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All Journal JSFK (Jurnal Sains Farmasi & Klinis) B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Farmasi Higea Journal of Multidisciplinary Applied Natural Science Journal of Fibers and Polymer Composites Biodiversitas Journal of Biological Diversity
Dian Handayani
Fakultas Farmasi Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Uncovering The Pharmacological Mechanism of Ficus elastica as Anti-hyperlipidemia Candidate: LC-HRMS, Network Pharmacology, In vitro and In vivo Studies Gita Susanti; Yufri Aldi; Dian Handayani; Friardi Ismed; Arif Setiawansyah
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.249

Abstract

Hyperlipidemia is a major risk factor for cardiovascular diseases. While conventional treatments exist, there is a growing interest in natural remedies with fewer side effects. Ficus elastica has promising medicinal properties, yet its potential as an anti-hyperlipidemic agent remains unexplored. This study aimed to investigate the anti-hyperlipidemic effects of F. elastica using an integrated approach of LC-HRMS-based chemical bioinformatics and in vitro/in vivo experimental validation. The anti-hyperlipidemic potential of F. elastica and its mechanism of action were screened using integrative computational network pharmacology followed by in vitro HMG-CoA reductase inhibition and in vivo lipid-lowering activity in a hyperlipidemia rat model. Network pharmacology analysis identified STAT3, HSP90AA1, and TLR4 as potential core targets involved in lipid and atherosclerosis-related KEGG pathways. Molecular docking simulations revealed high-affinity interactions between F. elastica compounds and the identified targets, notably compound 41 and compound 61. In vitro assay demonstrated that ethanolic extract of F. elastica inhibited HMG-CoA reductase with an IC50 of 297.73 µg/mL. In vivo experiment using a hyperlipidemic rat model showed significant reductions in total cholesterol, triglycerides, and increased HDL levels. The reduction of triglycerides and elevation of HDL level after F. elastica ethanolic extract supplementation is similar to the effect from supplementation of simvastatin. These findings suggest that F. elastica ethanolic extract possesses notable anti-hyperlipidemic properties, likely mediated through multiple molecular targets and pathways. The study highlights the potential of F. elastica ethanolic extract as a promising candidate for anti-hyperlipidemic therapy and underscores the efficacy of integrating computational and experimental approaches in natural product research.
Modified PVA Film from Methanol-Soluble Phenolic Extracts of Spatholobus littoralis Hask as Active Pharmaceutical Packaging Kadriadi Kadriadi; Hairul Abral; Melbi Mahardika; Ilhamdi Ilhamdi; Akmal Akmal; Dian Handayani; Yulianis Yulianis; Mohamad Haafiz Mohamad Kassim; Jeri Ariksa
Journal of Fibers and Polymer Composites Vol. 5 No. 1 (2026): Journal of Fibers and Polymer Composites
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jfpc.v5i1.567

Abstract

The development of active pharmaceutical packaging based on biodegradable materials is an important strategy to reduce dependence on single-use plastics and their environmental impact. Polyvinyl alcohol (PVA) is a potential biodegradable polymer, but it has limitations in terms of exposure to ultraviolet (UV) radiation and microbial contamination. This study aims to develop a modified PVA film with methanol-soluble phenolic extract of Spatholobus littoralis Hask as active pharmaceutical packaging with UV protection, antioxidant, and antibacterial functions. The phenolic extract was obtained through a maceration method using methanol as a solvent, while the PVA film was fabricated using the solution casting technique. The PVA film was modified with varying concentrations of phenolic extract of 0, 1.25, 2.5, and 5wt% (PPE0, PPE1.25, PPE2.5, and PPE5), then evaluated for its UV protection properties, antioxidant activity, and antibacterial activity. The results showed that the addition of S. littoralis phenolic extract was able to increase the ability of PVA films to block UV radiation completely (100%) in the 200–400 nm wavelength range. Antioxidant activity testing using the DPPH method showed an increase in free radical scavenging ability as the concentration of phenolic extract increased. In addition, the modified PVA film showed significant antibacterial activity against Staphylococcus aureus and Escherichia coli. These findings indicate that S. littoralis Hask phenolic extract has potential as a natural bioactive agent in the development of environmentally friendly and multifunctional active pharmaceutical packaging, with dual protection capabilities against UV degradation and microbial contamination. This research makes an important contribution to the development of sustainable pharmaceutical packaging materials based on renewable natural resources.
Fungal isolates from marine sponge Chelonaplysilla sp.: Diversity, antimicrobial and cytotoxic activities Dian Handayani; MUH. ADE ARTASASTA; NILDA SAFIRNA; DIANA FITRI AYUNI; TRINA EKAWATI TALLEI; TRIANA HERTIANI
Biodiversitas Journal of Biological Diversity Vol. 21 No. 5 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210523

Abstract

Abstract. Handayani D, Artasasta MA, Safira N, Ayuni DF, Tallei TE, Hertiani T. 2020. Fungal isolates from marine sponge Chelonaplysilla sp.: Diversity, antimicrobial and cytotoxic activities. Biodiversitas 21: 1954-1960. The purpose of this research was to study the diversity of fungi associated with marine sponges Chelonaplysilla sp. and their bioactivities. Fungal isolation was carried out by the multilevel dilution method in Saboraud Dextrose Agar (SDA). Twelve fungal isolates were successfully purified, then cultivated using rice for 4-6 weeks at room temperature and subsequently extracted using ethyl acetate. Antimicrobial activities of the fungal extracts were tested against Staphylococcus aureus, Escherichia coli, and Candida albicans by using the agar diffusion method. The extracts of isolates Ch05 and Ch12 showed a significant antagonistic effect against S. aureus and E. coli with the diameter that ranged from 15 to 17 mm. Using the brine shrimp lethality test (BSLT), six fungal extracts revealed cytotoxic activity with LC50 <100 µg/mL. Isolate Ch10 was the most potential fungus with the strong cytotoxic activity of LC50 of 0.90 µg/mL. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was conducted also for six potential fungal extracts against breast cancer cell (T47D). The isolate Ch05 showed moderate cytotoxic activity with IC50 of 83.69 µg/mL. The molecular identification was carried out for potential fungi using the ITS marker. The results showed that Ch02 was Aspergillus oryzae, Ch05 was Phomompsis sp., Ch06 was Penicillium simplicissimum, Ch10 was B. bassiana and Ch12 was Aspergillus mellinus. This study concluded that fungal isolates from marine sponge Chelonaplysilla sp. can be explored further for new sources of antimicrobial and anticancer compounds.
Isolation and evaluation of the antimicrobial activity of endophytic fungi from Orthosiphon aristatus HERLAND SATRIAWAN; NIKY AFRILIA ZAIMI; ARIED ERIADI; MAI EFDI; TRINA EKAWATI TALLEI; REGINA ANDAYANI; DIAN HANDAYANI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 2 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260245

Abstract

Abstract. Satriawan H, Zaimi NA, Eriadi A, Efdi M, Tallei TE, Andayani R, Handayani D. 2025. Isolation and evaluation of the antimicrobial activity of endophytic fungi from Orthosiphon aristatus. Biodiversitas 26: 963-970. Orthosiphon aristatus, known as cat's whiskers or Javanese tea in Indonesia, has been used in traditional medicine to enhance urination, treat kidney inflammation, mitigate kidney stones, regulate diabetes, address albuminuria and syphilis, alleviate rheumatism, reduce blood glucose levels, and function as an antibacterial agent. The purpose of this study was to investigate the endophytic fungal resources of Orthosiphon aristatus and evaluate its potential as a source of bioactive compounds, particularly against antibiotic-resistant pathogens like Methicillin-resistant Staphylococcus aureus (MRSA). Eleven fungal isolates were obtained from the plant’s leaves, stems, and roots. The ethyl acetate extract of isolates was subjected to comprehensive antimicrobial screening at a concentration of 5%. Three isolates-OAA1, OAA3, and OAD1-exhibited considerable antibacterial activity, with OAD1 displaying the most significant efficacy, as evidenced by a 12.57 ± 1.09 mm inhibition zone against MRSA. Nevertheless, none of the extracts could suppress the proliferation of Candida albicans. Subsequent molecular identification verified that OAD1 was Aspergillus niger. The phytochemical analysis revealed that the triterpenoid and flavonoid groups exhibited a positive reaction, whereas the alkaloid and phenol compound groups exhibited a negative response. The findings highlight the potential of endophytic fungi from O. aristatus as promising sources of novel bioactive compounds, especially for addressing antibiotic-resistant pathogens like MRSA. The identification of A. niger as a powerful antibacterial-producing organism presents promising prospects for the creation of novel pharmaceuticals, particularly regarding multidrug-resistant infections. This study emphasizes the pressing necessity for additional research to identify and characterize the specific compounds that confer antibacterial activity and to investigate their extensive therapeutic applications.
Co-Authors Abral, Hairul Akmal Akmal Akmal Djamaan Anzharni Fajrina Aried Eriadi Arif Setiawansyah Corry Handayani Deddi Prima Putra DIANA FITRI AYUNI Elza Masriza Indah Pratiwi Friardi Ismed Gita Susanti Harrizul Rivai HERLAND SATRIAWAN Ilhamdi Ilhamdi Jeri Ariksa Kadriadi Kadriadi Krisyanella Krisyanella Lendra Yunance Mai Efdi Mega Yulia Melbi Mahardika Meliyana Meliyana Mohamad Haafiz Mohamad Kassim MUH. ADE ARTASASTA Nicole J. de. Voogd NIKY AFRILIA ZAIMI NILDA SAFIRNA Nisya Ogiana Rafky Putra REGINA ANDAYANI Rendowaty, Agnes TRIANA HERTIANI TRINA EKAWATI TALLEI TRINA EKAWATI TALLEI Wiza Leila Puspita Sari Yahdian Rasyadi Yohanes Allen Yufri Aldi Yulianis Yulianis