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All Journal HAYATI Journal of Biosciences Jurnal Sains Materi Indonesia Majalah Obat Tradisional JFIOnline Jurnal Ilmu Kefarmasian Indonesia
Zuhelmi Aziz
Universitas Pancasila
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : Jurnal Ilmu Kefarmasian Indonesia

 1 
Identifikasi Senyawa Flavonoid dalam Fase n-Butanol dari Ekstrak Metanol Daun Wungu (Graptophyllum pictum (L.) Griff) Zuhelmi Aziz; Wiwi Winarti; Kona Nate
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Wungu leaves is one of the herbal plants in Indonesia that have been used significantly by the society to cure scare, swollen, ulcer, gall stone, abscess, skin diseases, liver, hearing disfunction, hemorrhoids, bloody cough, fever and laxative, isolation and identification of the type of flavonoid substance found in n-butanol phase of Wungu leaves methanol extract have been conducted by employing identification by UV-visible spectrophotometry, the result of the examination showed that isolate NB-IV was presumably considered as flavonol substance with 3-OH (with or without 5-OH), isolate NB-V was presumably considered as antosianidin substance with o-diOH on ring A, isolate NB-VI and isolate NBVII was presumably considered as flavonol substance with OH on position 3,5,7 and o-diOH on ring B.
Aktivitas Sitotoksik dan Profil Kromatogram Daun Sirsak (Annona muricata L.) yang Diiradiasi ERMIN KATRIN; RHILA AMALIAH; ZUHELMI AZIZ; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Soursop (Annona muricata L.) is one of plants included in Annonaceae which the leaves has cytotoxic activity on cancer cells. Preservation efforts of soursop leaves is carried out by gamma irradiation technic. This research aimed to study the effect of gamma irradiation on the bioactivity and chromatogram profiles of the active fraction from soursop leaves.Dry powders of soursop leaves were irradiated using gamma with doses of 5; 7.5; 10; and 15 kGy and repeated twice, respectively for certain doses. Then each sample was macerated with n-hexane, ethyl acetate and ethanol gradiently. Each extract was tested against leukemia L1210 cells. Ethyl acetate extract was the most active (IC50= 7.36 µg/mL) compared with n-hexane (20.18 µg/mL) and ethanol extract (13.89 µg/mL). Ethyl acetate extracts was fractionated using column chromatography obtained ten fractions. The result of cytotoxic activities assay of ten fractions were obtained that fraction 8 was the most active with IC50 value of 0.45 µg/mL. Cytotoxic activity of fraction 8 from irradiated samples showed that gamma irradiation decreased cytotoxic activity of fraction 8, but they were still active (IC50 < 20 µg/mL). Analysis and identification of fraction 8 by the HPLC and TLC-densitometry showed that there were broad peak which decreased in the irradiated samples.The maximum radiation dose for soursop leaves without damaging its bioactivity and that did not change the chromatogram profilesis 7.5 kGy.
Identifikasi Senyawa Penghambat Enzim α-Glukosidase dari Ekstrak Etanol 96% Daun Yakon (Smallanthus sonchifolius [Poepp.& Endl.]H. Robinson) Zuhelmi Aziz; Farhana Hanida Al Qisthi; Ratna Djamil; Nancy Dewi Yuliana; Partomuan Simanjuntak; Syamsudin Abdillah
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 1 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.339 KB) | DOI: 10.35814/jifi.v17i1.652

Abstract

The leaves of yacon (Smallanthussonchi folius [Poepp. &Endl.] H. Robinson) or in Indonesia known as “Daun insulin” (Insulin leaf), scientifically has various medicinal properties and one of them as antidiabetic. The aim of this research is to know α-glucosidase enzyme inhibitor compound in the ethanol 96% extract of yacon leaves. Fractionation of ethanol extract subjected to column chromatography (SiO2; dichloromethane-methanol-water = 5 : 5 : 1) gave 5 fractions (Fr -1 ~Fr – 5). The results of the -glucosidase enzyme inhibition activity test in vitro against the five fractions obtained the 3rd fraction as the most active of 87.84 %. Purification of the 3rd fraction with preparative thin layer chromatography (SiO2; n-BuOH-ethanol-water = 4 : 1 : 2.2) gave pure isolate as Fr 3-1. Identification of pure isolates Fr 3-1 was carried out by interpreted spectra of Ultraviolet, Fourier Transform-Infra Red, Mass Spectra (from LC-MS) and compared data on the chemical shift of the Nuclear Magnetic Resonance (proton and carbon) and determined as Nystose (GF3) compound. The results of the -glucosidase enzyme inhibition activity showed that Fr 3-1 has IC50 of 21.36 ppm
Pengaruh Iradiasi Sinar Gamma dan Konsentrasi Kitosan terhadap Pembentukan Hidrogel Polivinil Alkohol-Kitosan Zuhelmi Aziz; Gatot Trimulyadi; Trivena Fithriany A
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Chitosan is one of the product from the shrimp disposal. Its medicinal and pharmaceutics application are now developed by pharmaceutical industries, i.e as bioplastics and hydrogel. A research about the effect of gamma radiation and chitosan as a raw material for production of polyvinilalcohol has been carried out. PVA-chitosan hydrogel was made from a mixture of composition of 80% PVA in 10% solution and 20% of chitosan in 0, 1, 2 ,and 3% solution respectively. The mixture was heated in oven at 80°C for two hours and then irradiated with 0, 15, 25, and 35 kGy gamma radiation. The physical characteristic of the gel was determined, e.g. colour, swelling degree and gel fraction. The colour of hydrogel more yellow darker to raising of chitosan concentration and radiation dosage. Swelling degree PVA-chitosan hydrogel increased meanwhile chitosan concentration and radiation dosage decreased. In absolute ethanol medium (96%) PVA-chitosan hydrogel was deswelling. The swelling degree PVAchitosan hydrogel decreased with increased gel fraction.
Iradiasi Sediaan Obat Herbal Temu Putih Curcuma zedoaria (Berg) Rosc.: Cemaran Mikroba, Sitotoksisitas dan Profil Kromatogram ERMIN KATRIN W.; EPSI NARULITA; ZUHELMI AZIZ; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Gamma irradiation has been used for preservation of herbal medicines, one of which is temu putih Curcuma zedoaria (Berg) Rosc. to cure cervical cancer. This research aimed to study the gamma irradiation effects on microbial contamination, cytotoxic activity against leukemia L1210 cell lines and chromatogram profile of the temu putih active fraction. Gamma irradiation was performed with a 60Co source at 5, 7.5, 10 and 15 kGy. After irradiation at > 7.5 kGy bacteria and mould were not present on herbs. Control and irradiated samples were consecutively macerated with n-hexane, ethyl acetate and ethanol. Ethyl acetate extract was the most cytotoxic gainst L1210 leukemia cells with an IC50 value of 10.60 μg/mL as compared to n-hexane or ethanol extract. Ethyl acetate extract was fractionated with column chromatography producing six fractions. Fraction 2 was the most cytotoxic against L1210 leukemia cells with an IC50 value of 2.44 μg/mL. The thin-layer chromatographic analysis results of ethyl acetate extract and fraction 2 of control and irradiated samples showed the presence of curcumin and chromatographic pattern similar to the control. Curcumin contents in fraction 2 were between 35 to 51%, which were not significantly changed between samples, although was irradiated up to 15 kGy. The maximum dose for irradiation of temu putih herbal preparation is 15kGy.
Analisis Kandungan Residu Sulfametazin dalam Daging dan Hati Ayam secara Kromatografi Cair Kinerja Tinggi Zuhelmi Aziz; Yulvian Sari; Raphaela Widiastuti; Lydia Septa Desiyana
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 2 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Sulfamethazine is an antimicrobial and anticoccidiocis which usually used in poultry farm. The using of sulfamethazine to cattle has an accumulated residue effect on cattle products. An experiment of the decreasing motive of sulfamethazine residue concentration in chicken meat and liver has been carried out. One day old broiler chicken classified into 4 groups which are negative control, the positive control, the 7 days (7 days before the slaughtering day which on 36th day), and 5 days group (5 days before the slaughtering day which on 36th day). The last three groups are given sulfamethazine solution in oral 3 times a week with 0.2 ml/kg BW or 50 mg/kg BW dosage. After the giving of sulfamethazine stopped, each of those 4 groups slaughtered on 1st, 2nd, 3rd, 4th, 5th, 7th, 24th, 48th, 120th, 168th, 240th hour and then each of chicken meat and liver extracted and analyzed with high performance liquid chromatography (HPLC). The Sulfamethazine residue decreasing along with the increasing of time can be noticed from the experiment. In common, the decreasing of Sulfamethazine can be seen starting form 24th hour, then at 48th hour the sulfamethazine concentration stay at lower state and decrease along with the increasing of time.
Analisis Glikoprotein dalam Daging Mytilus viridis, Anadara granosa, dan Anadara maculosa Zuhelmi Aziz; Thamrin Wikanta; Tirta Subagio
JURNAL ILMU KEFARMASIAN INDONESIA Vol 5 No 1 (2007): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Mussels is known to have ability living in clean or polluted waters environment due to it has a specific immunity system. The immunity mechanism is carried out by a glycoprotein that play role as an immunomodulator with high activity. Extraction of glycoprotein from fresh green mussel (Mytilus viridis), blood mussel (Anadara granosa) and bulu mussel (Anadara maculosa) have been carried out, by boiling the mussels for 30 minutes and 60 minutes, precipitation of glycoprotein with ethanol, then purification of glycoprotein through the Sephadex G-100 column. The amino acids composition of the glycoprotein was analyzed by high performance liquid chromatography (HPLC) with normal phase pico tag column, using 1 ml/min isocratic elution with sodium acetate buffer-acetonitril mixture, and detection with UV detector. Result showed that the yield of glycoprotein from 60 minutes boiling was higher than from 30 minutes boiling. The glycoprotein content of green mussel, blood mussel and bulu mussel were 11,97%, 8,36%, and 18,93% from 60 minutes boiling, and 10,32%, 7,93%, and 9,07 from 30 minutes boiling, respectively. It can be concluded that the amino acids composition in the glycoprotein from both treatment were not proportional , but the 60 minutes boiling was given higher result.
Analysis for quality of senggugu plant and herbal product (Rotheca Serrata (L.) with metabolomics approach Qodriah, Rahmatul; Aziz, Zuhelmi; Damayanti, Volin; Alya, Aura Dyah
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 2 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i2.1670

Abstract

Senggugu plant (Rotheca serrata (L.) has many benefits and has the potential as a raw material for herbal medicine, so an identify in the form of an FTIR fingerprint profile is needed. The purpose of this study was to ensure the quality of simplisia and herbal product of senggugu roots and leaves by metabolomics approach. Simplisia and herbal products powder of senggugu leaves and roots were extracted with 70% ethanol solvent using ultrasonic method. The dried extracts of simplisia and herbal products were analyzed by FTIR and TLC-Densitometry. Data obtained from FTIR and TLC-Densitometry analyzed with multivariate data analysis techniques, Principal Component Analysis (PCA) to obtain fingerprint profiles. The PCA results obtained a total Principal Component value of 98,7% and showed that leaf simplisia and herbal products of senggugu leaves can cluster well, while root simplisia and herbal products of senggugu roots were at a distance from the plot. The analysis with OPLS-DA showed the same fungtional groups, which are C=H, O-H, C=C. The results of antioxidant activity testing of 70% ethanol extracts of leaves, roots, and herbal products of senggugu obtained strong antioxidant activity ability with IC50 values of 80,08; 92,12; 80,53; and 94,03 ppm.
Co-Authors Alya, Aura Dyah Aprillia, Heidy Aprillia, Heidy Damayanti, Volin Dian Pribadi Perkasa EPSI NARULITA Erizal Erizal ERMIN KATRIN ERMIN KATRIN W. Farhana Hanida Al Qisthi Gatot Trimulyadi Hendig Winarno Karomah, Alfi Hudatul Kona Nate Lydia Septa Desiyana M. Rafi Nancy Dewi Yuliana Novi Yantih Partomuan Simanjuntak Purmasari, Davita Qodriah, Rahmatul Raphaela Widiastuti Ratna Djamil RHILA AMALIAH Saputra, Agus Sulistioso G S Syamsudin Abdillah Thamrin Wikanta Tirta Subagio Tohib, Devanka Aulia Trivena Fithriany A Wiwi Winarti Yulvian Sari