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All Journal BIOMA : Jurnal Biologi Makassar Jurnal Ilmiah Kesehatan Rustida Jurnal Penelitian Farmasi dan Herbal Journal SAGO Gizi dan Kesehatan Insight Power (Kekuatan Wawasan) - Jurnal Pengabdian Kepada Masyarakat Digital (JUPED) Jurnal Pengmas Kestra (JPK)
SAADAH SIREGAR
INSTITUT KESEHATAN MEDISTRA LUBUK PAKAM
Agriculture, Biological Sciences & Forestry Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Other

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ANTIBAKTERI SENYAWA FENOLIK DARI DAUN SAPUTANGAN (Maniltoa Grandiflora (A. Gray) Scheff) JHON PATAR SINURAT; SAADAH SIREGAR
Jurnal Penelitian Farmasi & Herbal Vol 1 No 2 (2019): Jurnal Penelitian Farmasi & Herbal
Publisher : Fakultas Farmasi Institut Kesehatan DELI HUSADA Deli Tua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36656/jpfh.v1i2.64

Abstract

The Phytochemical screening test to identify secondary metabolites of Saputangan leaves extract (Maniltoa grandiflora (A.Gray) Scheff) showed positive results on phenolic and terpenoid compounds. The reagent used for phenolic screening was 5% FeCl3 and reagent for screening terpenoid was 1% CeSO4 in 10% H2SO4. Antibacterial test was carried out by agar diffusion method on extracts of DMSO Saputangan leaves. Phenolic compound obtained from extract of Saputangan leaves were 36.96 gram where the result was obtained after maceration, partitioning and evaporation processes. Antibacterial of phenolic compound was observed based on inhibitory zone diameters of phenolic compounds formed using paper discs with a diameter of 6 mm. The diameters of the inhibition zone against Escherichia coli bacteria are 8.1; 8.5 and 9.7 mm larger than the diameter of the inhibition zone in Staphylococcus aureus bacteria at 7.3; 7.8 and 8.5 mm. Then it can be stated that DMSO extracts of phenolic compound with concentrations (0.25; 0.50 and 1.00 mg / ml) have antibacterial strength at the medium level where the average inhibition zone of S. aureus bacteria is 7.87 mm and the average inhibition zone in E. coli bacteria is 8.76 mm.
Sosialisasi Efektifitas Penggunaan Pewarna Alternatif Preparat Permanen Telur Nematoda Kolon Menggunakan Pewarna Rhodamin B Saadah Siregar; Vincentia Ade Rizky; Asvia Rahayu; Visensius Krisdianilo
Jurnal Pengabdian Kepada Masyarakat Digital Vol 1, No 3: JUPED - Agustus 2022
Publisher : Insight Power (Kekuatan Wawasan)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.358 KB)

Abstract

Staining is one way to do research to identify eggworms. The dyeing technique explains the material and description of the  worm egg under the microscope and helps to distinguish it from the surrounding feces. A widely used method for staining  histological tissue with hematoxylin and eosin. A commonly used stain for the above colon nematode examination  is eosin stain. Rhodamine B dye is used instead of eosin dye to add a reference dye. The method used is experimental and uses eosin-controlled rhodamine B concentration ratios of 0.5%, 1%, 1.5%, and 2% to 3%. The  worm egg morphology is clearly visible from the outer layer to the inner layer, the  field of view of the rhodamine B dye is clearly visible, and the color contrast of the egg  is also clearly visible. From the research conducted, it can be concluded that Rhodamine B can be used as an alternative to the dye eosin. After the seminar, participants in the dedication activity realized that dye Rhodamine B  was used instead of dye eosin.Keywords: intestinal nematode egg, hematoxylin-eosin, rhodamine B
Perbedaan hasil pemeriksaan jumlah koloni bakteri pada daging ayam broiler dengan pemberian parutan serai (Cymbopogon citratus) setelah 24 jam Saadah Siregar; Vincentia Ade Rizky; Wenda Putri Natal Saragih
Jurnal SAGO Gizi dan Kesehatan Vol 4, No 1 (2022): Juli - Desember
Publisher : Politeknik Kesehatan Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v4i1.1073

Abstract

Background: Chicken meat is one of the most highly nutritious foodstuffs, as it contains nutrients that are indispensable to the human body, such as water, protein, fat, and other organic ingredients. However, the availability of these complete nutrients has caused the meat to become a medium for bacterial growth. Pathogenic bacteria and spoilage bacteria can cause health problems.Objectives: The study aims to determine the difference in examining the number of bacterial colonies in broiler chicken meat by giving lemongrass (Cymbopogon citratus) after 24 hours.Methods: Quantitative research using experimental design through laboratory testing, namely by determining whether or not there is a decrease in the number of germs in broiler chicken meat by giving grated lemongrass leaves after 24 hours. The sample is broiler chicken meat. The research was conducted in 2021. Laboratory testing was carried out using the pour plate method for two repetitions. Each plate of the dilution is calculated as the number of colonies by multiplying the dilution per 1 gram / 1 ml sample. Data were analyzed descriptively and bivariate using Independent T-test at 95% CI.Results: It was found that the growth of total germ numbers in chicken meat without incubation after 24 hours was treatment one was 39,96 x 106 CFU/gram, and treatment two was 39,87 x 106 CFU/gram, while chicken meat incubated for 24 hours was treatment one. The number of colonies in the first treatment was 40,39 x 106 CFU/gram, and 40,00 x 106 CFU/gram in treatment two. While chicken meat with lemongrass grater was incubated after 24 hours, there was a decrease in the number of colonies in the first treatment of 33,91 x 106 CFU/gram and the second treatment of 34,30 x 106 CFU/gram.Conclusion: There was a decrease in the number of germs in broiler chicken meat given grated lemongrass leaves after 24 hours of incubation.
Identifikasi Bakteri Coliform pada Susu Kedelai Menggunakan Metode MPN (Most Probable Number) Vincentia Ade Rizky; Saadah Siregar; Asvia Rahayu; Visensius Krisdianilo; Suci Mustika Mawardani; Dian Pratiwi
Jurnal Ilmiah Kesehatan Rustida Vol 10 No 1 (2023): Januari
Publisher : Akademi Kesehatan Rustida

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55500/jikr.v10i1.191

Abstract

Escherichia coli yaitu bakteri yang hidup diusus manusia, sebagai flora normal. Akan tetapi bakteri ini juga sering mengkontaminasi dan dapat menyebabkan penyakit pada manusia. Pengolahan pangan yang tidak hygienis dapat menyebabkan berkembangnya bakteri ini. Penelitian ini bertujuan untuk mengidentifikasi adanya bakteri Coliform pada susu kedelai yang disimpan disuhu kulkas dan suhu ruangan metode MPN (Most Probable Number). penelitian ini bersifat deskriptif analitik dengan populasi susu kedelai yang disimpan disuhu kulkas dan suhu ruangan pada penyimpanan ½, 1, 1 ½, dan 2 hari. Berdasarkan hasil pengujian dengan metode presumptive test, confirmative test dan complete test didapatkan hasil pada penyimpanan disuhu kulkas penyimpanan ½ hari dan 1 hari sampel 1,2,3,4,5 yaitu <3 CFU/mL, penyimpanan 1 ½ hari sampel 1, 2 dan 3 didapatkan 15 CFU/mL, sampel 4 dan 5 yaitu 11 CFU/mL. Penyimpanan 2 hari didapatkan 20 CFU/mL. Sedangkan pada penyimpanan disuhu ruangan ½ hari sebesar <3 CFU/mL, penyimpanan 1 hari pada sampel 1,2 dan 3 yaitu 11 CFU/mL, sampel 4 dan 5 yaitu 7 CFU/mL. Penyimpanan 1 ½ hari didapatkan sampel 1,2 dan 3 yaitu 21 CFU/mL, sampel 4 dan 5 yaitu 20 CFU/mL, penyimpanan 2 hari yaitu 28 CFU/mL. Pada penyimpanan ½ hari dan 1 hari pada penyimpanan disuhu kulkas serta penyimpanan pada suhu ruangan ½ hari dinyatakan tidak ada pengaruh dan layak dikonsumsi serta memenuhi syarat mutu, Sedangkan pada penyimpanan 1 ½ hari dan 2 hari pada suhu kulkas dan 1, 1 ½, dan 2 hari pada suhu ruangan terdapat pengaruh penyimpanan dan tidak memenuhi syarat mutu konsumsi.
SOSIALISASI AKTIVITAS DAYA HAMBAT EKSTRAK DAUN PIRDOT (SAURAIA VULCANI KORTH.) TERHADAP PERTUMBUHAN CANDIDA ALBICANS Romauli Anna teresia Marbun; Jhon Patar Sinurat; Saadah Siregar; Yanna Rotua Sihombing
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 2 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.125 KB) | DOI: 10.35451/jpk.v1i2.731

Abstract

Penyakit infeksi jamur menjadi perhatian khusus di berbagai negara dengan iklim tropis. Indonesia dengan iklim tropis disebabkan oleh udara yang lembab, dan sanitasi yang masih kurang. Indonesia memiliki lingkungan yang padat penduduk dan tingkat sosial ekonomi yang rendah. Jamur Candida albicans merupakan jamur penyebab kandidiasis dan penyebab sariawan, lesi pada kulit, vulvavaginistis, candida pada urin (kandiduria), dan gastrointestinal kandidiasis. Salah satu jenis tanaman yang berkhasiat sebagai obat yaitu pirdot (Sauraia vulcani Korth.) dan bagian yang biasanya digunakan adalah bagian daun. Penelitian sebelumnya ekstrak daun pirdot memiliki aktivitas menghambat pertumbuhan jamur Candida albicans. Konsentrasi daun pirdot 80% memiliki aktivitas daya hambat terhadap jamur yang paling efektif. Penelitian yang dilaksanakan tersebut menggunakan ketokonazol sebagai pembanding. Budidaya tumbuhan pirdot dapat dilakukan sendiri sehingga dapat digunakan untuk keperluan pribadi di tengah pandemic sekarang ini. Sasaran dari sosialisasi ini adalah mahasiswa Farmasi Institut Kesehatan MEDISTRA Lubuk Pakam. Hal ini menjadi penting untuk landasan penelitian selanjutnya untuk menghasilkan penelitian terkait produk antijamur ke depan
EFEKTIVITAS SEDIAAN GEL ANTISEPTIK TANGAN EKSTRAK DAUN SIRSAK Annona muricata Linn SEBAGAI ANTIBAKTERI TERHADAP Staphylococcus epidermidis VINCENTIA ADE RIZKY; SAADAH SIREGAR; VISENSIUS KRISDIANILO; SITI KHADIJAH
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Soursop leaf (Annona muricata Linn) has flavonoid, alkaloid, and saponin compounds that can be used as antibacterial. Based on the compounds contained in soursop leaves can be used as a hand antiseptic. Based on this study, an antiseptic gel formulation of soursop leaf (Annona muricata Linn) as antibacterial against Staphylococcus epidermidis bacteria with a carbomer 940 base was made. Antiseptic gel of soursop leaf extract was formulated with unequal extract concentrations, namely in the 1 3% formulation, the 2.6 % formulation. , formulation 3 9%, and formulation 4 12%. The antibacterial test used was the well diffusion method. The antiseptic gel was tested for its physical properties, namely organoleptic test, homogeneity test, dispersion test, and pH test. Antiseptic gel preparations that have antibacterial activity against Staphylococcus epidermidis bacteria are formulations 3 and 4 9% and 12% extracts which have a picture zone of 5 and 7 mm while the positive control has an inhibition zone of 12 mm. The results of the tests carried out on organoleptic tests, dispersion tests, pH tests, homogeneity tests were in accordance with the parameters of the gel preparation. From this study, it showed the ability of soursop leaves to inhibit the growth of Staphylococcus epidermidis bacteria. Soursop (Annona muricata Linn) leaf extract. Has antibacterial activity against Staphyloccus epidermidis bacteria with a concentration of 3% formulation 1, formulation 6% concentration 2, formulation concentration 9%, formulation concentration 12% 4. At a concentration of 3%, 6% has inhibitory power with a diameter of 3mm, and a concentration of 9% had an inhibitory power with an inhibitory diameter of 5mm, while the concentration of 12% had an inhibitory power with an inhibitory diameter of 7mm against the bacterium Staphyloccus epidermidis.
Co-Authors Asvia Rahayu Asvia Rahayu Dian Pratiwi Romaulianna Theresia Marbun Sinurat, Jhon Patar SITI KHADIJAH Suci Mustika Mawardani VINCENTIA ADE RIZKY Vincentia Ade Rizky Visensius Krisdianilo Visensius Krisdianilo Wenda Putri Natal Saragih Yanna Rotua Sihombing