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Uji Efektivitas Perasan Buah Bintaro (Cerbera manghas) Terhadap Kematian Kecoa Amerika (Periplaneta americana) Barlian Barlian; Bagus Muhammad Ihsan; Novika Dewi Ana
Journal of Medical Laboratory Research Vol 1 No 1 (2022): Journal of Medical Laboratory Research
Publisher : Poltekkes Kemenkes Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.934 KB) | DOI: 10.36743/jomlr.v1i1.429

Abstract

Penelitian ini untuk mengetahui efektvitas perasan buah bintaro (Cerbera manghas) terhadap kematian kecoa amerika (Periplaneta americana). Hewan uji yang digunakan yaitu kecoa amerika berjumlah 180 ekor. Rancangan yang digunakan adalah rancang acak lengkap (RAL) dengan 5 perlakuan dan 5 pengulangan. Perlakuan variasi konsentrasi yang digunakan yaitu 15%, 30%, 45%, 60% dan 75%. Data yang diperoleh dianalisis menggunakan uji Kruskal wallis, hasil perhitungan uji Kruskal wallis menunjukkan Asym.Sig > 0.05 (0,076 > 0,05) artinya tidak terdapat jumlah kematian yang signifikan pada variasi konsentrasi perasan, Sehingga dapat disimpulkan bahwa perasan buah bintaro (Cerbera manghas) tidak efektif sebagai insektisida nabati terhadap kecoa amerika (Periplaneta americana) dan pada variasi konsentrasi perasan tertinggi yaitu 75% dapat mengakibatkan kematian 67% pada kecoa amerika (Periplaneta americana). Untuk penelitian lebih lanjut dapat menggunakan metode ekstraksi agar senyawa yang terkandung dalam tanaman dapat bekerja lebih optimal.
The Potential Antibacterial Effect of Papaya Leaf Extract (Carica papaya L) and Miana Leaf Extract (Coleus scutellarioides L) as Adjuvant Therapy for Rifampicin-Resistant Tuberculosis Wawan Sofwan Zaini; Nining Kurniati; Khayan Khayan; Bagus Muhammad Ihsan; Widyana Lakshmi Puspita; Muhammad Ifham Hanif
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 1 (2023): May
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i1.2044

Abstract

The adhesion of Rifampicin-resistant TB to neutrophils plays an essential role in colonization. Several active compounds in papaya leaf and Miana leaf (Coleus scutellarioides L) are believed to regulate or prevent the formation of bacterial colonies. The purpose of this study was to determine the anti-bacterial effectiveness of extracts of papaya leaf (Carica Papaya L.) and Miana leaf (Coleus scutellarioides L) against bacterial isolates of Rifampicin-Resistant TB strain (RR). This research method is a in vitro laboratory experiment, and extracts of papaya leaf and Miana leaf (50, 25, 12,5%) were tested as anti-bacterial using the M-TB susceptibility test using the Proportion Method. The results showed the anti-bacterial ability of papaya leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 0% so that it was included in the Sensitive category, but at a concentration of 25% the resistance percentage value was 42.86% % and a concentration of 12.5%, the percentage value of resistance is 42.86% so that it is included in the category of resistance (Resistant > 1% and Sensitive < 1%). The anti-bacterial ability of miana leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 5.33%, at a concentration of 25%, with a resistance percentage value of 17.14%, and at a concentration of 12.5%, with a resistance percentage value of 100%, so all are included in the resistant category. The Conclusion 50% papaya leaf extract inhibits the formation of Rifampicin-resistant MTBC-resistant bacterial colonies, allowing its usage as a substitute ingredient in Rifampicin-resistant MTBC-resistant medications.
IDENTIFIKASI DAN PROFIL RESISTENSI BAKTERI PADA MINUMAN AIR TAHU DAN AIR TEBU DI WILAYAH KOTA PONTIANAK Aditia Aditia; Ari Nuswantoro; Herlinda Djohan; Bagus Muhammad Ihsan; Nurul Uq'ma; Anisya Nur Shafitri; Affifatuzahara Affifatuzahara; Fahma Ajeng Al-qorifah; Benedikta Anjelica
Jurnal Ilmiah Global Education Vol. 4 No. 4 (2023): JURNAL ILMIAH GLOBAL EDUCATION, Volume 4 Nomor 4, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/jige.v4i4.1860

Abstract

Tofu and sugarcane juice drinks are in great demand because they taste delicious and affordable. These drinks can be contaminated with pathogenic bacteria due to hygiene and poor environment. Contamination of pathogenic bacteria that are resistant to antibiotics is a serious health threat.  The objective of this study is to identify and provide antibiotic resistance bacteria from tofu and sugarcane juice drinks overview. This Study is a descriptive-observation with a cross-sectional design. Bacterial identification is carried out by culture, while resistance testing is carried out by Kirby-Bauer. Penicillin, amoxicillin, ampicillin, ciprofloxacin, chloramphenicol, gentamicin, tetracycline, and vancomycin were used. 43 samples of tofu water drink and 70 samples of sugarcane juice drink were selected by purposive sampling. Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, and Klebsiella pneumoniae founds in tofu drinks, while Enterobacter aerogenes, Salmonella typhi, Shigella dysenteriae, and Escherichia coli founds in sugarcane juice. Staphylococcus aureus resistant to penicillin (100%), Salmonella typhi resistant to penicillin (66.6%), Enterobacter aerogenes resistant to tetracycline (70%), Klebsiella pneumoniae resistant to penicillin (100%), Shigella dysenteriae resistant to ampicillin and gentamicin (100%), and Escherichia coli resistant to vancomycin (100%). Pathogenic-resistant bacteria in tofu and sugarcane juice drinks show the risk of spreading resistant bacteria which can trigger bacterial resistance with a wider spectrum.
Profil Resistensi Bakteri Patogen Pada Minuman Air Tahu Di Wilayah Kota Pontianak Talen Nikita Sianturi; Wahdaniah Wahdaniah; Sugito Sugito; Bagus Muhammad Ihsan
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v3i6.6780

Abstract

Minuman air tahu adalah minuman yang dibuat dari kacang kedelai kuning, minuman ini berwarna putih kekuningan mirip dengan susu. Minuman ini banyak diminati oleh masyarakat Pontianak karena rasanya yang nikmat dan harganya yang terjangkau. Minuman tersebut dapat tercemar oleh bakteri patogen karena higiene penjual dan lingkungan yang kurang baik. Cemaran oleh minuman bakteri patogen yang resisten terhadap antibiotik merupakan ancaman kesehatan yang serius. Penelitian ini bertujuan untuk mengidentifikasi dan memberikan gambaran resistensi antibiotik yang diisolasi dari minuman air tahu. Penelitian berbentuk deskripsi observasi dengan metode kultur dan uji resistensi metode kirby-bauer. Antibiotik yang digunakan adalah penisilin, amoksilin, siprofloksasin, dan kloramfenikol. Jumlah sampel sebanyak 43 sampel. Hasil identifikasi ditemukan bakteri Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, dan Klebsiella pneumoniae. Hasil uji resitensi menunjukkan Staphylococcus aureus resisten terhadap penisilin (100%), Salmonella typhi resisten terhadap penisilin (66,6%), Enterobacter aerogenes resisten terhadap kloramfenikol (40%), dan Klebsiella pneumoniae resisten terhadap penisilin (100%).
Identifikasi Bakteri Gram Negatif Pada Air Tebu Di Wilayah Kota Pontianak Destia Aufani; Supriyanto Supriyanto; Imma Fatayati; Bagus Muhammad Ihsan
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v3i6.6781

Abstract

Air tebu merupakan minuman yang terbuat dari perasan batang tebu yang diperas dengan menggunakan mesin khusus membuatnya memiliki rasa yang manis alami, minuman ini sangat digemari oleh masyarakat. Akan tetapi kontaminasi mikroba pada air tebu sangat tinggi sehingga dapat menyebabkan berbagai penyakit. Berbagai bakteri dari genus Escherichia, Salmonella, Klebsiella, Shigella, dan Enterobacter yang merupakan salah satu indikator polusi limbah dan kondisi yang tidak menguntungkan untuk makanan dan minuman. Penelitian ini bertujuan untuk mengetahui bakteri yang terdapat pada minuman air tebu di Kota Pontianak. Penelitian ini menggunakan beberapa sampel air tebu di wilayah kota Pontianak dengan menggunakan metode kultur. Hasil yang didapatkan dari 70 sampel yaitu mendapatkan 8 sampel positif bakteri Enterobacter aerogenes, 4 sampel positif bakteri Salmonella typhi, 14 sampel positif Klebsiella pneumonia, 6 sampel positif Shigella dysentriae, dan 15 sampel positif Escherichia coli.
PENDAYAGUNAAN LUMPUR GAMBUT DAN KOTORAN SAPI UNTUK MEMPERCEPAT COMPOSTING PADA SAMPAH RUMAH TANGGA Bagus Muhammad Ihsan; Khayan Khayan; Cecep Dani Sucipto; Widyana Lakshmi Puspita; Nasihin Nasihin
Jurnal Abdi Insani Vol 11 No 1 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i1.1425

Abstract

Environmental pollution-based diseases, nationally like ISPA, malnutrition, diarrhea and thypus are still high. These diseases can be transmitted through disposal of local households and farms that are naturally disposed of (traditionally) unhealthy, such as open dumping, irrigation and dumping in wells. (parit). To control parasites such as helminths and their vectors, health technology is needed, especially for the use of domestic garbage and local cattle debris feces for composting. In addition to serving for the fertilization of crops, the production of composting also added economic value to farmers' incomes and improved public health, as well as the reduction of germs and parasite diseases. The production of composting of household garbage and livestock food residues can be accelerated using cabbage mud and cattle feces. The use of crab mud can accelerate such composting processes, because in cabbag mud contains microbiological Bacilus sp. aerobic/ anaerobic cutting agents. Adding cattle dirt to the composting process can be useful in accelerating the biodegradation process of household garbage. This ability is due to microorganisms such as protozoa, function, Streptococcus sp. and cellulolithic bacteria, which produce cellulose that can break down (biodegradate) the whole substance in household garbage. The purpose of dedication to the community is to expect the cadres and the community to use the mud and feces of cattle to make compost fertilizer. Research methods use Observational and Socialization to the variables studied. The result of dedication to this community is increasing knowledge about cabbage and cow dirt to accelerate composting on household garbage as well as obtaining compost fertilizer for the community.
The Effect of Bay Leaf (Syzygium Polyanthum) Extract Hand Washing Liquid Soap Preparation on The Growth of Escherichia Coli Bacteria in Vitro Wahdaniah; Asep Sabolakna; Bella Ramadhanti; Bagus Muhammad Ihsan; Yeremiah Rubin Tjamin
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7175

Abstract

Bay leaf (Syzygium polyanthum) is known as a complement to cooking spices, also used by the community for treatment. The content of antibacterial compounds from bay leaves such as alkaloids, flavonoids, saponins and tannins which can inhibit bacterial growth. This study aims to determine the effect of hand washing liquid soap with bay leaf extract on the growth of Escherichia coli bacteria. This study uses a Quasi Experiment research design. The population in this study is located on Jalan Atot Ahmad, West Pontianak District. The sample used was liquid hand washing soap with bay leaf extract at a concentration of 3, 6, and 9%, 9 repetitions with a total of 27 samples, using the disc diffusion method. The results of the antibacterial study showed that the average diameter of the inhibition zone at 3% concentration was 8.27 mm, 6% concentration was 10.55 mm and 9% concentration was 11.16. The results of the Linear Regression test using the Anova test obtained pvalue = 0.000, which is pvalue < α, with α = 0.05, so that it was stated that H1 was accepted so that there was an effect on the preparation of liquid hand washing soap (Syzygium polyanthum) extract on the growth of Escherichia coli bacteria in vitro.
Influence pH to Rate Anthocyanins Extract Senggani Fruit ( Melastoma malabathricum Auct, non Linn) Ratih Indrawati; Gervacia Jenny.R; Maulidiyah Salim; Bagus Muhammad Ihsan; Ari Widiyantoro
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7267

Abstract

One of the plants that can be used as a natural dye is senggani fruit. This study aimed to determine the effect of acid pH on the anthocyanin levels of the senggani fruit extract. The method used in this study uses UV-Vis Spectrophotometry. The sample was senggani fruit extract, added with an acidic pH solution (1-6) with 3 repetitions for 18 samples. Based on the results of research that has been carried out, the average anthocyanin levels at pH 1 are 2.00% with a bright red color; pH 2 is 1.63% with a red-orange color, pH 3 is 1.45% with an orange color, pH 4 is 2.05 % with brownish red color, pH 5 is 1.38% with brownish orange color, and pH 6 is 1.11% with light brown color. Based on statistical tests using Simple Linear Regression, Ha was accepted so that it could be concluded that there was an effect of acid pH on the anthocyanin levels of the senggani fruit. Based on a study that has been done, it is known that the sourer the pH solution is, the taller the anthocyanin obtained. Matter happens Because absorbance is comparable with the rate of anthocyanin something sample.
Gambaran Kadar Glukosa Darah Sewaktu Pada Ibu Hamil di Puskesmas Tambelan Sampit Kota Pontianak Aditiya Ramadan; Ari Nuswantoro; Linda Triana; Bagus Muhammad Ihsan
Jurnal Ners Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v8i1.22203

Abstract

characterised by high blood sugar levels as well as problems with protein, lipid, and carbohydrate metabolism as a result of inadequate insulin activity. Patients who have wounds infected by pathogenic bacteria, both Gram positive and Gram negative, run the risk of developing a number of problems, including neuropathy, hypertension, coronary heart disease, retinopathy, nephropathy, and gangrene. This cross-sectional, observational, descriptive study aims to pinpoint pathogenic bacteria in the diabetic patients' wounds at the diabetes wound care clinic in Pontianak City. The bacterial identification stage includes inoculation on enrichment media, Gram staining, inoculation and observation of colonies on differential-selective media, and biochemical tests. Examination results showed that Klebsiella pneumoniae was identified in 20 samples (57%), Pseudomonas aeruginosa in 8 samples (23%), and Staphylococcus aureus in 7 samples (20%). According to the study, diabetic wound problems put DM patients at risk of contracting dangerous microorganisms and spreading them to others. In order to prevent infection and other health issues, patients are instructed on how to properly care for their wounds. They are also provided the correct follow-up by medical professionals.
Soap Made from Kesum Leaf Squeezedto Inhibit Growth Staphylococcus aureus. Bagus Muhammad Ihsan; Elisabeth Lusitania; Sugito
Jurnal teknologi Kesehatan Borneo Vol 4 No 2 (2023): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v4i2.271

Abstract

Biodiversity, especially in West Kalimantan, is very diverse, one of which is the kesum plant (Polygonum minus husks). The kesum plant is a plant that can be used as herbal medicine. The utilization of kesum plants is usually done as part of the leaves, namely as local processed cooking spices. Based on Fitofarmaka studies, kesum plants are believed to have antibacterial, antifungal, anti-viral, antioxidant, anti-cancer, and antiulcer activities. The study's aim was to find out how well different concentrations of kesum leaf juice-based solid soap formulations stopped the growth of Staphylococcus aureus bacteria. This study used kesum leaves (Polygonum minus husks), which were squeezed, and then made solid soap preparations with concentrations of 3.93%, 5.25%, and 6.56%. Nine repetitions were carried out in each treatment, so the number of samples used was 27. The results of the antibacterial inhibition test showed that the soap preparation samples had the ability to inhibit the growth of Staphylococcus aureus bacteria. The result of the statistical analysis value in the Friedman test is P = 0.000, which states that there is a difference in each concentration of solid soap prepared from kesum leaf juice. Dilution concentrations of 5.25% and 6.56% are more effective in inhibiting the growth of Staphylococcus aureus bacteria than 3.93%.