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All Journal Global Health Management Journal PREPOTIF : Jurnal Kesehatan Masyarakat Tunas Medika Jurnal Kedokteran & Kesehatan JPM: JURNAL PENGABDIAN MASYARAKAT
Rama Samara Brajawikalpa
Fakultas Kedokteran Universitas Swadaya Gunung Jati
Aerospace Engineering Humanities Astronomy Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Other

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PERBANDINGAN PENURUNAN KADAR ASAM URAT PADA PEMBERIAN EKSTRAK DAUN TEMPUYUNG (Sonchus arvensis ) DAN EKSTRAK DAUN SUKUN (Artocarpus altilis) PADA TIKUS WISTAR HIPERURISEMIA Waly, Tuswandi Ahmad; Nurbaiti, Nurbaiti; Brajawikalpa, Rama Samara
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 7, No 2 (2021): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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UJI AKTIVITAS PEMBERIAN EKSTRAK DAUN SUKUN (Artocarpus altilis (Park) Fosberg.) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS PUTIH JANTAN YANG DIBUAT HIPERURISEMIA Nia Hernanti; Ricardi Wicaksono Alibasjah; Rama Samara Brajawikalpa
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 5, No 1 (2019): Tunas Medika Jurnal Kedokteran & Kesehatan
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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ABSTRAKLatar Belakang : Hiperurisemia atau asam urat salah satu penyakit tidak menular dengan angka kejadian 30% di dunia. Di Indonesia hingga tahun 2013 mencapai 25,8%. Seiring perkembangan zaman terdapat 2 jenis terapi untuk asam urat. Yang pertama terapi modern menggunakan obat dan yang kedua dengan pengobatan herbal menggunakan ekstrak daun sukun.Tujuan : Mengetahui aktivitas pemberian ekstrak daun sukun (Artocarpus altilis (Park) Fosberg.) terhadap kadar asam urat pada tikus putih jantan yang dibuat hiperurisemia.Metode Penelitian : Penelitian ini merupakan penelitian experimental dengan menggunakan rancangan penelitian pre and post test with control group design. Sampel yang digunakan yaitu 36 ekor tikus putih jantan yang dibagi menjadi 6 kelompok dan setiap kelompok terdiri dari 6 ekor tikus putih jantan. Analisis yang digunakan adalah uji normalitas (Uji Shapiro Wilk) dilanjutkan dengan Uji T Berpasangan, Uji Anova dan Anova Lanjutan.Hasil : Data yang diperoleh dari hasil analisis dengan Anova menunjukan bahwa pemberian ekstrak daun sukun 0,125g, 0,25g, 0,375g dan 0,5g dapat menurunkan kadar asam urat pada tikus putih jantan (P<0,05). Dengan dosis 0,5g paling efektif menurunkan kadar asam urat dalam darah sebanyak -5,84.Simpulan : Ekstrak daun sukun dapat menurunkan kadar asam urat.Kata Kunci : Ekstrak daun sukun, Hiperurisemia, Asam UratABSTRACTBackground : Hyperuricemia or uric acid is one of the non-communicable diseases with a 30% incidence rate in the world. In Indonesia until 2013 reached 25.8%. Along with the development era there are 2 types of therapy for uric acid. The first modern therapy uses drugs and the second with herbal treatment using breadfruit leaf extract.Objective : To know the activity of giving breadfruit leaf extract (Artocarpus altilis (Park) Fosberg.) To uric acid level in male white rat made hyperurisemia.Research Method : This research is an experimental research using pre and post test design with control group design. The sample used was 36 male white rats divided into 6 groups and each group consisted of 6 male white rats. The analysis used is the normality test (Shapiro - Wilk Test) followed by Pair T test, Anova Test and Anova Continuation.Result : The data obtained from the analysis with Anova showed that giving 0,125g, 0,25g, 0,375g and 0,5g breadth extract can decrease uric acid level in male white rat (P <0,05). With a dose of 0.5g most effective lowering uric acid levels in the blood as much as -5.84.Conclusion : Breadfruit leaf extract can lower uric acid levels.Keywords : Breadfruit leaf extract, Hyperurisemia, Uric Acid
Uji Efektifitas Ekstrak Daun Tempuyung (Sonchus arvensis L.) untuk Menurunkan Kadar Asam Urat pada Tikus Putih Wistar Jantan yang Dibuat Hiperurisemia Rama Samara Brajawikalpa; Pahmi Budiman S. Basyir; Nuria Nirmala
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 4, No 2 (2018): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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ABSTRAKLatar Belakang: Kadar asam urat darah yang tinggi dapat menyebabkan gout. Pengobatan namun jika dikonsumsi terus menerus dapat mengakibatkan efek samping, oleh sebab itu diperlukan obat alternatif dengan efek samping minimal dan mudah didapat seperti tempuyung.Tujuan: Membuktikan dosis efektif ekstrak daun tempuyung terhadap penurunan kadar asam urat pada tikus putih jantan yang dibuat hiperurisemia.Metode: Penelitian eksperimental dengan pre-post test with control group design. Sampel menggunakan 36 ekor tikus putih jantan, terbagi menjadi 2 kelompok kontrol (K (-) diberi pakan standar + akuades dan K (+) diberi pakan tinggi purin + akuades) dan 4 kelompok perlakuan (pakan tinggi purin + ekstrak daun tempuyung dosis 0,14 mg/gBB, 0,21 mg/gBB, 0,28 mg/gBB, 0,35 mg/gBB).Hasil: Semua kelompok menunjukan rerata penurunan kadar asam urat yang signifikan (p<0,05). Dengan rerata penurunan kelompok P1 3,17 mg/dL, kelompok P2 4,326 mg/dL, kelompok P3 5,628 mg/dL, dan kelompok P4 6,271 mg/dL.Kesimpulan: Ekstrak daun tempuyung dosis 0,35 mg/gBB paling efektif dalam menurunkan kadar asam urat darah.Kata Kunci : Asam Urat, Ekstrak Daun Tempuyung, Hiperurisemia. ABSTRACTBackground: High level of blood uric acid can cause a gout. Allopurinol is the best choice of drug for decreasing uric acid level in human body but if consume continuously may lead to side effects, therefore an alternative medicine which easily obtained and has minimum side effect is necessary, such as the tempuyung leaves.Aims: Prove the effective dozen of tempuyung leaf extract for reduction of uric acid levels in white male rats made hyperuricemia.Method: Experimental research with Pre-post test with control group design. The samples were 36 male white rats. Rats was divided into 2 control groups ( K(-) are given standar feed+ aquades and K(+) are given high purine feed + aquadest ) and 4 treatment groups (high purine feed + extract tempuyung leaves dozen 0,14 mg/gBB, 0,21 mg/gBB, 0,28 mg/gBB, 0,35 mg/gBB).Result: All groups showed a significant average decrease in uric acid level (p<0,05) with an average declined in group P1 3,17 mg/dL, group P2 4,326 mg/dL, group P3 5,628 mg/dL, group P4 6,271 mg/dL.Conclusion: Tempuyung leaf extract with dozen 0,35 mg/gBB is more effective for reducing the uric acid level in blood.Keywords: Urid Acid, Tempuyung leaf extract, Hyperuricemia
Pengaruh Pemberian Ekstrak Etanol Bawang Putih terhadap Kadar Kolesterol Total, LDL Dan HDL pada Tikus Putih Hiperkolesterol Rama Samara Brajawikalpa; Mirella Gresyalli Kautama
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 3, No 3 (2016): Tunas Medika Jurnal Kedokteran & Kesehatan
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LATAR BELAKANG: Kolesterol merupakan salah satu jenis lemak yang diproduksi oleh hati dan sangat diperlukan oleh tubuh, tetapi jika terjadi peningkatan kolesterol serum di atas batas normal akan menyebabkan hiperkolesterol.Apabila terjadi dalam jangka panjang menyebabkan terbentuknya gumpalan lemak dalam pembuluh darah sehingga dapat berisiko atherosklerosis. Obat golongan statin merupakan obat pilihan utama dalam menurunkan profil lipid pada kasus hiperkolesterol akan tetapi jika dikonsumsi terus menerus secara tidak langsung dapat mengakibatkan efek samping sehingga diperlukan alternatif terapi yang mempunyai mekanisme kerja menurunkan profil lipid pada pasien hiperkolesterol.Tanaman bawang putih mengandung senyawa Allicin, niasindan vitamin c.TUJUAN: Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol bawang putih terhadap kadar kolesterol total, LDL, HDL pada galur wistar.METODE: Metode penelitian ini penelitian eksperimental dengan Pre and Post Test With Control Group Design menggunakan 40 ekor tikus galur wistar dibagi kedalam delapan kelompok. Kelompok kontrol normal, kelompok kontrol negatif, kelompok kontrol positif, kelompok perlakuan 1, 2, 3, 4 dan 5 diberikan dosis ekstrak bawang putih masing-masing 3,6mg, 7,2mg, 10,8mg, 14,4mg dan 18mg.HASIL: Setelah perlakuan terdapat pengaruh pemberian ekstrak etanol bawang putih terhadapkadarkolesterol total, HDL dan LDL dengan nilai p<0,05.KESIMPULAN: Ekstrak etanol bawang putih memiliki pengaruh terhadap kadar kolesterol total, LDL dan HDL. Dosis 3,6mg/200g BB dapat menurunkan kadar kolesterol total, LDL dan meningkatkan HDL. Semakin tinggi ekstrak etanol bawang putih semakin besar penurunan kadar kolesterol total, LDL dan peningkatan HDL.Kata kunci: BawangPutih, Kolesterol Total, HDL, LDL BACKGROUND: Cholesterol is a type of fat produced by the liver and is needed by the body , but if there was an increase in serum cholesterol above normal limits will lead to hypercholesterolemia . In the event in the long term lead to the formation of fat clots in the blood vessels so that it can be risk to atherosclerosis. Statins are the drug of choice in lowering lipid profile in case of hypercholesterolemia if taken continuously can indirectly cause side effects necessitating alternative therapies that have a mechanism of action of lowering the lipid profile in patients with hypercholesterolemia.Garlic plant compounds Allicin, niasinand vitamin c.OBJECTIVE: The research aimed to determine the effect of ethanol extract of garlic on total cholesterol levels, LDL, HDL, the effective dose of ethanol extract of garlic in white galurwistar rats.METHOD: The research methods is an experimental research with Pre and Post Test With Control Group Designusing 40 galurwistar ratsdivided into eight groups. Normal control group, negative control group, positive control group, the treatment group 1, 2, 3, 4 and 5 is given a dose of garlic extract each 3,6mg, 7,2mg, 10,8mg, 14,4mg and 18mg.RESULT: After treatment there is the effect of ethanol extract of garlic on total cholesterol, HDL and LDL with p < 0.05.CONCLUSION: The ethanol extract of garlic has effects on total cholesterol, LDL and HDL. Dosage 3,6mg / 200g BB can lower total cholesterol, LDL and increase HDL. The higher the ethanol extract of garlic greater the reduction in total cholesterol, LDL and increase HDL. Keywords: Garlic, Total Cholesterol, HDL, LDL 
PENGARUH ANTIINFLAMASI EKSTRAK KULIT DURIAN (Durio zibethinus Murr) TERHADAP JUMLAH POLIMORFONUKLEAR NEUTROFIL PADA MENCIT YANG DIINDUKSI BAKTERI Eschericia coli Novianti Dwi Puspitasari; Rama Samara Brajawikalpa; Amanah Amanah
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 7, No 2 (2021): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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Efek Pemberian Ekstrak Daun Tempuyung (Sonchus arvensis L.) Terhadap Penurunan Kadar Asam Urat Pada Tikus Wistar Putih (Rattus norvegicus) Jantan Hiperurisemia Cindyyani Eka Putri; Rama Samara Brajawikalpa; Ruri Eka Maryam Mulyaningsih
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 5, No 1 (2019): Tunas Medika Jurnal Kedokteran & Kesehatan
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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Latar Belakang: Hiperurisemia adalah keadaan terjadinya peningkatan kadar asam urat darah di atas normal dan termasuk kedalam salah satu penyakit tidak menular yang angka kejadiannya masih tinggi mencapai 20% di dunia. Daun tempuyung (Sonchus arvensis L.) mengandung flavonoid sebagai penurun kadar asam urat melalui penghambatan kerja enzim xantin oksidase. Tujuan: Penelitian ini ditujukan untuk mengetahui dosis efektif ekstrak daun tempuyung terhadap penurunan kadar asam urat pada tikus wistar hiperurisemia. Metode: Metode Penelitian ini adalah penelitian eksperimental dengan pre-post test with control group design. Sampel menggunakan 30 ekor tikus wistar secara random dikelompokkan kedalam 5 kelompok secara Simple Random sampling. 2 kelompok kontrol (K- diberi pakan standar + akuades dan K+ diberi pakan tinggi purin + akuades) dan 3 kelompok perlakuan (pakan tinggi purin + esktrak daun tempuyung dosis 70 mg/200gBB, 75 mg/200gBB, dan 80 mg/200gBB). Periksa kadar asam urat pada 30 ekor tikus wistar , darah tikus wistar didapatkan melalui sinus orbitalis yang sudah dibius terlebih dahulu dengan menggunakan eter. Dan periksa kadar asam urat diukur dengan metode TBHBA. Hasil: Perbedaan bermakna (p<0,05) kadar asam urat sebelum dan sesudah perlakuan hanya ditunjukkan oleh kelompok perlakuan. Dengan rerata penurunan, Kelompok P1 2.5334 mg/dL, Kelompok P2 5.3993 mg/dL Kelompok P3 5.8599 mg/dL. Simpulan: Ekstrak daun tempuyung dosis 80 mg/gBB paling efektif menurunkan kadar asam urat darah.Kata Kunci: Daun Tempuyung, Asam Urat, hiperurisemia, tikus wistar, xantin oksidaseABSTRACTIntroduction: Hyperuricemia is a case of the increase of uric acid above normal levels of blood and includes to the one of non-communicable diseases which the number of events is still high, reaching 20% in the world. Tempuyung leaf (Sonchus arvensis L.) contains of flavonoids which has a benefit for decrease uric acid through the inhibition of enzyme xanthine oxidase action. Aim: This study aimed to knows the effective dose of tempuyung leaf extract to decrease uric acid levels in wistar rats hyperuricemic. Methods: This research methodology is Experimental research with Pre-post test with control group design. The samples were 30 wistar rats. Rats was divided into 2 control groups (K- are given standar feed+ aquadet and K+ are given high purine feed + aquadest ) and 3 treatment groups (high purine feed + extract tempuyung leaves dose 70 mg/200gBW, 75 mg/200gBW, 80 mg/200gBW). Check uric acid levels in 30 wistar rats, the blood of wistar rats is obtained through the orbital sinus which has been anesthetized first using ether. And check the uric acid level is measured by the TBHBA method. Resulsts: Significant differences (p <0.05) uric acid levels before and after treatment were only indicated by the treatment group. With a mean reduction in P1 2.5334 mg/dL, P2 group 5.3993 mg/dL, P3 group 5.8599 mg/dL. Conclusions: Tempuyung leaves extract with dose 80 mg/200gBW is most effective for reducing the uric acid level in blood.Keywords: Tempuyung leaves, Uric Acid, Hyperuricemic, Wistar rats, Xanthine oxidase.
Uji Efektivitas Antijamur Minyak Atsiri Daun Cengkeh (Syzygium aromaticum L.) terhadap Pertumbuhan Malassezia furfur Rama Samara Brajawikalpa; Aditya Naufal Ramzy
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 4, No 1 (2018): Tunas Medika Jurnal Kedokteran & kesehatan
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ABSTRAK Latar belakang: Malassezia  furfur merupakan jamur yang sering menyebabkan panu (pitiriasis versikolor). Pitiriasis versikolor tersebar luas secara universal, terutama ditemukan di daerah tropis yang bersuhu hangat dan lembab. Di Indonesia, pitiriasis versikolor (panu) menempati posisi kedua dermatomikosis yang tersering setelah dermatofitosis. Dalam rangka menghindari resistensi dari penggunaan antijamur topikal sintetik maka diperlukan alternatif antijamur topical. Salah satu tanaman herbal adalah daun cengkeh, dimana minyak atsiri daun cengkeh (Syzygium aromaticum L.) diketahui memiliki aktivitas sebagai antijamur.Tujuan: Penelitian ini bertujuan untuk mengetahui efektivitas antijamur minyak atsiri daun cengkeh (Syzygium aromaticum L.) terhadap pertumbuhan Malassezia furfur.Metode: Penelitian experimental in vitro dengan rancangan penelitian post-test control group design. Konsentrasi yang digunakan yaitu 25%, 50%, dan 75% dengan pelarut DMSO 10%.Hasil: Hasil penelitian ini menunjukkan bahwa rerata zona hambat kelompok konsentrasi 25% sebesar 15,67 mm, konsentrasi 50% sebesar 19,33 mm, konsentrasi 75% sebesar 26,67 mm Hasil analisis menunjukkan terdapat perbedaan zona hambat antar kelompokKesimpulan: Minyak atsiri daun cengkeh (Syzygium aromaticum L.) konsentrasi 75% mempunyai efek antijamur paling besar dibandingkan dengan konsentrasi 50% dan 25% dengan nilai p<0,05 Kata kunci: Antijamur, Malassezia furfur, Minyak atsiri daun cengkeh, Syzygium aromaticum L. ABSTRACT Background: Malassezia furfur is a common fungi that caused Pityriasis versicolor. In Indonesia, Pityriasis versicolor is the second most common dermatomycosis after dermatophytosis. In order to avoid the resistance of using synthetic topical antifungal. A herbal topical antifungal is needed. One of the herb is clove leaf, which is known to  have antifungal activity. Aims: This study aimed to determine the antifungal effectivity of cloves leaf's (Syzygium aromaticum L.) essential oil on Malassezia furfur growth.Method: The study was an experimental laboratory with post-test control group design. The clove leaf's essential oils were diluted to a concentration of 25%, 50%, and 75% with 10% DMSO. Kruskall-Wallis test was used to analyze the data followed by Mann-Whitney to examine the differential result between the groups.Result: The results of this study showed the average inhibition  rates are 15,67 mm for 25% concentration, 19,33 mm for 50% concentration, 26,67 mm for 75% concentration and 22,33 mm Conclusions: The cloves leaf (Syzygium aromaticum L.) essential oils with a concentration of 75% has the highest antifungal effect compared to 50% and 25% concentration (P<0,05). Keywords: Antifungal, Clove leaf essential oil, Malassezia furfur, Syzygium aromaticum L.
The Effectiveness of Cassava Leaf (Manihot esculenta C.) Flavonoid Quercetin as Dengue Virus-1 Antivirus in Vitro Rayasari, Husnaya; Apriyanto, Dadan Ramadhan; Brajawikalpa, Rama Samara; Dewi, Beti Ernawati
GHMJ (Global Health Management Journal) Vol. 7 No. 3s (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-741086

Abstract

Background:  Dengue fever is a mosquito-borne disease that can cause severe symptoms and death, placing high burden on healthcare systems in tropical regions. Currently, there are no antiviral treatments. It has been proven that synthetic quercetin able to improve body performance and reduce the risk of infection as well as inhibit DENV replication. Cassava leaves (Manihot esculenta C.) contain anthocyanins (flavonoids) such as quercetin and it shows potential as antiviral agent as the synthetic as well.    Aims: To describe the levels of self-compassion and procrastination among medical students and to examine how these factors relate to academic performance. Methods: This experimental study used post-test only with control group design consisted of 13 treatment groups and 1 control group. Treatment group were divided into 6 different concentrations for inhibition test (50 µg/mL, 25 µg/mL, 12.5 µg/mL, 6.25 µg/mL, 3.125 µg/mL, 1.5 µg/mL) and 7 different concentrations  for cytotoxicity test (80 µg/mL, 40 µg/mL, 20 µg/mL, 10 µg/mL, 5 µg/mL, 2.5 µg/mL, 1.25 µg/mL). The control group was a negative control treated with 0.2% DMSO. Inhibition was assessed by Focus Forming Unit Assay and cytotoxic was assessed by number of Microtiter Tetrazolium Assay (MTT Assay). The statistical analysis used in this study were the Shapiro-Wilk test for normality test, Kruskal-Wallis test for hypothesis test, and post-hoc test to determine whether the groups studied in this research had significant differences from each other. Results: The CC50 and IC50 values of quercetin of cassava leaf (Manihot esculenta C.) were found to be 3.44 μg/mL and 0.25 μg/mL, respectively, with an SI value of 123. Based on these results, it can be concluded that the flavonoid quercetin of cassava leaf (Manihot esculenta C.) exhibits selective properties in inhibiting the replication of DENV-1. Statistical analysis showed non-normal distribution (P<0.05), hypothesis test was accepted (P<0.05), and no significant differences between concentrations in the post-hoc test. Conclusion: Quercetin of cassava leaf (Manihot esculenta C.) is effective as an antiviral agent against dengue serotype 1 strain New Guinea C in vitro. Received: 25 September 2024  |  Reviewed: 15 October 2024  |  Revised: 30 November 2024  |  Accepted: 15 December 2024.   Dimension Badges:
Phytochemical Screening and Potency of Mango Peel Extract (Mangifera indica L.) var. Gedong Gincu in Inhibiting the Growth of Staphylococcus aureus ‘Adawiyah, Robi’atul; Apriyanto, Dadan Ramadhan; Brajawikalpa, Rama Samara
GHMJ (Global Health Management Journal) Vol. 8 No. 1s (2025): Special Issues
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-81s1156

Abstract

Background: Staphylococcus aureus is a pathogen most of which develop into Methicillin-resistant Staphylococcus aureus. To prevent bacterial resistance, herbal medicine is needed. Mango plants have secondary metabolite compounds that can inhibit bacterial growth. Gedong gincu mango is a specific mango variety that grows widely in Cirebon district. There has been no research that knows the secondary metabolite content and its potential as an antibacterial, especially the peel part which only becomes waste. Aims: To find out the chemical compounds contained and determine the potential of mango peel extract (Mangifera indica L.) var. gedong gincu in inhibiting the growth of Staphylococcus aureus. Methods: This research is an experimental with a posttest only control group design. Phytochemical screening test employed a qualitative method. The extract was made using the maceration method with 70% ethanol solvent. Antibacterial testing with well diffusion method, and given four treatment concentrations (W/V), namely 25%, 50%, 75%, 100%. The measurement on the inhibitory zone after 24 hours at temperature of 370C. Results: Gedong gincu mango peel extract contains secondary metabolite compounds flavonoids, tannins, saponins, and steroids. The inhibitory activity of gedong gincu mango peel extract with a concentration of 25% gedong gincu mango peel extract has an average inhibition zone of 11,55 mm, 50% average inhibition zone 13,55 mm, 75% average inhibition zone 14,88 mm, and 100% average inhibition zone 16,22 mm in inhibiting the growth of Staphylococcus aureus p(<0.05). Conclusion: Mango peel extract var. gedong gincu with a concentration of 25% has the potential to inhibit the growth of Staphylococcus aureus bacteria.
Effectivity Test of n-Hexane, Ethyl Acetate, and Butanol Fractions of Mango Peel (Mangifera indica L.) Gedong Gincu Variety on the Growth of Escherichia coli Siskanti, Alvina; Brajawikalpa, Rama Samara; Apriyanto, Dadan Ramadhan
GHMJ (Global Health Management Journal) Vol. 8 No. 1s (2025): Special Issues
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-81s1186

Abstract

Background: Escherichia coli was the most common cause of diarrhea. Diarrhea reached a high prevalent in West Java. The cause of antibiotic resistance to Escherichia coli was noncompliance medication. Mango peel (Mangifera indica L.) of the Gedong Gincu variety had secondary metabolites compound which act as antibacterial that can be used as alternative medication. Aims: To analyze phytochemicals and determine effectiveness of n-hexane, ethyl acetate, and butanol fractions of mango peel Gedong Gincu variety in the growth of Escherichia coli. Methods: This study used laboratory true experimental research with post-test only control group design. This research consisted 14 groups, divided into 2 control groups, namely control (+) given ciprofloxacin and control (-) given 10% DMSO, and 12 treatment groups given n-hexane, ethyl acetate, and butanol fractions concentration of Gedong Gincu 6.25%v/v, 12.5% v/v, 25% v/v, and 50% v/v. The test used well diffusion method on Mueller-Hinton Agar media. Results: The secondary metabolites in the n-hexane fraction included alkaloids, steroids, and saponins, while ethyl acetate and butanol fractions contained flavonoids, phenols, alkaloids, triterpenoids, and tannins. The results of the oneway ANOVA test obtained p<0.001 which indicated n-hexane, ethyl acetate, and butanol fractions were significantly inhibit the growth of Escherichia coli with the greatest mean inhibitory zones of 6.86 mm, 12.08 mm, and 9.35 mm, respectively. Conclusion: The most effective fraction of mango peel (Mangifera indica L.) Gedong Gincu variety in inhibit the growth of Escherichia coli was ethyl acetate fraction at a 50% concentration (12.08 mm).
Co-Authors Aditya Naufal Ramzy Amanah Amanah Apriyanto, Dadan Ramadhan Beti Ernawati Dewi Cindyyani Eka Putri Emallia Fitriani Marfuati, Sri Mirella Gresyalli Kautama Nasution, Aulia Ananta Nia Hernanti Novianti Dwi Puspitasari Nurbaiti Nurbaiti Nuria Nirmala Pahmi Budiman S. Basyir Putra, Ferdian Adhy Pratama Rayasari, Husnaya Ricardi Wicaksono Alibasjah Ruri Eka Maryam Mulyaningsih Siskanti, Alvina Waly, Tuswandi Ahmad Weni, Mustika Wisandoko, Ignatius Hapsoro Yulianti, Selsa ‘Adawiyah, Robi’atul