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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat BERITA BIOLOGI Sains Natural: Journal of Biology and Chemistry Buletin Penelitian Tanaman Rempah dan Obat
Otih Rostiana
Balai Penelitian Tanaman Obat dan Aromatik
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Immunology & microbiology Industrial & Manufacturing Engineering Veterinary

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Journal : BERITA BIOLOGI

 1 
PENGARUH 2,4-D DAN BA TERHADAPINDUKSIKALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE {Zingiber officinale Rose.) Sitinjak, Rama Riana; Rostiana, Otih; Karyono, Karyono; Supriatun, Titin
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i2.2037

Abstract

The aim of this study was to evaluate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and N -benzyladenin (BA) to embryogenic callus induction of meristem culture in ginger {Zingiber officinale Rose). Completely Randomixed Design was applied using factorial pattern, replicated 3 times. Two factors were assigned i.e: 2,4-D (1.0, 2.0 and 3.0 mg/L) and BA (0.0, 1.0, 3.0 and 5.0 mg/L). Result showed that 2,4-D and BA significantly affected the embryogenic callus induction. The highest embryogenic callus (93.33%) was achieved on MS (Murashige & Skoog) containing combination of 1.0 mg/L 2,4-D and 3.0 mg/L BA, after 8weeks incubation.Histological evaluation informed that the incubation of embryogenic calli started from cortex cells adjacent the meristem vascular tissues.
PENGARUH MEDIA DASAR MS DAN N TERHADAP PERKEMBANGAN EMBRIO 6 SOMATIK PADA KULTUR MERISTEM JAHE (Zingiber officinale Rosc.) Rostiana, Otih; Syahid, Sitti Fatimah
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.828 KB) | DOI: 10.14203/beritabiologi.v8i5.1899

Abstract

This study was performed to evaluate the development of somatic embryo from embryogenic calli of ginger meristem culture. Completely randomized design was applied, replicated 4 times. Embryogenic calli from meristem tissue of inner shoot bud of rhizome obtained on MS medium containing 100 mg/L glutamine, 2% sucrose with the addition of 1.0 mg/L 2,4-D and 3.0 mg/L BA, were subjected to proliferation medium, MS and N basal media containing 3% mannitol. Then, transferred into somatic embryo maturation medium, either MS or N basal media supplemented with 6% sucrose. The number of somatic embryos-formed significantly affected by the proliferation medium applied. The highest number of somatic embryos (about 82.0 per 1 g friable calli) was achieved on the MS medium, 4 weeks after incubation. In addition, the optimum growth of embryogenic calli containing somatic embryos was obtained on MS and N medium supplemented with 6% sucrose. There were significantly difference between the media applied (MS and N ) to somatic embryos maturation. The highest number of mature somatic embryos (57.2 embrios) was achieved on the MS medium, 18 days after incubation.
Co-Authors . Subaryanti Abdul Qadir Agus Ruhnayat Deliah Seswita Karyono Karyono Mohamad Rahmad Suhartanto Natalini Nova Kristina Rama Riana Sitinjak Rosita SMD Sitti Fatimah Syahid Tias Arlianti Titin Supriatun