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All Journal JURNAL PETERNAKAN JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Triton Wahana Peternakan Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Jurnal Embrio
Mangku Mundana
Fakultas Peternakan, Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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The Effect of GnRH on Reproductive Performance and Progesterone Hormone Levels in Buffalo in West Sumatera Tinda Afriani; Ferdinal Rahim; Mangku Mundana; Arif Rahmat; Jaswandi; Anna Farhana
JURNAL TRITON Vol 11 No 2 (2020): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v11i2.110

Abstract

The administration of hormone GnRH will affect the reproductive organs and can improve the reproductive performance of buffalo. The decrease of the buffalo population is caused by various factors including genetic and environmental factors. The development of buffaloes is relatively slow because the reproduction rate of buffaloes is lower than that of cattle, besides that it is also difficult to detect estrus, the pregnancy period is relatively long and longer calving rate. This study aims to determine the best dose of GnRH on the swamp buffalo producing of dadih in Indonesia. The materials used were 16 swamp buffalo. A completely randomized design was used in this study. Buffalo is synchronized with the injection of 5 ml of GnRH hormone n on the first and 11th day, on the 10th to 12th day all donor buffaloes are injected with GnRH. Four different doses of GnRH (200, 225, 250 and 275 ml/buffalo) and each treatment was replicated five times. The speed of estrus and estrus length were the measured variables. The results showed that the doses of GnRH significantly (P<0.05) increase of estrus speed and estrus length. Progesterone levels have increased significantly starting from before treatment until after treatment with GnRH. It is concluded that the best GnRH doses for estrus synchronization in the swamp buffalo producing of dadih was 2. 25 ml/buffalo with estrus speed 18.2 h and estrus length 18 h.
GAMBARAN DAN STRUKTUR POPULASI TERNAK KERBAU PADA PETERNAKAN RAKYAT DI SIJUNJUNG, SUMATERA BARAT ferry lismanto; Mangku Mundana; Fauzani Hurriya Revar
Jurnal Embrio Vol 12 No 2 (2020): Jurnal Embrio
Publisher : Fakultas Pertanian Universitas Tamansiswa Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.307 KB) | DOI: 10.31317/embrio.v12i2.616

Abstract

Tujuan penelitian ini adalah untuk mengetahui gambaran dan struktur populasi ternak kerbau di daerah Sijunjung, Sumatera Barat. Penelitian ini dilaksanakan Kecamatan Sijunjung Kabupaten Sijunjung.Metode yang digunakan pada penelitian ini adalah metode survei. Sedangkan teknik pengampilan sampel adalah simple random sampling. Selanjutnya teknik penetapan sampel menggunakan snowball sampling. Responden yang digunakan pada penelitian ini sebanyak 145 responden. Variabel yang diamati adalah karakteristik peternak, gambaran dan struktur populasi kerbau. Data dianalisis secara deskriptif.Perolehan hasil penelitian yaitu jumlah kerbau jantan pada umur 0-1 tahun ;>1-2,5 tahun; jantan dewasa ≥ 3,5 tahun yakni sebesar 73 ekor (6,2%); 123 ekor (10,4%) dan 116 ekor (9,9%). Namun jumlah kerbau betina berumur 0-1 tahun; >1-2,5 tahun; betina dewasa ≥ 3,5 tahun yakni sebesar 73 ekor (6,2%); 2015 ekor (17,5%) dan 585 ekor (49,7%). Sedangkan imbangan/rasio jantan dan betina sebesar 1:6. Sedangkan tingkat iput kerbau sebesar 179 ekor(15,1%), sedangkan output kerbau sebesar 166 wkor (14,1%). Dapat disimpulkan dari penelitian ini bahwa populasi kerbau terbanyak di daerah Sijunjung adalah kerbau betina sebanyak 73,4% dengan angka kelahiran sebesar 14,6% dan namun tingkat kematian lebih tinggi sebesar 15,1% yang terdapat pada gudel. Tingginya tingkat kematian gudel dibandingkan kelahiran menyebabkan rendahnya perkembangan populasi kerbau di daerah Sijunjung.
Identifikasi Keragaman Gen FSH Bagian Ekson 2 Menggunakan Enzim Restriksi TasI pada Sapi Pesisir Tinda Afriani; Endang Purwati; James Hellyward; Jaswandi Jaswandi; Yurnalis Yurnalis; Mangku Mundana; Anna Farhana; Adisti Rastosari
Jurnal Peternakan Vol 19, No 2 (2022): September 2022
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v19i2.17228

Abstract

ABSTRAK. Studi ini bertujuan untuk mengidentifikasi keragaman gen FSH bagian ekson 2 menggunakan enzim restriksi TasI pada sapi Pesisir. Sampel darah yang digunakan yaitu 78 sampel darah dengan dua macam yaitu 13 jantan serta 65 betina yang berumur 1-5 tahun dari sapi pesisir yang didapat dari BPTUHPT Padang Mengatas. Protocol genomic DNA Purification system Kit dari Promega digunakan untuk mengisolasi DNA sampel darah. Amplifikasi gen FSH dilakukan dengan menggunakan teknik PCR atau Polymerase Chain Reaction. Enzim TasI digunakan untuk meretriksi produk amplifikasi. Hasil penelitian gen FSH bagian exon 2 pada sapi pesisir yaitu diperoleh terdapat dua macam genotip. Dua macam genotip tersebut yaitu heterozigot (+/-) serta homozigot tidak terpotong (-/-), untuk jantan berturut turut yaitu 0,38 dan 0,62 beserta frekuensi alel (+) dan alel (-) yaitu 0,19 dan 0,81, sedangkan untuk betina yaitu berturut-turut 0,43 dan 0,57 beserta frekuensi alel (+) dan alel (-) yaitu 0,21 dan 0,79. Sebaran genotip gen FSH|TasI exon 2 pada populasi Sapi Pesisir sifatnya polimorfik (beragam) dengan frekuensi alel yaitu kurang dari 0,99.Identification of Exon 2 FSH Gene Diversity Using TasI Restriction Enzymes in Coastal CattleABSTRACT. This study aims to identify the diversity of the exon 2 FSH gene using the restriction enzyme TasI in Coastal cattle. The blood samples used were 78 blood samples of two types, namely 13 males and 65 females aged 1-5 years, from coastal cattle obtained from BPTUHPT Padang Atas. The protocol genomic DNA Purification System Kit from Promega was used to isolate DNA from blood samples. The amplification of the FSH gene was carried out using the PCR or Polymerase Chain Reaction technique. The TasI enzyme was used to restrict the amplification product. The study results on the exon two portion of the FSH gene in coastal cattle showed two types of genotypes. The two types of genotypes are heterozygous (+/-) and untruncated homozygous (-/-); for males, 0.38 and 0.62, respectively, and the frequencies of allele (+) and allele (-) are 0.19 and 0. .81, while for females, it was 0.43 and 0.57, respectively, and the frequencies (+) and allele (-) were 0.21 and 0.79. The FSH|TasI exon two gene genotype distribution in the Coastal Cattle population is polymorphic (various) with an allele frequency of less than 0.99.Keywords: FSH Gene, Pesisir Cattle, TasI Enzyme
Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle Tinda Afriani; Endang Purwati; James Hellyward; Jaswandi Jaswandi; Mangku Mundana; Anna Farhana; Adisti Rastosari
JURNAL ILMIAH PETERNAKAN TERPADU Vol 10, No 3 (2022)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v10i3.p264-276

Abstract

The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.
KERAGAMAN GEN FOLLICLE STIMULATING HORMONE (FSH|TasI) EXON 2 PADA SAPI PESISIR: Diversity of the Follicle Stimulating Hormone (FSH|TasI) Exon 2 Gene in Coastal Cattle Yurnalis Yurnalis; Mangku Mundana; Teguh Rafian
Wahana Peternakan Vol. 7 No. 3 (2023): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v7i3.1136

Abstract

Penelitian ini bertujuan untuk mengetahui keragaman Gen Follicle Stimulating Hormone (FSH|TasI) exon 2 pada sapi Pesisir. Penelitian ini dilakukan pada bulan 2 Juli sampai 21 September 2020 di Laboratorium Bioteknologi Ternak. Metode yang digunakan dalam penelitian ini adalah metode eksplorasi. Sampel yang digunakan dalam penelitian ini sebanyak 78 sampel darah Sapi Pesisir (13 jantan dan 65 betina) yang diambil di Balai Pembibitan Ternak Unggul Hijauan Pakan Ternak (BPTUHPT) Padang Mengatas, Kabupaten Lima Puluh Kota. Sampel darah diisolasi menggunakan protokol genomic DNA Purification Kit dari Promega. DNA hasil isolasi kemudian di amplifikasi menggunakan sepasang primer Forward: 5-’TCTCAGTTTTCTACAAGCCTT-3’ dan Reverse 5’-GGGAATCAATGAAGCCTGCC-3’ yang menghasilkan fragmen gen FSH ekson 2 sepanjang 271 bp. Produk amplifikasi direstriksi menggunakan enzim TasI yang mengenali situs pemotongan (AA↓TT). Hasil Penggenotipan gen FSH menggunakan enzim TasI pada sapi Pesisir ditemukan dua macam genotip yaitu 44 individu (8 jantan dan 36 betina) bergenotip homozigot tidak terpotong (-/-) dan 32 individu (5 jantan dan 27 betina) bergenotip heterezigot (+/-). Berdasarkan dari hasil analisis data, diperoleh dua macam genotip yaitu heterozigot (+/-) sebesar 0,38 dan homozigot tidak terpotong (-/-) sebesar 0,62 dengan frekuensi alel (+) sebesar 0,19 dan alel (-) sebesar 0,81 untuk sapi jantan dan heterozigot (+/-) sebesar 0,43 dan homozigot tidak terpotong (-/-) sebesar 0,57 dengan frekuensi alel (+) sebesar 0,21 dan alel (-) sebesar 0,79 untuk sapi betina. Dari hasil penelitian ini, dapat disimpulkan bahwa gen FSH pada populasi Sapi Pesisir yang diteliti bersifat polimorfik dan berada dalam keseimbangan Hardy-Weinberg. Kata kunci: Gen FSH, Sapi Pesisir, Enzim TasI
Optimasi Penggunaan Tanaman Indigofera sebagai Pakan Tambahan Ternak pada Kelompok Tani Jirek Nagari Koto Gadang Kabupaten Agam Tinda Afriani; Khasrad Khasrad; Reswati Reswati; Mangku Mundana; Mylaufa Asyraf
Warta Pengabdian Andalas Vol 31 No 3 (2024)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.31.3.628-633.2024

Abstract

Indigofera plants have significant potential to enhance livestock productivity and agricultural sustainability. Community services on using Indigofera plants as supplementary livestock feed have been conducted in the 50 Kota District. This activity aims to increase farmers' knowledge about the benefits of Indigofera plants and their cultivation techniques. The method used involves forming a service team and meeting with farmer groups. The program included information on the benefits of Indigofera plants, cultivation techniques, maintenance, and harvesting. Practical field training was also provided to farmers. The program resulted in a positive response from farmers, who actively participate in the education activities. They understood the potential of Indigofera plants in improving livestock productivity. These plants are nutrient-rich and can be used as a high-quality feed source. They are using Indigofera as supplementary livestock feed, which has a high potential to support healthy and efficient livestock production. This activity is a crucial step in promoting sustainable agriculture and livestock farming.
Co-Authors Adisti Rastosari Anna Farhana Anna Farhana Arif Rahmat Endang Purwati RN Fauzani Hurriya Revar Ferdinal Rahim Ferry Lismanto Syaiful James Hellyward Jaswandi . Khasrad . Mylaufa Asyraf Reswati, Reswati Teguh Rafian Tinda Afriani Yurnalis Yurnalis