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All Journal Buletin Peternakan Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Pharmaceutical Science and Technology Biota Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology Pro-Life TREUBIA AGRIPEAT Jurnal Agritechno JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Jurnal Biogenerasi Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi JURNAL HUTAN TROPIS BIOTROPIC
Nirmala Fitria Firdhausi
Universitas Islam Negeri Sunan Ampel Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Optimization of PCR Protocol for the Identification of Yeasts Isolated from Apis mellifera Honeycomb based on the ITS rDNA Ayudya Fitri Arifa; Nirmala Fitria Firdhausi; Irul Hidayati; Yuanita Rachmawati; Moch. Irfan Hadi
Biota Vol 8 No 2 (2022): Jurnal Biota 2022
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v8i2.10181

Abstract

Yeast is a microorganism that can be found in honeycomb. Yeast identification is a process to find and identify new species. One of which is molecular identification of yeast with rDNA sequences in the ITS region. Before carrying out molecular identification, it is necessary to optimize yeast DNA amplification methods to obtain good DNA sequences that ease the yeast identification process. The purpose of this study was to discover the optimum PCR (Polymerase Chain Reaction)protocols for the identification of yeasts isolated from Apis mellifera honeycomb based on the ITS rDNA. This study used 3 PCR (Polymerase Chain Reaction) protocols, i.e., from Kanti et al. (2018), Ediningsari (2008), and Maulana (2011). This results study shows that the optimum PCR protocol was from Maulana (2011), which produced clear and whole DNA fragment luminescences.
Phylogenetic Study of Madura Cattle Based on Mitochondrial Cyt b and D-loop Sequences Nirmala Fitria Firdhausi; Achmad Farajallah; Dyah Perwitasari
Buletin Peternakan Vol 45, No 1 (2021): BULETIN PETERNAKAN VOL. 45 (1) FEBRUARY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i1.48557

Abstract

Madura Cattle is one breed of local cattle from Indonesia. Madura cattle are estimated to originate from a crossbreeding between Bos indicus and Bos javanicus. Another presumption is that Madura cattle are the result of a crossbreeding between B. indicus males and mixed B. javanicus or Bos taurus. Tracing the history of Madura cross and another cattle phylogenetic based on maternal lineage can be done by analyzing the variation of the mitochondrial genome (mtDNA). The purpose of this study was to determine the clarity of the origin of Madura cattle based on maternal lineage using mtDNA markers Cyt b and D-loop. This research is expected to provide genetic information and the origin of Madura cattle, so that it can be used to help improve the breeding and conservation program for Madura cattle. The results of the phylogeny tree reconstruction, using the Cyt b and D-loop genes showed that Madura cattle originated from Sampang region (Polagan, Golbung, and Komis) were grouped into two types of maternal origin. Madura cattle clade I are grouped with B. indicus and B. taurus, while Madura cattle clade II are grouped with B. javanicus. A crossbreeding between B. javanicus and B. indicus is estimated to have been carried out since the entry of Hindu culture brought by the India peoples to Indonesia around 1800 years ago. The crossing between B. javanicus and B. indicus was then more intensively carried out at the time of the government's promoting the development of Ongol cattles (B. indicus) in the days of the Dutch East Indies. The length segment of Cyt b that can be amplified is 230 bp and the D-loop segment of varying length, 577 bp for the Madura 41 and 29 samples, and 624 bp for sample 32.  
Pengenalan Makroinvertebrata Bentik sebagai Bioindikator Pencemaran Perairan Sungai pada Siswa di Wonosalam, Mojokerto, Jawa Timur Nirmala Fitria Firdhausi
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 5 No. 3 (2019): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.5.3.210-215

Abstract

The river in Wonosalam is upstream of the rivers that flow in the Mojokerto and Jombang regions. As an upstream area, monitoring water quality of the river is needed so that pollution can be detected early. Water quality monitoring activities can be carried out using benthic macroinvertebrates indicator. The purpose of this PKM activity was to introduce water quality monitoring methods used benthic macroinvertebrates as bioindicators for students in the Wonosalam sub-district area. The main target of this activity is students of SLTPN 1 Wonosalam. The method used was lecturing, direct practicing, and discussion. Introduction of the benthic macroinvertebrates as a bioindicator was carried out very well: the students were very enthusiastic in the implementation activities from beginning to end, the students were quite active in the sampling process until the identification process, there was an increase in the students knowledge about bioindicators and the students were able to distinguish groups, EPT (Ephemeroptera, Plecoptera, and Trichoptera) and non-EPT. Based on benthic macroinvertebrate sampling the result showed that the value of the Sumber Bengawan river are 6.25, indicated that Sumber Bengawan river was not polluted.
FIRST REPORT ON THE FEEDING BEHAVIOR OF EARLESS MONITOR, LANTHANOTUS BORNEENSIS AND ITS PREDATION ON RICE FIELD FROG, FEJERVARYA LIMNOCHARIS IN A CAPTIVE ENVIRONMENT Ahmad Nauval Arroyyan; Evy Arida; Nirmala Fitria Firdhausi
TREUBIA Vol 48, No 2 (2021)
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/treubia.v48i2.4115

Abstract

Being endemic to Borneo, the Earless monitor, Lanthanotus borneensis (Steindachner, 1878) is rarely found in its habitats due to its cryptic behavior. We provide care for confiscated animals in the Reptile House of Museum Zoologicum Bogoriense (MZB) in Cibinong, West Java, Indonesia since 2014. Little is known on its natural prey but from scattered descriptive reports. This study is aimed at documenting the feeding behavior of ten captive Earless monitors and events of predation on frogs. We set up two experiments, one with meat of Rice Field Frog, Fejervarya limnocharis, and the other with live frog of the same species. Our recorded observations ran for four weeks for the frog meat feeding experiment and followed by the frog predation experiment. Our results showed that lizards constantly accepted frog meat. Lizards tended to feed before sunset for a short period of time on the muddy soil surface, although a few individuals inconsistently fed under water. The average body mass for these lizards increased by 4.29 g and average SVL by 0.45 cm. We recorded predation on frogs in three out of ten individuals observed during this study. If F. limnocharis is confirmed to occur in the natural habitats of L. borneensis, it is possible that this frog species is among the natural prey for Earless monitors. Further studies on its natural diets should be conducted to gain in-depth knowledge essential for generating effective captive husbandry for this nationally protected species in Indonesia.
Respon Tanaman Daun Tombak (Sagittaria lancifolia) Dalam Cekaman Logam Berat Tembaga (Cu) Alfin Fatwa Mei Afifudin; Eva Agustina; Nirmala Fitria Firdhausi; Rony Irawanto
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 7, No 2 (2022): Mei 2022
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v7i2.1118

Abstract

One type of heavy metal that pollutes waters is heavy metal copper (Cu). Cu metal is basically an essential metal for living things, but with high levels of Cu metal, it can interfere with the body's metabolic processes. Therefore, there needs to be a solution to overcome Cu metal pollution in the waters. One of them is phytoremediation, or the use of plants to absorb pollutants. Spear leaf plant (Sagittaria lancifolia) is proven to be able to absorb heavy metal copper (Cu). However, the growth response is not yet known in more detail. Therefore, this study aimed to determine the growth response of pike leaf (Sagitaria lancifolia) under stress of heavy metal copper (Cu). This research is an experimental study with a completely randomized design (CRD). The experiment was conducted with 8 treatments (4 treatments with 2 tests) and 3 replications. The 4 treatments were distinguished based on differences in the concentration of Cu metal used, namely 0 mg/L, 1mg/L, 2mg/L, and 3 mg/L. The results showed that exposure to heavy metal copper (Cu) in each concentration variation and detention time variation did not have a significant effect on the growth and morphological conditions of spear leaf (Sagittaria lancifolia) plant, so this plant deserves to be used as an option in recovery efforts of water environment contaminated with heavy metal copper (Cu).Keywords – Response, Lanceleaf arrowhead, Sagittaria lancifolia, Heavy metals, Copper (Cu)
Potensi Limbah Sisik Ikan Sebagai Kitosan dalam Pembuatan Bioplastik Ajeng Ayu Ramadhani; Nirmala Fitria Firdhausi
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 6, No 2 (2021)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v6i2.782

Abstract

Bioplastics are environmentally friendly plastics derived from natural materials. Bioplastics are easier to decompose when compared to commercial plastics. Bioplastics are generally made from starch contained in plants. But the use of starch as a base material has the disadvantage of producing bioplastics that are not waterproof. Therefore, it is necessary to add chitosan to improve bioplastic characteristics. Chitosan usually comes from the shell of crustacean animals, but it turns out that in fish scales waste, also contains chitosan. Fish scales are a by-product of the process of fish processing. Fish scales are only discarded and not utilized so that they become waste that can pollute the environment. Fish scales have a chitin content that can process into chitosan, which can be useful as an additional ingredient in the manufacture of bioplastics. This review aims to find out the potential of fish scales waste as chitosan in the manufacture of bioplastics. Based on the results of previous research, fish scales have a chitosan content that can use as an additional ingredient in the manufacture of bioplastics. Fish scale chitosan can form bioplastic film with characteristic brownish-yellow film. The addition of fish scale chitosan is also able to improve the water resistance of bioplastic film. Based on the results of the review can be concluded bring fish scales to have the potential as chitosan that can use in the manufacture of bioplastic.Keywords – Bioplastic, Chitosan, Fish Scales. 
Struktur Komunitas Collembola Pada Habitat Gua Lowo dan Kebun Warga di Desa Melirang Kabupaten Gresik, Jawa Timur Ria Safitri; Siti Zulaikha; Saiful Bahri; Saiku Rokhim; Ita Ainun Jariyah; Nirmala Fitria Firdhausi
Jurnal Pro-Life Vol. 9 No. 3 (2022): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Collembola is one of the soil fauna that acts as the smallest constituent component for the balance of the ecosystem. This study aims to compare the structure of the Collembola community in the habitat of Lowo Cave and Citizen's Garden in Melirang Village, Gresik Regency. This research uses descriptive exploratory research with soil drill method for soil sampling and pitfall trap for ground surface insect traps. Based on Collembola data obtained in Lowo Cave, there are 3 species with a total of 87 individuals, while in Citizen's Garden there are 6 Collembola species with a total of 145 individuals The results of the analysis of this study indicate that the Diversity Index value in the Lowo Cave habitat is H'= 0.859735 and in the Citizen's Garden a habitat with the value H' = 1.475675, the Evenness Index value in the Lowo Cave is E = 0.782565 and in the Citizen's Garden with a value of E = 0.82359, the value of the Dominance Index in Gua Lowo is 0.48104 and in the Citizen's Garden is 0.282949, the Relative Frequency of Ascocyrtus sp. and Hypogastrura consanguinea have a relative frequency of 100%.
Diversity and Community Structure of Dragonflies (Odonata) in Various Types of Habitat at Lakarsantri District, Surabaya, Indonesia Muhamad Azmi Dwi Susanto; Nirmala Fitria Firdhausi; Saiful Bahri
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76690

Abstract

Dragonflies are insects that are very dependent on the existence of freshwater ecosystems, However, the population of dragonflies in urban freshwater ecosystems is at risk due to a number of issues. Consequently, it is essential to carry out research and efforts to preserve dragonflies in urban areas. This study aims to provide information about the diversity and structure of dragonfly communities in various habitat types in the Lakarsantri, Surabaya. Data collection in this study was carried out in the habitat types of ponds, reservoir, river, and rice field in July to September 2021. The study results show there are 22 species from 4 families with a total of 827 individuals. Analysis of the Shannon-Wiener diversity index showed that the highest value of dragonfly diversity was found in a pond, with a value of H' = 2.40, and the location with the lowest value was a river, with a value of H' = 1.77. At four research locations that have different aquatic ecosystems, the community structure of dragonfly is also different. The composition of the dragonfly community structure at the reservoir location has similarities to a pond, and at a river location, it has similarities to a paddy field. Differences in abiotic factors consisting of light intensity, humidity, and temperature at each study location have a correlation with differences in dragonfly community structure. In addition, the composition of the vegetation at each location is also one of the factors causing differences in the structure of the dragonfly community. 
PENGARUH KONSENTRASI DAN LAMA PERENDAMAN KOLKISIN TERHADAP KARAKTER POLIPLOIDI PADA TANAMAN LILI HUJAN (Zephyranthes rosea Lindl.) Putri, Vivin Diana; Faizah, Hanik; Firdhausi, Nirmala Fitria
Jurnal Pro-Life Vol. 10 No. 3 (2023): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Zephyranthes rosea Lindl. is one of Indonesia's export commodities. Research on the induction of polyploid Z. rosea to increase sales value by increasing its phenotype is still limited and limited to seed organs. The aim of this research was to determine the effect of colchicine concentration, soaking time, and the interaction of the two on polyploidy characters. Z. rosea root organs were treated with colchicine (0%, 0.025%, 0.05%, 0.075%, and 0.1%) and different soaking time (6 hours, 12 hours, 18 hours, and 24 hours) in this study. The result showed that there was a significant effect of colchicine concentration, soaking time, and the interaction of of the two on leaf length, stomata density, and dry weight while flowering time, leaf width, stomata length, stomata width, fresh weight, and flower color were not significantly affected. The lowest leaf length was produced by 0.1% colchicine (21.392 cm), 6 hours of soaking time (22.693 cm), and the interaction of 0.1% colchicine with 6 hours of soaking time (20.633 cm). The highest density of stomata resulted from 0.025% colchicine (787.247/mm2), 6 hours of soaking time (762.626/mm2), and the interaction of 0.025% colchicine with 24 hours of soaking time (906.566/mm2). The highest dry weight resulted from colchicine 0.075% (16.433 gram), soaking time of 12 hours (16.527 gram), and interaction of colchicine 0.075% with soaking time of 12 hours (19.467 gram). Because there were more polyploid characters which were not significantly affected, the polyploid Z. rosea had not been formed in this study.
EFEKTIVAS BEAUVERIA BASSIANA TERHADAP PERTUMBUHAN TANAMAN TOMAT (Solanum lycopersicum L.) YANG TERINFEKSI CENDAWAN Fusarium oxysporum: EFFECTIVENESS OF BEAUVERIA BASSIANA ON THE GROWTH OF TOMATO PLANTS (Solanum Lycopersicum L.) INFECTED WITH THE FUNGUS Fusarium Oxysporum Khodijah, Siti; Firdhausi, Nirmala Fitria; Faizah, Hanik
AgriPeat Vol. 25 No. 02 (2024): JURNAL AGRIPEAT VULUME 25 NOMOR 02 SEPTEMBER 2024
Publisher : Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/agp.v25i02.15185

Abstract

Tanaman tomat (Solanum lycopersicum L.) banyak dibudidayakan hampir diseluruh dunia. Tiap tahun tanaman tomat memiliki penurunan produksi yang diakibatkan oleh banyak hal, salah satunya adalah serangan Fusarium oxysporum. Salah satu cara untuk mengendalikan penyakit F. oxysporum adalah dengan penggunaan agen pengendali hayati, yaitu Beauveria bassiana. Selain dapat meningkatkan ketahanan tanaman dari serangan organisme pengganggu, B. bassiana juga memacu pertumbuhan dan perkembangan tanaman. Penelitian ini bertujuan untuk menentukan waktu pemberian B. bassiana yang efektif dalam mengatasi serangan layu tanaman tomat. Desain penelitian ini menggunakan RAL. Pengujian B. bassiana dilakukan pada 3 dan 7 hari sebelum tanam, 3 dan 7 hari sesudah tanam, dan saat tanam. Data diuji menggunakan ANOVA dan Duncan 5%. Hasil menunjukkan B. bassiana telah memenuhi standar mutu sebagai agen pengendali hayati sesuai Keputusan Menteri Pertanian No. 70/Permentan/SR.140/19/2011 serta telah sesuai SNI 8027.1: 2014. Seluruh perlakuan pemberian B. bassiana mampu menekan kelayuan patogen hingga 0,4 %, B. bassiana juga berpengaruh pada tinggi tanaman, jumlah cabang, jumlah daun, panjang akar, panjang daun, bobot kering dan basah tanaman. Pada penelitian ini perlakuan B. bassiana terbaik untuk pertumbuhan dan hasil adalah T4, yaitu pemberian B. bassiana 3 hari setelah tanam.
Co-Authors ACHMAD FARAJALLAH AGUSTINA, EVA Ahmad Nauval Arroyyan Ajeng Ayu Ramadhani Alfin Fatwa Mei Afifudin Arifin, Siti Hakimah Aprilia Garini Atiqoh Zummah Ayudya Fitri Arifa Choiriyah, S.Si, Chumaidatul DYAH PERWITASARI -FARAJALLAH Esti Tyastirin Evy Arida Faizah, Hanik Farah Syifa Zayyanna, Salma Fatiha, Irssa Intan Febiyanti, Salma Fakhirah Hanik Faizah Intan Ahmad Intan Ahmad Irul Hidayati Ita Ainun Jariyah Mabruroh, Arofatul Syabina Matlubah, sheva Misbakhul Munir Moch. Irfan Hadi Muhamad Azmi Dwi Susanto Muhtar, Nurul Maulidiya Putri, Vivin Diana RIA SAFITRI Rony Irawanto Saiful Bahri Saiful Bahri Saiku Rokhim Sarmawaty Kotala Siti Khodijah Siti Zulaikha Wati, Diana Rahma Wibisana, Oki Rahmatirta widya pertiwi Yuanita Rachmawati Zakki Mubarak