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Pratylenchus brachyurus (Godh) Filipjev. & Stekhoven is one of the importantplantparasitic nematodes that attacked root of patchouli and the causal of mot necrosis, growth retardation, reddish or yellowish leaf: A greenhouse study was conducted to evaluate the level of resistant of six patchouli cultivars, those were wide planted in Sumatera against P. brachyurus, and to determine the effect of population level of the nematodes on three dzrerent resistance patchouli. Six patchouli cultivars were Rina Sriwati; Meity S. Sinaga; Abdul Muin Adnan' Ika Mustika
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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Pratylenchus brachyurus (Godh) Filipjev. & Stekhoven is one of the importantplantparasitic nematodes that attacked root of patchouli and the causal of mot necrosis, growth retardation, reddish or yellowish leaf: A greenhouse study was conducted to evaluate the level of resistant of six patchouli cultivars, those were wide planted in Sumatera against P. brachyurus, and to determine the effect of population level of the nematodes on three dzrerent resistance patchouli. Six patchouli cultivars were inoculated with 200 nematodes& soil. Based on reproduction index the cultivar of Seulimum Putih, Pidie and Sidikalang known as susceptible ones Tapaktuan and Seulimum Merah was moderate resistant and Girilaya was resistant. Furthermore, the effect of population levels of P. brachyurus (0, 100, 200 and 800 nematodes& soil) on three patchouli cultivars showed that 200 nematodeskg soil population caused significant effect on reduced shoot and weight root and plant height on susceptible cultivars, howevec on the moderat resistant and resistant cultivar the signifcance effect occurred at 800 nematodeslkg soil. The reproduction factors on three patchouli cultivars were decreased with the increased of population nematodes level. me oil and chlorophyll content have been decreased on the infectedpatchouli cultivars atfive month afCer inoculation.

KEMAMPUAN BERTAHAN HIDUP TRICHODERMA HARZIANUM DAN TRICHODERMA VIRENS SETELAH DITUMBUHKAN BERSAMA DENGAN JAMUR PATOGEN TULAR TANAH SECARA IN VITRO Rina Sriwati; T. Chamzurni; L. Kemalasari
Jurnal Floratek Vol 9, No 1 (2014): April 2014
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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The experiment was conducted at the Laboratory of Plant Pathology Faculty of Agriculture, Syiah Kuala University from February to June 2013. The purpose of study was to evaluate the survival of T. harzianum and T. virens after cohabiting with soil borne pathogenic fungi (Fusarium sp, Rhigidoporus sp, and Sclerotium sp) as well as the type of interaction. Trichoderma fungi and fungal pathogens were grown on PDA in vitro, and then observed their growth after being reisolated on 3 different growth zones. The results showed that T. harzianum and T. virens growing with Fusarium sp were capable to grow over the space. It was evident from the results of reinoculation on a petridish B (border zone) and C (zone Trichoderma) colonies, that T. harzianum and T. virens regrew, while the pathogens were not able to regrow. When cohabiting with Sclerotium sp and Rhigidoporus sp, antagonistic fungus regrew after reisolation but pathogens also grew on Petridis B (border zone). It indicates that on the contact area (petri dish B), Sclerotium sp and Rhigidoporus sp conduct defense over the competition. Regrowth of fungal pathogens on contact area (border) indicates that the fungus has a high level of competition. Antagonist agents such as T. virens and T. harzianum were able to survive and regrow after cohabiting with fungal pathogens and showed type A interaction (against Fusarium sp), while cohabiting with Sclerotium sp and Rhigidoporus sp showed the type B interaction.

Colonization Ability of Biological Control Agent Tricoderma spp on Cocoa Pod and Seedling Rina Sriwati; Tjut Khamzurni; Elvira Iskandar; Mitra T. A. Lestari
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Some of Trichoderma species as antagonistic fungi are usually considered soil microorganism, They colonize plant roots, some- times forming a symbiotic relationship. Three species of Trichoderma (T. virens, T. harzianum and T. asperellum) have been inoculated on cacao seedling and cocoa pod. Trichoderma species can be re-isolated from surface sterilized cacao seedling, including the stem and leaf, root, and pod then observed their colonization ability. Fungal hyphae were observed under the microscope emerging from the leaf, steam, root of seedling and pod as soon as 1 day after their isolation from surface sterilized cacao seedling and pod. All Trichoderma species were able to enter and make colonization. The highest percentage of colonization occurred in the T. harzianum by 73.3% (leaves), 46.7% (trunk) and 86.7% (roots). While colonization on the skin cocoa pod (epidermis) also has a different percentage, the highest percentage indicated in the treatment of T. harzianum by 63.3%. We conclude that T. harzianum better biological control agent base on their ability to colonize all part of seedling and pod. Trichoderma species into the cacao stem, leaf, root and pod allowing systemic colonization of this tissue.

FORMULATION OF TRICHODERMA VIRENS ORIGIN OF ACEH COCOA CONTROLLING BLACK POD DISEASE CAUSED BY PHYTOPHTORA PALMIVORA TJUT CHAMZURNI; RINA SRIWATI; RIZKY MUARIF; BUNI AMIN; ABDUH ULIM
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Trichoderma virens have been isolated from rooting cocoa in Aceh and in the laboratory have been reported capable of inhibiting the growth of P. palmivora in vitro. However, the formulations of T. virens from Aceh can be used as an alternative product for farmers in controlling black pod disease of cocoa. This research was conducted t the Laboratory of Plant Pathology College of Agriculture Syiah Kuala University and cocoa fruit samples obtained from cocoa plantations owned by farmers in Pidie Jaya District of Aceh. The purpose of the study was to obtain an application formulations strains of T. virensfrom Aceh, as one of the biological control study consisted of seven treatments, namely W (water), Pest (Mancozeb Fungicide), Det (Detergent), Det+Tv( detergent+strainsof T.virens), P+Tv (Adhesive +strainsof T.virens), Co+Tv( Corn Oil+strainsof T.virens), Po+Tv (Palm oil+strainsof T.virens) and each treatment was repeated 10 times to obtain70 experimental units. The parameters observed incubation period, disease incidence (%), and the diameter of the spots (cm). Application results show that the strains of T. virens Origin Aceh effective in controlling P. palmivora on cocoa pods and the best formulation in controlling P. palmivora on cocoa pods are corn oil+T. virens formulation (Co +Tv) compared with other formulations are characterized by the length of the incubation period, the low incidence of P. palmivora disease, and the small diameter of the spots on the cocoa pods.

Fig Pollinating Wasp Transfers Nematodes into Figs of Ficus racemosa in Sumatra, Indonesia Jauharlina Jauharlina; Rina Sriwati; . Yusmaini; Natsumi Kanzaki; Stephen Compton
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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The fruits (figs) of fig trees (Ficus spp, known as ‘bak ara’ in Aceh), are the source of food for many species of faunas in the forest, including birds, monkeys, orangutans, etc. Pollination within the figs totally depends on female fig wasps that belong to family Agaonidae. Fig trees and their pollinating wasps rely on each other to survive. Female fig wasps are known to transport nematodes into receptive figs when the wasps enter the figs to lay eggs. An investigation on the nematodes carried by female pollinating wasps Ceratosolen fusciceps Mayr into figs of Ficus racemosa was conducted in Sumatra, Indonesia. The figs on the trees were regularly sampled to determine the presence of nematodes and infer their ecology. The Baermann funnel method was employed to extract the nematodes from the figs. Eight species of nematodes were recorded from the figs, two of which are still unidentified. The species found were (1) Teratodiplogaster fignewmani, (2) Teratodiplogaster sp., (3) Parasitodiplogaster sp., (4) Schistonchus sp1., (5) Schistonchus sp2., (6) Mononchoides sp., (7) and (8) two undescribed Diplogastridae species (‘umbrella-like’ species 1 and species 2). This is the most diverse fig nematode community recorded. The highest nematode populations were routinely found in D-phase figs, when the new generations of wasps were about to emerge. Details of the ecology of each nematode species are likely to differ, but as a group they did not seem to significantly affect seed and wasp development in F. racemosa figs

The effect of intercropping and different dose of goat manure application on growth of Pogestemon cablin Benth Rivan Rinaldi; Indra Indra; Muhammad Syaifullah; Rina Sriwati; Bob Douma; Zulfahrizal Zulfahrizal; Ulfa Hansri Ar Rasyid
Journal of Patchouli and Essential Oil Products JOURNAL OF PACTHOULI AND ESSENTIAL OIL PRODUCT : VOLUME 1, ISSUE 1 (JUNE 2022)
Publisher : Universitas Syiah Kuala

Patchouli (Pogostemon cablin Benth) is a perennial aromatic crop that can produceessential oil which is generally used as a mixture of ingredients for making cosmetics, pharmaceuticals, and aromatherapy which functions as a binder/fixative agent and pharmaceuticals. Although the production of patchouli increases year to year, the productivity per hectare of this plant showed a decreasing pattern. Efforts are needed to increase the productivity of patchouli, such as improving seeds quality and applying best management practices in planting. This study aimed to examine the optimum application of manure doses and intercropping practice on maximum growth of patchouli at first and two months after planting. The RCD (Randomize Complete Design) was conducted by using a cropping system and the application of goat manure in different doses. There were two levels of the cropping system: monocropping and intercropping with mung beans (Phaseolus radiatus) and four-level of manure in different doses were applied, 0 g, 500 g, 1000 g and 2000 g. ANOVA analysis shows that no interaction between cropping system and manure application on the growth of patchouli. All variables of patchouli growth in monocropping are better than in intercropping at two months after transplanting. Furthermore, there was no effect of manure application on canopy width, fresh and dry biomass of patchouli. The effect is only found on plant height and tree basal diameter, while without manure application and 500 g application is greater than 1000 g and 2000 g of manure application.

Aplikasi Formulasi Cair Trichoderma harzianum dan Trichoderma virens sebagai Agen Pengendali Hayati (APH) Penyakit Hawar Daun (Phytopthora palmivora) pada Bibit Kakao Desi Arida; Rina Sriwati; Tjut Chamzurni
Jurnal Ilmiah Mahasiswa Pertanian Vol 4, No 2 (2019): Mei 2019
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Abstrak. Pencegahan penyakit hawar daun oleh jamur Phytophthora palmivora pada kakao (Theobroma cacao L.) menggunakan fungisida dapat berdampak buruk terhadap lingkungan dan menimbulkan resistensi, sehingga untuk mengatasinya diperlukan alternatif lain dengan menggunakan Agen Antagonis Hayati (APH) seperti cendawan Trichoderma spp. Perbanyakan cendawan Trichoderma spp. dapat diperbanyak dengan berbagai media, salah satunya dengan formulasi cair. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi dari T. harzianum dan T.virens dalam mengendalikan penyakit hawar daun P. palmivora pada bibit kakao. Penelitian ini dilaksanakan di Laboratorium Penyakit Tumbuhan dan Kebun Percobaan Fakultas Pertanian Universitas Syiah Kuala pada bulan Januari 2018 sampai dengan Juli 2018. Penelitian ini menggunakan Rancangan Acak Lengkap non faktorial yang terdiri dari 4 perlakuan dengan 5 ulangan, Apabila uji F menunjukkan pengaruh nyata, dilanjutkan dengan uji beda nyata terkecil pada taraf 5%. Peubah yang diamati yaitu masa inkubasi, kejadian penyakit, keparahan penyakit jumlah daun, lebar daun, diameter batang dan tinggi tanaman. Hasil penelitian ini menunjukkan aplikasi formulasi cair T. harzianum dan T. virens berpengaruh terhadap peubah masa inkubasi, kejadian penyakit, keparahan penyakit dan tinggi tanaman yaitu 18,75 cm per tanaman.Application Liquid Formulation Trichoderma harzianum and Trichoderma virens as Biological Control Agent (APH) Leaf Blight (Phytopthora palmivora) on Cocoa SeedlingsAbstract. Prevention of leaf blight by the fungus Phytophthora palmivora on cocoa (Theobroma cacao L.) using fungicides may adversely affect the environment and cause resistance, so to resolve it needed another alternative by using Biological Antagonist Agent (APH) such as Trichoderma spp. The multiplication of Trichoderma spp. can be reproduced with various media, one of them with a liquid formulation. This study aimed to determine the effect of the combination of T. harzianum and T.virens in controlling P. palmivora leaf blight in cocoa seedlings. This research was conducted at the Laboratory of Plant Disease and Experimental Garden of the Faculty of Agriculture, Syiah Kuala University in January 2018 until July 2018. This study used a non-factorial Completely Randomized Design consisting of 4 treatments with 5 replications. If the F test shows a real effect, it will continue by the smallest real difference test at the level of 5%. The variables observed were incubation period, disease incidence, disease severity, number of leaves, leaf width, stem diameter and plant height. The results of this study showed that the application of liquid formulation T. harzianum and T. virens had an effect on the incubation period, disease incidence, disease severity and plant height of 18.75 cm per plant.

Uji masa simpan pelet Trichoderma harzianum dan kemampuannya dalam menghambat perkembangan penyakit Layu Fusarium pada bibit tomat. Rizka Musfirah; Rina Sriwati; Tjut Chamzurni
Jurnal Ilmiah Mahasiswa Pertanian Vol 3, No 2 (2018): Mei 2018
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Abstrak. Tomat (Solanum lycopersicum) merupakan salah satu komoditas pertanian yang ditanam secara luas di seluruh dunia, termasuk di Indonesia, karena memiliki rasa yang khas dan enak, juga memiliki nilai gizi seperti sumber vitamin A dan C yang sangat baik. Produksi tomat mengalami penurunan setiap tahun, salah satunya diakibatkan oleh organisme penganggu tanaman (OPT) yaitu patogen Fusarium oxysporum sehingga perlu dilakukan pengendalian hayati yaitu menggunakan Trichoderma harzianum dalam bentuk formulasi pelet yang praktis, efektif, dan efesien. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 6 perlakuan dengan 3 ulangan, setiap perlakuan terdiri dari 10 unit bibit tomat. Penelitian ini terdiri dari 6 perlakuan yaitu perlakuan A (masa simpan pelet T. harzianum 4 minggu), B (masa simpan pelet T. harzianum 3 minggu), C (masa simpan pelet T. harzianum 2 minggu), D (masa simpan pelet T. harzianum 1 minggu), E (masa simpan pelet T. harzianum 0 minggu), F (tanpa perlakuan pelet T. harzianum). Peubah yang diamati yaitu pre-emergence damping off, post-emergence damping off, masa inkubasi, persentase tanaman layu, tinggi tanaman, dan jumlah daun. Hasil penelitian menunjukkan bahwa pelet T. harzianum yang disimpan 4 minggu efektif dalam menghambat perkembangan penyakit layu fusarium seperti menunda masa inkubasi sampai 7 HSI, menekan pre-emergence damping off sampai 90%, post-emergence damping off 92,95%, serta mampu meningkatkan tinggi tanaman sampai 19,63 cm dan meningkatkan jumlah daun rata-rata 7 helai pada 35 HSI. (Storing Period of Trichoderma harzianum Pellets and its ability to Inhibit the development of Fusarium Wilt Disease on Tomato Seeds)Abstract. Tomato (Solanum lycopersicum) is one of the most widely grown commodities in the world, including Indonesia. It has a distinctively good taste and many nutritional value such as vitamin A and C. However, tomato production has decreased every year. One of the main cause is the attacks by pathogens, named Fusarium oxysporum. A Biological control is necessary and the use of Trichoderma harzianum in the form of pellets is recommended because of its effectiveness, efficiency and practical use. This research used a Completely Randomized Design (RAL) non-factorial consisted of 6 treatments with 3 replications, each treatment consisted of 10 units of tomato seedlings. The 6 treatments are named as treatment A (T. harzianum pellet saving 4 weeks), B (T. harzianum pellet saving period 3 weeks), C (shelf life of 2 weeks T. harzianum pellet), D (shelf life of pellet T harzianum 1 week), E (shelf life of pellet T. harzianum 0 weeks), and F (without T. harzianum pellet treatment). The variables observed in this study are pre-emergence damping off, post-emergence damping off, incubation period, the percentage of wilted plants, plant height, and the number of leaves. The results showed that pellets of T. harzianum stored 4 weeks effectively inhibiting the development of fusarium wilt disease such as delaying incubation period up to 7 HSI (Days After Incubation), suppressing the pre-emergence damping off up to 90% and post-emergence damping off to 92.95%, also able to increase the plant height up to 19.63 cm and increase the average leaf number of 7 strands at 35 HSI.

APLIKASI KOMPOS TRICHODERMA DAN BIOCHAR DALAM MENINGKATKAN PRODUKSI PETANI BAWANG DI DESA DAYAH SUKON KABUPATEN PIDIE Nasrullah; Zulwanis; Said Mirza Pratama; Muhammad Resthu; Samadi; Rina Sriwati; Darusman; Kevin Rizqullah El-Ryiad
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i3.2382

Shallots are one of the leading commodities in Indonesia. The demand for shallots has increased, both for consumption and seeds, forcing Indonesia to import to meet these needs. Pidie Regency has been the center for shallot development in Aceh since 2019. This community service activity aimed to transfer knowledge and technology to farmers to produce compost and biochar independently in order to increase productivity and maintain the quality of land fertility. This activity is a demonstration plot for the implementation of the USK 2023 Kedaireka activities, in the fostered village of the Pionir Nusantara Foundation as a partner (Dayah Sukon Village, Pidie Regency) from August to November 2023. Mentoring activities were carried out through FGDs, providing knowledge about onion cultivation technology with sustainable microbial compost and biochar along with pest and disease management. Demonstration of good onion planting and production of Trichoderma and biochar compost and the application techniques were executed at the beginning of the activity. Proceeded with post-harvest and onion production results calculation based on farming efforts. Participants engaged in the activity enthusiastically, asking questions and telling stories about the cultivation practices they have done, learning how to make Trichoderma and biochar compost as well as the problems. Participants planted onion seeds using the land they have. Demonstration plots of sustainable onion cultivation practices with Trichoderma compost and Biochar in fostered villages increase production by 1:15, denoting that 1 kg of seeds produces 15 kg of onion bulbs. This effort is made to drive onions as primary commodity to support the community's economy and increase farmers' income, and be replicated by other farmers to invigorate Pidie Regency as the center for Aceh's shallots production.

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