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All Journal Media Veteriner Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia
M. Sudarwanto
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Nugroho, Widagdo Sri; Sudarwanto, M.; Lukman, D.W; Setiyaningsih, E.; Usleber, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 4 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.452 KB) | DOI: 10.14334/jitv.v14i4.335

Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces
Agroclimatic effects on milk production and sub-clinical mastitis prevalence in dairy cattle Susanty, H.; Purwanto, B.P.; Sudarwanto, M.; Atabany, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.373-382

Abstract

The present study was done to observe effect of agroclimate on milk production and prevalence of sub-clinical mastitis of local Holstein-Friesian dairy cattle in Indonesian small holder dairy farms. The research side were located in Region of <1000 masl and ≥1000 m above sea level (asl) in west Java. Data were obtained from 133 small holder dairy farms included 336 dairy cows in normal lactation period. Parameters observed were agro-climate condition, feed consumption, milk production and milk quality, and sub-clinical mastitis prevalence. Result sowed that THI at <1000 m asl had a range of 76.1-76.7 leads to mild heat stress, while THI at ≥1000 m asl had a range of 66.2 - 66.8 which makes comfort environment to the dairy cow. Differences in feed consumption (P<0.01), milk production and quality (P<0.05) were found among two altitude study location. Milk production at ≥1000 m asl was 2.3 kg higher than at <1000 m asl. The udder health status was also different, a 51% of total sample at <1000 masl was detected sub-clinical mastitis (SCM) as high criteria, while 42% samples that were collected at ≥1000 m asl detected sub-clinical mastitis as middle criteria. In conclusion, agro-climate condition (THI) affect animal’s comfort, milk production and sub-clinical mastitis prevalence.
The Management Of Water Treatment At The Slaughterhouse Depok-Bogor A.W. Sanjaya; M. Sudarwanto; E.S. Pribadi
Media Veteriner Vol. 3 No. 2 (1996): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.687 KB)

Abstract

RPH Depok yang terletak ditengah pemukiman padat mempunyai aktifitas penyembelihan ternak besar, sapi dan kerbau sebanyak 15-20 ekorlhari. RPH ini sudah dilengkapi dengan unit pengolah limbah sederhana.Untuk limbah padat ditumpuk terbuka dan limbah cair diendapkan dalam 5 buah bak khusus dan satu bak kontrol. Kondisi bangunan bak sebagian telah rusak. Hasil analisis laboratorium limbah cair yang dikeluarkan ke perairan menunjukkan belum ada penurunan kandungan BOD, COD maupun padatan tersuspensi, sedangkan pH limbah cair yang di analisa pada saat jumlah penyembelihan belum banyak, ada penurunan. Hal ini membuktikan bahwa bak pengolah berperan sangat nyata dalam pengolahan limbah cair di RPH tersebut.
Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Widagdo Sri Nugroho; M. Sudarwanto; D.W Lukman; E. Setiyaningsih; E. Usleber
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 4 (2009): DECEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.452 KB) | DOI: 10.14334/jitv.v14i4.335

Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces
Kloning Gen Penyandi Antigen HBsAg100 dalam Rangka Produksi Protein Rekombinan Sebagai Model Imunogen untuk Menghasilkan Antibodi S. Riyadi; R. RA Maheswari; M. Sudarwanto; Fransiska RZ; M. Ali
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 13, No 3 (2011): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.13.3.241-248.2011

Abstract

Since one decade ago, a new paradigm of vaccine design is emerging. Instead of attenuated virulent microorganisms or killed virulent microorganisms, effective subunit vaccines were developed using recombinant DNA technology. By using the technology, selected genes of the virulent microorganisms can be cloned, expressed, and evaluated as vaccine components. In this research, hydrophilic domain of S protein (aa 100-164)-encoding gene of hepatitis B surface antigen was cloned for vaccine candidate production. The gene was ligated with pGEX-4T-2 vector and sequenced. Sequences aligment of the amplified fragment with genome of hepatitis B virus indicated that the sequences were identical. A major result achieved from this research was clones carrying S antigens-encoding gene that could be used further for production of recombinant hepatitis B vaccine candidates.
Co-Authors A. Atabany, A. A.W. Sanjaya B.P. Purwanto, B.P. D.W Lukman E. Setiyaningsih E. Usleber E.S. Pribadi Fransiska RZ M. Ali R. RA Maheswari S. Riyadi Susanty, H. Widagdo Sri Nugroho