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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Innovative: Journal Of Social Science Research Jurnal Laboratorium Khatulistiwa Jurnal Info Kesehatan
Wahdaniah Wahdaniah
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Other

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Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit Wahdaniah Wahdaniah; Sri Tumpuk
Jurnal Laboratorium Khatulistiwa Vol 1, No 2 (2018): Mei 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i2.147

Abstract

Abstract: Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk  K2EDTA dan  K3EDTA. K3EDTA  biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.
Perbedaan Nilai Masa Pembekuan Darah (Clotting Time) dengan Menggunakan Tabung Kaca dan Tabung Plastik Metode Lee and White Ria Wulansari; Wahdaniah Wahdaniah; Suwono Suwono
Jurnal Laboratorium Khatulistiwa Vol 2, No 2 (2019): Mei 2019
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i2.333

Abstract

Abstract: The period of blood coagulation is the time needed to measure the duration of blood clots. Laboratory examination generally passes through 3 stages: preanalytic, analytic and post analytic stages. At the preanalytic stage should be considered such as the use of glass tubes and plastic tubes. As well as various technical errors tend to shorten or extend the freezing period. This study aims to determine whether there is any difference in the value of clotting time by using glass and plastic tube of Lee and White method. The research method used is Quasi Experiment Design. The sampling technique uses total sampling. The sample in this study is a D-IV level I B Health Analyst who amounted to 47 people. Based on data analysis using Wilcoxon test, the signifcant value of P = 0.000 (P <0,05) then Ho is rejected and Ha accepted which indicate that there is difference of clotting time between glass tube with plastic tube of Lee and White method.Abstrak: Masa pembekuan darah adalah waktu yang diperlukan untuk mengukur lamanya darah membeku. Pemeriksaan laboratorium pada umumnya melewati 3 tahap yaitu tahap praanalitik, analitik dan pasca analitik. Pada tahap pra analitik harus diperhatikan seperti penggunaan tabung kaca dan tabung plastik. Serta bermacam-macam kesalahan teknik cenderung memperpendek atau memperpanjang masa pembekuan. Penelitian ini bertujuan untuk mengetahui ada tidaknya perbedaan nilai masa pembekuan darah (clotting time) dengan menggunakan tabung kaca dan tabung plastik metode Lee and White. Metode penelitian yang digunakan adalah Quasi Experiment Design. Teknik pengambilan sampel menggunakan total sampling. Sampel pada penelitian ini adalah mahasiswa D-IV tingkat I B Analis Kesehatan yang berjumlah 47 orang. Berdasarkan Analisis data menggunakan uji Wilcoxon diperoleh nilai Signifkan P = 0,000 (P< 0,05) maka Ho ditolak dan Ha diterima yang menunjukkan bahwa terdapat perbedaan masa pembekuan darah (clotting time) antara tabung kaca dengan tabung plastik metode Lee and White.
Hubungan Jumlah Trombosit dengan Nilai Prothrombin Time dan Activated Partial Thromboplastin Time pada Pasien Persiapan Tindakan Operasi Caesar Wahdaniah Wahdaniah; Sri Tumpuk
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.88

Abstract

Abstract: Sometimes in postpartum haemorrhage, there is happen great haemorrhage which is blood platelets (thrombocyte) have an important role in the process of hemostasis. Prothrombin (PT) assays are useful for assessing the ability of coagulation factors of extrinsic pathways and joint pathways. The period of activated partial thromboplastin (aPTT) is a laboratory test for assessing coagulation abnormalities in intrinsic pathways and joint pathways. This study was aimed to analyze the correlation of platelet counts with PT and aPTT values in patients preparing for caesarean section in Public Hospital of St. Antonius Pontianak. This study used a cross-sectional design with a sample of all pregnant women who performed a caesarean section preparation of 43 people. Based on data analysis using Spearman test on PT variable obtained p-value = 0.059 (p > 0,05) indicating that there is no relation between platelet count with value of PT and at variable aPTT obtained value p = 0,737 (p > 0,05) there is no correlation between platelet count and aPTT value.Abstrak: Perdarahan pasca melahirkan ada kalanya terjadi perdarahan yang hebat dimana trombosit berperan penting dalam proses homeostatis. Uji masa protrombin (PT) berguna untuk menilai kemampuan faktor koagulasi jalur ekstrinsik dan jalur bersama. Masa tromboplastin parsial teraktivasi (aPTT) adalah uji laboratorium untuk menilai kelainan koagulasi pada jalur intrinsik dan jalur bersama. Penelitian ini bertujuan untuk menganalisis hubungan jumlah trombosit dengan nilai PT dan aPTT pada pasien persiapan tindakan operasi caesar di RSU. St. Antonius Pontianak. Penelitian ini menggunakan desain cross sectional dengan sampel semua pasien ibu hamil yang melakukan persiapan tindakan operasi caesar sebanyak 43 orang. Berdasarkan analisis data menggunakan uji Spearman pada variabel PT diperoleh nilai p = 0.059 (p > 0,05) yang menunjukkan bahwa tidak ada hubungan antara jumlah trombosit dengan nilai PT dan pada variabel aPTT diperoleh nilai p = 0.737 (p > 0.05) yang menunjukkan bahwa tidak ada hubungan antara jumlah trombosit dengan nilai aPTT.
Perbedaan Profil Darah pada Mahasiswa Perokok dengan Bukan Perokok Di Jurusan Analis Kesehatan Pontianak Lusia Rosauli Stefani; Sri Tumpuk; Wahdaniah Wahdaniah
Jurnal Laboratorium Khatulistiwa Vol 2, No 1 (2018): November 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i1.321

Abstract

Abstract: Cigarettes are one tobacco product intended to be burned and smoked, the smoke containing nicotine, tar and carbon monoxide. Carbon monoxide has a strong tendency to bind to hemoglobin in red blood cells and increase the viscosity of blood making it easier for blood clots. Cigarettes cause the imbalance of antioxidants in the body characterized by the stimulation of the hematopoietic system, especially the bone marrow in producing and excluding leukocytes in the circulation including the activated neutrophils. The purpose of this research was to determine the difference of blood profles in non smoker students in Pontianak Health Analyst Department. The research was Cross-sectional and sampling technique used was total sampling. The sample in this study were D-IV Health Analysts level 1 to 4 students who were 25 smokers and 25 non-smokers. The blood profles were examined by using the analytical analyzer. The results showed the difference of hemoglobin, hematocrit and red blood cell count with sig <0.05 whereas the white blood cell count and neutrophil type did not show any difference with the sig value> 0.05 on 25 respondents smokers and 25 respondents are not smokers.Abstrak: Rokok adalah salah satu produk tembakau yang dimaksudkan untuk dibakar dan dihisap, asapnya mengandung nikotin, tar dan karbon monoksida. Karbon monoksida memiliki kecenderungan yang kuat untuk berikatan dengan hemoglobin dalam sel darah merah dan meningkatkan viskositas darah sehingga mempermudah penggumpalan darah. Rokok menyebabkan ketidakseimbangan antioksidan dalam tubuh ditandai oleh stimulasi dari sistem hematopoietik, khususnya sumsum tulang dalam menghasilkan dan mengeluarkan leukosit pada sirkulasi termasuk neutrofl yang teraktivasi. Penelitian ini bertujuan untuk mengetahui perbedaan profl darah pada mahasiswa perokok dengan bukan perokok di Jurusan Analis Kesehatan Pontianak. Penelitian ini adalah penelitian Cross-sectional dan teknik sampling yang digunakan adalah total sampling. Sampel pada penelitian ini adalah mahasiswa D-IV Analis Kesehatan tingkat 1 sampai 4 yang berjumlah 25 orang perokok dan 25 orang bukan perokok. Profl darah responden diperiksa secara otomatis menggunakan alat hematology analyzer. Hasil penelitian menunjukan adanya perbedaan kadar hemoglobin, hematokrit dan hitung jumah sel darah merah dengan nilai p< 0.05 sedangkan hasil hitung jumlah sel darah putih dan jenis neutrofl tidak menunjukan adanya perbedaan dengan nilai p>0.05 pada 25 responden perokok dan 25 responden bukan perokok.
Pengaruh Latihan Fisik Lari Jarak Pendek terhadap Masa Pendarahan (Bleeding Time) Metode IVY Sri Tumpuk; Wahdaniah Wahdaniah
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.96

Abstract

Abstract: Running is very important to maintain and improve the physical quality of human resources. By doing physical exercise can reduce the risk factor for the occurrence of interference with the mechanism of hemostasis. One of the examination filters to see the abnormalities in the process of hemostasis is bleeding time. The purpose of this study was to determine the bleeding time before and after physical exercise (sprint), to determine the effect of physical exercise (sprint) to the bleeding time before and after physical exercise (sprint) done for 5 minutes. The research design used was Cross-Sectional and the sampling technique used was total population. The sample in this study were all students D-III 2nd grade of Medical Laboratory class of 2015 which amounted to 55 people. Examination of this bleeding time using Ivy method. Furthermore, the data were analyzed by simple linear regression test. Based on the results of the study, the average number before exercises was ±118 seconds (117.82 seconds), and after physical exercise (sprint) was ±161 seconds (160.91 seconds) with the difference between them was 43 seconds (43.09 seconds). Statistically obtained p = 0,000 (p <0,05) or there was significant influence between physical exercise (sprint) to bleeding time.Abstrak: Latihan lari sangat penting untuk menjaga dan meningkatkan kualitas fsik sumber daya manusia. Dengan melakukan latihan fsik dapat menurunkan faktor risiko terjadinya gangguan pada mekanisme homeostasis. Salah satu pemeriksaan penyaring untuk melihat kelainan pada proses homeostasis adalah masa perdarahan. Tujuan dari penelitian ini adalah untuk mengetahui masa perdarahan sebelum dan sesudah latihan fsik lari jarak pendek, mengetahui pengaruh latihan fsik lari terhadap masa perdarahan sebelum dan sesudah mthe elakukan latihan fsik lari selama 5 menit. Desain penelitian yang digunakan adalah Cross sectional dan teknik sampling yang digunakan adalah total populasi. Sampel pada penelitian ini adalah seluruh mahasiswa/I D-III tingkat 2 Analis Kesehatan angkatan tahun 2015 yang berjumlah 55 orang. Pemeriksaan masa perdarahan ini menggunakan metode Ivy. Selanjutnya data dianalisis dengan uji regresi linier sederhana. Berdasarkan hasil penelitian didapatkan jumlah rata-rata sebelum melakukan latihan ± 118 detik (117,82 detik), dan setelah melakukan latihan fsik lari adalah ± 161 detik (160,91 detik) dengan selisih diantara keduanya sebesar 43 detik (43,09 detik). Secara statistik diperoleh nilai p=0,000 (p < 0,05) atau ada pengaruh yang signifkan antara latihan fsik lari terhadap masa perdarahan
Profil Resistensi Bakteri Patogen Pada Minuman Air Tahu Di Wilayah Kota Pontianak Talen Nikita Sianturi; Wahdaniah Wahdaniah; Sugito Sugito; Bagus Muhammad Ihsan
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v3i6.6780

Abstract

Minuman air tahu adalah minuman yang dibuat dari kacang kedelai kuning, minuman ini berwarna putih kekuningan mirip dengan susu. Minuman ini banyak diminati oleh masyarakat Pontianak karena rasanya yang nikmat dan harganya yang terjangkau. Minuman tersebut dapat tercemar oleh bakteri patogen karena higiene penjual dan lingkungan yang kurang baik. Cemaran oleh minuman bakteri patogen yang resisten terhadap antibiotik merupakan ancaman kesehatan yang serius. Penelitian ini bertujuan untuk mengidentifikasi dan memberikan gambaran resistensi antibiotik yang diisolasi dari minuman air tahu. Penelitian berbentuk deskripsi observasi dengan metode kultur dan uji resistensi metode kirby-bauer. Antibiotik yang digunakan adalah penisilin, amoksilin, siprofloksasin, dan kloramfenikol. Jumlah sampel sebanyak 43 sampel. Hasil identifikasi ditemukan bakteri Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, dan Klebsiella pneumoniae. Hasil uji resitensi menunjukkan Staphylococcus aureus resisten terhadap penisilin (100%), Salmonella typhi resisten terhadap penisilin (66,6%), Enterobacter aerogenes resisten terhadap kloramfenikol (40%), dan Klebsiella pneumoniae resisten terhadap penisilin (100%).
The Effect of Bay Leaf (Syzygium Polyanthum) Extract Hand Washing Liquid Soap Preparation on The Growth of Escherichia Coli Bacteria in Vitro Wahdaniah; Asep Sabolakna; Bella Ramadhanti; Bagus Muhammad Ihsan; Yeremiah Rubin Tjamin
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7175

Abstract

Bay leaf (Syzygium polyanthum) is known as a complement to cooking spices, also used by the community for treatment. The content of antibacterial compounds from bay leaves such as alkaloids, flavonoids, saponins and tannins which can inhibit bacterial growth. This study aims to determine the effect of hand washing liquid soap with bay leaf extract on the growth of Escherichia coli bacteria. This study uses a Quasi Experiment research design. The population in this study is located on Jalan Atot Ahmad, West Pontianak District. The sample used was liquid hand washing soap with bay leaf extract at a concentration of 3, 6, and 9%, 9 repetitions with a total of 27 samples, using the disc diffusion method. The results of the antibacterial study showed that the average diameter of the inhibition zone at 3% concentration was 8.27 mm, 6% concentration was 10.55 mm and 9% concentration was 11.16. The results of the Linear Regression test using the Anova test obtained pvalue = 0.000, which is pvalue < α, with α = 0.05, so that it was stated that H1 was accepted so that there was an effect on the preparation of liquid hand washing soap (Syzygium polyanthum) extract on the growth of Escherichia coli bacteria in vitro.
The Effectiveness of Pineapple Extract (Ananas Comosus) and Kesum Leaves (Polygonus Minus) on the Quality of Coconut Oil (Coconus Nucifera) Hendra Budi Sungkawa; Wahdaniah Wahdaniah; Herlinda Djohan
JURNAL INFO KESEHATAN Vol 19 No 2 (2021): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol19.Iss2.519

Abstract

The processed oil from the coconut plant is generally understood as coconut oil. A method is required to produce a product with a higher oil extraction rate and is able to reduce the water content and free fatty acids in the coconut oil production. It is also necessary to add substances that can delay or prevent fat oxidation reactions by generating substances in the form of antioxidants. The method that can be implemented is the enzymatic method employing the bromelain enzyme in a pineapple with the addition of an antioxidants substance from the kesum leaf. The objective of this research is to describe the quality of coconut oil after the addition of pineapple (ananas comosus) and kesum leaves (polygonus minus) extracts. The parameters for describing the quality of the oil are the organoleptic test, the degree of acidity, the oil extract rate, the peroxide number, the saponification number, and the acid number. This research is a quasi-experiment. The samples in this research were coconut oil without the addition of pineapple fruit extract, coconut oil with the addition of pineapple fruit extract without the addition of kesum leaves, and coconut oil with the addition of pineapple fruit extract and kesum leaves as much as 20gr, 30gr and 40gr. Based on the statistical results of the linear regression test, it was discovered that p-value = 0.000 <0.05, so it was concluded that there was an effect of the addition of pineapple fruit and leaves of kesum on acid number content with an effect of 76.4% on the acid number, 71.4% on the peroxide number, and 81.5% to the saponification number. It is recommended to test the water content, free fatty acids, and iodine number.
Co-Authors Asep Sabolakna Asep Sabolakna Bagus Muhammad Ihsan Bella Ramadhanti Emilda Sari Emilda Sari Etiek Nurhayati Fitria Margini Hendra Budi Sungkawa Herlinda Djohan Indah Purwaningsih Khaulani Refsi Hardini Kurnia Herfani Lusia Rosauli Stefani Mega Erika Natasya Intan Ramadhani Ria Wulansari Sherin Aulia Sukma Sri Tumpuk Sri Tumpuk Sugito Sugito Suwandi, Edy Suwono Suwono Talen Nikita Sianturi Yeremiah Rubin Tjamin