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All Journal Jurnal Biologi Udayana JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Syntax Literate: Jurnal Ilmiah Indonesia ANNALES BOGORIENSES Teknologi Indonesia Annales Bogorienses
NUNIK SULISTINAH
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Other

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AKTIVITAS ENZIM 2,4-D MONOOKSIGENASE DARI BERBAGAI MIKROBA Nunik Sulistinah
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1242

Abstract

Nine cultures both from fungi and bacteria have been selected for testing their 2,4-D monooxygenase activity to degrade 2,4-Dtohlorophenoxyacetic acid (2,4-D). The results showed that all the cultures which were tested grows at 1000 ppm 2,4-D. Three cultures (Trichoderma viride. Asperoillus niqer and Isolat E (unidentified)) of the nine cultures are able to grow at 4000 ppm. T. viride grows well on Minimal Basal Media which contained glucose and 2000 ppm 2,4-D and produced the highest biomass (0.8660 g/l) than the others. The biomass of T. viride grew on MBM (without glucose) and added with 2000 ppm 2,4-D is 0,6520g/l. This indicated that the culture is tolerant to 2,4-D and able to use 2,4-D compound as energy and carbon sources for its growth. But we failed to prove the 2,4-D monooxygenase activity of supernatant of T. viride by measuring the changing of pH-value in the 2,4-D breakdown reaction.
PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Nunik Sulistinah; Bambang Sunarko
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1104

Abstract

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(\j was 6 h 40 minutes, the specific growth rate (\J) was 0,046 h'\ the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.
Biodegradation Potential of Cyanide and Nitrile using Bacteria of The Genus Rhodococcus Afianisa, Salma; Saepudin, Endang; Sunarko, Bambang; Sulistinah, Nunik
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v9i6.16689

Abstract

Cyanides and nitriles, characterized by their R-CN chains, are known for their toxicity, mutagenicity, and carcinogenicity, posing significant threats to environmental and human health. This study aims to explore the biodegradation capabilities of Rhodococcus sp. in breaking down cyanide and nitrile bonds. Rhodococcus pyridinivorans strain I-benzo was isolated from tanning waste and cultured in mineral media with a 20 mM benzonitrile substrate. The activity of this strain was tested using substrates such as benzonitrile, acetonitrile, acrylonitrile, benzamide, acetamide, and acrylamide, revealing positive reactions of nitrile hydratase and amidase enzymes through the Nessler measurement method, which indicated the production of ammonia and carboxylic acids. Furthermore, the degradation tests showed that the Vmax values for the biodegradation of potassium cyanide and sodium cyanide were 0.56 ppm/minute and 0.21 ppm/minute, respectively. These findings highlight the potential application of Rhodococcus pyridinivorans strain I-benzo in mitigating the environmental impact of cyanide and nitrile pollutants through efficient biodegradation.
Identification of Degradation Pathway of Vinyl Acetate Using Bacterial Isolate V2 and Characterization of The Involved Enzymes Soenarko, Bambang; Sulistinah, Nunik; Nieder, Maria; Meyer, Ortwin
Annales Bogorienses Vol. 10 No. 1 (2005): Annales Bogorienses
Publisher : BRIN

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Abstract

Vinyl acetate is a toxic substance, but has a high commercial value. In this study we show that vinyl acetate is subject to microbial degradation at rates of up to 6.38 and 1 mmol 1 per g (dry weight) under aerobic and anaerobic conditions, respectively. It was hydrolyzed by bacterium V2 to ethanol, acetaldehyde and acetate. The enzymes involved in the metabolism of vinyl acetate were vinyl acetate esterase, aldehyde dehydrogenase, and alcohol dehydrogenase, which localized in the cytoplasmic fraction. The Km values of vinyl acetate esterase and alcohol dehydrogenase were 6.13 mM and 0.24 mM. respectively. Vinyl acetate esterase hydrolyzed the ester to acetate and vinyl alcohol. The latter isomerized spontaneously to acetaldehyde and was then converted to acetate. The acetaldehyde was disproportionated into ethanol and acetate. The acetate was then converted to acetyl coenzyme A and oxidized through the tricarboxylic acid cycle and the glyoxylate bypass.
Co-Authors Adityarini Adityarini Adityarini, Adityarini Afianisa, Salma Ahmad Thontowi Antonius, S Bambang Sunarko BAMBANG SUNARKO Endang Saepudin, Endang Kaban, Joseva Sudiati Kaban, Joseva Sudiati Maman Rahmansyah Meyer, Ortwin Meyer, Ortwin Munandar, Hendra Nieder, Maria Nieder, Maria Rini Riffiani Soenarko, Bambang Tambunan, Usman Sumo F Usman Sumo F Tambunan