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All Journal Jurnal Veteriner International Journal of Science and Engineering (IJSE) AL KAUNIYAH Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Peternakan Indonesia BIOMA Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Veteriner
Sunarno Sunarno
Biology Laboratory Structure And Function Of Animals, Department Of Biology, Faculty Of Science And Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH, Tembalang, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Suplemen kombucha dan kayu manis untuk menghasilkan daging broiler kaya antioksidan dan rendah kolesterol Laili Fitria Zulfa; Sunarno Sunarno; Salsabila Alifah; Salindri Prawitasari
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.503 KB) | DOI: 10.14710/jbt.2.1.34-40

Abstract

Quality and healthy meat is the goal of broiler cultivation. One way to get quality, safe broiler meat is by giving kombucha tea and cinnamon flour supplements in a diet that can lower cholesterol and increase the antioxidant content of meat. This study aims to analyze the cholesterol and antioxidant content of broiler (femoris and  pectoralis major muscles) after the supplementation of kombucha tea and or cinnamon flour in feed. This study uses a completely randomized design consisting of 5 treatments with      4 replications. The treatment in this study included P0: control, without being given kombucha tea and cinnamon flour, P1: 5% cinnamon flour; P2, P3, and P4 respectively are 100% kombucha tea and 5% cinnamon flour with a ratio (3: 1), (1: 1), and (1: 3). The variables measured were total antioxidant and cholesterol levels in the femoral and pectoral major muscles. The research data were analyzed by ANOVA followed by Duncan Test with a confidence level of 95%. The results showed that kombucha tea and cinnamon flour supplements significantly affected antioxidant levels and total cholesterol in broiler meat. The conclusion of this study is that 100% kombucha tea with 5% cinnamon with a ratio (3: 1) and (1: 1) gives the most optimal influence on increasing antioxidant levels and decreasing cholesterol levels in femoral muscle and major pectotalis in broilers.
Aplikasi larutan EMOVA daun kelor (Moringa oleifera) dan daun afrika (Vernonia amygdalina) dalam menekan mortalitas ikan nila (Oreochromis niloticus) pada simulasi transportasi Sunarno Sunarno; Eko Purnomo; Khunfaya Firnanda Putri Rossida; Maulida Aqlinia; Falasifah Falasifah; Mamluatul Lailiyah
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.1 KB) | DOI: 10.14710/jbt.2.1.8-15

Abstract

Fish transportationis logistical aspect of the fishery business. Wrong procedures in handling fish during transportation can trigger stress. Herbal ingredients such as kelor (Molinga oleifera) and afrika leaf (Vernonia amygdalina) have the potential to overcome stress on fish. Both types of herbal ingredients contain compounds of flavonoids and phenols that have the potential to be analgesik. The purpose of this study was to analyze the analgesik effect of kelor and afrika leaf extract to nila fish (Oreochromis niloticus) using transportation simulation model. The experimental design was usedcomplete randomized design with four treatments, including the cold temperature of 10ºC (L0), the extract solution of kelor and afrika leaf was use ratio 1:1 with concentrations of 10% (L1), 20% (L2) and 30% (L3) solution. The main parameters observed were viability and mortality. The experiment results showed that the treatment of L1 and L2 was not significantly different with the control, L2 treatment was not significantly different with L3 but L3treatment was significantly different with kontrol and L1. The viability of the L3 treatment is higher than control and treatment of L1, as well as for mortality parameters. The conclusion of this research is, extract of kelor and afrika leaf combination with 1:1 ratio at 30% concentration in transportation simulation model can cause high viability value and able to depress mortality. The results of this study can be used as a solution to fish mortality due to stress and handling of fish based on animal welfare principle during transportation.
Aplikasi biomaterial aktif dari daging ikan gabus (Channa striata) untuk penyembuhan luka pascaoperasi pada hewan model tikus Wistar Sunarno Sunarno; Rahayu Damayanti; Alfisa Devi; Muhammad Fikri; F Pratiwi; Linda Ayu
Jurnal Biologi Tropika Vol.1, No. 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.757 KB) | DOI: 10.14710/jbt.1.2.13-20

Abstract

One problem for postoperative patients is tissue recovery takes a long time due to the occurrence of blood clotting disorders and immune system. Snakehead fish was known to contain bioactive ingredients that can potentially speed up the process of tissue repair as a result of injuries. Snakehead fish meat contains a lot of minerals, albumin, and amino acids needed for tissue protein synthesis and increases the antioxidant glutathione. This study was conducted to test the extraction of snakehead fish flesh against skin tissue wound healing in Wistar rats. The study was conducted with a completely randomized design consisting of three treatments with 6 replication, which include (P0) a negative control (without treatment madecassol and snakehead fish meat extract), (P1) madecassol commercial drug treatment, and (P2) treatment of snakehead fish meat extract. Each material was injected intramuscularly in Wistar rats at a dose of  9 ml/kg bb for 7 days. Data analysis uses Anova followed by test Honestly Significant Difference (HSD) with a significance of 95%. Treatment of snakehead meat extract with a dose of 9 ml/kg bb a significant effect on skin tissue repair after injury, better than treatment with madecassol and control with the average value of extensive tissue remaining after recovering from the condition of the wound by 0.2 cm2; 0.33 cm2; and 0.4 cm2, respectively. Snakehead fish meat extract with a dose of 9 ml/kg bb injected intramuscularly can accelerate the recovery of tissue in the skin after the wound in Wistar rats. 
Jumlah leukosit, persentase limfosit dan persentase monosit ayam petelur jantan setelah perlakuan penambahan serbuk daun kelor pada pakan Muhammad Anwar Djaelani; Kasiyati Kasiyati; Sunarno Sunarno
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.166 KB) | DOI: 10.14710/niche.3.1.45-49

Abstract

This study aimed to observe the effect of Moringa leaf powder as a feed addition to the number of leukocytes, the percentage of lymphocytes and the percentage of monocytes monocytes in chicken. This study used a Completely Randomized Design consisting of five treatments, one treatment group and four treatment groups with basic feed and basic feed which were added with some levels of Moringa leaf powder. Treatment group P0: Basic feed, without the addition of Moringa leaf powder as a control. Treatment group P1: Basic feed added with 1% Moringa leaf powder. P2 treatment group: Basic feed given 2% Moringa leaf powder. P3 treatment group: Basic feed given with additional Moringa leaf powder 3%. P4 treatment group: Basic feed given 4% Moringa leaf powder. The results showed that Moringa leaf powder as an additional feed did not significantly influence the number of leucocyte, percentage of lymphocites and percentage of monocytes among treatment group (P> 0.05). The conclusion of this study is that the addition of Moringa leaf powder to the feed of chicken has the potential to maintain the profile of white blood at normal values, which is indicated by the number of leukocytes, the percentage of lymphocytes and the percentage of monocytes.
Effect of rosella-based kombucha tea on the lipid profile on hyperlipidemic rats (Rattus norvegicus) Ahmad Edi Darmawan; Sunarno Sunarno; Vinsensius M Dhani; Garin F Fairuzzaki
NICHE Journal of Tropical Biology Vol. 1, No. 2, Year 2018
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.761 KB) | DOI: 10.14710/niche.1.2.42-47

Abstract

The purpose of this study was to determine the lipid profile of white rats (Rattus norvegicus) with high-fat feed after fermentation of rosella kombucha with various concentrations. This study used 20 white male rats (Rattus norvegicus) aged two months, with rosella kombucha treatment fermented for five days at 25oC per oral. This study used Completely Randomized Design with four treatments (for 21 days) and four replications, namely: P0 = Control, rats were only given food and drink as usual (with the same type of rat feed). P1 = 1 ml of pork oil/day for 14 days + 1.8 ml of rosella kombucha 50% in the morning and evening for seven days. P2 = 1 ml of pork oil/day for 14 days + 1.8 ml of 75% rosella kombucha morning and afternoon for seven days. P3 = 1 ml of pork oil/day for 14 days + 1.8 ml of 100% kombucha rosella morning and afternoon for seven days. The variables measured were levels of LDL (Low-Density Lipoprotein) and HDL (High-Density Lipoprotein). The data obtained were analyzed using ANOVA followed by Duncan Test with 95% confidence level using SPSS 16.0 software. The results showed that administration of rosella kombucha did not have a significant effect on LDL and HDL levels of white rats (Rattus norvegicus). Based on the results of the study, it can be concluded that the 5-day fermentation of kombucha rosella with various concentrations did not affect LDL and HDL levels. 
Bobot Lemak Abdominal Ayam Pedaging setelah Pemberian Teh Kombucha dalam Air Minum Rd Yudhi Tresnadi Kusumah; Sri Isdadiyanto; Sunarno Sunarno
Buletin Anatomi dan Fisiologi Volume 2, Nomor 2, Tahun 2017
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.2.2.2017.140-147

Abstract

Teh kombucha merupakan minuman hasil fermentasi teh dan gula oleh bakteri Acetobacter xylinum dan khamir Saccharomyces yang mengandung berbagai jenis asam organik dan vitamin, serta berperan sebagai probiotik. Peran teh kombucha sebagai growth promoter adalah dengan menyempurnakan proses metabolisme dalam pencernaan ayam pedaging, sehingga nutrisi dapat terserap dan tercukupi dengan baik untuk pertumbuhan dan perkembangan yang optimal. Tujuan dari penelitian ini adalah untuk menganalisis bobot lemak abdominal pada ayam pedaging (Gallus gallus) setelah pemberian teh kombucha dalam air minum. Penelitian ini dilaksanakan pada bulan September sampai Oktober 2014 di Laboratorium Biologi Struktur dan Fungsi Hewan. Teh kombucha yang digunakan merupakan hasil fermentasi teh hijau selama 12 hari. Hewan uji 20 ekor ayam pedaging strain CP 707 dibagi acak dalam 4 perlakuan konsentrasi yaitu, 0%, 10%, 20%, dan 40% teh kombucha dalam air minum selama 32 hari. Analisa statistik menggunakan ANOVA pada taraf kepercayaan 95% dan uji lanjut Duncan menunjukkan hasil tidak berbeda nyata terhadap lemak abdominal dan konsumsi pakan, sedangkan bobot tubuh dan konsumsi minum menunjukkan hasil berbeda nyata. Kesimpulan dari penelitian ini adalah pemberian teh kombucha dalam air minum sampai konsentrasi 40% belum mampu menurunkan kandungan lemak abdominal pada ayam pedaging.   Kata kunci: teh kombucha; ayam pedaging; lemak abdominal
Nilai Haugh Unit (HU), Indeks Kuning Telur (IKT) dan Ph Telur Itik Pengging Setelah Pemberian Tepung Daun Kelor (Moringa oleifera Lam.) Susan Nuraeni; Muhammad Anwar Djaelani; Sunarno Sunarno; Kasiyati Kasiyati
Buletin Anatomi dan Fisiologi Volume 4, Nomor 2, Tahun 2019
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.81 KB) | DOI: 10.14710/baf.4.2.2019.107-115

Abstract

Itik lokal banyak dibudidayakan sebagai penghasil telur. Daun kelor (Moringa oleifera) mengandung nutrisi esensial, seperti asam amino dan protein yang berpotensi meningkatkan kualitas telur, terutama telur itik. Penelitian ini bertujuan untuk menganalisis pengaruh tepung daun kelor terhadap kualitas fisik telur itik pengging. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan dengan 4 ulangan, yaitu kelompok kontrol dan kelompok perlakuan pakan basal dengan tambahan tepung daun kelor 2,5; 5; 7,5; dan 10%. Parameter yang diamati pada penelitian ini yaitu nilai Haugh unit (HU), indeks kuning telur (IKT), dan pH telur. Sampel diambil setiap hari kemudian dikumpulkan dan dilakukan pengukuran. Data yang diperoleh dianalisis mengunakan analysis of variance (ANOVA), apabila terdapat beda nyata antar perlakuan dilakukan uji lanjut dengan uji Duncan pada taraf kepercayaan 95%. Hasil analisis menunjukan bahwa pemberian tepung daun kelor dalam pakan itik memberikan pengaruh yang nyata (P<0,05) pada nilai HU dan pH putih telur, namun tidak berpengaruh (P>0,05) pada IKT, pH kuning telur, dan bobot telur. Berdasarkan nilai HU, telur pada penelitian ini dikategorikan dalam kualitas AA, dan pH putih telur tertinggi terdapat pada perlakuan 10% dengan nilai 8,66. Berdasarkan penelitian tersebut dapat disimpulkan bahwa pemberian tepung daun kelor 7,5% dapat meningkatkan kualitas telur tanpa merubah bobot telur. Kata kunci: Itik pengging, daun kelor, kualitas telur
Pengaruh Suplementasi Tepung Daun Kelor (Moringa oleifera Lam.) untuk Mendukung Produksi Telur Itik Pengging (Anas platyrhyncos) Siti Murni Mas&#039;adah; Kasiyati Kasiyati; Muhammad Anwar Djaelani; Sunarno Sunarno
Buletin Anatomi dan Fisiologi Volume 5, Nomor 1, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.1.2020.25-34

Abstract

Daun kelor mengandung berbagai macam asam amino esensial dan kaya akan antioksidan. Penelitian ini bertujuan untuk menguji pengaruh tepung daun kelor terhadap produksi telur itik pengging. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas 5 perlakuan dengan 4 kali ulangan dan setiap ulangan berisi 3 ekor itik, yaitu kelompok kontrol dan kelompok perlakuan pakan basal yang diberi imbuhan tepung daun kelor 2,5; 5; 7,5; dan 10%. Pengukuran variabel penelitian dilakukan setiap satu minggu dan diukur selama tiga minggu. Data dianalisis dengan ANOVA satu arah yang dilanjutkan dengan uji Duncan pada taraf kepercayaan 95%. Hasil penelitian menunjukan bahwa imbuhan tepung daun kelor pada pakan itik pengging memberikan pengaruh yang signifikan pada produksi telur (p<0,05). Imbuhan tepung daun kelor dengan konsentrasi 2,5 dan 10% pada itik pengging mampu meningkatkan persentase produksi telur duck day, masing-masing sebesar 45,34% dan 39,71% dibandingkan kelompok kontrol (tepung daun kelor 0%). Hasil analisis kualitas kerabang telur untuk warna dan ketebalan pada setiap perlakuan tidak berbeda nyata (p>0,05), sedangkan untuk bobot kerabang menunjukan hasil yang signifikan (p<0,05). Kesimpulan penelitian ini adalah imbuhan tepung daun kelor dengan konsentrasi 2,5 dan 10% pada pakan itik pengging dapat meningkatkan produksi telur tanpa merubah kualitas kerabang telur. 
Somatometri Tulang Ekstremitas Ayam Jantan Setelah Pemberian Tepung Daun Kelor (Moringa oleifera) Sebagai Pakan Tambahan Kencana Ayudya Prabahandari; Kasiyati Kasiyati; Muhammad Anwar Djaelani; Sunarno Sunarno
Buletin Anatomi dan Fisiologi Volume 6, Nomor 2, Tahun 2021
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.6.2.2021.183-192

Abstract

 Daun kelor (Moringa oleifera) merupakan bagian tanaman yang mengandung nutrisi pendukung pertumbuhan tulang ekstremitas ayam jantan, meliputi protein dan mineral kalsium. Atas dasar potensi tersebut, dilakukan penelitian yang bertujuan untuk menganalisis pakan tambahan tepung daun kelor pada pertumbuhan tulang ekstremitas ayam jantan. Parameter uji penelitian ini meliputi panjang sayap, panjang tibiotarsus, panjang tarsometatarsus, panjang badan, dan panjang paruh. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas 5 perlakuan meliputi P0 (pakan standar 100%), P1, P2, P3 dan P4 berturut-turut mendapat tambahan tepung daun kelor 1%, 2%, 3%, dan 4% dengan pakan standar 99%, 98, 97%, dan 96%, tiap perlakuan diulang 3 kali. Data dianalisis menggunakan Analysis of Variance (ANOVA) dan uji lanjut Duncan Multi Range Test(DMRT) (P<0,05). Hasil penelitian didapatkan bahwa pemberian tepung daun kelor berpengaruh signifikan terhadap pertumbuhan panjang sayap dan panjang tarsometararsus, namun tidak berpengaruh signifikan terhadap pertumbuhan panjang tibiotarsus, panjang badan, dan panjang paruh. Kesimpulan dari penelitian ini adalah penambahan tepung daun kelor pada pakan berpotensi meningkatkan panjang tulang sayap dan tulang tarsometatarsus yang merupakan bagian dari tulang ekstremitas ayam jantan Moringa leaf (Moringa oleifera) is part of plant that contains nutrients that support rooster extremity bone growth, including protein and calcium minerals. Base on this potential, doing research to analyzing  effect of supplemented Moringa meal on growth of rooster extremity bones. The test parameters of this study include wing length, tibiotarsus length, tarsometatarsus length, body length, and beak length. The research used completely randomized design (CRD) which consisted of 5 treatments including P0 (standard feed 100%), P1, P2, P3, and P4, respectively, received moringa leaf additions of 1%, 2%, 3%, and 4% with standard feed of 99%, 98, 97%, and 96%, each treatment was repeated 3 times. Data were analyzed using Analysis of Variance (ANOVA) and Duncan Multi Range Test (DMRT) advanced test (P <0.05). The results showed that supplementation of Moringa meal was significantly different (P<0.05) of the wing and tarsometatarsal length. However, it was not significantly different (P>0.05) in tibiotarsal length, body length, and beak length. Supplementation of Moringa leaf meal on the diet of rooster did not increase extremity bones at grower phase. The conclusion was addition of Moringa leaf meal in diet potentially increase wing and tarsometatarsal bone length which are part of rooster extremity bones.
Pengaruh Imbuhan Tepung Daun Kelor (Moringa oleifera Lam.) pada Pakan terhadap Bobot Beberapa Organ Dalam dan Lemak Abdominal Itik Pengging (Anas platyrhyncos) Dara Puspita Jati Windoro; Kasiyati Kasiyati; Muhammad Anwar Djaelani; Sunarno Sunarno
Buletin Anatomi dan Fisiologi Volume 5, Nomor 2, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.2.2020.109-118

Abstract

Daun kelor (Moringa oleifera Lam.) mengandung antioksidan, vitamin C, vitamin A, kalsium, protein, dan berbagai macam asam amino. Semua kandungan nutrien tersebut berperan penting dalam menunjang produktivitas itik pengging, baik daging maupun organ dalam. Penelitian ini bertujuan untuk menganalisis pengaruh imbuhan tepung daun kelor pada pakan terhadap bobot organ dalam dan lemak abdominal itik pengging. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas 5 perlakuan dengan 3 kali ulangan, yaitu kelompok kontrol dan perlakuan pakan basal yang diberi suplemen tepung daun kelor 2,5; 5; 7,5 dan 10%. Pemberian pakan dan minum dilakukan secara ad libitum. Data dianalisis dengan pola distribusi normal dan homogenitas data, kemudian untuk mengetahui perbedaan antarkelompok, data dianalisis dengan ANOVA-satu arah. Hasil penelitian menunjukkan bahwa imbuhan suplemen tepung daun kelor pada pakan itik pengging tidak berpengaruh signifikan (P>0,05) terhadap bobot jantung, limpa, lambung, dan lemak abdominal itik pengging. Kesimpulan dari penelitin ini adalah imbuhan tepung daun kelor pada pakan tidak meningkatkan bobot jantung, limpa, dan lambung, serta tidak menurunkan lemak abdominal itik pengging. Kata kunci: daun kelor, jantung, limpa, lambung, lemak abdominal, itik pengging.
Co-Authors A. P. Nagari Adhitya Yoga Pradana Riyadi Ahmad Edi Darmawan Ahmad Rifai Alfisa Devi Amni Z. Rahman Annisa Widyasari Aziza Rizky Safitri Bayu Pradika Beta Cahyaning Nugraheni Bima Adistya Dara Puspita Jati Windoro Dwi Rahmawati Eko Purnomo Endang Nawarikita Evi Lestari F Pratiwi Falasifah Falasifah Fendy E. Wahyudi Garin F Fairuzzaki Hikmah Putri Pratiwi K. Budiraharjo Kasiyati Kasiati Kencana Ayudya Prabahandari Khunfaya Firnanda Putri Rossida Khunfaya Firnanda Putri Rossida Kusuma Alya Fathurika Laili Fitria Zulfa Linda Ayu Mamluatul Lailiyah Maulida Aqlinia Mohammad Farhan Aditya Muhammad Adnan Jafar Alfian Muhammad Alvin Gibran Muhammad AmmarNurHandyka Muhammad Anwar Djaelani Muhammad Fikri Muhammad Fikri Zulfikar Novia Marcelina Rahayu Damayanti Rd Yudhi Tresnadi Kusumah Rully Rahadian Salindri Prawitasari Salsabila Alifah Salsabila Alifah Satwika Paramasatya, Satwika Silvana Tana Siska Hairunnisa Siti Muflichatun Mardiati Siti Murni Mas&#039;adah Siti Murni Mas&#039;adah Solikhin Solikhin SRI ISDADIYANTO Sri Isdadiyanto Sri Pujiyanto Sri Widodo Agung Suedy Susan Nuraeni Vinsensius M Dhani Widiartanto Widiartanto