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All Journal Jurnal Peternakan Indonesia YARSI Medical Journal
Fransiska RZ
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Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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BIOSINTESIS ANTIGEN PERMUKAAN HEPATITIS B “HBSAG100” PADA ESCHERICHIA COLI DALAM RANGKA PRODUKSI PROTEIN REKOMBINAN SEBAGAI MODEL IMUNOGEN UNTUK MENGHASILKAN ANTIBODI Riyadi, Slamet; RA Maheswari, Rarah; Sudarwanto, Mirnawati; RZ, Fransiska; Ali, Muhamad
Jurnal Kedokteran YARSI Vol 18, No 2 (2010): MEI - AGUSTUS 2010
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.94 KB) | DOI: 10.33476/jky.v18i2.188

Abstract

Biosintesis protein rekombinan melalui Escherichia coli memberikan alternatif untuk menghasilkan protein antigen yang bermanfaan bagi kepentingan kesehatan yang bebas dari protein manusia. Penelitian ini menggabungkan fragmen DNA dari antigen permukaan virus Hepatitis B dengan gen penyandi enzim gluthation-S-transferase (GST) di dalam plasmid p GEX-4T-2 yang di ekspresikan di dalam sel-sel Escherichia coli. Polypeptida dengan berat molekul sekitar 34,8 kDa telah diproduksi dan diidentifikasi sebagai protein gabungan GST-HB100. Protein gabungan tersebut kemudian dimurnikan menggunakan kolum GSTrap yang disambung dengan kolum HiTrap. Selanjutnya, protein hasil pemurnian tersebut diharapkan bisa digunakan sebagai bahan vaksin atau untuk menghasilkan antibodi.
Kloning Gen Penyandi Antigen HBsAg100 dalam Rangka Produksi Protein Rekombinan Sebagai Model Imunogen untuk Menghasilkan Antibodi S. Riyadi; R. RA Maheswari; M. Sudarwanto; Fransiska RZ; M. Ali
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 13, No 3 (2011): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.13.3.241-248.2011

Abstract

Since one decade ago, a new paradigm of vaccine design is emerging. Instead of attenuated virulent microorganisms or killed virulent microorganisms, effective subunit vaccines were developed using recombinant DNA technology. By using the technology, selected genes of the virulent microorganisms can be cloned, expressed, and evaluated as vaccine components. In this research, hydrophilic domain of S protein (aa 100-164)-encoding gene of hepatitis B surface antigen was cloned for vaccine candidate production. The gene was ligated with pGEX-4T-2 vector and sequenced. Sequences aligment of the amplified fragment with genome of hepatitis B virus indicated that the sequences were identical. A major result achieved from this research was clones carrying S antigens-encoding gene that could be used further for production of recombinant hepatitis B vaccine candidates.
Co-Authors M. Ali M. Sudarwanto MIRNAWATI SUDARWANTO Muhamad Ali R. RA Maheswari RA Maheswari, Rarah S. Riyadi Slamet Riyadi