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Origin, Stemness, Marker and Signaling Pathway of Oral Cancer Stem Cell Dicha Yuliadewi Rahmawati; Hernindya Dwifulqi; Ferry Sandra
Molecular and Cellular Biomedical Sciences Vol 4, No 3 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i3.159

Cancer constitutes of complex heterogeneous organ-like structures with a hierarchical cell structure, and only minor phenotypic subpopulations with stem-like properties have a dual capacity to indefinitely self-renew and generate all heterogeneous cell phenotypes consisting of bulk tumor cells. Cancer stem cells (CSC) has similar properties to ordinary stem cells. It is understood that CSC is responsible for the recurrence of metastasis and drug resistance. Thus, control of CSC can provide successful therapy intervention that inhibits cancer growth and aggressive behavior. Conventional cancer therapy is realized to be insufficient for oral cancer therapy. Meanwhile, accurate targeting of OCSC has proved to be a significant challenge due to the commonality of many markers between OCSC and healthy cells. This article discusses the current understanding of oral CSC, with focus on origin, stemness, marker and signalling pathway.Keywords: oral cancer stem cell, CSC, marker, origin, stemness, therapy

Effect of hexane extract of Clinacanthus nutans leaves on HSC-3 cells migration in vitro Roeslan, Moehamad Orliando; Qurrotuain, Noni Anis; Rahmawati, Dicha Yuliadewi; Monthanpisut, Paopanga Orliando
Majalah Kedokteran Gigi Indonesia Vol 9, No 3 (2023): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.89452

Cancer is a major health issue and considered the most life-threatening disease worldwide. The oral cavity is one of the top ten locations in the body most frequently affected by cancer. The most common type of oral cancer (90%) is squamous cell carcinoma. Leaves of C. nutans have many uses and benefits, one of which is its potential as an anticancer agent. This study aims to determine the effect of C. nutans leaves hexane extract on HSC-3 cell strain migration. The leaves of C. nutans were extracted using a hexane solvent with the maceration method. Various concentrations (500, 250, 100, 50 and 25 mg/mL) were assessed to identify the effect of C. nutans leaves hexane extract on HSC-3 cells migration at 0, 6, 12, 24 and 48 time point. The concentration of 500 μg/mL was found to possess the ability to inhibit migration and induce apoptosis by the 6th hour. The concentration of 250 μg/mL induced apoptosis by the 24th hour. Concentrations of 100 μg/mL and 50 μg/mL were capable of inhibiting cell migration, and the concentration of 25 μg/mL could only delay cell migration up to the 12th hour. In conclusion, hexane extract of C. nutans leaves can inhibit HSC-3 cells migration.

Antibacterial effects of tomato ethanol extract (Solanum lycopersicum L.) against S. mutans and P. gingivalis: a laboratory experiment Rahmawati, Dicha Yuliadewi; Kurniawan, Viola Febi; Sanjaya, Oei Tirza Cintya; Sugiaman, Vinna Kurniawati; Mandalas, Henry Yonatan
Padjadjaran Journal of Dentistry Vol 35, No 3 (2023): November 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no3.50582

ABSTRACT Introduction: Process of dental plaque formation consists of three stages, pellicle formation, initial colonization facultative gram-positive one of microbes is S. mutans, secondary colonization and microbial maturation by Porphyromonas intermedia, P. gingivalis, Fusobacterium nucleatum. Formation of dental biofilm is considered to be main etiology of chronic periodontitis. Bacteria that often involved in chronic periodontitis are P. gingivalis. Tomatoes (Solanum lycopersicum L.) content lycopene compounds, flavonoids, and saponins have been shown to inhibit or kill bacterial growth. The aim is to analyze the antibacterial effect of servo tomato ethanol extract against S. mutans and P. gingivalis. Methods: This research was a laboratory experimental one with a posttest only control group design where observed variable diameter of the inhibition zone was produced from ethanolic extract of tomato (Solanum lycopersicum L.) in various concentrations of 3.125, 6.25, 12.5, 25, 50 and 100% chlorhexidine 0.2% as a positive control, negative control of distilled water against S. mutans and P. gingivalis on blood agar media. The method used in this study was the well-diffusion test. Results: The highest inhibitory zone at 100% concentration with a diameter of 32.10 mm was very strong; lowest inhibition at 3.123% was 3.95 mm, weak classification and against P. gingivalis at 3.125%, it was 3.72 mm, weak classification, meanwhile at 100% concentration, it was 9.67 mm, medium classification. The results of the One Way ANOVA statistical test showed a p<0.05 which had a significant effect in inhibiting the growth of S. mutans and P. gingivalis. Conclusions: There was an antibacterial effect of the ethanolic extract of tomato against S.mutans produced a larger diameter and stronger inhibition zone meanwhile against P.gingivalis produce medium inhibition zone. The content of tomatoes such as lycopene compounds, flavonoids, and saponins have been shown to inhibit or kill bacterial growth.Keyword : tomato ethanol extract (Solanum lycopersicum L.), S. mutans, P. gingivalis, antibacterial

The influence of chlorhexidine gluconate and povidone iodine on the minimum inhibitory cencentration and quantitative of streptococcus mutans Apriani, Anie; Mandalas, Henry Yonatan; Kurnia, Calvin; Rahmawati, Dicha Yuliadewi; Lison, Thio; Mulyadi, Nabila
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.%p

Background: An imbalance in the normal flora of the oral cavity can cause dental caries. The prevalence of caries in primary teeth in Indonesia which experiences caries is 90.2%, which shows that the prevalence of caries is still quite high. One way to prevent bacterial activity is using mouthwash. Chlorhexidine gluconate and Povidone Iodine are antibacterial agents that are effective in inhibiting bacterial growth, through bacteriostatic and bactericidal actions. This study aims to determine whether there is an inhibitory effect of chlorhexidine gluconate and povidone iodine on the growth of Streptococcus mutans (quantity).Method: This study used the broth microdilution method by treating Streptococcus mutans using 0.1 % chlorhexidine gluconate and 1% povidone iodine. The effect of inhibitory power was measured using the broth microdilution technique and the total plate count technique in the form of Colony Forming Units (CFU). Statistical calculations use the Shapiro-Wilk normality test, Levene's homogeneity test and Tukey's Post Hoc test to determine differences between treatments.Result: The results of the research showed that the treatment group in the form of 0.1% chlorhexidine gluconate and 1% povidone iodine had a very significant effect on the growth of bacterial colonies, which was very significant for the growth of Streptococcus mutans bacterial colonies.Conclusion: Chlorhexidine gluconate 0.1% and povidone iodine 1% were effective in reducing the growth of Streptococcus mutans bacteria.

The influence of chlorhexidine gluconate and povidone iodine on the minimum inhibitory cencentration and quantitative of streptococcus mutans Apriani, Anie; Mandalas, Henry Yonatan; Kurnia, Calvin; Rahmawati, Dicha Yuliadewi; Lison, Thio; Mulyadi, Nabila
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.%p

Background: An imbalance in the normal flora of the oral cavity can cause dental caries. The prevalence of caries in primary teeth in Indonesia which experiences caries is 90.2%, which shows that the prevalence of caries is still quite high. One way to prevent bacterial activity is using mouthwash. Chlorhexidine gluconate and Povidone Iodine are antibacterial agents that are effective in inhibiting bacterial growth, through bacteriostatic and bactericidal actions. This study aims to determine whether there is an inhibitory effect of chlorhexidine gluconate and povidone iodine on the growth of Streptococcus mutans (quantity).Method: This study used the broth microdilution method by treating Streptococcus mutans using 0.1 % chlorhexidine gluconate and 1% povidone iodine. The effect of inhibitory power was measured using the broth microdilution technique and the total plate count technique in the form of Colony Forming Units (CFU). Statistical calculations use the Shapiro-Wilk normality test, Levene's homogeneity test and Tukey's Post Hoc test to determine differences between treatments.Result: The results of the research showed that the treatment group in the form of 0.1% chlorhexidine gluconate and 1% povidone iodine had a very significant effect on the growth of bacterial colonies, which was very significant for the growth of Streptococcus mutans bacterial colonies.Conclusion: Chlorhexidine gluconate 0.1% and povidone iodine 1% were effective in reducing the growth of Streptococcus mutans bacteria.

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