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Alteration of Ecdysteroid Titre by Thyroxine and Juvenile Hormone Analogue (Methoprene) in Bombyx mori (Lepidoptera: Bombycidae) Ahmad, Intan; Rahayu, Resti; Permana, Agus D.; Astari, Sita
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.499 KB) | DOI: 10.24002/biota.v12i2.2666

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh hormon tiroksin dan analog hormon juvenil (metopren) terhadap pola titer hormon ekdisteroid hemolimf selama instar lima Bombyx mori. Instar dua B. mori diberi pakan daun murbei yang telah diberi tiroksin dengan konsentrasi 1500 ppm. Larva kemudian dibiarkan ganti kulit sampai instar tiga dan empat tanpa perlakuan. Tujuh puluh dua jam setelah ganti kulit pada instar lima, larva disuntik sebanyak 10 μl metopren dengan konsentrasi 15 atau 25 ppm. Titer ekdisteroid hemolimf dideteksi dengan Kromatografi Cair Kinerja Tinggi. Hasil yang diperoleh menunjukkan bahwa perlakuan dengan tiroksin ataupun kombinasi tiroksin-metopren menyebabkan terjadinya perubahan pola titer ekdisteroid dibandingkan dengan kontrol. Ekdisteroid terdeteksi dua hari lebih awal pada perlakuan tiroksin dan titer ekdisteroid pada puncak tertinggi lebih tinggi jika dibandingkan dengan kontrol. Kombinasi tiroksin-metopren tampaknya menyebabkan penekanan terhadap titer ekdisteroid. Hal ini terlihat dengan terjadinya penurunan titer ekdisteroid segera setelah penyuntikan dengan metopren sebelum mencapai titer puncak tertinggi.
Efisiensi Makan Spodoptera exigua (Lepidoptera: Noctuidae) pada Bawang Daun, Sawi Hijau dan Seledri di Laboratorium Hariani, Nova; Ahmad, Intan; Rahayu, Resti
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.523 KB) | DOI: 10.31258/jnat.14.1.86-89

Abstract

The aim of this research is to gain further understanding of how particular insect species respons to different host plants. This research isconsidered as preliminary research in integrated pest management for Spodoptera exigua. We report herein the consumption and utilizationof green onion (Allium fistulosum), caisin (Brassica rapa subsp. Parachinensis) and celery (Apium graveolens) by Spodoptera exigua. Theevaluation of indices of food consumption, growth and food utilization showed that caisin (Brassica rapa subsp. Parachinensis) was the bestfood for the last instar larvae of S. exigua as compared with celery or green onion. Growth rates and efficiency of conversion of ingestedfood to biomass (ECI) were significantly higher for larvae fed caisin (11.93%) than for those given green onion (8.06%) or celery (7.74%)Although the protein content of caisin (2.23%) was a bit lower than that of green onion (2.45%) but higher than celery (1.28%), we suspectthat the larvae performed best in caisin due to good balance between protein and carbohydrate as well as water content in caisin as comparedwith other host plants.
Alteration of Ecdysteroid Titre by Thyroxine and Juvenile Hormone Analogue (Methoprene) in Bombyx mori (Lepidoptera: Bombycidae) Intan Ahmad; Resti Rahayu; Agus D. Permana; Sita Astari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2666

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh hormon tiroksin dan analog hormon juvenil (metopren) terhadap pola titer hormon ekdisteroid hemolimf selama instar lima Bombyx mori. Instar dua B. mori diberi pakan daun murbei yang telah diberi tiroksin dengan konsentrasi 1500 ppm. Larva kemudian dibiarkan ganti kulit sampai instar tiga dan empat tanpa perlakuan. Tujuh puluh dua jam setelah ganti kulit pada instar lima, larva disuntik sebanyak 10 μl metopren dengan konsentrasi 15 atau 25 ppm. Titer ekdisteroid hemolimf dideteksi dengan Kromatografi Cair Kinerja Tinggi. Hasil yang diperoleh menunjukkan bahwa perlakuan dengan tiroksin ataupun kombinasi tiroksin-metopren menyebabkan terjadinya perubahan pola titer ekdisteroid dibandingkan dengan kontrol. Ekdisteroid terdeteksi dua hari lebih awal pada perlakuan tiroksin dan titer ekdisteroid pada puncak tertinggi lebih tinggi jika dibandingkan dengan kontrol. Kombinasi tiroksin-metopren tampaknya menyebabkan penekanan terhadap titer ekdisteroid. Hal ini terlihat dengan terjadinya penurunan titer ekdisteroid segera setelah penyuntikan dengan metopren sebelum mencapai titer puncak tertinggi.
Infeksi Subletal Bacillus thuringiensis pada Helicoverpa armigera: Mungkinkah Menyebabkan Resurgensi? Tjandra Anggraeni; Ramadhani Eka Putra; Intan Ahmad
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 1 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9946.571 KB) | DOI: 10.22146/jpti.10016

Abstract

Research on the effect of B. thuringiensis sublethal infection to the chance of H. armigera to resurgence has been conducted. Third instar insects were used and the concentration applied were 0 ppm, 150 ppm, 300 ppm, 450 ppm and 600 ppm. The result showed that along with the increase of B. thuringiensis infection, the number and the viability of the eggs production declined. In addition, the weight difference between the 3rd and the 4th instar became higher however between the 4th and the 5th became lower, moreover, the weight of female imago decreased, the life longevity of male and female imago were shorter and longer, respectively, along with the increase of B. thuringiensis infection. The possibility for resurgence will be discussed.
Dietary Compensatory Feeding in Manduca sexta (Lepidoptera: Sphingidae) Larvae Intan Ahmad
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 2 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.10071

Abstract

The aim of the experiments reported in this article is to gain further insight into the nature and control of dietary compensatory feeding: i.e., to investigate whether one or another nutrient is more important in eliciting compensatory behavior. The response of 4th-instar Manduca sexta to dilution of either protein or carbohydrate nutrient in the diet was measured. In these experiments, ratio of protein and carbohydrate selected, food consumption and utilization parameters were measured. Fourth instars were given the opportunity to self-select between two defined diets, one which differed between treatments in protein content and a complementary diet, which contained the standard amount of carbohydrate and did not vary from treatment to treatment. In response to protein dilution, the larvae compensated by consuming more of both the protein-containing and carbohydrate containing diets. In most of the cases, this compensation was sufficient to maintain normal growth, although AD, ECI, and ECD declined with the reduction of protein in the protein-containing diet. When larvae self-selected from a standard protein diet that was paired with a carbohydrate diet with one of six concentrations of sucrose, they responded by eating more from the protein-containing diet as the sucrose content of the carbohydrate diet declined. The growth and efficiency of food use was little affected over the entire range of carbohydrate diets tested.Although apparently the regulation of carbohydrate intake is not as strong as the regulation of protein intake, it seems that in response to dilution, the larvae select at each concentration a protein:carbohydrate ratio that is favorable to them.
Dosage Mortality Studies with Bacillus thuringiensis and Neem Extract on Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae) Intan Ahmad
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 2 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12750

Abstract

The objective of the study was to evaluate the responses of the diamondback moth, Plutella xylostella (L.) larvae to Bacillus thuringiensis and Neem extract. Therefore, dosage mortality studies using bioassay method were conducted on populations of P. xylostella from Garut, Pangalengan, and Lembang. Tests with B. thuringiensis, resulted in no significant differences in susceptibility between Garut and Pangalengan populations. However, those two populations were differed significantly in susceptibility to Lembang population which had the highest value of LC50 with resistance factor of 2.63, suggesting that a significant level of resistance against B. thuringiensis already occurred. In response to neem extract treatment, the results, as expected showed that there were no significant differences in susceptibility among the three populations. This indicates that neem extract could be used to control P. xylostella that has developed resistance to B. thuringiensis.
KORELASI FAKTOR CURAH HUJAN TRRHADAP DISTRIBUSI NYAMUK VEKTOR DEMAM BERDARAH AE. AEGYPTI DAN AE.ALBOPICTUS DI KOTA BANDUNG Rahmad Arya Fitra; Intan Ahmad
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 1 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i1.8802

Abstract

Kota Bandung adalah salah satu daerah yang dinyatakan DBD sebagai KLB dari tuhuh kota dan kabupaten. Penelitian ini bertujuan untuk mengetahui distribusi  vektor  nyamuk demam berdarah, Ae. aegypti  yang dikumpulkan dengan menggunakan metode survei ovitrap di lima Kecamatan endemis demam berdarah di Kota Bandung dan menganalisa hubungan faktor faktor curah hujan terhadap distribusi nyamuk Ae. aegypti di Kota Bandung. Penelitian ini dilakukan dengan menggunakan metode stratified random sampling .Sampel  berjumlah  400, disebar  di lima Kecamatan endemis di kota Bandung pada bulan September 2014 hingga Januari 2015. Hasil analisis korelasi pearson curah hujan berpengaruh signifikant terhadap perolehan rerata larva  Ae. aegypti (p: 0,025) p<0.05. Dari penelitian ini disimpulkan bahwa nyamuk  Ae. aegypti  sangat tinggi distribusinya dibanding dengan nyamuk Ae.albopictus  di Kecamatan endemis di Kota Bandung. Curah hujan berpengaruh terhadap distribusi nyamuk  Ae. aegypti di Kota Bandung Kata Kunci :  Dengue,Curah hujan, Aedes aegypti, Aedes Albopictus
Revitalisasi LPTK dalam Pengembangan Profesionalisme Tenaga Pendidik Menghadapi Era MEA Intan Ahmad
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Revitalisasi LPTK dalam Pengembangan Profesionalisme Tenaga Pendidik Menghadapi Era MEA
Status dan perkembangan resistensi Aedes aegypti (Linnaeus) (Diptera: Culicidae) strain Bandung, Bogor, Makassar, Palu, dan VCRU terhadap insektisida permetrin dengan seleksi lima generasi Yerslin Mantolu; Kustiati Kustiati; Trisnowati Budi Ambarningrum; Sri Yusmalinar; Intan Ahmad
Jurnal Entomologi Indonesia Vol 13 No 1 (2016): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.624 KB) | DOI: 10.5994/jei.13.1.1

Abstract

Aedes aegypti (Linnaeus) (Diptera: Culicidae) is the main vector of dengue fever. In Indonesia, insecticides, especially pyrethroids, such as permethrin, have been effectively used to control Ae. aegypti. Notwithstanding that permethrin has been used since 1980s, information regarding the status and development of resistance of Ae. aegypti to permethrin is still limited and need further update. This study was conducted using the WHO standard test method. The aims was to determine the resistance status, and changes in resistance level of four field strains (Palu, Makassar, Bandung, Bogor) and susceptible strain (VCRU) of Ae. aegypti after the selection with permethrin for five generations. The results showed that resistance status of all field strains to permethrin were considered as high. The value of RR50 ranged between 10.5 to 25.7 fold. Bandung strain had the highest value of  RR50 (22.5 fold), while Makassar strain had the lowest value of RR50 (10.5 fold). The fifth generation  (F5) of  five  selected  Ae. aegypti strains had the level of resistance 5 to 18 times higher than their parental. Knowledge of  resistance status in a given area accompanied with the understanding  about the development of resistance can be used to design a better vector management.
Aplikasi campuran serbuk kayu pinus dan fipronil sebagai umpan rayap tanah Macrotermes gilvus (Hagen) (Isoptera: Termitidae) di Bandung Amran Amran; Intan Ahmad; Ramadhani Eka Putra; Eko Kuswanto
Jurnal Entomologi Indonesia Vol 12 No 2 (2015): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.777 KB) | DOI: 10.5994/jei.12.2.73

Abstract

In this study, termite bait comprising pinewood of sawdust and a slow action insecticide, fipronil, was made and applied to control subterranean termite Macrotermes gilvus (Hagen) (Isoptera: Termitidae) in Bandung. Colony size was measured using capture-mark-release-recapture prior to the bait application, along with the measurement of wood consumption at each station.  Colony foraging populations at all stations were estimated to be 77,951 termites, with mean wood consumption rates ranging from 0.02 to 6.16 g/station/day. Observation at 18 stations installed with bait consisted of 40 ppm fipronil mixed with pinewood sawdust showed that number of foraging activity termites was effectively reduced in 40 days. It is concluded that fipronil-treated pinewood sawdust bait is effective in controlling the population of foraging M. gilvus workers in 40 days.