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All Journal Indonesian Journal of Cancer Chemoprevention Jurnal Ilmiah Teknik Mesin JFIOnline
. Sugiyanto
Lampung University
Biochemistry, Genetics & Molecular Biology Health Professions Mechanical Engineering Medicine & Pharmacology

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Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer Nurulita, Nunuk Aries; Meiyanto, Edy; Sugiyanto, .
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Gynura  procumbens  is  widely  used  as  traditional  remedy  in  South-East  Asia.  Gynura procumbens exhibites anti inflammatory, antioxidant, and reduced blood pressure activity. The aim of this study was to determine chromatographic profile of ethyl acetate fraction of  Gynura procumbens (FEG) and to investigate its cytotoxic properties and selectivity to colon cancerand breast cancer cancer cells. The chromatographic profile of FEG was determined using HPTLC densitometric  and  HPLC.  MTT  (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium  bromide) assay was performed to determine the growth inhibitory effect of FEG on the growth of WiDr, MCF-7, and T47D cells. NIH3T3, a normal cells was used to determine the selectivity of FEG, which  contained  small  amount  of  quercetin  as  identified  from  chromatographic  profile  both HPTLC  and  HPLC.  FEG  inhibited  cell  growth  of  WiDr,  of  MCF-7  and  of  T47D  cells  in  time dependent manner. Quercetin affected cell growth inhibition approximately two fold higher at WiDr and MCF-7, whereas FEG had lower effect on T47D cell. Quercetin did not seem as the main  active  compound  of  FEG.  At  this  study,  FEG  caused  less  inhibition  on  the  growth  of NIH3T3 cells than that of on all cell lines. Selectivity index (SI) of FEG on WiDr, MCF-7 and T47D were 4.97, 2.77 and 7.79 respectively. According to the datas obtained, FEG possesses moderate to high cytotoxicity properties on WiDr, MCF-7 and T47D cells. FEG demonstrates selective  effect  against  cancer  cells  and  reveals  prospective  properties  as  cancer chemoprevention agent.Keywords: Gynura procumbens, colon cancer, breast cancer, cytotoxicity, selectivity
Validasi Metode Penetapan Kadar Lisinopril dalam Spiked Plasma Secara Ultra Performance Liquid Chromatography Melalui Derivatisasi dengan 1-Fluoro 2,4 Dinitrobenzen Sumiyani, Ririn; Martono, Sudibyo; Sugiyanto, .
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 1 (2016)
Publisher : Indonesian Research Gateway

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ABSTRACT: A rapid, accurate, and sensitive method for determining lisinopril in spiked plasma was developed by means using an Ultra Performance Liquid Chromatography (UPLC) with 1-fluoro 2,4 dinitrobenzen (FDNB) derivatization. Lisinopril was precolumn derivatized with FDNB at optimum condition, i.e. room temperature and borate buffer at pH 11, subsequently analyzed with UPLC. Isocratic condition of acetate buffer (0.01 M, pH 3.50) : acetonitrile : metanol = 70 : 10 : 20 (v/v/v) as mobile phase, 0.3 mL/min of flow rate at λ 296 nm were applied at Acquity BEH C18 column, resulting a linearity of lisinopril at range of concentration of 5,0-100 ng/mL (Y = 410,59x + 211,91, r = 0.93). The accuration of the established method was achieved by 88,59±6,01 to 101,70± 2,56% recovery, while the precision was shown with RSD value of 2,57- 8,16 %, limit of detection (LOD) instrument of 0,73 ng/mL and limit of quatification (LOQ) 2,44 ng/mL, dwith R2 = 0,9987 dan r = 0,9993. In addition, the resulted LOD and LOQ more or less similar with the published HPLC-MS-MS method (1.03-10.0 ng/mL). Hence, it could be concluded that the developed UPLC method can be used as an alternative method for determining lisinopril in plasma.Keywords: Lisinopril, FDNB, derivatization, UPLCABSTRAK: Penetapan kadar lisinopril dalam spiked plasma secara Ultra Performance Liquid Chromatography (UPLC) melalui derivatisasi dengan 1-fluoro 2,4 dinitrobenzen (FDNB) merupakan metode yang cepat, sensitif dan akurat. Derivatisasi precolumn lisinopril dan FDNB optimum pada suhu kamar, suasana dapar borat pH 11,0, dilanjutkan analisis secara UPLC isokratis menggunakan kolom Acquity BEH C dan fase gerak dapar asetat (0,01 M pH 3,50): asetonitril:metanol (70: 10: 20, v/v/v), laju alir 0,3 mL/menit pada λ 296 nm, menghasilkan linieritas kadar lisinopril dalam spiked plasma pada rentang 5,0 -100 ng/mL terhadap luas area lisinopril-DNB dengan persamaan Y = 410,59x + 211,91 dengan R2 = 0,9987 dan r = 0,9993 Akurasi metode ditunjukkan dengan nilai % rekoveri sebesar 88,59±6,01 smpai dengan 101,70± 2,56 %. Ketelitian ditunjukkan dengan nilai RSD 2,57- 8,16 %, sedangkan Batas Deteksi Instrumen = 0,73 ng/ mL dan Batas Kuantitasi = 2,44 ng/mL. Hasil Batas Deteksi penelitian ini relatif sama dengan Batas Deteksi penetapan kadar lisinopril secara HPLC-MS (1,03- 10,0 ng/mL). Dengan demikian dapat disimpulkan bahwa metode ini berpotensi dikembangkan sebagai metode alternatif pengganti HPLC-MS untuk penetapan lisinopril dalam plasma.Kata kunci: Lisinopril, FDNB, derivatisasi, UPLC
Validasi Metode Penetapan Kadar Lisinopril dalam Spiked Plasma Secara Ultra Performance Liquid Chromatography Melalui Derivatisasi dengan 1-Fluoro 2,4 Dinitrobenzen Sumiyani, Ririn; Martono, Sudibyo; Sugiyanto, .
Jurnal Farmasi Indonesia Vol 8, No 1 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.293 KB) | DOI: 10.35617/jfi.v8i1.417

Abstract

ABSTRACT: A rapid, accurate, and sensitive method for determining lisinopril in spiked plasma was developed by means using an Ultra Performance Liquid Chromatography (UPLC) with 1-fluoro 2,4 dinitrobenzen (FDNB) derivatization. Lisinopril was precolumn derivatized with FDNB at optimum condition, i.e. room temperature and borate buffer at pH 11, subsequently analyzed with UPLC. Isocratic condition of acetate buffer (0.01 M, pH 3.50) : acetonitrile : metanol = 70 : 10 : 20 (v/v/v) as mobile phase, 0.3 mL/min of flow rate at λ 296 nm were applied at Acquity BEH C18 column, resulting a linearity of lisinopril at range of concentration of 5,0-100 ng/mL (Y = 410,59x + 211,91, r = 0.93). The accuration of the established method was achieved by 88,59±6,01 to 101,70± 2,56% recovery, while the precision was shown with RSD value of 2,57- 8,16 %, limit of detection (LOD) instrument of 0,73 ng/mL and limit of quatification (LOQ) 2,44 ng/mL, dwith R2 = 0,9987 dan r = 0,9993. In addition, the resulted LOD and LOQ more or less similar with the published HPLC-MS-MS method (1.03-10.0 ng/mL). Hence, it could be concluded that the developed UPLC method can be used as an alternative method for determining lisinopril in plasma.Keywords: Lisinopril, FDNB, derivatization, UPLCABSTRAK: Penetapan kadar lisinopril dalam spiked plasma secara Ultra Performance Liquid Chromatography (UPLC) melalui derivatisasi dengan 1-fluoro 2,4 dinitrobenzen (FDNB) merupakan metode yang cepat, sensitif dan akurat. Derivatisasi precolumn lisinopril dan FDNB optimum pada suhu kamar, suasana dapar borat pH 11,0, dilanjutkan analisis secara UPLC isokratis menggunakan kolom Acquity BEH C dan fase gerak dapar asetat (0,01 M pH 3,50): asetonitril:metanol (70: 10: 20, v/v/v), laju alir 0,3 mL/menit pada λ 296 nm, menghasilkan linieritas kadar lisinopril dalam spiked plasma pada rentang 5,0 -100 ng/mL terhadap luas area lisinopril-DNB dengan persamaan Y = 410,59x + 211,91 dengan R2 = 0,9987 dan r = 0,9993 Akurasi metode ditunjukkan dengan nilai % rekoveri sebesar 88,59±6,01 smpai dengan 101,70± 2,56 %. Ketelitian ditunjukkan dengan nilai RSD 2,57- 8,16 %, sedangkan Batas Deteksi Instrumen = 0,73 ng/ mL dan Batas Kuantitasi = 2,44 ng/mL. Hasil Batas Deteksi penelitian ini relatif sama dengan Batas Deteksi penetapan kadar lisinopril secara HPLC-MS (1,03- 10,0 ng/mL). Dengan demikian dapat disimpulkan bahwa metode ini berpotensi dikembangkan sebagai metode alternatif pengganti HPLC-MS untuk penetapan lisinopril dalam plasma.Kata kunci: Lisinopril, FDNB, derivatisasi, UPLC
PERILAKU CREEP PADA KOMPOSIT POLYESTER YUKALAC 157 BQTN-EX DENGAN FILLER SERAT GELAS Marlin, Dodi; Sugiyanto, .; Zulhanif, .
Jurnal Ilmiah Teknik Mesin FEMA Vol. 1 No. 1 (2013)
Publisher : FT UNILA

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Abstract

Research aims to investigate the creep behavior of the composite which made from polyester resin with fiberglass filler. Composites are composed from fiber glass with pararel oriented, then the composition of the fiber volume to matrix are vary at 5%: 95%, 10%: 90%, and 15%: 85%.Composite then casted based on dimensions listed in ASTM D2990. Composites tested with the tensile test to determine the tensile strength of the composite. The test is continue with creep testing by providing creep load on the testing 60% of the tensile strength. In order to know the mechanism of failure in the plane of composite fracture, SEM is used to observing it. The test result showed that the composite with the volume composition of the fiber versus matrix : 15 %: 85%, has the longest time of all variations although just only 1000 seconds.The low creep time due to presence of voids in the composite matrix, fiber breaking, pull out, debonding at the fiber and also the influence of loading during creep testing are not uniform.With a maximum creep only 1000 seconds, the composite results of this study are not feasible to be applied in both the shipping industry andautomotive.Keywords: polymer composite, Polyester, Fiber glass, creep behaviour
PERILAKU CREEP PADA KOMPOSIT POLYESTER DENGAN SERAT KULIT BAMBU APUS (GIGANTOCHLOA APUS (J.A & J. H. SCHULTES) KURZ) Taufik, M.Ikhsan; Sugiyanto, .; Zulhanif, .
Jurnal Ilmiah Teknik Mesin FEMA Vol. 1 No. 1 (2013)
Publisher : FT UNILA

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Abstract

Bamboo is used as raw material wicker and crafts are emerging as a new solution for use as new materials that are more environmentally friendly and have better mechanical strength. Research by utilizing bamboo as reinforcement fibers and polyester resin matrix aims to determine the mechanical strength of the resulting composite merging the two. Bamboo fiber composites made with various volume fractions of 10%, 15% and 20% using polyester resin and catalyst MEKPO ratio 100:1. The composite is prepared by hand lay-up by using a glass as a mold. Bamboo fibers used as reinforcement composites, is expected to increase the tensile strength and creep strength of each variation. In addition to observing the microstructure using SEM (Scanning Electron Microscope) to determine the failure mechanisms that occur on the fracture surface.From this study the authors concluded that bamboo fiber composites have the potential for further development because the tensile test results are directly proportional to the addition of fiber to the highest tensile strength was 86.01 MPa at 20% volume fraction, whereas the average creep testing best time of 529.63 seconds on 10% volume fraction, the failure mechanism of the plates shown in SEM is a form of fiber breaking, pull out and de bonding.Keywords: Bamboo, composite, creep.
PEMBUATAN OTOMASI PENGATURAN KERETA API, PENGEREMAN, DAN PALANG PINTU PADA REL KERETA API MAINAN BERBASIS MIKROKONTROLER Santoso, Ari Beni; Martinus, .; Sugiyanto, .
Jurnal Ilmiah Teknik Mesin FEMA Vol. 1 No. 1 (2013)
Publisher : FT UNILA

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This research is intended to study experimentally the relationship between automation systemsfor the transportation system, while the emphasis is on the point of train control system on the rails.The design of manufacturing automation systems in this research using a microcontroller PUATTiny 2313-20 for setting the working mechanism of the dynamo on a train and ArduinoDuemilanove to open or close the railway doorstop. The data obtained from this research arespeed, braking system that is safe, and the optocoupler output. Safe braking distances used inresearch was 10 cm. Keywords : Automation Systems, Microcontroller, Optocoupler, Braking Systems.
Pembuatan Sistem Otomasi Dispenser Menggunakan Mikrokontroler Arduino Mega 2560 Oktariawan, Imran; Martinus, .; Sugiyanto, .
Jurnal Ilmiah Teknik Mesin FEMA Vol. 1 No. 2 (2013)
Publisher : FT UNILA

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Abstract

Developments in science and technology in this era is an important factor and can not be separated in an attempt to improve the welfare of the community. This is proved by its increasement of peoples demand for tools that can work automatically, efficiently and saving energy. One of automation technologies that can be applied in home appliances is a dispenser that uses automation.Dispenser which used in this study using a microcontroller Ardunio Mega 2560, because the microcontroller can reduce the complexity of electronic circuits and instrumentation. The microcontroller pin is used as an input and 10 PIN 2 PIN as output. Meanwhile, in the manufacturing process of hardware includes five series are: power supply circuit, sensor circuit height glass, circuit microcontroller arduino mega 2560, Soil moisture sensor connection, relay driver circuit. While data that obtained are the water level, the presence sensor cups, and level glass.Automation system Arduino Mega 2560 microcontroller running well as the dispenser system mechanism. Resulting in a dispenser that can provide comfort for people, particularly in meeting the needs of drinking.Keywords : microcontrollers, Arduino Mega, Soil moisture sensor, Driver delay
ANALISA PERILAKU MEKANIK KOMPOSITSERAT KAPUK RANDU MENGGUNAKANMATRIK POLYESTER Sarifudin, Syamsul Arif; Tarkono, .; Sugiyanto, .
Jurnal Ilmiah Teknik Mesin FEMA Vol. 1 No. 2 (2013)
Publisher : FT UNILA

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The background research is the development of science and technology in the field of materials engineering and the demands off indinga new breakthrough in the provision of high quality materials and environmentally friendly. Composite materials of natural fibers that are more light weight, malleable, corrosion resistance, low price andeasy to obtain. This study uses materials such aspolyesterresin, and a catalyst Randu Kapok fiber. Randomly arranged fibers incomposites with various volume fractions of 15%, 25%, 35%. Making way press mold, bending tests were conducted with a reference standard ASTM D790-02, tensile testing standard ASTM D-638. Testing through the process of tensile and bending tests to determine the mechanical properties of the composite. Greatest tension obtained in the composite with 35% volume fractionis equal to 3,8046 MPa while the largest bending strength of the composite obtained from volume fraction 15% at 84MPa. Void formed causing the load can be held by thematrixis reduced due tolack of homogenan specimens.Keywords: Composite, Kapok Fiber, Polyester, Mechanic strength, Tensile Test and dan BendingTest.
STUDI SIFAT MEKANIK KOMPOSIT SERAT SANSEVIERIA CYLINDRICA DENGAN VARIASI FRAKSI VOLUME BERMATRIK POLYESTER Fajri, Rahmat Iskandar; Tarkono, .; Sugiyanto, .
Jurnal Ilmiah Teknik Mesin FEMA Vol. 1 No. 2 (2013)
Publisher : FT UNILA

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This study aims to create and analyze the tensile strength of the material matrix composite reinforced polyester resin natural fiber Sansevieria cylindrica leaves that will eventually be in seek of new composite materials which might be expected to be a reference material that is environmentally friendly. The results showed an increase in tensile strength of the composite fiber volume fraction of each additional up to 20%. At 10% fiber volume fraction values obtained 18.459N/mm2, then power up the volume fraction of 20% to reach a value 45.698N/mm2, but declining power back on composite with 30% fiber volume fraction is 32.891N/mm2. From microstructure observations made by SEM photo can be seen in the composite fiber distributionuneven in some parts of the composite fiber reinforcing material contained gaps, so that part hasthe tensile strength values are different.Keywords: composite, composite tensile strength, static tensile strength fibers, Sansevieriacylindrica.
KEKUATAN TARIK SERAT IJUK (ARENGA PINNATA MERR) Munandar, Imam; Savetlana, Shirley; Sugiyanto, .
Jurnal Ilmiah Teknik Mesin FEMA Vol. 1 No. 3 (2013)
Publisher : FT UNILA

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Serat ijuk merupakan serat alami yang ketersediaannya berlimpah, tetapi belum dimanfaatkan secara optimal. Serat ijuk dapat digunakan sebagai penguat alternatif untuk bahan komposit. Tujuan penelitian ini adalah untuk mengetahui kekuatan tarik dan morphologi serat ijuk melalui hasil pengamatan photo Scanning Electron Microscope (SEM).Pengekstrakan serat ijuk dilakukan dengan menggunakan sisir kawat yang berfungsi untuk memisahkan serat ijuk dengan pelepahnya. Dalam penelitian ini, serat ijuk yang dipilih yaitu berdiameter 0.25- 0.35mm, 0.36-0.45mm, dan 0.46-0.55 mm. Selanjutnya dilakukan perendaman  C selama 15 menit. Setelah itu dila kukan pengujian tarik dengan standar ASTM D 3379-75.Hasil dari penelitian didapatkan bahwa semakin kecil diameter serat, maka kekuatan tariknya semakin tinggi. Kekuatan tarik terbesar pada kelompok serat ijuk berdiameter kecil (0.25-0.35 mm) adalah  sebesar 208.22 MPa, regangan 0.192%, modulus elastisitas 5.37GPa dibandingkan kelompok serat ijuk dengan diameter besar (0.46-0.55 mm) sebesar 198.15 MPa, regangan 0.37%, modulus elastisitas 2.84 GPa. Hal ini dikarenakan rongga pada serat berdiameter 0.46-0.55 mm lebih besar dibandingkan serat berdiameter 0.25-0.35 mm menggunakan  larutan alkali yaitu  NaOH 5%  selama 2 jam., kemudian di oven dengan suhu 80Keywords : Serat Ijuk, Oven, NaOH, Kekuatan Tarik, Scanning Electron Microscope (SEM)
Co-Authors . Martinus, . . Tarkono, . . Zulhanif Ari Beni Santoso Dodi Marlin, Dodi Edy Meiyanto Efri Mahmuda, Efri Imam Munandar Imran Oktariawan, Imran M.Ikhsan Taufik, M.Ikhsan Nunuk Aries Nurulita Rahmat Iskandar Fajri, Rahmat Iskandar Ririn Sumiyani Shirley Savetlana, Shirley Sudibyo Martono Syamsul Arif Sarifudin, Syamsul Arif Teguh Sugiarto, Teguh