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All Journal Jurnal Biologi Udayana JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Syntax Literate: Jurnal Ilmiah Indonesia ANNALES BOGORIENSES Teknologi Indonesia Annales Bogorienses

NUNIK SULISTINAH

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Author-ID : 391157

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Other

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ISOLASI, SELEKSI, DAN KARAKTERISASI MIKROBA PENDEGRADASI ASETONITRIL DARI LIMBAH INDUSTRI Sunarko, Bambang; Adityarini, Adityarini; Tambunan, Usman Sumo F; Sulistinah, Nunik
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A number of microbes which could grow on acetonitrile were isolated and selected from industrial effluents and were studied to characterise the isolate which has the best degrading capability.Cultures were grown on mineral medium with microelements and acetonitrile was added as sole source of energy, carbon, and nitrogen.Isolate D5, identified as Corynebacteriumsp.,was able to grow on high concentration acetonitrile (up to 5 % v/v) and exhibited the highest specific growth rate (j).When Corynebacterium D5 grew on 2 % (v/v) acetonitrile,the doubling time was 6 hours 40 minutes,the specific growth rate (p) was 0.1 h and the acetonitrile decreasing rate was 3.99 mM/h.Increasing of acetonitrile concentration would extend the doubling time, decline the maximum growth and specific growth rate (i), and biomass production of Corynebacterium 05.The products of acetonitrile degradation by Corynebacterium D5 were acetamide, acetic acid, and ammonia.The maximum growth of Corynebacterium D5 showed when /3-aminopropionitrile was used as a substrate.

PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Sulistinah, Nunik; Sunarko, Bambang
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(j was 6 h 40 minutes, the specific growth rate (J) was 0,046 h the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.

Identification Of Degradation Pathway Of Vinylacetate Using Bacterial Isolate V2 And Characterization Of The Involved Enzymes Sunarko, Bambang; Sulistinah, Nunik; Nieder, Maria; Meyer, Ortwin
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/13

Vinyl acetate is a toxic substance, but has a high commercial value. In this study we show that vinyl acetate is subject to microbial degradation at rates of up to 6.38 and 1 mmol/h per g (dry weight) under aerobic and anaerobic conditions, respectively. It was hydrolyzed by bacterium V2 to ethano, acetaldehyde and acetate. The enzymes involved in the metabolism of vinylacetate were vinyl acetate esterase, aldehyde dehydrogenase, and alcohol dehydrogenase, which localized in the cytoplasmic fraction. The Km values of vinyl acetate esterase and alcohol dehydrogenase were 6.13 mM and 0.24 mM. respectively. Vinyl acetate esterase hydrolyzed the ester to acetate and vinyl alcohol. The Latter isomerized spontaneously to acetaldehyde and was then converted to acetate.The acetaldehyde was disproportionated into ethanol and acetate. The acetate was then converted to acetyl coenzyme A and oxidized through the tricarboxylic acid cycle and the glyoxylate bypass . Â

BIOTRANSFORMASI 3-SIANOPIRIDIN MENJADI NIKOTINAMIDA DENGAN SEL CORYNEBACTERIUM D5 SEBAGAI BIOKATALIS Sunarko, Bambang; Sulistinah, Nunik
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

A number of microbes isolated from industrial effl uents were studied to characterise their capability to convert 3-cyanopiridine. Isolate D5, identifi ed as Corynebacterium D5, was able to hydrolise 3-cyanopridin to nicotinamide, nicotinic acid, and ammonia catalyzed by nitrile-hydratase and amidase. The activity of nitrile-hydratase and amidase of Corynebacterium D5 were 0.364 U nad 0.200 U, respectively. The optimum hydrolisis of 3-cyanopiridine were occurred at 25oC and pH 7.0. Corynebacterium D5 exhibited the best growth (0.43 g cells dry weight/liter), when 0.5% (v/v) acetonitrile and 20 mM 3-cyanopiridine were used as sources of carbon, energy, and nitrogen.

RHODOCOCCUS PYRIDINIVORANS STRAIN TPIK, A NITRILE-DEGRADING BACTERIUM ISOLATED FROM TAILING POND OF ANTAM GOLD MINING INDUSTRY , PONGKOR Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
Teknologi Indonesia Vol 38, No 2 (2015)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v38i2.158

Nitriles are widely manufactured and extensively used by chemical industries as synthesis intermediates. Although these compounds are generally highly toxic, due to their cyano functional group, they can be used by some microorganisms as carbon and/or nitrogen sources. Nitrilase catalyzes the direct cleavage of nitriles to the corresponding acids and ammonia, where as nitrile-hydratase catalyzes the hydratation of nitriles to amides. Applications of these nitrile-converting enzymes are now increasingly recognized for the production of several pharmaceutically important compounds and fine chemicals. By virtue of their capability to eliminate highly toxic nitriles, the enzymes also play a significant role in protecting the environment. Accordingly, it is very important to find microorganisms that have a great capacity to utilize or degrade nitriles. Recently, a bacterial isolate TPIK which shows high nitrile degrading activity has been isolated. The strain was isolated from tailing pond of gold mine activity by selective enrichment methods. TPIK was shown to be capable of degrading high concentrations of nitrile (up to approx. 1 M acetonitrile). Colonies of TPIK are light orange in colour and have irregularly round wrinkles. The cells of TPIK are non-spore-forming, non motile and Gram-positive, but are Gram-variable in old cultures. The cells are rods and brached fillaments during the early growth phase and then fragmented into short rods and cocci. The taxonomic position of TPIK was clarified using molecular genetic methods. The phylogenetic tree showed that bacterial isolate TPIK falls within an evolutionary radiation comprising Rhodococcus species and is most closely related to the type strain of Rhodococcus pyridinivorans, sharing 99% 16S rDNA similarity. To the best of our knowledge, this was the first time that Rhodococcus pyridinivorans has been described as containing nitrile-degrading enzymes. Usually, this bacterial strain has been studied for its ability to degrade carcinogenic compounds, like pyridine.

KINETIKA BIOTRANSFORMASI SUKSINONITRIL OLEH Pseudomonas sp. Sulistinah, Nunik; Sunarko, Bambang
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Succinic acid (HOOC(CH2)2COOH) is commercial compound that primarily used in food products. This compound also used in textile industry for colouring process, drugs industry, paint, pernist and photography slice. Succinic acid usually produced in industry by chemical reaction and produce cyanide acid as by product. The objective of this research was to study the utilization of Pseudomonas sp. in produce succinic acid and to characterize of involved enzymes. The results showed that the enzyme optimally produce in log phase. The production rate of succinic acid formation was 0,982 mM/ml.h with the decrease of substrate consumption was 1,235 mM/ml.h. The yield of succinic acid during 81 hours fermentation was 81%. The optimum pH, temperature, Km and Vran were 7, 27°C, 90 mM, and 0,0002 mM/ml.h.

A NEW INDIGENOUS CYANOMETHANE-DEGRADING BACTERIUM ISOLATED FROM GOLD MINING WASTE WATER Sulistinah, Nunik; Munandar, Hendra; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3807

ABSTRACT The gold mining wastewater effluent is potential source for isolating cyanides-degrading bacteria, since cyanide is commonly used in gold extraction process in the mining industry. An indigenous bacterial strain LP3, capable of growing on and utilizing of a high concentration of cyanomethane (up to 1.0 Molar), could be isolated from Cikotok gold mine effluent. Based on 16S rDNA sequence, the strain was identified as Rhodococcus pyridinivorans. During the growth on cyanomethane (CH3CN), ethanamide (CH3ONH2) and ethanoic acid (CH3COOH) were detected in the growth media, indicating that nitrile hydratase and amidase involved in the metabolism of the substrate. The involvement of both enzymes on the conversion of cyanomethane was also proved by our study on cyanomethane biodegradation using whole cells of R. Pyridinivorans LP3. Besides cyanomethane, the R. pyridinivorans LP3 could also utilize various aliphatic, aromatic, heterocyclic nitriles and amides as growth substrates. Base on these results, R. pyridinivorans LP3 is expected to be used as a potential candidate for biological treatment for cyanide-containing wastes, although further research is still needed, before being applied on a field scale. Keywords: biocatalyst, cyanide degrading bacteria, gold mining, Rhodococcus pyridinivorans LP3

KONSORSIA BAKTERI PENGURAI SIANIDA YANG DI ISOLASI DARI BUANGAN INDUSTRI PENGOLAHAN EMAS Sulistinah, Nunik; Sunarko, Bambang
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Bacterial consortium capable of growing and utilizing cyanide as a source of nitrogenÃ‚Â were isolated from effluent of gold mining industry. The isolation was conducted usingÃ‚Â liquid enrichment medium with potassium cyanide and glucose as nitrogen and carbonÃ‚Â source, respectively. These consortium could tolerate and were able to grow on KCN atÃ‚Â concentration of up to 1000 ppm. Bacterial consortium LP3 were also able to degradeÃ‚Â potassium cyanide and ammonium as product of the degradation. The degradation rateÃ‚Â was 9,0ÃŽÂ¼M per minute. The cyanide-degrading bacteria found in this consortium wereÃ‚Â identified as Bacillus, Corynebacterium, and Serratia.Keywords: potassium cyanide, nitrogen source, bacterial consortium LP3,degradation

PENGARUH RESIDU PESTISIDA TERHADAP POLA POPULASI BAKTERI DAN FUNGI TANAH DI RUMAHKACA Sulistinah, Nunik; Antonius, S; Rahmansyah, Maman
Jurnal Teknologi Lingkungan Vol. 12 No. 1 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Pesticide Residue was Affect Bacterial and Fungal Population in the Greenhouse Soil Condition. Bacterial and fungal inhabitants were examined through agriculture soil samples. Survival of microorganism in soil was important to evaluate the mineralize process. In the greenhouse experiment, bacterial and fungal population noticed as essential assessment in soil healing with long lasting pesticide (A-soil) after the soil treated with powdered rice straw as organic matter amendment, toxic degrading bacterial as inoculants, and the both of those mixed treatments. That residue implication also assessed to B-soil (as free pesticide soil) then revised with pesticides, and also treated as followed for A-soil. Potential degradation of indigenous microorganism examined all through as setting of control (B-soil) to recognize of its original population.Those of treatments evaluated through microbial survival along 12 weeks incubation in green house environment. Bacterial and fungal populations was affect to become fluctuated along with incubation period as due to the treatments. Bacterial inhabitant was considerably higher compared to fungal population. Correlations were significantly difference with bacterial (p = 0.6654) as well as fungal (p = 0.9029) population in A-soil evaluated to B-soil habitats since the mixed treatments present to both of the soil (p0.005 = 0.6310). Organic matter alteration and certain microbe input was needed in soil contain pesticide, because at the same time the survival of microbes possibly mineralized organic matter, as well as the pesticide residue.

KEMAMPUAN Flavobacterium sp NUB1 DALAM MENGGUNAKAN ALIFATIK NITRIL UNTUK PERTUMBUHANNYA Sulistinah, Nunik
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

A bacteria isolate capable utilizing 1% (v/v) acetonitrile and butironitrile as thesolesource of carbon and nitrogen was isolated from industrial effluents ofPTPetrokimia-Gresik and identified as Flavobacterium sp NUB1. The bacteriaisolatewas able to grow in both acetonitrile and butironitrile at concentrationofup to 4% (v/v). The highest growth was reached at 1% concentration ofacetonitrileand butironitrile, but the bacteria isolate was not able to grow onacrilonitrile.The specific growth rate (ÃŽÂ¼) of the isolate was 0,029 h-1. Themajorobjective of this study was to explore the abilities of the isolate to utilizesomealiphatic nitriles and then further evaluate the metabolite product of thenitriledegradation.Key words: aliphatic nitrile, biodegradation, Flavobacterium sp. NUB1

Co-Authors Adityarini Adityarini Adityarini, Adityarini Afianisa, Salma Ahmad Thontowi Antonius, S Bambang Sunarko BAMBANG SUNARKO Endang Saepudin, Endang Kaban, Joseva Sudiati Kaban, Joseva Sudiati Maman Rahmansyah Meyer, Ortwin Meyer, Ortwin Munandar, Hendra Nieder, Maria Nieder, Maria Rini Riffiani Soenarko, Bambang Tambunan, Usman Sumo F Usman Sumo F Tambunan

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