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Rabies in Indonesia and The Development of Its:Diagnostic Techniques Adjid, R M A; Sarosa, A; Syafriati, T; ., Yuningsih
Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Rabies is a zoonotic disease which is crucial for public health, as it can infect human beings and causes death . Rabies has spread across the world including Indonesia . Control to erradicate rabies still faces many obstacles. Rabies research at Research Institute for Veterinary Science emphasizes on rabies erradication focussing on some aspects of diagnostic techniques. Studies on the spesimen preservation, the shipment to the laboratory and the collection of brain specimens by straw method as well as the use of cornea touch preparat as intra vitam specimens have been conducted. Rabies diagnostic techniques by NPLA or modified FAVN on multispot slides and their applications in the field have also been studied. This paper also describes the preparation of FITC conjugate. ELISA technique was developed for serological test but still need to be improved to reduce non specific reaction, while RREID was used for antigen detection. Rabies diagnostic was conducted by using RT-PCR but its application was very difficult to be done for intra vitam. Research on Croton tiglium and Pangium edule extracts as an alternative for the strychnine poison used for dog elimination in the field has also been conducted but further research is needed in the future. Key words: Rabies, zoonosis, research, diagnostic

Isolation of Pasteurella multocida from broiler chickens Poernomo, Sri; Sarosa, A
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 2 (1996)
Publisher : Indonesian Animal Sciences Society

Pasteurella multocida, the etiological agent of fowl cholera, was isolated from five, 32 days oldbroilerchickens in the late of 1992. The chickens were from a farm located in Bogor area, raised in cages and each flock consisted of 1,550 broilers . Therewere 230 birds, aging from 28-31 days old, died with clinical signs of lameness and difficulty in breathing. Serological test of the isolate revealed serotype Aof Carter classification . To prove Â its virulences, the isolate was then inoculated into 3 mice subcutaneously. The mice died less then 24 hours postinoculation and P. multocida can be reisolated . The sensitivity test to antibiotics and sulfa preparations showed that the isolate was sensitive to ampicillin, doxycyclin, erythromycin, gentamycin, sulfamethoxazol-trimethoprim and baytril, but resistance to tetracyclin, kanamycin and oxytetracyclin. This is the first report of P. multocida isolation in broiler chickens in Indonesia, and it is intended to add information on bacterial diseases in poultry in Indonesia. Â Keywords : Pasteurella multocida, isolation, broiler chickens, antibiotics

Salmonella Enteritidis infection in young broiler chickens from breeding farm: A case report Poernomo, Sri; Rumawasz, Indrawati; Sarosa, A
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 3 (1997)
Publisher : Indonesian Animal Sciences Society

old from a breeding farm not far from Bogor. Samples were examined pathologic anatomically (PA) and bacteriologically to isolate the causative agents . The sensitivity of the main causative agents isolated from the samples was tested with some drugs, while its pathogenicity was tested in 3 days old chickens intramuscularly, subcutaneously, intraperitoneally and orally, three chickens per inoculations . Exudative and caseous omphalitis, pericarditis, hepatitis, sirsacculitis, and coxofemoral and knee joints were observed in PA examinations, while on bacteriological examination the main cusative agent, ie. Salmonella Enteritidis was isolated successfully . Drug sensitivity test showed that the pathogen was sensitive to chloramphenicol, baytril, gentamisin, and sulphametoxazole-trimethoprim, and resistant to erythromycin, colistin, streptomycin and kanamycin . On the other hand, pathogenicity test of the isolate showed that all but two chickens which were inoculated orally, were died 24 hours post-inoculation . It was concluded that young broiler chickens of the farm were infected by Salmonella Enteritidis. Â Keywords : Salmonella Enteritidis infection, broiler chickens, breeding farm

The prevalence of Japanese-B-Encephalitis in different species in Indonesi I, Sendow; Bahri, S; Sarosa, A
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 1 (2000)
Publisher : Indonesian Animal Sciences Society

Japanese-B-Encephalitis (JE) is a zoonotic disease which is characterized by encephalitis, caused by JE virus. The situation of this disease has not been known in both animals and human in Indonesia. This paper reports serological finding using competitive - ELISA to evaluate 953 serum samples, comprised of chicken, ducks, cattle, goats, horses, dogs, pigs and human from different areas in Indonesia. The antibody against JE virus was detected in animals and human sera, with prevalence varied among species and location. Cattle showed the highest prevalence of reactor (51 %) while pigs, dogs and horses had the lowest reactor (11%,12% and ]4%). The highest prevalence of reactor in cattle was found in North Sumatera (86%) and the lowest was found in West Java (23%). In goat, the highest prevalence of reactor was found in West Kalimantan (59%) and the lowest was detected in South Sulawesi (14%). Antibody against JE virus was also detected in chicken with the highest prevalence in North Sumatera and West Kalimantan (44%) and the lowest was in South Sulawesi (36%). The highest percentage of reactor in pigs was detected in South Sulawesi (50%) and the lowest was detected in West Kalimantan (2%). In human, the highest prevalence of reactor was found in West Kalimantan (30%) and the lowest was fowld in Irian Jaya. This result provide more information for further research, therefor the JE cases in Indonesia and its social, economic and psychological impacts can be anticipated as earlyas possible. Â Key words: Japanese-B-Encephalitis, antibody, animals, human, ELISA

Isolation of Pasteurella multocida from broiler chickens Sri Poernomo; A Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 2 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Pasteurella multocida, the etiological agent of fowl cholera, was isolated from five, 32 days oldbroilerchickens in the late of 1992. The chickens were from a farm located in Bogor area, raised in cages and each flock consisted of 1,550 broilers . Therewere 230 birds, aging from 28-31 days old, died with clinical signs of lameness and difficulty in breathing. Serological test of the isolate revealed serotype Aof Carter classification . To prove its virulences, the isolate was then inoculated into 3 mice subcutaneously. The mice died less then 24 hours postinoculation and P. multocida can be reisolated . The sensitivity test to antibiotics and sulfa preparations showed that the isolate was sensitive to ampicillin, doxycyclin, erythromycin, gentamycin, sulfamethoxazol-trimethoprim and baytril, but resistance to tetracyclin, kanamycin and oxytetracyclin. This is the first report of P. multocida isolation in broiler chickens in Indonesia, and it is intended to add information on bacterial diseases in poultry in Indonesia. Keywords : Pasteurella multocida, isolation, broiler chickens, antibiotics

The prevalence of Japanese-B-Encephalitis in different species in Indonesi Sendow I; S Bahri; A Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 1 (2000): MARCH 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Japanese-B-Encephalitis (JE) is a zoonotic disease which is characterized by encephalitis, caused by JE virus. The situation of this disease has not been known in both animals and human in Indonesia. This paper reports serological finding using competitive - ELISA to evaluate 953 serum samples, comprised of chicken, ducks, cattle, goats, horses, dogs, pigs and human from different areas in Indonesia. The antibody against JE virus was detected in animals and human sera, with prevalence varied among species and location. Cattle showed the highest prevalence of reactor (51 %) while pigs, dogs and horses had the lowest reactor (11%,12% and ]4%). The highest prevalence of reactor in cattle was found in North Sumatera (86%) and the lowest was found in West Java (23%). In goat, the highest prevalence of reactor was found in West Kalimantan (59%) and the lowest was detected in South Sulawesi (14%). Antibody against JE virus was also detected in chicken with the highest prevalence in North Sumatera and West Kalimantan (44%) and the lowest was in South Sulawesi (36%). The highest percentage of reactor in pigs was detected in South Sulawesi (50%) and the lowest was detected in West Kalimantan (2%). In human, the highest prevalence of reactor was found in West Kalimantan (30%) and the lowest was fowld in Irian Jaya. This result provide more information for further research, therefor the JE cases in Indonesia and its social, economic and psychological impacts can be anticipated as earlyas possible. Key words: Japanese-B-Encephalitis, antibody, animals, human, ELISA

Salmonella Enteritidis infection in young broiler chickens from breeding farm: A case report Sri Poernomo; Indrawati Rumawasz; A Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

old from a breeding farm not far from Bogor. Samples were examined pathologic anatomically (PA) and bacteriologically to isolate the causative agents . The sensitivity of the main causative agents isolated from the samples was tested with some drugs, while its pathogenicity was tested in 3 days old chickens intramuscularly, subcutaneously, intraperitoneally and orally, three chickens per inoculations . Exudative and caseous omphalitis, pericarditis, hepatitis, sirsacculitis, and coxofemoral and knee joints were observed in PA examinations, while on bacteriological examination the main cusative agent, ie. Salmonella Enteritidis was isolated successfully . Drug sensitivity test showed that the pathogen was sensitive to chloramphenicol, baytril, gentamisin, and sulphametoxazole-trimethoprim, and resistant to erythromycin, colistin, streptomycin and kanamycin . On the other hand, pathogenicity test of the isolate showed that all but two chickens which were inoculated orally, were died 24 hours post-inoculation . It was concluded that young broiler chickens of the farm were infected by Salmonella Enteritidis. Keywords : Salmonella Enteritidis infection, broiler chickens, breeding farm

Rabies in Indonesia and The Development of Its:Diagnostic Techniques R M A Adjid; A Sarosa; T Syafriati; Yuningsih .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development

Rabies is a zoonotic disease which is crucial for public health, as it can infect human beings and causes death . Rabies has spread across the world including Indonesia . Control to erradicate rabies still faces many obstacles. Rabies research at Research Institute for Veterinary Science emphasizes on rabies erradication focussing on some aspects of diagnostic techniques. Studies on the spesimen preservation, the shipment to the laboratory and the collection of brain specimens by straw method as well as the use of cornea touch preparat as intra vitam specimens have been conducted. Rabies diagnostic techniques by NPLA or modified FAVN on multispot slides and their applications in the field have also been studied. This paper also describes the preparation of FITC conjugate. ELISA technique was developed for serological test but still need to be improved to reduce non specific reaction, while RREID was used for antigen detection. Rabies diagnostic was conducted by using RT-PCR but its application was very difficult to be done for intra vitam. Research on Croton tiglium and Pangium edule extracts as an alternative for the strychnine poison used for dog elimination in the field has also been conducted but further research is needed in the future. Key words: Rabies, zoonosis, research, diagnostic

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