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All Journal Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Journal of Dentomaxillofacial Science MAHESA : Malahayati Health Student Journal Jurnal of Syiah Kuala Dentistry Society
Syaflida, Rahmi
Departement Of Oral And Maxillofacial Surgery, Faculty Of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Journal : Jurnal Kedokteran Gigi Universitas Padjadjaran

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Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-DawleyEffectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats Hendry Rusdy; Astri Suryani Pasaribu Saruksuk; Rahmi Syaflida Dalimunte; Gostry Aldica Dohude
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 2 (2021): Agustus 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i2.32563

Abstract

Pendahuluan: Pencabutan gigi merupakan prosedur yang sering dilakukan di kedokteran gigi. Setelah pencabutan gigi akan dihasilkan suatu perlukaan. Proses penyembuhan luka dapat dipercepat pada kondisi tertentu. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah getah tanaman betadine (Jatropha multifida L.). Penelitian bertujuan untuk menganalisis efektivitas getah tanaman betadine terhadap penyembuhan luka dan terhadap tanda-tanda infeksi pasca pencabutan gigi Metode: Desain penelitian studi eksperimental laboratorium dengan rancangan penelitian post-test only control group design menggunakan 30 ekor tikus Sprague-Dawley. Teknik pengambilan sampel yaitu convenience sampling. Sampel dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan diberikan getah tanaman betadine dan kelompok kontrol diberikan asam traneksamat secara oral menggunakan sonde lambung. Tunggu selama 4 jam setelah pemberian getah tanaman betadine dan asam traneksamat. Anestesi pada tikus menggunakan ketamin 50 mg/kg berat badan secara intramuskular kemudian dilakukan pencabutan pada gigi tikus. Pengamatan dilakukan dengan melihat kriteria klinis pada hari 1,3,7 dan diperhatikan sampai luka sembuh serta lihat tanda-tanda infeksi. Analisis data dilakukan dengan uji normalitasShapiro Wilik. Hasil penelitian menunjukkan bahwa data berdistribusi tidak normal. Analisis data dilanjutkan menggunakan uji statistik mann whitney. Hasil: Terdapat perbedaan signifikan penyembuhan luka soket pasca pencabutan gigi setelah diberikan getah betadine dan asam traneksamat dengan nilai p=0,037 (p<0,005). Simpulan: Pemberian getah tanaman betadine terbukti lebih efektif terhadap proses penyembuhan luka soket pasca pencabutan gigi dibandingkan dengan pemberian asam traneksamat. Kata kunci: tikus Sprague-Dawley; penyembuhan luka; pencabutan gigi; getah batang betadine ABSTRACTIntroduction: Tooth extraction is a procedure often performed in dentistry. Tooth extraction will always cause injuries. However, the wound healing process can be accelerated under certain conditions. One of the natural ingredients that can accelerate the wound healing process is betadine (Jatropha multifida L.) plant sap. The study was aimed to analyzed the effect of betadine plant sap on wound healing and signs of infection after tooth extraction. Methods: Experimental laboratory study design with post-test only control group design was conducted towards 30 Sprague-Dawley rats. The sampling technique was convenience sampling. The sample was divided into two groups, the treatment group and the control group. The treatment group was administered with betadine plant sap, and the control group was administered with tranexamic acid orally using a gastric probe, then waited 4 hours after. The anaesthesia was then performed using 50 mg/kg body weight of ketamine intramuscularly. The extraction was performed after. Observations was conducted at the clinical criteria on days 1, 3, and 7 and continue to be monitored until the wound heals. Then, the signs of infection were observed. Data analysis was carried out using the Shapiro Wilk normality test. The results showed that the data was not normally distributed. Thus, data analysis was continued using the Mann Whitney statistical test. Results: The results showed a significant difference in the healing of socket wounds after tooth extraction after being administered with betadine sap and tranexamic acid with a value of p=0.037 (p<0.005). Conclusions: Administration of betadine plant sap is proven to be more effective in accelerating the healing process of socket wounds after tooth extraction than tranexamic acid. Keywords: Sprague-Dawley rats; wound healing; pencabutan gigi; getah batang betadine 
Pengaruh ekstrak daun kemangi (Ocimum basilicum L) terhadap Staphylococcus aureus di rongga mulutEffect of basil leaf extract (Ocimum basilicum L.) on oral Staphylococcus aureus Putri Widya Utami; Isnandar Isnandar; Rahmi Syaflida; Indra Basar Siregar
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 1 (2021): April 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i1.29968

Abstract

Pendahuluan: Staphylococcus aureus merupakan mikroorganisme dalam rongga mulut yang bersifat patogen. Di Indonesia, penyakit infeksi masih menjadi masalah umum terutama pada rongga mulut, untuk itu dikembangkan obat antibakteri yang berasal dari tumbuhan salah satunya daun kemangi. Tujuan penelitian untuk menganalisis efektivitas penggunaan ekstrak daun kemangi (Ocimum basilicum L) terhadap jumlah Staphylococcus aureus rongga mulut. Metode: Penelitian ini menggunakan true eksperiment laboratoris, dimana pengujian efektivitas antibakteri dengan metode pengenceran seri. Sampel yang digunakan strain murni Staphylococcus aureus dan isolat klinik Staphylococcus aureus. Konsentrasi ekstrak yang digunakan 50%, 25%, 12,5%, 6,25% dan dilakukan pengulangan sebanyak 4 kali. Data penelitian diolah menggunakan SPSS yaitu uji Kruskal-Wallis dan uji Mann-Whitney.Hasil: Konsentrasi 50% merupakan kadar bunuh minimum (KBM) untuk strain murni bakteri Staphylococcus aureus, dan kadar hambat minimum (KHM) untuk isolat klinik Staphylococcus aureus. Konsentrasi 25% hanya didapati kadar hambat minimum (KHM) untuk strain murni Staphylococcus aureus. Simpulan: Terdapat pengaruh penggunaan ekstrak daun kemangi (Ocimum basilicum L) terhadap penurunan jumlah Staphylococcus aureus rongga mulut.Kata kunci : Daun kemangi, Staphylococcus aureus, pengenceran seri, KHM, KBM. ABSTRACTIntroduction: Staphylococcus aureus is a pathogenic microorganism in the oral cavity. In Indonesia, infectious diseases are still a common problem, especially in the oral cavity. Therefore, a natural antibacterial remedy has been developed, one of which is basil leaves. The study aimed to analyse the effectiveness of using basil leaf extract (Ocimum basilicum L) against the number of oral Staphylococcus aureus. Methods: This study used a true laboratory experiment, where the antibacterial effectiveness was tested by using the series dilution method. The samples used were pure strains of Staphylococcus aureus and clinical isolates of Staphylococcus aureus. The extract concentration used was 50%, 25%, 12.5%, 6.25% and was repeated 4 times. The research data were processed using SPSS, namely the Kruskal-Wallis test and the Mann-Whitney test. Results: The concentration of 50% is the minimum kill rate (MKC) for pure strains of Staphylococcus aureus and the minimum inhibitory level (MIC) for clinical isolates of Staphylococcus aureus. At 25% concentration, only the minimum inhibitory level (MIC) was found for pure strains of Staphylococcus aureus. Conclusion: There is an effect of using basil leaf extract (Ocimum basilicum L) on reducing the number of oral Staphylococcus aureus.Keywords: Basil leaf, Staphylococcus aureus, serial dilution, MIC, MKC.
Uji efektivitas ekstrak kulit jengkol (Pithecellobium lobatum benth.) terhadap pertumbuhan Streptococcus viridans: studi eksperimental laboratoris Syaflida, Rahmi; Ramayani, Al Shella; Isnandar, Isnandar; Riza, Ahyar
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.57875

Abstract

Pendahuluan: Kulit jengkol (Pithecellobium lobatum Benth.) merupakan limbah organik yang melimpah di pasar tradisional namun belum dimanfaatkan secara optimal. Kulit jengkol mengandung senyawa aktif seperti alkaloid, flavonoid, glikosida, steroid antrakinon, tanin, saponin, dan asam fenolat yang diketahui memiliki potensi antibakteri. Senyawa-senyawa ini dapat menghambat pertumbuhan bakteri dengan merusak struktur peptidoglikan pada dinding sel sehingga integritas sel terganggu. Penelitian ini bertujuan untuk menganalisis aktivitas antibakteri ekstrak kulit jengkol terhadap Streptococcus viridans secara in vitro. Metode: Penelitian eksperimental ini menggunakan rancangan post-test only control group design. Ekstraksi kulit jengkol dilakukan dengan metode maserasi, kemudian dilarutkan dalam DMSO menjadi lima konsentrasi (5%, 10%, 20%, 40%, 80%). Chlorhexidine gluconate 0,2% digunakan sebagai kontrol positif, sedangkan DMSO sebagai kontrol negatif. Uji antibakteri dilakukan dengan metode difusi cakram Kirby-Bauer pada media MHA dengan empat kali pengulangan. Analisis data dilakukan menggunakan SPSS versi 29 dengan uji normalitas Shapiro-Wilk, homogenitas Levene test, dilanjutkan ANOVA dan Post Hoc LSD. Hasil: Rata-rata diameter zona hambat meningkat seiring konsentrasi: 5% (7,325 mm), 10% (9,550 mm), 20% (10,400 mm), 40% (11,800 mm), dan 80% (14,775 mm). Terdapat perbedaan signifikan antar kelompok (p=0,001), kecuali antara konsentrasi 10%-20%, 20%-40%, dan 80% dengan kontrol positif (p>0,05). Simpulan: Ekstrak kulit jengkol efektif menghambat pertumbuhan Streptococcus viridans secara in vitro.Effectiveness test of jengkol peel extract (Pithecellobium lobatum benth.) against Streptococcus viridans growth: a laboratory experimental studyIntroduction: Jengkol peel (Pithecellobium lobatum Benth.) is an abundant organic waste often discarded in traditional markets but remains underutilized. It contains active compounds such as alkaloids, flavonoids, glycosides, steroids, anthraquinones, tannins, saponins, and phenolic acids, many of which exhibit antibacterial potential. These compounds can inhibit bacterial growth by disrupting the peptidoglycan structure in the bacterial cell wall, thereby compromising cell integrity. This study aimed to analyze the antibacterial effectiveness of jengkol peel extract against Streptococcus viridans in vitro. Methods: This laboratory experimental study used a post-test-only control group design. Jengkol peel was extracted using the maceration method and dissolved in DMSO to obtain five concentrations (5%, 10%, 20%, 40%, and 80%). Chlorhexidine gluconate (0.2%) served as a positive control, and DMSO as a negative control. The antibacterial activity was evaluated using the Kirby-Bauer disc diffusion method on Mueller-Hinton agar (MHA), with four replicates per treatment. Data were analyzed using SPSS version 29 with Shapiro-Wilk test for normality, Levene’s test for homogeneity, followed by one-way ANOVA and post hoc LSD tests. Results: The mean inhibition zone diameters increased with higher concentrations: 5% (7.325 mm), 10% (9.550 mm), 20% (10.400 mm), 40% (11.800 mm), and 80% (14.775 mm). Significant differences were observed among treatment groups (p=0.001), except between 10%-20%, 20%-40%, or 80% and the positive control (p>0.05). Conclusion: Jengkol peel extract demonstrates antibacterial activity against Streptococcus viridans in vitro.
Co-Authors Abdullah Oes Abdullah Oes Ahyar Riza Ahyar Riza Ahyar Riza Ahyar Riza Arisya Fiqriyah Astri Suryani Pasaribu Saruksuk Ayu R. Nabila Diana Sofia Hanafiah Dohude, Gostry Aldica Hasibuan, Sukri Paramita Hendry Rusdy Hendry Rusdy Indra B. Siregar Indra B. Siregar Indra Basar Siregar Isnandar Isnandar Jasmine Jasmine Jeanie T. Tanslie Jeanie T. Tanslie Marshall Sitorus Marshall Sitorus Muhammad R. Fauzie Olivia A. Hanafiah Olivia Avriyanti Hanafiah Putri Widya Utami Ramayani, Al Shella Ratnaningtyas, Jemima Ravichandran, Pavitra Riza, Ahyar Rusdy, Hendry Sepriyani, Risa Siregar, Indra Basar Yuni Nurul Fairuz