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All Journal HAYATI Journal of Biosciences Majalah Kedokteran The Indonesian Biomedical Journal Journal of Medicine and Health
Djaja Rusmana
Universitas Kristen Maranatha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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In Vitro Antimicrobial Activity of Javanese Tamarind Leaves Infusion (Tamarindus indica Linn.) in Escherichia coli Suryadi, Caroline; Rusmana, Djaja; Evacuasiany, Endang
Journal of Medicine and Health Vol 1, No 1 (2015)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Escherichia coli is the most common bacteria that cause diarrhea. Based on Kurniawati’s research, ethanol extract of tamarind leaves has an antimicrobial effect to Escherichia coli. This study aims to determine the antimicrobial effect using disk diffusion method, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of tamarind leaves infusion against Escherichia coli. This research was done using a real laboratory experimental design with tamarind leaves infusion and Escherichia coli microbes as the research objects. Zones of inhibition were observed using disk diffusion method by putting the tamarind leaves infusion disks on Mueller-Hinton agars with Ampicillin antibiotic for comparison, followed by observing the MIC using macro broth dilution method  and MBC. The result of disk diffusion showed that tamarind leaves infusion had no zone of inhibition, whereas the mean value of the zones of inhibition of ampicillin is 17.2 mm. The MIC of tamarind leaves infusion is 62.5 mg/mL. Average growth of bacteria  for the MBC of tamarind leaves infusion at 125 mg/mL and 62.5 mg/mL concentration are 13 CFU/mL and >300 CFU/mL. It can be concluded that tamarind leaves infusion has antimicrobial activity effect against E.coli and that effect is bacteriostatic. Keywords: Tamarindus indica Linn, Escherichia coli, MIC, MBC
Antioxidant Activity of Phyllanthus niruri Extract, Rutin and Quercetin Djaja Rusmana; Roro Wahyudianingsih; Mariska Elisabeth; Balqis Balqis; Maesaroh Maesaroh; Wahyu Widowati
The Indonesian Biomedical Journal Vol 9, No 2 (2017)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v9i2.281

Abstract

BACKGROUND: Normal metabolism of oxygen and exogenous factors constantly generate free radicals which could be harmful to the human body. Human need antioxidants to provide protection against free radicals, thus plants are a good source of natural antioxidants. Phyllanthus niruri (P. niruri) has been known to possess several medicinal properties and contain numerous active phytochemical. In this research, we conducted phytochemical screening and antioxidant assay of P. niruri extract along with the compounds rutin and quercetin, which are flavonoids possessing medicinal properties. This study was conducted to determine P. niruri, rutin and quercetin as antioxidant.METHODS: In this study, qualitative phytochemical screening was performed to detect phenol, flavonoid, saponin, tannin, steroid/triterpenoid, terpenoid and alkaloid in P. niruri extract. Antioxidant analysis of P. niruri, rutin and quercetin was conducted using total measured phenolic content, 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2’-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays.RESULTS:  The study revealed that P. niruri extract contained saponin, phenol, flavonoid and tannin based on phytochemical screening. In DPPH and ABTS assays quercetin possessed highest antioxidant activity with IC50 value of 0.55 and 1.17 μg/ml respectively. Meanwhile, P. niruri extract showed the highest FRAP activity which was 373.95 μM Fe(II)/μg extract. Rutin possessed the lowest antioxidant activity in all antioxidant assays.CONCLUSION: This study confirmed that P. niruri extract and quercetin have great potential as a natural antioxidant source.KEYWORDS: asntioxidant, phytochemical, Phyllanthus niruri, quercetin, rutin, free radical
Efek Antimikroba Infusa Biji Pinang (Areca catechu Linn.), Daun Sirih (Piper betle Linn) dan Kombinasinya Terhadap Staphylococcus aureus Secara In Vitro Rusmana, Djaja; Gosandra, Sherrina O.; Widowati, Wahyu
Majalah Kedokteran UKI Vol. 37 No. 2 (2021): MEI - AGUSTUS
Publisher : Fakultas Kedokteran Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/mk.v37i2.4821

Abstract

AbstrakStaphylococcus aureus menjadi bakteri penyebab utama terjadinya abses di rongga mulut. Frekuensi dental abses di dunia mulai dari 0,7% sampai 15% dan 14% di Indonesia. Biji pinang (Areca catechu L.) dan Daun sirih (Piper betle L.) memiliki aktivitas antimikroba. Tujuan penelitian ini adalah menilai aktivitas antimikroba dari infusa biji pinang, daun sirih dan kombinasinya terhadap Staphylococcus aureus. Penelitian ini bersifat eksperimental laboratorik metode difusi dengan metode Kirby-Bauer. Penelitian ini dilakukan triplo. Rerata zona inhibisi terbesar terdapat pada ciprofloxacin (25,46±0,19 mm), diikuti infusa biji pinang 6% (11,76±0,96 mm), 8% (11,60±0,64 mm), 10% (11,38±0,96 mm), kombinasi infusa biji pinang 6% dan daun sirih (10,88±0,72mm), infusa daun sirih (9,82±0,81 mm), kombinasi infusa biji pinang 8% dan daun sirih (8,66±0,41 mm), dan kombinasi infusa biji pinang 10% dan daun sirih (8,54±1,23 mm). Simpulan penelitian infusa biji pinang 6%, 8%, 10%, infusa daun sirih, dan kombinasinya memiliki efek antimikroba terhadap S. aureus.Kata Kunci: biji pinang; daun sirih; antimikroba; Staphylococcus aureus AbstractStaphylococcus aureus is the main cause of abscesses in the oral cavity. The frequency of dental abscesses in the world ranges from 0.7% to 15% and 14% in Indonesia. Areca nut (Areca catechu L.) and Betel leaf (Piper betle L.) has antimicrobial activity. The purpose of this study was to measure the inhibition zone from infusion of areca nut, betel leaf and their combination against Staphylococcus aureus and is expected to be a complementary therapy. This research is a laboratory experimental study using the Kirby-Bauer method of diffusion. This research was conducted in triplo. The largest mean zone of inhibition was found in ciprofloxacin (25.46±0.19 mm), followed by areca nut infusion 6% (11.76±0.96 mm), 8% (11.60±0.64 mm), 10% (11.38±0.96 mm), combination of betel leaf infusion and 6% areca nut (10.88±0.72mm), betel leaf infusion (9.82±0.81 mm), combination of betel leaf and 8% areca nut (8.66±0.41 mm), and combination of 10% areca nut infusion and betel leaf (8.54±1.23 mm). The conclusion of this research was that betel nut infusion 6%, 8%, 10%, betel leaf infusion, and their combination have antimicrobial effect against S.aureus.Keywords: areca nut; betel leaf; antimicrobial; Staphylococcus aureus*DR: Penulis Koresponden; Email: jayarusmana67@gmail.com
Potential of Clitoria ternatea L. Extract Towards Insulin Receptor Expression and Marker of Inflammation in Diabetes Mellitus Rats Model Rusmana, Djaja; Tiono, Hartini; Widowati, Wahyu; Lucianus, Johan; Darsono, Lusiana; Onggowidjaja, Philips; Tjokropranoto, Rita; Vera, Vera; Setiabudi, Edwin; Obeng, Selonan; Kusuma, Hanna Sari Widya; Novianto, Agung
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.803-810

Abstract

Metabolic abnormalities caused by the accumulation of human, environmental, genetic and lifestyle variables can be found in diabetes mellitus (DM). An increase in blood glucose carried on by a reduction in insulin production can also result in DM. Insulin resistance often occurs as a result of obesity and a lack of physical activity and aging. Telang flower extract (Clitoria ternatea L.) is reported to have several qualities such as being able to treat DM, chronic bronchitis, goiter, mucosal disorders and leprosy. This study’s objective was to elucidate anti-dibetic effect of C. ternatea extract (CTE) in rats with diabetic complications related to dyslipidemia. Rats suffering from diabetes will be examined after being given oral doses of Simvastatin 0.9 mg/kg BW, Glibenclamide 0.45 mg/kg BW, and 200, 400, and 800 mg/kg BW CTE, model induced by streptozotocin. The expression of the insulin gene (INS-1) was investigated by qRTPCR, Tumor Necrosis Factor-α (TNF-α), and Interleukin-1β (IL-1β) pancreatic DM rats model using the Immunohistochemistry (IHC) test. Both descriptive and quantitative data were acquired for the data. After utilizing ANOVA to evaluate quantitative data, the Tukey post hoc test was used to analyze the data, the expression of TNF-α and IL-1β was found to decrease while INS-1 expression increased in response to CTE. This effect was attributed to the modulation of TNF-α, IL-1β, and INS-1 expression. These findings suggest that CTE possesses antidiabetic properties.
Co-Authors Agung Novianto, Agung Balqis Balqis Caroline Suryadi, Caroline Edwin Setiabudi Endang Evacuasiany Gosandra, Sherrina O. Hanna Sari Widya Kusuma, Hanna Sari Widya Hartini Tiono Johan Lucianus Lusiana Darsono Maesaroh Maesaroh Mariska Elisabeth Obeng, Selonan Philips Onggowidjaja Rita Tjokropranoto Roro Wahyudianingsih vera vera, vera WAHYU WIDOWATI Wahyu Widowati