectious agents. Group A rotaviruses (RVA) or Bovine Rotavirus are one of the infectious agents causing diarrhea in calves. Then, Neonatal calf diarrhea can impact to economic losses to dairy and beef cattle herds worldwide, in consequence of growth disorders, value of treatment, and/or death of sick animals. The prevalence of Bovine Rotavirus can become different in the worldwide. The circulation of these bovine rotavirus in calves from the some region has already been demonstrated but the circulation of bovine rotavirus in Indonesia is not known. To investigate the prevalence of A group Rotaviruses or Bovine Rotavirus (BRV), 100 fecal samples were collected from calves with diarrhea or no diarrhea in Bogor district, West Java at 2021. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) using RT-PCR. Five of 100 specimens of bovine fecal (5%) were detected positive as BRV positive. In this study, A group Rotaviruses or Bovine Rotavirus (BRV) have been circulated among cattle herds in Indonesia, particularly Bogor District. The positive samples of A group Rotaviruses or Bovine Rotavirus (BRV) can be identified using the early diagnosis method (RT-PCR). Keywords : Bovine Rotavirus; RT PCR; Bogor District
IN SILICO ANALYSIS OF SMALL INTERFERING RNA TARGETING THE NUCLEOPROTEIN GENE OF INFLUENZA VIRUSES Hartawan, Risza; Ratnawati, Atik; Sendow, Indrawati; Dharmayanti, Ni Luh Putu Indi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8521

Abstract

Small interfering RNA (siRNA) is a promising therapeutic against viral infection, includ-ing Influenza viruses. However, the Influenza viruses have massive variants with high mutation rates. Therefore, the siRNAs could be futile against newly emerging viruses. Thus, this study aimed to analyze siRNAs targeting the nucleoprotein gene of Influen-za viruses. Using bioinformatic analyses, the siRNAs were simulated against 5 sub-types of Influenza viruses, including H1N1, H3N2, H5N1, H7N9, and H9N2. Bioinfor-matic tools for the folding structure of messenger RNA were utilized to select effective siRNA. As a result, 32 siRNA sequences targeting the nucleoprotein gene were identi-fied. The precision medicine concept seems applied to the siRNA treatment for the In-fluenza virus since each siRNA is effective in its respective virus target. Based on the nucleotide mismatch parameter, most siRNA does not have coverage for the multiple infections of all five subtypes of Influenza viruses, except for NP1089 and NP1496. Later, the secondary and tertiary structure analysis of messenger RNA demonstrated that siRNA has different circumstances in its RNA target position. Therefore, siRNA mapping based on the RNA folding structure approach provides a tool for selecting more effective sequences against Influenza virus infection. Both siRNA NP1089 and NP1496 were predicted to have similar effectivity in knocking down Influenza virus in-fection. Moreover, the cocktail application of siRNA treatment may be effective as an alternative strategy in matching co-infection of multiple Influenza virus subtypes.
Co-Authors Adjid, RM. Abdul Adjid, RM. Abdul Assadah, Nur Sabiq Breed, Andrew Breed, Andrew Daniels, Peter Daniels, Peter Dwi Endrawati Dyah Ayu Hewajuli Ermayati, Ermayati Fabeane, Ajeng Field, Hume Field, Hume Indrawati Sendow Morrissy, Chris. Morrissy, Chris. Muharam Saepulloh NI LUH PUTU INDI DHARMAYANTI NLP I Dharmayanti NLP Indi Dharmayanti Nuradji, Harimurti Purwani, Ani R.M. Abdul Adjid RISA INDRIANI RISZA HARTAWAN Saepullah, Muharam Saepulloh, Muharram Sendow, Indrawati Suyatno, Teguh Winarsongko, Agus yuda pratama