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All Journal Jurnal Akuakultur Indonesia Journal of Aquaculture Science Jurnal Puruhita Journal of Fish Health
Ade Dwi Sasanti
Sriwijaya University
Agriculture, Biological Sciences & Forestry Social Sciences

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The Effect of Immersion Duration in Thyroxine Hormone on Growth of Snakehead Fish Larvae (Channa striata) Muslim, Muslim; Sasanti, Ade Dwi; Apriana, Apriana
Journal of Aquaculture Science Vol 4, No 1 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.048 KB) | DOI: 10.31093/joas.v4i1.63

Abstract

The aim of this research was to determine the best immertion duration to increase growth rate of snakehead fish larva. The research was conducted in Fish Breeding unit Batanghari Sembilan Indralaya, Kabupaten Ogan Ilir. This research used a Completely Randomized Design (CRD), with five treatments (0, 12, 24, 36, and 48 hours of immertion), with three replications. The parameters observed were growth, survival rate and water quality. The results of  this research show that the highest growth rate found in the 24 hours of treatment  with the average value of 0.17 g of weight and increase in length of 1.90 cm. The best survival contained in treatment of immersion for 36 hours with the average value of 71.67%. Key words: snakehead larvae, thyroxine, growth, immertion
Pemanfaatan Limbah Urin Sapi sebagai Bahan Dasar Pembuatan Bioinsektisida Berbasis Bacillus thuringiensis di Desa Sejaro Sakti Kecamatan Indralaya Kabupaten Ogan Ilir Pujiastuti, Yulia; Gunawan, Bambang; Sulistyani, Dwi Probowati; Sandi, Sofia; Sasanti, Ade Dwi
Jurnal Puruhita Vol 3 No 1 (2021): February 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/puruhita.v3i1.53051

Abstract

Desa Sejaro Sakti terletak di Kecamatan Indralaya ibukota kabupaten Ogan Ilir Sumatera Selatan. Potensi peternakan sangat besar dengan jumlah ternak sapi yang banyak dan ladang penggembalaan yang cukup luas. Salah satu jenis limbah yang dihasilkan berupa urin sapi. Limbah cair tersebut belum dimanfaatkan dengan baik. Sebagai bahan yang mengandung unsur karbon dan nitrogen (C dan N), urin sapi dapat dimanfaatkan sebagai media pembuatan bioinsektisida berbasis bakteri entomopatogen, Bacillus thuringiensis. Dalam aplikasinya, urin sapi diperkaya dengan penambahan 5% molase. Hasil penelitian sebelumnya menghasilkan data tentang kandungan spora dan protein bakteri B. thuringiensis yang dapat mematikan serangga hama. Pada umumnya bioinsektisida berbasis B. thuringiensis digunakan untuk mengendalikan serangga hama namun aman bagi musuh alami. Tujuan kegiatan pengabdian ini untuk memperkenalkan cara membuat bioinsektisida berbasis B. thuringiensis dengan bahan dasar limbah urin sapi. Sasaran kegiatan ini adalah kelompok tani di Desa Sejaro Sakti. Kegiatan diadakan mulai buan September-November 2019. Metode kegiatan berupa penyuluhan dan sosialisasi tentang potensi desa, arti penting dan manfaat limbah urin sapi, serta demonstrasi pembuatan bioinsektisida. Hasil kegiatan pengabdian menunjukkan bahwa petani sangat antusias mendapatkan pengetahuan dan ketrampilan tentang pembuatan bioinsektisida berbasis B. thuringiensis. Sebagian besar petani peserta kegiatan paham tentang cara pembuatan bioinsektisida. Mereka menyatakan bahwa pembuatan bioinsektisida tersebut merupakan hal baru dan mereka berminat untuk mempraktekkannya. Telah disadari bahwa selama ini pengendalian serangga hama biasanya dilakukan dengan menggunakan insektisida kimia. Oleh karena itu, untuk mengurangi biayaa pengendalian, mereka akan menerapkannya dalam usaha pengendalian hama di lahan pertanian. Hasil uji coba yang dilakukan di lahan terbatas menunjukkan bahwa lahan yang menggunakan bioinsektisida B. thuringiensis menunjukkan pertumbuhan tanaman yang lebih baik dibandingkan dengan tanpa perlakuan.
Isolation of probiotics bacterium from coral reef for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae Sasanti, Ade Dwi; Widanarni, .; Sukenda, .
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.702 KB) | DOI: 10.19027/jai.9.168-177

Abstract

Pathogenic Vibrio, especially luminous Vibrio harveyi, could cause mass mortality in tiger shrimp culture. One of the technique to work against luminous Vibrio is, using probiotic bacteria to inhibit the luminous Vibrio growth. This study was carried out to obtain bacteria isolates from coral reef which potentially inhibit V. harveyi growth. A total of 110 isolates were isolated from Acropora sp, Merulina sp, Hystrix sp., Poecillophora sp, Porites sp and Haliophora sp., and have probiotic activity against V. harveyi in in vitro and in vivo test.Of the total 110 isolates, 54 isolates show the inhibiting zone. Two isolates(8A and 1C)were not pathogenic and have the most effective activity in inhibiting growth of V. harveyi and significantly reduced larval mortality in in vitro and in vivo test. Treatment using probiotics candidate have significant different survival rate (83.33%) compared with positive control (61.67%). The growth rate of lenght of larvae treatment with isolate of 8A (5.25%) and 1C (5.06%) show the significant different compared with positive control (3.54%). The growth rate of weight of larvae treatment withisolate of 8A (17.51%) and 1C (17.61%) show significant different compared with negative (15.27%) and positive control (14.69%).Key word: coral reef, probiotic, tiger shrimp, vibriosis, V. harveyi. ABSTRAKVibrio patogen, khususnya Vibrio harveyi berpendar, dapat menyebabkan kematian massal pada budidaya udang windu. Salah satu alternatif untuk menghambat Vibrio harveyi berpendar adalah dengan menggunakan bakteri probiotik yang dapat menekan pertumbuhan Vibrio tersebut. Penelitian ini bertujuan untuk mendapatkan isolat bakteri dari terumbu karang yang potensial menghambat pertumbuhan V. harveyi. Total 110 isolat diisolasi dari Acropora sp, Merulina sp., Hystrix sp., Poecillophora sp., Porites sp. dan Haliophora sp, dilakukan penapisan untuk melihat aktivitas kemampuannya melawan V. harveyi MR 5339 RfR dalam uji in vitro dan uji in vivo. Sebanyak 54 isolat teridentifikasi mampu menghasilkan zona hambat. Dua isolat (8A dan 1C) terbukti tidak bersifat patogen dan memiliki aktivitas probiotik melawan V. harveyi MR 5339 RfR pada uji in vitro dan in vivo. Tingkat kelangsungan hidup larva pada perlakuan yang diberi kandidat probiotik dari kedua isolat tersebut adalah 83,33%, dan menunjukkan hasil yang berbeda nyata dengan kontrol positif (61,67%). Laju pertumbuhan panjang larva yang diberi isolat 8A (5,25%) dan 1C (5,06%)menunjukkan hasil yang berbeda nyatajika dibandingkan dengan kontrolpositif (3,54%). Laju pertumbuhan bobot yang diberi isolat 8A (17,51%) dan 1C (17,61%) menunjukkan hasil yang berbeda nyata terhadap kontrol negative (15,27%) dan kontrol positif (14,69%).Kata kunci: probiotik, terumbu karang,udang windu, V. harveyi, vibriosis.
Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae Sasanti, Ade Dwi; ., Widanarni; ., Sukenda
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.248 KB) | DOI: 10.19027/jai.10.174-182

Abstract

ABSTRACTThis study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reefABSTRAKPenelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang
Impact of Probiotic Swamp Bacteria on the Prevention of Aeromonas hydrophila Infection in Striped Snakehead (Channa striata) Wijayanti, Marini; Sasanti, Ade Dwi; Jubaedah, Dade; Citra, Nyayu
Journal of Fish Health Vol. 5 No. 2 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i2.6631

Abstract

Probiotics are microbes that can modify the bacterial structure in fish digestive systems. Probiotics are used as feed additives and can improve the host's health. As a result, it can help to prevent Aeromonas infection. This study aims to determine the effectiveness of bacteria from the swamp (Bacillus sp. and Streptomyces sp.) on feed in preventing Aeromonas hydrophila infection in striped snakeheads. The study used P0 (control), P1 (Bacillus sp.), P2 (Streptomyces sp.), P3 (Bacillus sp. and Streptomyces sp. combination), each bacterium 10 ml kg-1 feed. Total of bacteria, prevalence, fish growth, survival rate, and water quality are among the variables assessed. The fish survival, fish growth, and feed efficiency in Channa treated with a combination of Bacillus and Streptomyces (P3) were significantly highest among other, resulting of best prevalence 2.38%.
Co-Authors Agus Supriadi Apriana Apriana Bambang Gunawan Citra, Nyayu Dade Jubaedah Marini Wijayanti Muslim Muslim Radyanti Darsi Sofia Sandi Sukenda . Sulistyani, Dwi Probowati WIDANARNI WIDANARNI YULIA PUJIASTUTI