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Amanah Amanah
Fakultas Kedokteran Universitas Swadaya Gunung Jati
Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology Nursing Public Health

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ISOLASI DAN IDENTIFIKASI MIKROFUNGI DERMATOFITA PADA PENDERITA TINEA PEDIS Amanah Amanah; Atik Sutisna; Ricardi Witjaksono Alibasjah
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 2, No 1 (2015): Tunas Medika Jurnal Kedokteran & Kesehatan
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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 Penelitian Isolasi dan Identifikasi Mikrofungi Dermatofita Pada Penderita Tinea Pedis telah dilakukan. Metode yang digunakan Metode Survei dan Deskriptif analisis. Pengambilan sampel uji dilakukan pada 3 pasien penderita Tinea Pedis dengan jenis klinis yang berbeda. Hasil dari  isolasi dan identifikasi pada pasien penderita Tinea corporis adalah mikrofungi dermatofita yang bersifat patogen  yaitu, Epidermophyton floccosum, Trichophyton mentagrophytes dan Trichophyton rubrum dan beberapa diduga mikrofungi yang bersifat  oportunis golongan kontaminan yaitu mikrofungi yang menempel pada inang seperti pada isolasi Aspergillus fumigates dan Aspergillus flavus.Kata Kunci: Isolasi dan Identifikasi, Dermatofita, Tinea PedisIsolation and Identification Research Mikrofungi dermatophytes in Patients with Tinea Pedis has been done . The method used survey method and descriptive analysis . Sampling was performed at 3 test Tinea Pedis patients with different clinical types . The results of the isolation and identification in patients with Tinea corporis is a dermatophyte mikrofungi that are pathogenic ie , Epidermophyton floccosum , Trichophyton mentagrophytes and Trichophyton rubrum and some opportunistic mikrofungi suspected contaminants are mikrofungi groups attached to the host as the isolation of Aspergillus flavus and Aspergillus fumigates . Key Words: Isolation and Identification, dermatophytes, Tinea Pedis
PENGARUH ANTIINFLAMASI EKSTRAK KULIT DURIAN (Durio zibethinus Murr) TERHADAP JUMLAH POLIMORFONUKLEAR NEUTROFIL PADA MENCIT YANG DIINDUKSI BAKTERI Eschericia coli Novianti Dwi Puspitasari; Rama Samara Brajawikalpa; Amanah Amanah
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 7, No 2 (2021): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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Perbandingan Efektivitas Minyak Atsiri Daun Sirih Hijau (Piper betle Linn) dengan Minyak Atsiri Rimpang Temulawak (Curcuma xanthorrhiza) terhadap Candida albicans secara In Vitro Amanah Amanah; Naufal Fadhil Mufid Lazuardi; Iwan Hermawan
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 4, No 2 (2018): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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ABSTRAK Candida albicans merupakan jamur penyebab keputihan dengan presentasi 75% wanita di seluruh dunia. Minyak atsiri daun sirih hijau mengandung karvakrol, eugenol dan kavikol, sedangkan minyak atsiri rimpang temulawak mengandung xanthorrhizol. Senyawa yang terdapat pada keduanya terbukti dapat menghambat pertumbuhan Candida albicans, membuat peneliti tertarik untuk meneliti masalah ini. Penelitian ini dilakukan untuk membandingkan efektivitas minyak atsiri daun sirih hijau dengan minyak atsiri rimpang temulawak terhadap Candida albicans secara in vitro. Dilakukan di Laboratorium Mikrobiologi FK UNSWAGATI, periode Januari-Februari 2018. Rancangan penelitian Postest Only Control Group Design. Pengambilan sampel dengan Purposive Sampling. Subyek penelitian minyak atsiri daun sirih hijau konsentrasi 50%, 25%, 12,5%, 6,25%, 3,125%, minyak atsiri rimpang temulawak konsentrasi 25%, 12,5%, 6,25%, 3,125%, 1,563%, Flukonzaol 25 µg (K+), dan DMSO 10% (K-). Data penelitian dianalisis secara statistik menggunakan uji non parametrik Kruskal Wallis dilanjutkan dengan uji Post-hoc Mann Whitney. Hasil rerata daya hambat minyak atsiri daun sirih hijau konsentrasi 50% (23,67 mm), 25% (19,67 mm), 12,5% (16,67 mm), 6,25% (15,33 mm), 3,125% (12,33 mm) dan minyak atsiri rimpang temulawak konsentrasi 25% (14,67 mm), 12,5% (11,67 mm), 6,25% (9,33 mm), 3,125% (6,33 mm), 1,563% (5,33 mm), K+ (13 mm), dan K- (11,33 mm). Hasil uji multifariat menunjukan nilai signifikansi p<0,05, terdapat perbedaan rerata antar dua kelompok perlakuan. Minyak atsiri daun sirih hijau terbukti lebih efektif dalam menghambat pertumbuhan jamur Candida albicans dibandingkan minyak atsiri rimpang temulawak. Kata kunci: Candida albicans, Minyak atsiri daun sirih hijau, Minyak atsiri rimpang temulawak
CALCULATING COLONY OF Candida spp. AMONG CHILDREN WITH INTELLECTUAL DISABILITY IN CIREBON Dhini Oktaviani; Amanah Amanah; Eni Suhaeni; Risnandya Primanagara
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Children and adults with Intellectual Disability and Autism Spectrum Disorders (ASD) have decreased intestinal permeability and enzyme activity, causing gastrointestinal (GI) problems that are more frequent and more severe than in children from the general population. The immune system which is part of the intestinal barrier in children with Intellectual Disability (ID) experiences various disorders and becomes more susceptible to fungal invasion, such as Candida spp. This present studyaims to calculate the colony of Candida spp. in the digestive tract of Children with Intellectual Disability (ID) in the city of Cirebon. Method: This is an observational study with cross-sectional design using purposive sampling. The study was conducted in November 2018-February 2019. The sample (ID) group consists of 31 individuals from Special Needs School and 60 individuals from National elementary school (non-ID) in the city of Cirebon. The inclusion criteria were children ages 6-12 years and the exclusion criteria were children under antifungal treatment and children under cytotoxic or immunosuppressive drugs. The fecalsamples from the ID group and non-ID were examined at the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati Cirebon, Indonesia using Digital Colony counter. Collected data were stored and analyzed using computer aided Statistical program. Mann-Whitney analysis of variance techniques were used to test the hypotheses. Results: The results showed that there are significant differences in the colonies of Candida spp. between the ID group and non-ID group. Median colony of Candida spp. in the ID group is 39.00 with mean colony of 126.90 while the median colony of non-ID group is 3.00 with mean colony of 40.93(p<0.001). Conclusion: There are significant differences between the Candida spp. colonies in the ID group with the non-ID. Keywords: Intellectual Disability (ID), Candida spp., Gastrointestinal
COMPARISON OF SOIL-TRANSMITTED HELMINTH EGG INFESTATION IN FECES OF INTELLECTUAL DISABILITY (ID) CHILDREN WITH NON-ID CHILDREN Mellyna Iriyanti Sujana; Amanah Amanah; Moch Yusuf Handoyo
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: The worm infestation is considered as one of the causes of cognitive function disruption. It also can aggravate the condition in children with intellectual disability (ID). This greatly affects health conditions, such as abdominal pain, bloating, diarrhoea, constipation and other digestive disorders. Indigestion may increase aggressive behaviour, mood change and malnutrition so that it leads to chronic malnutrition which is the cause of major morbidity and premature death in the ID population. This study aimed to compare soil-transmitted helminth egg infestation in stool samples between ID children and non-ID children. Methodology: This present study was a descriptive study with a cross sectional design. Stool samples was collected from 30 students from Special Need School and 60 students from Elementary School in the Cirebon City. The floating method was used to identify the egg of soil-transmitted helminth. The stool samples were examined in the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia Results: The laboratory test found positively Soil-transmitted Helminth eggs in 9 of 30 stool sample of ID students. Among them, it was found 5 stool samples with Ascaris lumbricoides, 2 stool samples of Trichuris trichiura, and 2 stool samples of Necator americanus. Regarding their levels, 4 students (44.4%) had mild intellectual disability and 5 students (55.6%) had moderate intellectual disability. However, there was not found any Soil-transmitted Helminth eggs in stool samples of non-ID children. Conclusion: Soil-transmitted helminth eggs was found only in stool samples of ID children. The hygiene and sanitation in the school should be guaranteed to prevent the transmission of Soil-transmitted Helminth. Key words: Soil-Transmitted Helminth, Intellectual Disability (ID), Feces
INHIBITORY TEST OF DAYAK ONION (ELEUTHERINE BULBOSA MILL.) ESSENTIAL OIL TO THE GROWTH OF MALASSEZIA FURFUR Syahidatun Hayati; Amanah Amanah; Rose Indriyati
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Certain fungi are normal flora on human mucosa and skin, and they require lipids for growth. One of them is Malassezia furfur, which can develop into an opportunistic infection and will take a long time to treat. The fungus may cause pityriasis versicolor, which cause scaly and discoloration of the skin. It is estimated that 40-50% from population in the tropical country is infected. Dayak onion (Eleutherine bulbosa Mill.) essential oil have the potential to treat fungal infections. This research aims to see the inhibitory potential of dayak onion essential oil (Eleutherine bulbosa Mill.) in inhibiting the growth of Malassezia furfur. Methods: This is an experimental study with a post-test control group design. The samples grouped into 7 groups. The first five treatment (P1 to P5) was given dayak onion (Eleutherine bulbosa Mill.) essential oil with a concentration of 3.125%,6.25%, 12.5%, 25%, and 50%, P6 (positive control) was given ketoconazole, and P7 (negative control) was given 10% DMSO. The inhibitory effect is measured from the diameter of the inhibitory zone, then analyzed using Kruskal-Wallis and Mann-Whitney tests. Result: The result showed that average diameter on 50% concentration is 9.25mm, 25% concentration is 7.5mm, 12.5% concentration have diameter of 4.5mm, 6.25% concentration have diameter of 3mm, 3.125% concentration with diameter of 1.75mm, positive control group with diameter of 20mm, and negative control group with diameter 0mm. Conclusions: Essential oil of dayak onion (Eleutherine bulbosa Mill.) shows inhibition of the growth of Malassezia furfur. The smallest inhibition zone diameter was 1.75 mm with concentration of 3.125%. The largest inhibition zone at a concentration of 50% with diameter of 9.25 mm. This finding showed potential effects towards antifungi treatment. Keywords: Eleutherine bulbosa Mill, Malassezia fufur, pityriasis versicolor.
COMPARISON OF EFFECTIVENESS BETWEEN CELERY JUICE (Apium graveolens L.) AND 2% MICONAZOLE TOWARDS THE GROWTH OF Malassezia furfur Ronaa Hammada; Witri Pratiwi; Shofa Nur Fauzah; Donny Nauphar; Amanah Amanah
Proceedings of the International Conference on Applied Science and Health No. 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Introduction: Pityriasis versicolor is caused by the fungi Malassezia furfur with a worldwide prevalence of 50%, including tropical countries, second only to dermatitis in Indonesia. Pityriasis versicolor is difficult to treat and requires long-term treatment. The disease has high recurrence risk and may cause drug resistance. 2% Miconazole is known to have long-term side effects; therefore, alternative treatment is needed. Several studies suggested that celery (Apium graveolens L.) contains active substances with anti-fungal properties. This paper aims to investigate the comparison of effectiveness between celery juice and 2% Miconazole towards the growth of Malassezia furfur. Methods: This is an in-vitro experimental study with post-test only control group design. The subjects were split into 5 groups which were given celery juice in 10% DMSO with the concentration of 10%, 20%, 30%, 40%, and 50%. A negative control group was given only 10% DMSO and the positive control group was given 2% Miconazole. The data were then analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The Kruskall-Wallis test showed all concentration of celery juice had antifungal effect with p=0.000 (p<0.05) and were effective in inhibiting the growth of Malassezia furfur. The Mann-Whitney test showed that the 50% celery concentration was as effective as 2% Miconazole in inhibiting the growth of Malassezia furfur (p=0.495). Conclusion: Celery juice (Apium graveolens L.) was effective in inhibiting the growth of Malassezia furfur with 50% concentration as the most effective concentration.
POTENTIAL ACCELERATING EFFECT OF Ageratum conyzoides L. LEAVES EXTRACT ON FIBROBLASTS DENSITY OF INCISION WOUND OF MALE WHITE MICE (Mus musculus) Mega Ayu Lestari; Ariestya Indah Permata Sari; Amanah Amanah
Proceedings of the International Conference on Applied Science and Health No. 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Wound treatment using traditional medicine has been known widely in various countries in the world. Ageratum conyzoidesL. is commonly known by the ancient people to treat wound due to its potential anti-inflammatory effect. This study aims determine the effect of Ageratum conyzoidesL. leaves extract on fibroblast density of incision wound of male white mice (Mus musculus). Methods: This post-test only control group design experimental study used 35 male white mice which were randomly divided into five groups, i.e. negative control group K(-), positive control group K(+) (10% povidone iodine), and treatment group P1, P2, and P3 that were each given billy-goat weed leaf with increasing dose (15%, 30%, and 45% respectively). On each day, the length of the incision was measured by a ruler. After 7 days, the mice were terminated to obtain wound tissue which were used to prepare H&E stained histopathological sections to observe fibroblast density. Non-parametric analyses using Kruskall-Wallis and Mann Whitney test were used to compare the wound length and fibroblasts density. Results: Lengths of incision wound between all pairs of groups at the 7th day are significantly different (p< 0.05) with group P3 showed the shortest one. Significant differences were also observed in fibroblasts density between group K(-) and K(+), K(-) and P1, K(-) and P2, K(-) and P3, K(+) and P3, P1 and P3, P2 and P3 (p< 0.05) with group P3 showed the highest density among all groups. Conclusions: Ageratum conyzoidesL. leaves extract 45% has more potential effect than povidone iodine 10% in accelerating healing process by enhancing fibroblasts density.
INHIBITORY EFFECT OF Sansevieria trifasciata L ON AERIAL PATHOGENIC MICROFUNGI IN TUTORIAL ROOMS Sharah Julia Andayani; Amanah Amanah; Tissa Octavira Permatasari
Proceedings of the International Conference on Applied Science and Health No. 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Air pollutants in a room can be caused by several things, such as microorganisms in the form of fungi. Fungi that dispersed in air with concentration>700 CFU/m3 can be categorized as air pollution which could lead to many symptoms of various human diseases An effort that may improve indoor air pollution is using anti pollutant plant such as Sansevieria trifasciata L. This study aims to determine the effectiveness of Sansevieria trifasciata L on the concentration of aerial pathogenic microfungi in the tutorial room in Faculty of Medicine Swadaya Gunung Jati University and identify the aerial pathogenic microfungi species in the tutorial room. Methods: This study was a quasi experimental research with pre and post-test group design. Eight tutorial rooms with 4 repetitions were tested for species microfungi growth using Saburoud Dextrose Agar (SDA) media in 32 petri discs. After 7 days of incubation, microfungi were identified and the colony form unit (CFU) number was counted. The data was analyzed using paired T test. Results: Ten aerial pathogenic microfungi growth were significantly (p = 0.000) inhibited by Sansevieria trifasciata L demonstrated by CFU number reduction from 54.18 – 204.94 CFU/m3 to 16.48 – 44.75 CFU/m3. Conclusions: Sansevieria trifasciata L effectively inhibited aerial pathogenic microfungi growth in tutorial rooms.
Co-Authors Ariestya Indah Permata Sari Atik Sutisna Dhini Oktaviani Donny Nauphar Eni Suhaeni Iwan Hermawan Mega Ayu Lestari Mellyna Iriyanti Sujana Moch Yusuf Handoyo Naufal Fadhil Mufid Lazuardi Novianti Dwi Puspitasari Rama Samara Brajawikalpa Ricardi Witjaksono Alibasjah Risnandya Primanagara Ronaa Hammada Rose Indriyati Sharah Julia Andayani Shofa Nur Fauzah Syahidatun Hayati Tissa Octavira Permatasari Witri Pratiwi