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Standarisasi Simplisia Daun Kenikir (Cosmos caudatus Kunth) Dari Tiga Daerah Berbeda Miftahul Jannah; Sumi Wijaya; Henry Kurnia Setiawan
Jurnal Farmasi Sains dan Terapan Vol 8, No 1 (2021): Februari
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v8i1.3087

Kenikir dipercaya sebagai obat penurun suhu tubuh, memperbaiki sirkulasi darah, mengobati diabetes, sebagai anti-aging dan menjaga kekuatan tulang. Saat ini telah banyak produk serbuk daun kenikir dalam bentuk kapsul di pasaran, tetapi belum ada data standarisasi dari simplisia daun kenikir, sehingga penelitian ini dilakukan bertujuan untuk mendapatkan profil standarisasi spesifik dan non-spesifik dari simplisia daun kenikir. Parameter spesifik terdiri dari identitas simplisia, organoleptis, kadar sari laut air, kadar sari larut etanol, penetapan profil kromatografi dengan menggunakan KLT, penetapan profil spektrum dengan menggunakan spektrofotometer infrared dan UV-Vis serta penetapan kadar senyawa metabolit sekunder. Parameter non spesifik meliputi susut pengeringan, kadar abu total, kadar abu tak larut asam, kadar abu larut air, pH, dan persen bahan asing. Data yang diperoleh merupakan data deskriptif yang diperoleh dari 3 lokasi berbeda. Hasil pengamatan mikroskop daun segar kenikir menunjukkan tipe berkas pembuluhnya adalah kolateral terbuka, jaringan bunga karang atau palisade, dengan tipe stomata anomositik, rambut penutup non-glandullar, kristal Ca-oksalat bentuk pisma dan tipe daun dorsiventral. Hasil kadar sari larut etanol > 23,3%, kadar sari larut air > 13,15%. Hasil skrining fitokimia menunjukkan adanya senyawa flavonoid, saponin, polifenol, steroid dan triterpenoid. Hasil pengamatan profil kromatogram secara KLT dengan fase diam silika gel F254 dan fase gerak yang terpilih adalah n-heksan : etil asetat (7:3). Hasil penetapan kadar flavonoid total > 1,30%, fenol > 2,18%. Nilai susut pengeringan ≤13,5%, sedangkan hasil kadar abu total ≤6,0% dengan kadar abu larut air ≤5,0% dan kadar abu tak larut asam ≤2,0%, pH simpisia untuk pelarut air yaitu 6- 7 dan pada pelarut etanol 4 – 6.

Identifikasi Deksametason Dan Paracetamol Dalam Jamu Asam Urat Dan Rematik Secara Kromatografi Lapis Tipis-Densitometri Mochammad Fauzan Oktaviano; Diana; Henry Kurnia Setiawan
SNHRP Vol. 5 (2023): Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Ke 5 Tahun 2023
Publisher : LPPM Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Sediaan Jamu di Indonesia dipersyaratkan tidak boleh mengandung Bahan Kimia Obat (BKO). Jamu diperdagangkan secara bebas sehingga penambahan BKO kedalamnya dikhawatirkan dapat menimbulkan efek yang tidak diinginkan karena tidak adanya kontrol akan dosis dan penggunaannya. Hasil pengujian Badan Pengawas Obat dan Makanan menunjukkan masih maraknya penambahan BKO ke dalam sediaan jamu. Validasi Metode Analisa untuk identifikasi BKO Deksametason dan Paracetamol pada sediaan jamu asam urat dan rematik menggunakan Kromatografi Lapis Tipis (KLT) - Densitometri dilakukan pada penelitian ini. Pengujian dilanjutkan dengan mengidentifikasi keberadaan kedua BKO tersebut dalam 10 sampel jamu yang ada di Indonesia. Metode analisa dilakukan menggunakan plat silika gel 60 F 254 dan fase gerak kloroform : etil asetat (1:4) pada panjang gelombang pengamatan 245 nm. Hasil validasi menunjukkan metode spesifik, dimana Paracetamol dan Deksametason memiliki Rf berturutturut adalah 0,58 dan 0,68 dengan resolusi 2,0. Batas deteksi (LOD) metode adalah 10,3 µg/mL untuk deksametason dan 7,1 µg/mL untuk paracetamol. Dari pengujian 10 sampel sediaan, 5 dari antaranya diketahui positif mengandung BKO deksametason.

Fraksinasi dan Identifikasi Senyawa Antioksidan pada Ekstrak Etanol Daun Sirsak (Annona muricata L.) secara Kromatografi Kolom Sari, Diyan Maya; Wijaya, Sumi; Setiawan, Henry Kurnia
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 2 No. 2 (2015)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v2i2.725

Annona muricata L. merupakan salah satu tanaman dari familia Annonaceae yang mengandung antioksidan alami yang dapat digunakan untuk pengobatan berbagai jenis penyakit. Berdasarkan kemampuannya yang memiliki daya antioksidan tersebut, maka dilakukan pemisahan senyawa antioksidan dan uji untuk mem-bandingkan aktivitas daya antioksidan dari ekstrak etanol dengan fraksinya. Pemisahan senyawa antioksidan dengan menggunakan kromatografi kolom dan pengujian aktivitas antioksidan dengan metode DPPH (1,1-diphenyl-2-picrylhidrazyl). Berdasarkan hasil data KLT, spektrum UV-Vis dan spektroskopi IR dapat disimpul-kan bahwa senywa antioksidan mengandung flavonoid. Hasil pengujian aktivitas antioksidan menunjukkan bahwa ekstrak etanol daun sirsak memiliki aktivitas antioksidan dengan nilai 0,25 ± 0,037 mg/mL, sedang-kan hasil dari fraksi menunjukkan bahwa aktivitas antioksidannya lebih besar dengan nilai 0,14 ± 0,011 mg/mL. Golongan metabolit sekunder dalam fraksi etanol daun sirsak yang dapat berfungsi sebagai senyawa antioksidan adalah flavonoid dan fraksi etanol tersebut memiliki aktivitas antioksidan yang lebih besar di-bandingkan dengan aktivitas antioksidan ekstrak etanol. Kata kunci: Annona muricata L., antioksidan, kromatografi kolom, DPPH.

Standarisasi dari Daun Kirinyuh (Chromolaena odorata) dan Simplisia Kering dari Tiga Daerah yang Berbeda Ance, Paulina Erlianda; Wijaya, Sumi; Setiawan, Henry Kurnia
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 5 No. 2 (2018)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v5i2.2140

Siam wead (Chromolaena odorata) is used traditionally for treatment lesoin, amenorrhea, antidiabetic and antimicrobial. Based on previous research, there is no reference about the standardization Siam Weed leaves dried powder. This study aims to determine macroscopic and microscopic of siam weed leaves fresh plant obtained from, Surabaya, to determine the spesific and non spesific quality profile of dried powder of siam weed leaves dried powder obtained from different areas (Bogor, Malang and Surabaya). The spesific parameter that used were identity, organoleptic, microscopic, determination of soluble extract, phytochemical screening, determination chromatogram profile using Thin Layer Chromatography, determination spectrum profile using spektrophotometer UV-Vis and Infrared, determination of alkaloid, phenol and flavonoid content. Non spesific parameters that used were the determination of total ash value, water soluble ash value, acid insoluble ash value, drying losses and pH. Based on result of the study, it was concluded that siam weed fresh plant leaves have elliptico-oblongus shape, acutus shape for the upper and lower base, green colored for the top and bottom surfaces, adge shape for the wavy , 6.4-11.8 (L), 3.3-5.9 (W), wavely leaf rib, fluffy leaf texture, and the position of facing a single, microscopically have the fragments as same as the dried powder fragments, ethanol soluble concentration ≥ 12%, water soluble concentration ≥ 20%, solvent can be used for chromatogram profile in TLC is chloroform : ethyl acetate (15:1), spectral profile with spectrophotometer UV-Vis have wavelength 533.5 nm, 500 nm, 534.5 nm, 501.5 nm, 284.5 nm, 289 nm and 292 nm, spectral profile with IR spectrophotometers have wavenumber 3266.28 cm-1-3282.21 cm-1, 1416.01 cm-1, 1514.53 cm-1, 1514.93 cm-1, 1245.35 cm-1, 1249.52 cm-1 dan 1251.27 cm-1, flavonoid content ≥ 0.4%, phenol content ≥ 1.0% and alkaloid content ≥ 1.0%, total ash content ≤ 14%, water ash soluble ≤ 5%, acid unsoluble ash ≤ 10%, drying losses ≤ 11% and range pH 5-7.

Standarisasi Spesifik dan Non Spesifik dari Ekstrak Etanol Daun Beluntas (Pluchea indica L. Less.) Wijaya, Sumi; Setiawan, Henry Kurnia; Ano, Lucyanna Ayu Lestari
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 4 No. 1 (2017)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v4i1.2178

Indian fleabane (Pluchea indica (L.) Less.), on of the species in Asteraceae family, is widely used for traditional medicine. A standardization process is needed as an effort to improve the quality and safety of products, so that it is expected to increase the trust in drugs derived from natural ingredients. This study aims to determine the morphology and anatomy of Indian fleabane leaves and to determine the standardization value of the ethanol extract of Indian fleabane leaves. Dried powder of Indian fleabane leaves are obtained from three different regions (Bogor, Malang and Surabaya). The extract was obtained by maceration method using 96% ethanol as a solvent. Specific standardization includes identity, macroscopic, determination of soluble extract, phytochemical screening, TLC (Thin Layer Chromatography) pattern determination, determination of infrared spectrum profile, determination of UV-Vis spectrum profile, and determination of flavonoid, phenol and alkaloid total content. Nonspecific standardization includes determination of total ash content, water soluble ash content, acid insoluble ash content, drying losses, specific gravity and pH. Organoleptic observations showed the ethanol extract of Indian fleabane leaves has blackish brown and aromatic odour. The mobile phase being used for TLC profile chromatogram was n-hexane: ethyl acetate (7: 3). Based on the results, the screening results of Indian fleabane contain alkaloids, flavonoids, polyphenols, tannins, saponins, steroids and triterpenoids. The Infra red spectrophotometer analysis showed the range of wave numbers of 2924 - 2925, 1515 - 1516, 1259 - 1260, 1159 - 1162, 1112 - 1115, 1046 - 1048, 811 - 812, 505 - 506 and 452 - 454 cm- 1. The standardization value of Indian fleabane ethanol extract showed ethanol soluble extract> 65%, water soluble extract> 49%, water content

Standarisasi Simplisia Kering Daun Beluntas (Pluchea indica L.) dari Tiga Daerah Berbeda Talia, Sela; Wijaya, Sumi; Setiawan, Henry Kurnia
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 4 No. 2 (2017)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v4i2.2181

Indian Fleabane (Pluchea indica L.) are commonly used as hedgerows and traditionally the leaves are used as fresh salad or medicines to eliminate body odor, febrifuge, cough medicine, and antidiarrheal drugs. Along with the increasing technology of natural materials and the tendency of people to use products derived from natural materials, especially medicinal plants, a reference which contains the requirements for the quality of natural ingredients that are suitable for use as medicinal ingredients is needed. This research aims to determine the profile of specific and non specific standardization of dried powder of indian fleabane leaves. Standardization of indian fleabane leaves covers specific parameters and non specific parameters, characterization of microscopic characteristics of indian fleabane (Pluchea indica L.) leaves, characterization of the content of secondary metabolites using thin layer chromatography (TLC), determination of spectrum profiles using infrared spectrophotometer (IR), and determination of the levels of secondary metabolites by spectrofotometry. The data obtained is descriptive data that reflect the acquisition of data from 3 different locations. Based on microscopic observations indian fleabane leaf dried powder has vascular bundles with spiral thickening, anomocytic stomata, and multicellular trichomes. Ethanol solubility of dried powder indian fleabane is ≥5% while water solubility in water is ≥26%. The results of phytochemical screening showed positive results on the observations of alkaloids, flavonoids, phenols, steroids and terpenes. Total ash content is

Standarisasi Spesifik dan Non Spesifik dari Ekstrak Etanol Daun dandang Gendis (Clinacanthus nutans) Wijaya, Sumi; Setiawan, Henry Kurnia; Purnama, Veronica Bella
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 6 No. 2 (2019)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v6i2.2224

Standardization of natural materials is needed as an effort to improve the quality and safety of products that are expected to further increase trust in medicines derived from natural ingredients. Snake grass leaf has several pharmacological activities including antioxidants, anticancer, anti-inflammatory, analgesic, enhancing the immune system, antibacterial, antivenom, even there are also uses in the cosmetics field. In this study macroscopic and microscopic observation of Snake grass leaves and determination of specific and non-specific standardization on ethanol extracts of Snake grass leaves have been done. The parameters tested on Snake grass leaf extract include the identity of the extract, organoleptic, ethanol soluble extract, water soluble extract, phytochemical screening, chromatogram profile using TLC, spectrum profile using UV-Vis spectrophotometer, spectrum profile using IR (infrared)spectrophotometer, determination of secondary metabolite content, total ash content, water soluble ash content, acid insoluble ash content, water content, pH, and specific gravity. The results showed the characteristics of ethanol extract of Snake grass leaf in the form of green-black extract, aromatic distinctive odor; ethanol soluble extract content> 54%; water soluble extract content> 37%; phytochemical screening results showed the presence of alkaloid compounds, flavonoids, polyphenols, saponins, steroids and terpenoids; the results of IR spectrophotometer analysis show spectrum profiles in the range wave of numbers 3325-3351 cm-1, 2924-2919 cm-1, 1622 -1633 cm-1, 1341-1345 cm-1 and 1020-1047 cm-1; total phenol levels> 0.16%; total flavonoid levels> 0.11%; total alkaloid levels> 0.03%; water content

Fraksinasi dan Identifikasi SenyawaAntioksidan pada Eksb-ak Etanol buah Goji beny(Lycium barbarum linn.) secaraKromatografi Kolom Albrian, Diga; Wijaya, Sumi; Setiawan, Henry Kurnia
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 1 No. 1 (2013)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v1i1.2346

Goji berry fruit (Lycium barbarum), familia Solanac'eae, is a red berry fruit. The chemical compounds contained in this fruit are polysaccharides, zeaxanthin and beta-carotene. Activity of the chemical compounds is still considered as a family of antioxidant compounds, which are used as a medicine to cure the diseases caused by the presence of free radicals such a~ atherosclerosis, cancer, liver cirrhosis, hypertension and diabetes. The research will be carried on fractionation and identification of the ethanol extract of Goji berry fruit to obtain compounds that have antioxidant power using chromatographic column method and DPPH method. The isolated compounds will be identified using methods phytochemica/s screening, TLC, UV-Vis spectrophotometer and infrared spectrophotometer. The result showed that secondary metabolites of Goji berry fruit which are potential as antioxidant compound were flavonoid (flavono/s or flavones). The isolated compound had higher antioxidant activity compare to the ethanol extract of Goji berry fruit Flavonoids are the major compounds that have antioxidant capacity. Whereas, the ethanol fraction of Goji berry fruits has the best antioxidant activity than the ethanol extract Keywords: Antioxidant, Goji berry, DPPH and chromatographic column

Validasi Metode Identifikasi Sildenafil Sitrat, Tadalafil dan Fenilbutazon dalam Jamu Obat Kuat Secara Kromatografi Lapis Tipis – Densitometri Setiawan, Henry Kurnia; Kahar, Neysa Marcella; Stephanie, Stephanie; Sukarti, Emi
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 7 No. 1 (2020): Februari
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v7i1.2389

Jamu/Herbal Medicine that contain chemical compounds/Pharmaceutical compounds has been banned by The National Agency of Drug and Food Control in Indonesia, but in December 2017, BPOM released a public warning about aphrodisiac herbal medicine that contain chemical compound like sildenafil citrate and phenylbutazone in the market. The aim of this study was to find out the valid method for identification of sildenafil citrate and phenylbutazone in aphrodisiac herbal medicine. In this study, the developed method was applied to 22 brands of aphrodisiac herbal medicine sample. Sample was extracted with methanol, then 5 µl of sample spotted on silica gel plate 60 F 254 and eluated by chloroform : methanol : amonia (70 : 3 : 1.5, v/v/v). The spots were analyzed by TLC scanner at λ 286 nm. Sildenafil citrate was appeared at Rf 0.76; tadalafil at 0.6 and phenylbutazone at Rf 0.12 with resolution (Rs) value of sildenafil and tadalafil was 1.1 meanwhile tadalafil and phenylbutazone was 5.4. Limit of detection of sildenafil citrate was 8.552 µg/ml (2.053 mg/600 mg capsul), tadalafil was 1.629 µg/ml (0.391 mg/600 mg capsul), and phenylbutazone was 7.877 µg/ml (1.890 mg/600 mg capsul). This study found 14 brands that gave a positive result containing sildenafil citrate and no sample contain tadalafil and phenylbutazone.

Standarisasi Simplisia Daun Kenikir (Cosmos caudatus Kunth) Dari Tiga Daerah Berbeda Jannah, Miftahul; Wijaya, Sumi; Setiawan, Henry Kurnia
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 8 No. 1 (2021): Februari
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v8i1.3087

Kenikir dipercaya sebagai obat penurun suhu tubuh, memperbaiki sirkulasi darah, mengobati diabetes, sebagai anti-aging dan menjaga kekuatan tulang. Saat ini telah banyak produk serbuk daun kenikir dalam bentuk kapsul di pasaran, tetapi belum ada data standarisasi dari simplisia daun kenikir, sehingga penelitian ini dilakukan bertujuan untuk mendapatkan profil standarisasi spesifik dan non-spesifik dari simplisia daun kenikir. Parameter spesifik terdiri dari identitas simplisia, organoleptis, kadar sari laut air, kadar sari larut etanol, penetapan profil kromatografi dengan menggunakan KLT, penetapan profil spektrum dengan menggunakan spektrofotometer infrared dan UV-Vis serta penetapan kadar senyawa metabolit sekunder. Parameter non spesifik meliputi susut pengeringan, kadar abu total, kadar abu tak larut asam, kadar abu larut air, pH, dan persen bahan asing. Data yang diperoleh merupakan data deskriptif yang diperoleh dari 3 lokasi berbeda. Hasil pengamatan mikroskop daun segar kenikir menunjukkan tipe berkas pembuluhnya adalah kolateral terbuka, jaringan bunga karang atau palisade, dengan tipe stomata anomositik, rambut penutup non-glandullar, kristal Ca-oksalat bentuk pisma dan tipe daun dorsiventral. Hasil kadar sari larut etanol > 23,3%, kadar sari larut air > 13,15%. Hasil skrining fitokimia menunjukkan adanya senyawa flavonoid, saponin, polifenol, steroid dan triterpenoid. Hasil pengamatan profil kromatogram secara KLT dengan fase diam silika gel F254 dan fase gerak yang terpilih adalah n-heksan : etil asetat (7:3). Hasil penetapan kadar flavonoid total > 1,30%, fenol > 2,18%. Nilai susut pengeringan ≤13,5%, sedangkan hasil kadar abu total ≤6,0% dengan kadar abu larut air ≤5,0% dan kadar abu tak larut asam ≤2,0%, pH simpisia untuk pelarut air yaitu 6- 7 dan pada pelarut etanol 4 – 6.

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