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All Journal International Journal of Electrical and Computer Engineering Majalah Ilmiah Teknologi Elektro TELKOMNIKA (Telecommunication Computing Electronics and Control) Physics Student Journal Jurnal EECCIS Journal of Natural A Natural B The Journal of Pure and Applied Chemistry Research Jurnal Fisika Unand ELKOMIKA: Jurnal Teknik Energi Elektrik, Teknik Telekomunikasi, & Teknik Elektronika Science and Technology Indonesia al Kimiya : Jurnal Ilmu Kimia dan Terapan Majalah Ilmiah Teknologi Elektro
Setyawan Purnomo Sakti
Department Of Physics, Brawijaya University, Malang
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Electrical & Electronics Engineering Energy Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Journal : The Journal of Pure and Applied Chemistry Research

 1 
Development of Simple Bacterial Biosensor for Phenol Detection in Water at Medium Concentration using Glass Microelectrode Setyawan Purnomo Sakti; Sri Sugiarti; Unggul Punjung Juswono
The Journal of Pure and Applied Chemistry Research Vol 5, No 1 (2016)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.794 KB) | DOI: 10.21776/ub.jpacr.2016.005.01.219

Abstract

Water is one of the most fundamental natural resources in earth. The availability of clean water becomes a global interest. Many human activities result in water pollution. One from many pollution substances in water is phenol. Phenol is a very common residual compound in industrial activity.  Extensive use of phenol in industry degrades water quality. Regulation has been set in many countries to prevent further damage to the water resource caused by phenol and limiting phenol concentration in water before released into the environment. Therefor it is importance to develop a sensor which can detect phenol concentration in water to be used as a wastewater quality control system. This paper presents a development of bacterial biosensor using Pseudomonas putida and Pseudomonas fluorescens as a biological sensitive material. The sensor was made from glass micro electrode using Ag/AgCl electrode as reference electrode, silver electrode and cellulose ester. The Pseudomonas putida was entrapped inside the nutrient solution and separated by cellulose ester membrane from water containing phenol. It was found that the Pseudomonas putida in used must be growth in 10 hours to reach its optimum growth condition. Linear relationship between biosensor output voltages to phenol concentration was measured for phenol concentration below 200 ppm. The sensitivity of the developed biosensor was 72mV/ppm for Pseudomonas putida and 68.8 mV/ppm for Pseudomonas fluorescens.
Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith Eko Malis; Aprilia Nur Tasfiyati; Elvina Dhiaul Iftitah; Setyawan Purnomo Sakti; Akhmad Sabarudin
The Journal of Pure and Applied Chemistry Research Vol 4, No 2 (2015)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.103 KB) | DOI: 10.21776/ub.jpacr.2015.004.02.214

Abstract

A polymer monolithic column, poly-(methacrylatoethyltrimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) or poly-(MATE-co-VBC-co-EDMA) was successfully prepared in the current study by one-step thermally initiated in situ polymerization, confined in a steel tubing of 0.5 mm i.d. and 1/16” o.d. The monoliths were prepared from methacrylatoethyltrimethylammonium chloride (MATE) and vinylbenzyl chloride (VBC) as monomer and ethylene dimethacrylate (EDMA) as crosslinker using a binary porogen system of 1-propanol and 1,4-butanediol. The inner wall of steel tubing was pretreated with 3-methacryloxypropyl-trimethoxysilane (MAPS). In order to obtain monolith with adequate column efficiency and low flow resistance, some parameters such as total monomer concentration (%T) and crosslinker concentration (%C) were optimized. The morphology of this monolith was assessed by scanning electron microscopy (SEM). The properties of the monolithic column, such as permeability, binding capacity, and pore size distribution were also characterized in detail. From the results of the characterization of all monolith variation, monolith with %T 30 %C 50 and %T 35 %C 50 give the best characteristic. These monoliths have high permeability, adequate molecular recognition sites (represented with binding capacity value of over 20 mg/mL), and have over 80% flow through pores in their pore structure contribute to low flow resistance. The resulted monolithic columns have promising potential for dual mode liquid chromatography. MATE may contribute for anion-exchange while VBC may responsible for reversed-phase liquid chromatography.
Co-Authors Achmad Nurul Fauzie Adin Okta Triqadafi Agus Naba Akhmad Sabarudin Akhmad Sabarudin Aldi Dwi Putra Aprilia Nur Tasfiyati Arinto Yudi Ponco Wardoyo Dewi Anggraeni Didik Rahadi Santosa Didik Rahadi Santoso Eko Malis Elvina Dhiaul Iftitah Farida Wahyuni Fenny Irawati Hari Arief Dharmawan Hariyadi Soetedjo Lalu Sahrul Hudha Layli Amaliya Masruroh Masruroh Masruroh MASRUROH MUHAMMAD ULIN NUHA Nalle, Ferry Chrismiadi Nur Chabibah Rahmadwati Rizal Y Aji Saputra, Aditya Ilham Sigit Kurniawan Sri Sugiarti Sukma Wahyu Fitriani, Sukma Wahyu Triswantoro Putro Umi Salamah Unggul P. Juswono Unggul Punjung Juswono