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Potensi Enzim Bromelin Sari Buah Nanas (ananas comosus l.) Dalam Meningkatkan Kadar Protein Pada Tahu Purwaningsih, Indah
Jurnal Teknologi Laboratorium Vol 6 No 1 (2017): 2017 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.184 KB) | DOI: 10.29238/teknolabjournal.v6i1.86

Abstract

Tofu is one of processed soybeans that have a high nutritional value and low price. Levels of protein in tofu can be enhanced by enzymatically. One of the protease enzyme which is commonly used in the food industry is bromelain enzyme. The bromelain enzyme in pineapple is widely available. The purpose of this study was to determine the effect of the bromelain enzyme from pineapple juice to protein content by examining the enzyme concentration and incubation time to get the best protein content.This research was quation experiment. The experimental design for this research was randomized block design with 2 factors. Factor I consist of 4 levels (enzyme concentration 35%, 40%, 45% and 50%) and factor II consist of 2 levels (incubation period with enzyme of 6 and 12 hours). The data obtained were then analyzed statistically by multiple linear regression test.The results showed that the best protein content was obtained from the preparation of tofu with the addition of bromelin enzyme from pineapple juice with concentration of 50% and with the incubation time for 12 hours, that is 16,6195%. Statistical test results obtained value of p <0.05, so it can be concluded that there were a relationship/correlation between the enzyme concentration and incubation time to the protein content. Based on the results of research can be suggested that bromelin enzyme from pineapple juice can be used to increase protein content in tofu.
Penyuluhan Kesehatan dan Pelatihan Pembuatan Pasta Gigi Herbal pada Orang Tua Siswa di SDN 09 Pontianak Fathiah Fathiah; Indah Purwaningsih; Sunarsieh Sunarsieh; Budi Suryana; Meri Ropiqa
Poltekita: Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2023): Januari-Maret
Publisher : Pusat Penelitian & Pengabdian Masyarakat Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.01 KB) | DOI: 10.33860/pjpm.v4i1.1541

Abstract

Riskesdas data in 2018 showed that the number of tooth decays in West Kalimantan province was 49.55% and that in Pontianak City was 39.52%. The results of a preliminary survey conducted on 5th-grade students of SDN 09 Pontianak found an average of three deciduous teeth and four adult teeth affected by dental caries. One way to prevent dental caries is to brush the teeth using toothpaste that contain active antibacterial ingredients. One herb widely used as an active ingredient in toothpaste is siwak. This community service activity aims to increase knowledge and skills in making herbal toothpaste to reduce the dental plaque index. This activity uses health counseling methods through lectures, leaflet distribution, and training on making herbal toothpaste from siwak. This activity was conducted in collaboration with SDN 09 Pontianak and was held on Friday, June 24, 2022, with 53 participants. From this activity, the participants received education on the benefits of herbal toothpaste for dental and oral health. In addition, this training activity can increase creativity and skills in creating herbal toothpaste and foster an entrepreneurial spirit. After this activity, it will continue with training activities with the theme of entrepreneurship as part of efforts to downstream and commercialize herbal toothpaste products.
Perbandingan Kadar Bilangan Asam Minyak Goreng Sawit Curah yang Ditambahkan Ekstrak Wortel dengan Yang Tidak Indah Purwaningsih
Jurnal Vokasi Kesehatan Vol 1, No 2 (2015): Maret 2015
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.59 KB) | DOI: 10.30602/jvk.v1i2.15

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Abstract: The Comparison Levels of Palm Oil Acid Numbers Which Added Carrot Extract With Not Added. The aims of this study were to determine the differences of carrot extract as the natural antioxidant to levels of palm oil acid numbers which experiencing 1, 2, 3 and 4 times of heating for 5 minutes at temperature 100 °C. The design of this study was the quasi experiment and the sampling technique was simple random sampling. Based on Two Way ANOVA test, obtained test results significantly (p-value <0.05) at 95% confidence level was which on heating significant value 0,000 which means that heating had significant effect on the levels of acid number and which on beta-carotene significant value of 0.000 which means the addition of beta-carotene had significant effect on  levels of acid number. So, we can conclude that Ha was accepted, which means that there were difference levels of palm oil acid numbers which added carrot extract with not added carrots extract which experiencing 1, 2, 3 and 4 times of heating.Abstrak : Perbandingan Kadar Bilangan Asam Minyak Goreng Sawit Curah Yang Ditambahkan Ekstrak Wortel Dengan Yang Tidak. Penelitian ini bertujuan untuk mengetahui perbedaan pemberian ekstrak wortel sebagai antioksidan alami terhadap kadar bilangan asam minyak goreng sawit curah yang telah mengalami 1, 2, 3 dan 4 kali pemanasan selama 5 menit pada suhu 100°C. Desain penelitian yang  digunakan adalah eksperimen kuasi dengan teknik pengambilan sampel yang digunakan adalah Simple Random Sampling. Berdasarkan uji Two Way Anova, diperoleh hasil uji signifikan (p value < 0,05) pada tingkat kepercayaan 95% yaitu pada penggorengan dengan hasil signifikan 0,001 sedangkan pada betakaroten dengan hasil signifikan 0,002 maka dapat disimpulkan ada perbedaan kadar bilangan asam minyak goreng sawit curah yang ditambahkan ekstrak wortel dengan yang tidak ditambahkan ekstrak wortel yang telah mengalami 1, 2, 3 dan 4 kali pemanasan.
Perbandingan Perendaman Asam Sitrat dan Jeruk Nipis terhadap Penurunan Kadar Kalsium Oksalat pada Talas Indah Purwaningsih; Kuswiyanto Kuswiyanto
Jurnal Vokasi Kesehatan Vol 2, No 1 (2016): Januari 2016
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.294 KB) | DOI: 10.30602/jvk.v2i1.61

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Abstract : Comparative Using Citric Acid Immersion And Lime To Decrease The Level Of Calcium Oxalic Taro. The aim of this study is to reduce the levels of calcium oxalate in taro by using a solution of citric acid and lemon juice concentration of 1%, 5%, and 10%. The level of calcium oxalate in this study using titration methods permanganometry The results show that the effective concentration in the lower levels of calcium oxalate in the taro were the solution of citric acid concentration of 5%, which could reduce levels of calcium oxalate up to 41.7456% compared to baseline values and lime juice concentration of 5%, whereas levels of calcium oxalate decreased by 47, 6668% compared to baseline values.Abstrak : Perbandingan Perendaman Asam Sitrat Dan Jeruk Nipis Terhadap Penurunan Kadar Kalsium Oksalat Pada Talas. Penelitian ini bertujuan untuk mengurangi kadar kalsium oksalat pada talas dengan menggunakan larutan asam sitrat dan air perasan jeruk nipis dengan konsentrasi 1%, 5% dan 10%. Pemeriksaan kadar kalsium oksalat menggunakan metode titrasi permanganometri. hasil penelitian menunjukkan konsentrasi yang efektif dalam menurunkan kadar kalsium oksalat pada talas adalah larutan asam sitrat konsentrasi 5%, dimana mampu menurunkan kadar kalsium oksalat hingga 41,7456% dibandingkan kadar awal dan air perasan jeruk nipis konsentrasi 5%, dimana kadar kalsium oksalat mengalami penurunan sebesar 47,6668% dibandingkan kadar awal.
Aktivitas Antioksidan Ekstrak Rimpang Temu Ireng (Curcuma aeruginosa Roxb.) Metode DPPH Fadli Sukandiarsyah; Indah Purwaningsih; Gervacia Jenny Ratnawaty
Jurnal Mandala Pharmacon Indonesia Vol. 9 No. 1 (2023): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v9i1.299

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Temu ireng (Curcuma aeruginosa Roxb.) adalah tanaman obat tradisional yang mengandung senyawa fenol, steroid, triterpenoid, flavonoid, alkaloid, tanin, saponin. Penelitian ini menggunakan pelarut polar yaitu metanol dan nonpolar yaitu n-heksana untuk menarik senyawa sesuai dengan sifatnya. Tujuan penelitian ini untuk melihat aktivitas antioksidan ekstrak metanol dan ekstrak n-heksana rimpang temu ireng. Proses ekstraksi dilakukan dengan cara maserasi. Metode yang digunakan adalah metode DPPH. Sampel yang digunakan adalah ekstrak metanol rimpang temu ireng variasi konsentrasi 100, 150, 200, 250 dan 300 ppm serta ekstrak n-heksana rimpang temu ireng variasi konsentrasi 1500, 1600, 1700, 1800, 1900 ppm dengan pengulangan sebanyak tiga kali. Hasil penelitian diperoleh IC50 ekstrak metanol rimpang temu ireng sebesar 171 ppm, IC50 ekstrak n-heksana rimpang temu ireng sebesar 1.724 ppm. Pembanding yang digunakan adalah vitamin C dengan IC50 6,175 ppm. Disimpulkan bahwa ekstrak metanol rimpang temu ireng mempunyai aktivitas antioksidan lebih baik dari ekstrak n-heksana rimpang temu ireng.
Pengaruh Suhu Penyeduhan terhadap Kadar Protein pada Susu Formula Menggunakan Metode KJELDAHL Dian Kartika Ningrum; Gervacia Jenny Ratnawati; Indah Purwaningsih
Jurnal Laboratorium Khatulistiwa Vol 2, No 1 (2018): November 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i1.317

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Abstract: Formula milk is a liquid or powder with a specifc formula given to infants and children. Serves as a substitute for breast milk. Formula milk has an important role in baby food because it often acts as the only source of nutrition for infants. Milk includes a high quality protein source. The purpose of this research is to determine the effect of temperature brewing on protein content in formula milk using the KJELDAHL method. This research was an experimental research using quasi-experiment. The samples in this research were 25 samples consisting of 5 treatments with repetition of each treatment 5 times. The examination method used in this research is a Kjeldahl method. From these results, the data obtained were analyzed statistically using a simple linear regression test. The results obtained in this study were the mean protein content of formula milk that was brewed at 40˚C, 50˚C, 60˚C, 70˚C and 80˚C respectively were 5,38%, 5,52%, 5.82%, 5.62% and 5.43%. Based on the results of statistical tests obtain p-value (0.139)> α (0,05) which means that Ha is rejected. Of the result it can be concluded that there is no effect of temperature brewing on protein content in formula milk using KJELDAHL method.Abstrak: Susu formula adalah cairan atau bubuk dengan formula tertentu yang diberikan pada bayi dan anak-anak. Berfungsi sebagai pengganti ASI. Susu formula memiliki peranan yang penting dalam makanan bayi karena seringkali bertindak sebagai satu-satunya sumber gizi bagi bayi. Susu termasuk sumber protein berkualitas tinggi. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh suhu penyeduhan terhadap kadar protein pada susu formula dengan menggunakan metode kjeldahl. Penelitian ini merupakan penelitian eksperimental menggunakan quasi experiment. Sampel pada penelitian ini berjumlah 25 sampel yang terdiri dari 5 perlakuan dengan pengulangan setiap perlakuan sebanyak 5 kali. Metode pemeriksaan yang digunakan dalam penelitian ini yaitu metode KJELDAHL. Dari hasil tersebut, data yang diperoleh dianalisis secara statistik menggunakan uji regresi linear sederhana. Hasil yang diperoleh pada penelitian ini yaitu rata-rata kadar protein pada susu formula yang diseduh pada suhu 40˚C, 50˚C, 60˚C, 70˚C dan  0˚C berturut-turut adalah 5,38%, 5,52%, 5,82%, 5,62% dan 5,43%. Berdasarkan hasil uji statistik diperoleh nilai p (0,798) > α (0,05) yang berarti bahwa Ha ditolak. Dari hasil tersebut disimpulkan bahwa tidak ada pengaruh suhu penyeduhan terhadap kadar protein pada susu formula menggunakan metode KJELDAHL.
Personal Hygiene terhadap Infeksi Pityriasis Versikolor pada Nelayan Di Desa Penjajap Kecamatan Pemangkat Supriyanto Supriyanto; Indah Purwaningsih
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.99

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Abstract: Pityriasis versicolor or better known as “panu” is a superfcial fungal infection characterized by changes in skin pigment due to Stratum corneum colonization by dimorphic lipophilic fungi from normal skin flora. Pitiriasis versicolor is an infectious disease that is estimated occur due to poor sanitation (personal hygine) and lack of clean water. This research was aimed to determine factors related to Pityriasis versicolor infection. It used retrospective design where researcher tried to looking back about the incident of Pitiriasis versicolor on 76 fshermen who choosen by using simple random sampling. Based on the result of reseach, it was determine that bath habit (p = 0,000), clothing hygiene (p = 0,839), towels cleanliness (p = 0,699), clean water supply (p = 0,000), home environment hygiene (p = 0,588), for p<0,05 then these factors were related to the occurrence of Pitiriasis versicolor infection on fshermen in Penjajap Village Pemangkat. Thus, it could be conclude that there was signifcant correlation between bath habit and clean water supply with the incidence of Pityriasis versicolor infection. While the cleanliness of clothing, cleanliness of towels, and cleanliness of the home environment is not associated with the incidence of Pityriasis versicolor infection. Abstrak: Pityriasis versikolor atau lebih dikenal dengan panu adalah infeksi jamur superfsial yang ditandai perubahan pigmen kulit akibat kolonisasi stratum korneum oleh jamur lipoflik dimorfk dari flora normal kulit. Pityriasis versikolor merupakan penyakit menular yang diperkirakan terjadi karena sanitasi (personal hygiene) yang buruk dan kurangnya air bersih. Penelitian ini bertujuan untuk mengetahui hubungan faktor-faktor personal hygiene terhadap infeksi pityriasis versikolor. Penelitian ini menggunakan rancangan retrospektif dimana peneliti berusaha melihat ke belakang (backward looking) terhadap kejadian pityriasis versikolor pada 76 nelayan yang terpilih sebagai responden dengan teknik simple random sampling. Berdasarkan hasil penelitian diketahui bahwa kebiasaan mandi (p = 0,000), kebersihan pakaian (p = 0,839), kebersihan handuk (p = 0,699), persediaan air bersih (p = 0,000), kebersihan lingkungan rumah (p = 0,588), untuk p < 0,05 maka faktor-faktor tersebut berhubungan terhadap terjadinya infeksi pityriasis versikolor pada nelayan di Desa Penjajap Kecamatan Pemangkat. Dengan demikian dapat disimpulkan bahwa ada hubungan yang signifkan antara kebiasaan mandi dan persediaan air bersih dengan kejadian infeksi pityriasis versikolor. Sedangkan kebersihan pakaian, kebersihan handuk, dan kebersihan lingkungan rumah tidak berhubungan dengan kejadian infeksi pityriasis versikolor.
Toksisitas Akut Ekstrak Metanol Mentimun (Cucumis Sativus L.) Terhadap Larva Artemia Salina Leach Metode Brine Shrimp Lethality Test (BSLT) Khairin Akbar Putra; Indah Purwaningsih; Kuswiyanto Kuswiyanto
Jurnal Laboratorium Khatulistiwa Vol 2, No 2 (2019): Mei 2019
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i2.334

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Abstract: Cucumber (Cucumis sativus L) is a plant that is widely consumed by the people. Beside due the delicious taste of it, cucumbers also contain some compounds that can be used as a drug, one of them is to prevent cancer. This plant contains Flavonoid, Saponin, and Tanin. These three compounds are the compounds which is in certain levels can be cytotoxic. Acute toxicity is an early screening test for a specifc cytotoxic potential crop for the development of anti-cancer drugs. The purpose of this research is to know the acute toxicity of cucumber (Cucumis sativus L) methanol extract to the Artemia salina Leach larvae by Brine Shrimp Lethality Test (BSLT) method. This research method was an experimental by using BSLT method. The test animal used was the Artemia salina Leach larvae. The method of extraction was maseration method. The results of the study can be seen from the percentage of larvae mortality for each concentration of extract, those are 0 μg / ml, 250 μg / ml, 500 μg / ml, 750 μg / ml, 1000 μg / ml are 0%, 48%, 62 %, 72%, 84% and 92%. Based on the result of probit analysis with microsoft Excel showed that LC50 value of cucumber methanol extract is 201,0165 μg /ml which means this extract have potency of acute toxicity because LC50 value <1000 μg / ml.Abstrak: Mentimun (Cucumis sativus L) adalah tanaman yang banyak dikonsumsi oleh masyarakat. Selain rasanya yang enak, mentimun juga memiliki kandungan senyawa yang dapat bermanfaat sebagai obat, salah satunya adalah untuk mencegah terjadinya kanker. Tanaman ini mengandung Flavonoid, Saponin, dan Tanin. Ketiga senyawa ini merupakan senyawa yang dengan kadar tertentu dapat bersifat sitotoksik. Toksisitas akut merupakan skrining awal untuk menguji suatu tanaman tertentu yang memiliki potensi sitotoksik untuk pengembangan obat anti kanker. Tujuan penelitian ini untuk mengetahui toksisitas akut dari ekstrak metanol mentimun (Cucumis sativus L) terhadap larva Artemia salina Leach dengan metode Brine Shrimp Lethality Test (BSLT). Metode penelitian ini berbentuk eksperimental dengan menggunakan metode BSLT. Hewan uji yang digunakan adalah larva Artemia salina Leach. Metode ekstraksi yang digunakan adalah metode maserasi. Hasil penelitian dapat dilihat dari persentase kematian larva untuk setiap konsentrasi ekstrak, yaitu 0 µg/ml, 250 µg/ml, 500 µg/ml, 750 µg/ml, 1000 µg/ml secara berturut-turut adalah 0%, 48%, 62%, 72%, 84% dan 92%. Berdasarkan hasil analisis probit dengan microsoft Excel menunjukkan nilai LC50 ekstrak metanol mentimun adalah 201, 0165 µg/ml yang berarti ekstrak ini memiliki potensi toksisitas akut karena nilai LC50 < 1000 µg/ml.
Gambaran Protein Urin Dan Glukosa Urin Pada Penderita Diabetes Melitus Tipe II Persadia RSU Santo Antonius Pontianak Etiek Nurhayati; Indah Purwaningsih
Jurnal Laboratorium Khatulistiwa Vol 1, No 2 (2018): Mei 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i2.145

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Abstract: Diabetic mellitus  is a metabolic disease, with type I and II. Diabetic mellitus is chronic and  caused many organs complication. Diabetic nephrophathy is one of the manifestation of kidney complicattion that can caused the end stage of renal disease, and would need dyalisys therapy. The simple laboratory examination are urinay glucose test and protein urin test.The aim of this study  to determine the purpose  proteinuria and glucose urine of diabetic mellitus patient in Persadia of RSU St. Antonius. This  is a cross sectional study, with purposive sampling.  The proteinuria test and urine glucose by strip rapid test. This study was on Mei-November 2016. The 40 diabetic mellitus patient had participated. This study found 8  (20%) urin samples were protein urine positive  1, and  32 were negative. Urine glucose examination results 31 people negative, positive 1 in 5 people and positive 4 on 4 people. In type II DM patients found proteinuria and glucose in the urine, so patients with positive proteinuria should check again in 3-6 months to monitor kidney functionAbstrak: Diabetes mellitus (DM) adalah penyakit metabolik, yang terdiri atas tipe I dan II. Penyakit DM adalah penyakit kronik dengan banyak komplikasi. Komplikasi pada ginjal berupa nefropati diabetik merupakan salah satu penyebab end stage of renal disease, dan memerlukan terapi dialysis. Pemeriksaan yang sederhana dan cukup efektif untuk mengetahui fungsi ginjal adalah pemeriksaan glukosa urin dan protein urin.Penelitian ini bertujuan untuk mengetahui gambaran protein urin dan glukosa urin pada penderita DM tipe II di Persadia RSU St.Antonius Pontianak. Penelitian ini adalah cross sectional dan sampel diambil secara purposive sampling dari anggota Persadia yang hadir dan bersedia berpartisipasi. Pemeriksaan protein urin dan glukosa urin menggunakan carik celup. Penelitian dilaksanakan bulan Mei-November 2016. Hasil penelitian pada 40 sampel urin penderita DM tipe II anggota Persadia terdapat 8 orang (20%) dengan hasil protein urin positif  1, sedangkan 32 orang lainnya negatif. Hasil pemeriksaan glukosa urin 31 orang negatif, positif 1 pada 5 orang dan positif 4 pada 4 orang. Pada penderita DM tipe II ditemukan proteinuria dan glukosa dalam urin, sehingga penderita dengan proteinuria positip harus periksa ulang dalam 3-6 bulan untuk memantau fungsi ginjalnya.
Pengaruh Jumlah Pencucian Beras dengan Kadar Klorin Indah Purwaningsih; Supriyanto Supriyanto
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.102

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Abstract: Chlorine is a green halogen-shaped halogen gas at normal temperature and serves as bleach, stain remover and disinfectant. Chlorine is now widely used for bleaching rice so that less quality rice looks like quality rice. Chlorine is very toxic and causes mucous membrane irritation, highly reactive and very powerful oxidizer. The purpose of this research was to determine the difference of chlorine level in chlorinated rice washed once, twice and 3 times. The sample in this study amounted to 11 samples calculated by replication formula. Each sample was treated 3 times, ie 1 washed once, 2 washed twice and washed 3 times. The samples then examined by iodometric titration method. Based on the results of the study using ANOVA test, 11 samples obtained the average value of chlorine after washed once amount of 0.0176%, after washed twice amount of 0.0111%, and after washed 3 times amount of 0.0052% with the value significance p = 0.03 (p <0.05) at 95% confidence level which means there was a significant difference between chlorine levels in chlorinated rice washed once, twice and 3 times.Abstrak: Klorin merupakan unsur halogen berbentuk gas berwarna kuning kehijauan pada suhu normal danberfungsi sebagai pemutih, penghilang noda maupun desinfektan. Klorin sekarang banyak digunakan untuk bahan pemutih beras agar beras yang kurang berkualitas tampak seperti beras berkualitas. Klorin sangat toksik dan menyebabkan iritasi membran mukosa, sangat reaktif dan merupakan oksidator yang sangat kuat. Tujuan dari penelitian ini ialah untuk mengetahui perbedaan kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali. Sampel dalam penelitian ini berjumlah 11 sampel yang dihitung dengan rumus replikasi. Setiap sampel diberi perlakuan sebanyak 3 kali, yaitu 1 kali pencucian, 2 kali pencucian dan 3 kali pencucian. Sampel penelitian kemudian diperiksa dengan metode titrasi iodometri. Berdasarkan hasil penelitian menggunakan uji Anova secara komputerisasi terhadap 11 sampel diperoleh nilai rata-rata kadar klorin setelah 1 kali pencucian sebesar 0,0176 %, setelah 2 kali pencucian sebesar 0,0111 %, dan setelah 3 kali pencucian sebesar 0,0052 % dengan nilai signifkansi p = 0,03 (p<0,05) pada tingkat kepercayaan 95% yang artinya ada perbedaan yang bermakna antara kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali.